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OCT4鼠單克隆抗體

OCT4鼠單克隆抗體

  • 價(jià)格¥1960
  • 規(guī)格100ul
  • 貨號(hào)HK510133M
  • 單位
  • 品牌HaoKebio

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HaoKebioHK510133MOCT4鼠單克隆抗體100ul1960

Applications

Tested Applications:

IHC, WB,ELISA


Cited Applications:

IHC, WB


Species Specificity:

human, mouse, rat


Cited Species:

human, mouse


Positive WB detected in:

HEK-293 cells, NCCIT cells, Neuro-2a cells, HT-29 cells, human brain tissue


Positive IHC detected in:

human breast cancer tissue, human colon cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0


Recommended dilution:

WB : 1:5000-1:20000

IHC : 1:250-1:1000

Product Information


Source:

Mouse


Purification method:

Protein A purification


Isotype:

IgG1


Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:

POU class 5 homeobox 1


Calculated molecular weight:

39 kDa


Observed molecular weight:

50-52 kDa


Gene symbol

POU5F1


Synonyms

Oct 3, Oct 4, OCT3, OCT3/4, OCT4, Octamer binding protein 3, Octamer binding protein 4, OTF 3, OTF3, OTF4, POU class 5 homeobox 1, POU5F1

Background

Expression of the OCT4 transcription factor is associated with an undifferentiated phenotype of human and mouse embryonic stem cells and germ cells. OCT4 (Octamer-4) is a homeodomain transcription factor of the POU family (POU5F1) involved in regulation of pluripotency during normal development and is detectable in embryonic stem (ES) and germ cells. Its expression is associated with an undifferentiated phenotype and tumors. In fact, gene knockdown of OCT4 promotes differentiation, thereby demonstrating a role for this factor in human ES cell self-renewal. As such, it is frequently used as a marker for undifferentiated ES cells or iPS (induced pluripotent stem) cells. OCT4 is one of the four key transcription factors (OCT4, SOX2, c-MYC, and KLF4) used to reprogram mouse and human fibroblasts to a pluripotent state as well as generate patient-specific iPS cells . The calculated molecular weight of OCT4 is 38 kDa, but the modified OCT4 protein is about 50 kDa.

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