{"id":9270,"date":"2024-09-14T16:43:13","date_gmt":"2024-09-14T08:43:13","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=9270"},"modified":"2024-10-25T09:03:25","modified_gmt":"2024-10-25T01:03:25","slug":"western-blot%e5%b0%81%e9%97%ad%e6%b6%b2","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/9270.html","title":{"rendered":"Western Blot\u5c01\u95ed\u6db2"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"Western Blot\u5c01\u95ed\u6db2\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">Western Blot\u5c01\u95ed\u6db2\n\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/wb\/fengbi\/\">\u5c01\u95ed<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/wb\/\">\u86cb\u767d\u8d28\u751f\u7269\u5b66<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a5120<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100ml<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKW2100<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u74f6<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\"><\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n\n\n\n<p style=\"font-size:15px\"><strong>\u3010\u4ea7\u54c1\u4fe1\u606f\u3011<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\" style=\"font-size:15px\"><table class=\"has-fixed-layout\"><tbody><tr><td>\u4ea7\u54c1\u540d\u79f0<\/td><td>\u4ea7\u54c1\u8d27\u53f7<\/td><td>\u89c4\u683c<\/td><td>\u6709\u6548\u671f<\/td><\/tr><tr><td>Western Blot\u5c01\u95ed\u6db2<\/td><td>HKW2100<\/td><td>100mL\/500mL<\/td><td>\u4e00\u5e74<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<h4 class=\"wp-block-heading\" style=\"font-size:15px\">\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/h4>\n\n\n\n<p style=\"font-size:15px\">&nbsp;\u672c\u4ea7\u54c1\u662f\u65e0\u86cb\u767dWestern Blot\u5c01\u95ed\u6db2\uff0c\u4e3a\u591a\u79cd\u805a\u5408\u7269\u7684\u6df7\u5408\u6db2\uff0c\u53ef\u5feb\u901f\u586b\u5145\u56fa\u76f8\u652f\u6301\u7269\u8868\u9762\u7684\u7a7a\u9699\uff0c\u51cf\u5c11\u975e\u7279\u5f02\u6027\u4fe1\u53f7\u7684\u5e72\u6270\uff1b\u6574\u4f53\u5c01\u95ed\u6548\u679c\u660e\u663e\u4f18\u4e8e\u4f20\u7edf\u7684\u57fa\u4e8eBSA\uff08\u725b\u8840\u6e05\u767d\u86cb\u767d\uff09\u3001\u8131\u8102\u5976\u7c89\u3001\u916a\u86cb\u767d\uff08Casein\uff09\u7b49\u7684\u5c01\u95ed\u6db2\uff1b\u53ef\u7528\u4e8e Western Blot\uff08WB\uff09\u7684\u5b9e\u9a8c\u4e2d\u7684\u5c01\u95ed\u3001\u4e00\u6297\u7a00\u91ca\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">   <strong>\u5feb\u901f\u9ad8\u6548<\/strong>\uff1a\u5c01\u95ed\u65f6\u95f45-10\u5206\u949f\u5373\u53ef\uff0c\u5e76\u4e14\u548cBSA\u3001\u8131\u8102\u5976\u7c89\u3001\u916a\u86cb\u767d\u7b49\u4f20\u7edf\u5c01\u95ed\u5242\u76f8\u6bd4\uff0c\u7279\u5f02\u6027\u4fe1\u53f7\u66f4\u5f3a\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">   <strong>\u80cc\u666f\u4f4e<\/strong>\uff1a\u672c\u5c01\u95ed\u6db2\u4e0d\u542b\u8840\u6e05\u3001\u767d\u86cb\u767d\u7b49\u86cb\u767d\u8d28\uff0c\u786e\u4fdd\u4e86\u6781\u9ad8\u7684\u4fe1\u566a\u6bd4\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">   <strong>\u517c\u5bb9\u6027\u597d<\/strong>\uff1a\u672c\u4ea7\u54c1\u517c\u5bb9\u8fa3\u6839\u8fc7\u6c27\u5316\u7269\u9176\uff08HRP\uff09\u3001\u78b1\u6027\u78f7\u9178\u9176(AP)\u548c\u751f\u7269\u7d20\u6807\u8bb0\u7684\u4e8c\u6297\uff1b\u4e0d\u542b\u5f71\u54cd HRP \u548cAP \u6d3b\u6027\u7684\u9632\u8150\u5242\uff1b\u56e0\u4e0d\u542b\u751f\u7269\u7d20\uff0c\u6545   \u4e0d\u4f1a\u5e72\u6270\u57fa\u4e8e\u751f\u7269\u7d20\u7684\u68c0\u6d4b\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">   <strong>\u4f7f\u7528\u4fbf\u6377<\/strong>\uff1a\u672c\u4ea7\u54c1\u542b TBST\uff0c\u53ef\u76f4\u63a5\u7528\u4e8e\u4e00\u6297\u7684\u7a00\u91ca\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<h4 class=\"wp-block-heading\" style=\"font-size:15px\">\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/h4>\n\n\n\n<p style=\"font-size:15px\">&nbsp;&nbsp;&nbsp;\u51b0\u888b\uff08wet ice\uff09\u8fd0\u8f93\uff1b4\u2103\u4fdd\u5b58\uff0c12 \u4e2a\u6708\u6709\u6548\uff0c\u957f\u671f\u4e0d\u7528\u53ef-20\u2103\u4fdd\u5b58\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<h4 class=\"wp-block-heading\" style=\"font-size:15px\">\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/h4>\n\n\n\n<p style=\"font-size:15px\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;1.&nbsp;\u5b8c\u6210\u8f6c\u819c\u540e\u7528 TBST \u6d17\u6da4 1-2 \u5206\u949f \uff1b<\/p>\n\n\n\n<p style=\"font-size:15px\">&nbsp;&nbsp;2.&nbsp;\u6839\u636e\u819c\u7684\u5927\u5c0f\uff0c\u9009\u53d6\u5408\u9002\u7684\u5bb9\u5668\uff0c\u52a0\u5165\u4e00\u5b9a\u4f53\u79ef\u7684\u65e0\u86cb\u767d\u5feb\u901f\u5c01\u95ed\u6db2\uff0c\u786e\u4fdd\u5c01\u95ed\u6db2\u80fd\u5b8c\u5168\u8986\u76d6\u819c\uff0c\u7f6e\u4e8e\u949f\u6446\u6216\u6c34\u5e73\u6447\u5e8a\u4e0a\u5c01\u95ed 5-10&nbsp;\u5206\u949f\uff08\u82e5\u51fa\u73b0\u9ad8\u80cc\u666f\uff0c\u5ef6\u957f\u5c01\u95ed\u65f6\u95f4\uff09\uff1b<\/p>\n\n\n\n<p style=\"font-size:15px\">&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.\u4f7f\u7528\u65e0\u86cb\u767d\u5feb\u901f\u5c01\u95ed\u6db2\u7a00\u91ca\u4e00\u6297\uff0c\u7a00\u91ca\u500d\u6570\u53c2\u8003\u5bf9\u5e94\u4e00\u6297\u4f7f\u7528\u8bf4\u660e\u4e66\uff0c\u5236\u6210\u4e00\u6297\u5de5\u4f5c\u6db2\uff1b<\/p>\n\n\n\n<p style=\"font-size:15px\">&nbsp;&nbsp;4.&nbsp;\u5c01\u95ed\u5b8c\u6210\u540e\u5012\u51fa\u65e0\u86cb\u767d\u5c01\u95ed\u6db2\uff0cTBST \u6e05\u6d17 10 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style=\"font-size:15px\">\u7531\u4e8e\u6ca1\u6709\u4efb\u4f55\u4e00\u79cd\u5c01\u95ed\u6db2\u662f\u9002\u7528\u4e8e\u6240\u6709\u5b9e\u9a8c\u4f53\u7cfb\u7684\uff0c\u56e0\u6b64\u5bf9\u4e8e\u4e00\u4e9b\u7279\u6b8a\u7684\u5b9e\u9a8c\uff0c\u53ef\u80fd\u9700\u8981\u6839\u636e\u5177\u4f53\u60c5\u51b5\u8003\u8651\u4f7f\u7528\u5176\u5b83\u66f4\u5408\u9002\u7684\u5c01\u95ed\u6db2\u3002<\/li>\n\n\n\n<li style=\"font-size:15px\">\u53d6\u653ePVDF\u819c\u548cNC\u819c\u5e94\u4f7f\u7528\u5e73\u5934\u954a\u5b50\uff0c\u5e76\u4ec5\u8f7b\u8f7b\u5939\u53d6\u5176\u8fb9\u89d2\uff0c\u64cd\u4f5c\u8fc7\u7a0b\u987b\u907f\u514d\u819c\u8868\u9762\u4ea7\u751f\u5212\u75d5\u3001\u6298\u75d5\u6216\u538b\u75d5\u7b49\u75d5\u8ff9\u3002<\/li>\n\n\n\n<li 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