{"id":9213,"date":"2024-09-14T10:11:58","date_gmt":"2024-09-14T02:11:58","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=9213"},"modified":"2024-10-09T17:14:55","modified_gmt":"2024-10-09T09:14:55","slug":"edu%ef%bc%885-%e4%b9%99%e7%82%94%e5%9f%ba-2-%e8%84%b1%e6%b0%a7%e5%b0%bf%e5%98%a7%e5%95%b6%e6%a0%b8%e8%8b%b7%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/9213.html","title":{"rendered":"EdU\uff085-\u4e59\u7094\u57fa-2&#8242;-\u8131\u6c27\u5c3f\u5627\u5576\u6838\u82f7\uff09"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"EdU\uff085-\u4e59\u7094\u57fa-2'-\u8131\u6c27\u5c3f\u5627\u5576\u6838\u82f7\uff09\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">EdU\uff085-\u4e59\u7094\u57fa-2&#8242;-\u8131\u6c27\u5c3f\u5627\u5576\u6838\u82f7\uff09\n\n\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/cpzh\/\">\u5206\u5b50\u75c5\u7406<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/cpzh\/hkygrl\/\">\u8367\u5149\u67d3\u6599<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a5650<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100mg<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKI0024<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u652f<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><th>\u54c1\u724c<\/th><th>\u5546\u54c1\u7f16\u7801<\/th><th>\u5546\u54c1\u7f16\u7801<\/th><th>\u89c4\u683c<\/th><th>\u4ef7\u683c<\/th><\/tr><tr><th><span style=\"color: #333333;\">HaoKebio<\/span><\/th><th>HKI0024<\/th><th>EdU\uff085-\u4e59\u7094\u57fa-2&#8242;-\u8131\u6c27\u5c3f\u5627\u5576\u6838\u82f7\uff09<\/th><th>100mg<\/th><th>\uffe5650<\/th><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><br>EdU\uff085-\u4e59\u7094\u57fa-2&#8242;-\u8131\u6c27\u5c3f\u5627\u5576\u6838\u82f7\uff09\u662f\u4e00\u79cd\u542b\u6709\u4e00\u4e2a\u4e59\u7094\u57fa\u56e2\u7684\u80f8\u817a\u5627\u5576\u8131\u6c27\u6838\u82f7\u7c7b\u4f3c\u7269\u3002EdU \u53ef\u7528\u4e8e\u52a8\u7269\u6d3b\u4f53\u6ce8\u5c04\uff0c\u5bf9\u751f\u7269\u4f53\u65e0\u660e\u663e\u526f\u4f5c\u7528\u4e14\u7a33\u5b9a\u6027\u8f83\u597d\uff0c\u5f53\u5c06\u5176\u6ce8\u5c04\u5230\u52a8\u7269\u4f53\u5185\uff0c\u8fd9\u4e9b\u5c0f\u5206\u5b50\u5728\u52a8\u7269\u4f53\u5185\u80fd\u591f\u8fc5\u901f\u6269\u6563\u5230\u5404\u4e2a\u5668\u5b98\u7ec4\u7ec7\uff0c\u5e76\u6e17\u5165\u5230\u7ec6\u80de\u4e2d\uff0c\u5728\u7ec6\u80de\u589e\u6b96\u65f6\u671f\u4ee3\u66ff\u80f8<br>\u82f7\uff08T\uff09\u63ba\u5165\u5230\u65b0\u5408\u6210\u7684 DNA \u4e2d\uff0c\u968f\u540e\u53ef\u5c06\u76ee\u6807\u7ec4\u7ec7\u5236\u5907\u4e3a\u77f3\u8721\u6216\u51b0\u51bb\u7ec4\u7ec7\u5207\u7247\u540e\u68c0\u6d4b\uff1bEdU \u4e5f\u9002\u7528\u4e8e\u4f53\u5916\u57f9\u517b\u7684\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\uff0c\u9002\u7528\u4e8e\u5c0f\u9f20\uff0c\u5927\u9f20\u53ca\u5176\u4ed6\u52a8\u7269\u6a21\u578b\u7684\u5404\u79cd\u7ec4\u7ec7\u5668\u5b98(\u8840\u7ba1\u9664\u5916)\u7684 EdU \u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u3002EdU \u5206\u5b50\u4e2d\u7684\u4e59\u7094\u57fa\u56e2\u80fd\u4e0e\u8367\u5149\u6807\u8bb0\u7684\u53e0\u6c2e\u5316\u5408\u7269\u63a2\u9488\u5728\u94dc\u79bb\u5b50\u50ac\u5316\u4e0b\u53d1\u751f\u201c\u70b9\u51fb\u201d\u53cd\u5e94\u5f62\u6210\u7a33\u5b9a\u7684\u4e09\u5511\u73af\uff0c\u56e0\u6b64\u53ef\u4ee5\u4f7f\u65b0\u5408\u6210\u7684 DNA \u88ab\u76f8\u5e94\u7684\u8367\u5149\u63a2\u9488\u6240\u6807\u8bb0\u3002<br>EdU \u68c0\u6d4b\u6cd5\u540c\u653e\u5c04\u6027\u6807\u8bb0\u6838\u82f7\u63ba\u5165\u6cd5\u76f8\u6bd4\uff0c\u6ca1\u6709\u653e\u5c04\u6027\u6c61\u67d3\u7b49\u9650\u5236\u56e0\u7d20\uff1b\u540c BrdU \u68c0\u6d4b\u6cd5\u76f8\u6bd4\uff0cEdU \u68c0\u6d4b\u6cd5\u4e0d\u9700\u8981 DNA \u53d8\u6027\u5904\u7406\uff0c\u4e5f\u4e0d\u4f9d\u8d56\u4e8e\u6297\u539f\u6297\u4f53\u53cd\u5e94\uff0c\u8fd9\u5927\u5927\u51cf\u5c11\u4e86\u5b9e\u9a8c\u7684\u590d\u6742\u6027\u548c\u64cd\u4f5c\u65f6\u95f4\u3002EdU \u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u6cd5\u5df2\u88ab\u5e7f\u6cdb\u5e94\u7528\u5230\u4f53\u5916\u7ec6\u80de\u6216\u7ec4\u7ec7\u7ec6\u80de\u589e\u6b96\u68c0\u6d4b\u4e2d\u3002<br><br><strong>\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/strong><br>\u51b0\u888b\uff08wet ice\uff09\u8fd0\u8f93\uff1b-20\u2103\u5e72\u71e5\u4fdd\u5b58\uff0c\u6709\u6548\u671f 12 \u4e2a\u6708\u3002<br><br><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><br>1. \u4f7f\u7528\u524d\u4f4e\u901f\u79bb\u5fc3\uff0c\u786e\u4fdd\u7c89\u672b\u5145\u5206\u6c89\u964d\u5230\u7ba1\u5e95\u540e\u518d\u6253\u5f00\u76d6\u53e3\uff1b<br>2. \u63a8\u8350\u7ec6\u80de\u589e\u6b96 EdU \u5b75\u80b2\u5de5\u4f5c\u6d53\u5ea6\u4e3a 1-50 \u03bcM\uff0c\u53ef\u4f7f\u7528 DMSO \u914d\u5236\u6210 10-50 mM \u50a8\u5b58\u6db2\u3002<br>3. \u63a8\u8350\u52a8\u7269\u9020\u6a21 EdU \u6ce8\u5c04\u91cf\u4e3a 5 mg\/kg\uff08EdU \u6ce8\u5c04\u91cf\u4e0e\u52a8\u7269\u4f53\u91cd\u7684\u6bd4\u503c\uff09\uff0c\u5b9e\u9645\u6ce8\u5c04\u91cf\u548c\u6807\u8bb0\u65f6\u95f4\uff0c\u6839\u636e\u7814\u7a76\u5185\u5bb9\u548c\u52a8\u7269\u60c5\u51b5\u800c\u5b9a\uff0c\u4f7f\u7528 PBS \u6216\u751f\u7406\u76d0\u6c34\u914d\u5236\u6210\u7ec8\u6d53\u5ea6\u4e3a 0.5-1 mg\/mL\u3002<br><br><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><br>1. \u8be5\u54c1\u5bf9\u4eba\u4f53\u6709\u5bb3\uff0c\u5e94\u907f\u514d\u5438\u5165\u672c\u54c1\u7684\u7c89\u5c18\u3002<br>2. \u672c\u4ea7\u54c1\u5efa\u8bae\u4e0e EdU \u67d3\u8272\u8bd5\u5242\u76d2\u7cfb\u5217\uff08KHI0021\u3001HKI0022\u3001HKI0023\uff09\u914d\u5957\u4f7f\u7528\u3002<br>3. \u4e3a\u4e86\u60a8\u7684\u5065\u5eb7\u548c\u5b89\u5168\uff0c\u64cd\u4f5c\u65f6\u8bf7\u7a7f\u597d\u5b9e\u9a8c\u670d\u3001\u6234\u597d\u624b\u5957\u3002<br><br><strong>\u3010\u4ea7\u54c1\u53c2\u6570\u3011<\/strong><br><strong><br><\/strong><\/figcaption><\/figure>\n\n\n\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" width=\"818\" height=\"405\" src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20240914103100809.png?imageView2\/0\/format\/webp\/q\/75\" alt=\"\" class=\"wp-image-9218\" srcset=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20240914103100809.png?imageView2\/0\/format\/webp\/q\/75 818w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20240914103100809-300x149.png?imageView2\/0\/format\/webp\/q\/75 300w\" sizes=\"(max-width: 818px) 100vw, 818px\" \/><\/figure>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-2ed40925-83a9-49bf-bdbf-21a8a61bb232\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20240914110043372.pdf\">EDU HKI0024-100mg \u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20240914110043372.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-2ed40925-83a9-49bf-bdbf-21a8a61bb232\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":7870,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[191,267,98],"tags":[],"class_list":["post-9213","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-cpzh","category-hkygrl","category-products"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:5:\"\u00a5650\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u89c4\u683c\";s:4:\"text\";s:5:\"100mg\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:2;a:5:{s:5:\"title\";s:6:\"\u8d27\u53f7\";s:4:\"text\";s:7:\"HKI0024\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:3;a:5:{s:5:\"title\";s:6:\"\u5355\u4f4d\";s:4:\"text\";s:3:\"\u652f\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";b:0;}i:4;a:5:{s:5:\"title\";s:6:\"\u54c1\u724c\";s:4:\"text\";s:8:\"HaoKebio\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}}"],"catce":["sidebar-widgets4"],"views":["1384"],"_dp_original":["9194"],"_edit_lock":["1730951409:2"],"_edit_last":["2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/9213"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=9213"}],"version-history":[{"count":6,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/9213\/revisions"}],"predecessor-version":[{"id":10587,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/9213\/revisions\/10587"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=9213"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=9213"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=9213"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}