{"id":9456,"date":"2024-09-21T10:08:40","date_gmt":"2024-09-21T02:08:40","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=9456"},"modified":"2024-09-23T14:24:23","modified_gmt":"2024-09-23T06:24:23","slug":"%e5%bf%ab%e9%80%9f%e5%8e%bb%e5%9f%ba%e5%9b%a0%e7%bb%84%e9%80%86%e8%bd%ac%e5%bd%95%e9%a2%84%e6%b7%b7%e6%b6%b2","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/9456.html","title":{"rendered":"\u5feb\u901f\u53bb\u57fa\u56e0\u7ec4\u9006\u8f6c\u5f55\u9884\u6df7\u6db2"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"\u5feb\u901f\u53bb\u57fa\u56e0\u7ec4\u9006\u8f6c\u5f55\u9884\u6df7\u6db2\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">\u5feb\u901f\u53bb\u57fa\u56e0\u7ec4\u9006\u8f6c\u5f55\u9884\u6df7\u6db2\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/sjfzswx\/hkpcr\/\">PCR<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/sjfzswx\/\">\u5206\u5b50\u751f\u7269\u5b66<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe5690<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100 rxns<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKR041<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u652f<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">\u6d69\u514b<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p><strong>\u670d\u52a1\u4ecb\u7ecd\uff1a<\/strong><\/p>\n\n\n\n<figure style=\"font-size:15px\" class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>\u7ec4\u5206<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ea7\u54c1\u540d\u79f0<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u89c4\u683c<\/strong><\/td><\/tr><tr><td>HKR041-A<\/td><td class=\"has-text-align-center\" data-align=\"center\">All-in-One First-Strand Synthesis MasterMix<\/td><td class=\"has-text-align-center\" data-align=\"center\">400 \u03bcL<\/td><\/tr><tr><td>HKR041-B<\/td><td class=\"has-text-align-center\" data-align=\"center\">dsDNase<\/td><td class=\"has-text-align-center\" data-align=\"center\">2\u00d750 \u03bcL<\/td><\/tr><tr><td>HKR041-C<\/td><td class=\"has-text-align-center\" data-align=\"center\">10\u00d7 dsDNase Buffffer<\/td><td class=\"has-text-align-center\" data-align=\"center\">200 \u03bcL<\/td><\/tr><tr><td>HKR041-D<\/td><td class=\"has-text-align-center\" data-align=\"center\">Nuclease-Free Water<\/td><td class=\"has-text-align-center\" data-align=\"center\">2\u00d71 mL<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br><h4><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;\nmso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:normal;mso-bidi-font-weight:bold;\nfont-size:12.0000pt;mso-font-kerning:1.0000pt;\"><font face=\"\u9ed1\u4f53\">\u3010<\/font><\/span><span style=\"font-size: 15px; color: initial;\">\u4ea7\u54c1\u7b80\u4ecb\u3011<\/span><\/h4><p class=\"MsoNormal\">\u5feb\u901f\u53bb\u57fa\u56e0\u7ec4\u9006\u8f6c\u5f55\u9884\u6df7\u6db2\uff08All-in-One First-Strand Synthesis MasterMix (with dsDNase)\uff09\u662f\u4e00\u6b3e\u9ad8\u6548\u3001\u4fbf\u6377\u3001\u51cf\u5c11\u6c61\u67d3\u7684\u9ad8\u8d28\u91cf\u4e00\u94fe cDNA \u5408\u6210\u8bd5\u5242\u76d2\uff0c\u5305\u542bM-MLV GIII Reverse Transcriptase \u53ca\u5176\u53cd\u5e94 Buffer\u3001RNA \u9176\u6291\u5236\u5242\u3001dNTPs, Oligo(dT)20VN \u548c\u968f\u673a\u5f15\u7269\u7b49\u4e00\u94fe cDNA \u5408\u6210\u6240\u9700\u7684\u6240\u6709\u7ec4\u5206\uff0c\u4ec5\u9700\u52a0\u5165 RNA \u6a21\u677f\u548c\u6c34\u5373\u53ef\u5f00\u59cb\u53cd\u5e94\u3002\u4f7f\u7528\u8be5\u9006\u8f6c\u5f55\u8bd5\u5242\u76d2\u83b7\u5f97\u7684 cDNA\uff0c\u4e0b\u6e38\u53ef\u7528\u4e8e qPCR\u3001\u666e\u901a PCR \u7b49\u5b9e\u9a8c\u3002<\/p><br><p class=\"MsoNormal\"><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;font-size:12.0000pt;\nmso-font-kerning:1.0000pt;\"><\/span>RNA \u4e2d\u5b58\u5728\u57fa\u56e0\u7ec4 DNA \u6c61\u67d3\uff0c\u5982\u679c\u53cd\u8f6c\u5f55\u524d\u4e0d\u505a\u53bb\u9664\u5904\u7406\uff0c\u4e0b\u6e38\u8fdb\u884c qPCR \u53cd\u5e94\u65f6\u57fa\u56e0\u7ec4 DNA \u4e0e cDNA \u4f1a\u540c\u65f6\u8fdb\u884c\u6269\u589e\uff0c\u5c24\u5176\u662f\u5f15\u7269\u8bbe\u8ba1\u5728\u540c\u4e00\u5916\u663e\u5b50\u4e0a\u65f6\u3002\u672c\u8bd5\u5242\u76d2\u91c7\u7528 dsDNase \u9ad8\u6548\u53bb\u9664\u57fa\u56e0\u7ec4 DNA \u6c61\u67d3\uff0c\u533a\u522b\u4e8e\u5e38\u89c4\u7684 DNase I\uff0cdsDNase \u80fd\u591f\u7279\u5f02\u6027\u7684\u6d88\u5316\u53cc\u94fe DNA\uff08dsDNA \u4ee5\u53ca DNA \u4e0e RNA \u7684\u6742\u5408\u94fe\uff09\uff0c\u5e76\u4e14\u5177\u6709\u70ed\u654f\u611f\u6027\uff0c\u53ef\u5728\u9ad8\u6e29\u6761\u4ef6\u4e0b\u5feb\u901f\u4e0d\u53ef\u9006\u5730\u5931\u6d3b\u3002\u4e0e\u4f20\u7edf\u4f7f\u7528 DNase I \u53bb\u9664\u57fa\u56e0\u7ec4 DNA \u6c61\u67d3\u7684\u65b9\u6cd5\u76f8\u6bd4\uff0cdsDNase \u65e0\u9700\u989d\u5916\u52a0\u5165 EDTA \u8fdb\u884c\u5931\u6d3b\uff0c\u4e0d\u4ec5\u8282\u7701\u5b9e\u9a8c\u65f6\u95f4\uff0c\u800c\u4e14\u964d\u4f4e\u4e86\u5bf9\u9006\u8f6c\u5f55\u53cd\u5e94\u7684\u6291\u5236\u3002All-in-One First-Strand Synthesis MasterMix (with dsDNase) \u4f5c\u4e3a\u5347\u7ea7\u540e\u7684\u4e00\u94fe cDNA \u5408\u6210\u8bd5\u5242\u76d2\uff0c15 \u5206\u949f\u5185\u6700\u957f\u53ef\u83b7\u5f97 12 kb cDNA\u3002\u53ef\u4f9d\u636e\u57fa\u56e0\u7ec4\u6c61\u67d3\u4e25\u91cd\u7a0b\u5ea6\u9009\u62e9\u91c7\u7528\u53bb\u57fa\u56e0\u7ec4 DNA \u6c61\u67d3\u4e0e\u53cd\u8f6c\u5f55\u5206\u5f00\u8fdb\u884c\u7684\u64cd\u4f5c\u65b9\u6cd5\uff0c\u6216\u8005\u53bb\u57fa\u56e0\u7ec4\u6c61\u67d3\u4e0e\u53cd\u8f6c\u5f55\u4e00\u6b65\u6cd5\u8fdb\u884c\u7684\u64cd\u4f5c\u65b9\u6cd5\u3002<span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;font-size:12.0000pt;\nmso-font-kerning:1.0000pt;\"><\/span><font face=\"\u5b8b\u4f53\"><\/font><span style=\"mso-spacerun:'yes';font-family:\u5b8b\u4f53;font-size:12.0000pt;\nmso-font-kerning:1.0000pt;\"><\/span><\/p><strong><br>\u8be6\u7ec6\u4fe1\u606f\u8bf7\u53c2\u8003\u8bf4\u660e\u4e66\uff01<\/strong><h4><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;\nmso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:normal;mso-bidi-font-weight:bold;\nfont-size:12.0000pt;mso-font-kerning:1.0000pt;\"><\/span><span style=\"mso-spacerun:'yes';font-family:Arial;mso-fareast-font-family:\u9ed1\u4f53;\nmso-bidi-font-family:'Times New Roman';mso-ansi-font-weight:normal;mso-bidi-font-weight:bold;\nfont-size:12.0000pt;mso-font-kerning:1.0000pt;\"><\/span><span style=\"margin: 0px; padding: 0px; list-style: none; box-sizing: border-box; -webkit-tap-highlight-color: transparent; letter-spacing: 0px;\"><\/span><\/h4><\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-290b266c-8953-4864-afc4-e63cbcd309f0\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20240921100824333.pdf\">All-in-One First-Strand Synthesis MasterMix (with dsDNase) HKR041 \u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20240921100824333.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-290b266c-8953-4864-afc4-e63cbcd309f0\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p>\u65e0<\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":7870,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[271,235,98],"tags":[],"class_list":["post-9456","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-hkpcr","category-sjfzswx","category-products"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"catce":["sidebar-widgets4"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:6:\"\uffe5690\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u89c4\u683c\";s:4:\"text\";s:8:\"100 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