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MUC1兔單克隆抗體

MUC1兔單克隆抗體

  • 價(jià)格¥600
  • 規(guī)格100ul
  • 貨號HKZ150152
  • 單位
  • 品牌HaoKebio

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HaoKebioHKZ150152MUC1兔單克隆抗體100ul600
MUC?1
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# ?BX50029?Clone# ?BP6034
Predicted?Molecular Wt:
122kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P15941
?

Background:
MUC1?(Mucin?1)?is a transmembrane glycoprotein that?protects epithelial?cells?from?injury?caused?by?external?stimuli.?In????addition to this?role,?MUC1?is?involved?in cell-cell adhesion,?proliferation,?motility,?invasion and?survival.?In?epithelial?cells,?MUC1 expression?is?regulated?by?binding of TNFα to TNFR1 and?activation?of the?NFκB?pathway.
?
MUC1?is?present?in a variety of glandular?(secretory) epithelia?such?as?breast,?eccrine?and?apocrine?glands,?and?pancreas,
whereas?little or?no?MUC1?is expressed?in the gastrointestinal epithelium,?endocervical?epithelium,?and?prostate?glands. The???immunoreactivity?is?usually?limited to apical cell?membranes,?but a?staining?of the?Golgi?zone?may?also?be?seen.?MUC1?can?be?demonstrated?in?most types of adenocarcinomas derived from secretory?epithelia.?In?well-differentiated?carcinomas,?the
staining?is?mostly?limited to?(apical) cell?membranes while?in the?less differentiated carcinomas,a?cytoplasmic?staining?is?seen?with?loss of staining?polarity?in the?membranes.
?
MUC1 was?used to?recognize epithelial differentiation when?cytokeratin was?difficult?to?visualize.?In?the?classification?of
haematolymphoid?neoplasms, a?positive?MUC1?reaction?may?be an aid?in the?identification?of diffuse?large?B-cell?lymphoma,?plasma cell?neoplasms and?nodular?lymphocyte?predominant?Hodgkin’s?lymphoma.
?

Subcellular?location:
Membrane/Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?MUC1?residues?within?aa1155-1255 of?MUC1 was?used?as?an
immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Immunohistochemistry
(Formalin/PFA-fixed
paraffin-embedded
sections?analysis of?human?ovarian cancer?tissue
labelling?MUC1 with
BP6034.?Heat?mediated antigen?retrieval?was
performed?using Tris/EDTA?buffer?pH?9.0

Background?References:
1.?Croce?MV, et.al, J?Histochem?Cytochem.?2003?Jun;51(6):781-8.
2.??NakamoriS, et.al, Gastroenterology.?1994?Feb;106(2):353-61.

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