{"id":8916,"date":"2024-09-10T10:24:51","date_gmt":"2024-09-10T02:24:51","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=8916"},"modified":"2024-09-23T14:51:43","modified_gmt":"2024-09-23T06:51:43","slug":"rna%e6%8f%90%e5%8f%96%e9%80%86%e8%bd%ac%e5%bd%95","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/8916.html","title":{"rendered":"RNA\u63d0\u53d6+\u9006\u8f6c\u5f55"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"RNA\u63d0\u53d6+\u9006\u8f6c\u5f55\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">RNA\u63d0\u53d6+\u9006\u8f6c\u5f55\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/hkfzsw\/hkrna\/\">RNA<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/hkfzsw\/\">\u5206\u5b50\u751f\u7269<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/\">\u5b9e\u9a8c\u670d\u52a1<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe560<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u6837<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKF5011<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\"><\/span><span style=\"color:#666666;\"><\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">\u6d69\u514b<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p><strong>\u670d\u52a1\u4ecb\u7ecd\uff1a<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u8d27\u53f7<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u540d\u79f0<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u89c4\u683c<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ef7\u683c<\/strong><\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">HKF5011<\/td><td class=\"has-text-align-center\" data-align=\"center\">RNA\u63d0\u53d6+\u9006\u8f6c\u5f55<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u6837<\/td><td class=\"has-text-align-center\" data-align=\"center\">60<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br>\u7ec6\u80de\u4e2d\u7684RNA\u53ef\u5206\u4e3a\u4fe1\u4f7fRNA\u3001\u8f6c\u8fd0RNA\u548c\u6838\u7cd6\u4f53RNA\u4e09\u5927\u7c7b\uff0c\u4e0d\u540c\u7ec4\u7ec7\u603bRNA\u63d0\u53d6\u5b9e\u8d28\u5c31\u662f\u5c06\u7ec6\u80de\u88c2\u89e3\uff0c\u91ca\u653e\u51faRNA\uff0c\u5e76\u901a\u8fc7\u4e0d\u540c\u65b9\u5f0f\u53bb\u9664\u86cb\u767d\u3001DNA\u7b49\u6742\u8d28\uff0c\u6700\u7ec8\u83b7\u5f97\u9ad8\u7eafRNA\u4ea7\u7269\u7684\u8fc7\u7a0b\u3002\u518d\u5728\u9006\u8f6c\u5f55\u9176\u7684\u50ac\u5316\u4e0b\uff0c\u968f\u673a\u5f15\u7269\u3001oligo(dT)\u6216\u57fa\u56e0\u7279\u5f02\u6027\u5f15\u7269\u7684\u5f15\u5bfc\u4e0b\u5408\u6210\u4e92\u8865\u7684DNA (complementary DNA\uff0ccDNA)\u3002<br><strong>\u5b9e\u9a8c\u6d41\u7a0b\uff1a<\/strong><br>\u53d6\u6750-RNA\u63d0\u53d6-\u6d4b\u6d53\u5ea6-\u9006\u8f6c\u5f55\u6210cDNA\u00a0<br>\u5176\u4e2dmRNA\u3001lncRNA\u3001circRNA\u4e3a\u666e\u901a\u9006\u8f6c\u5f55\uff1b<br>miRNA\u91c7\u53d6\u7279\u5f02\u6027\u9006\u8f6c\u5f55(\u9700\u63d0\u4f9b\u57fa\u56e0\u540d\u79f0\u548c\u79cd\u5c5e\uff0c\u65b9\u4fbf\u8bbe\u8ba1\u5f15\u7269\u7528\u4e8e\u7279\u5f02\u6027\u9006\u8f6c\u5f55 )\uff0c\u9ed8\u8ba4\u505a\u830e\u73af\u6cd5\u7684\u9006\u8f6c\u5f55\uff0c\u5982\u9700\u505a\u52a0\u5c3e\u6cd5\u9006\u8f6c\u5f55\u8bf7\u7279\u522b\u5907\u6ce8\u8bf4\u660e\u3002<\/figcaption><\/figure>\n\n\n\n<p><strong>\u9001\u6837\u8fd0\u8f93\u8981\u6c42\uff1a<\/strong><\/p>\n\n\n\n<p><strong>1.&nbsp;\u6837\u54c1\u5904\u7406<\/strong><\/p>\n\n\n\n<p><strong>a)&nbsp;\u52a8\u7269\u7ec4\u7ec7<\/strong>\uff1a\u5e38\u89c4\u7ec4\u7ec7100mg\uff0c\u8102\u80aa\u7ec4\u7ec7\uff0c\u7ed3\u7f14\u7ec4\u7ec7\uff0c\u7ea4\u7ef4\u5316\u7ec4\u7ec7\u9002\u5f53\u589e\u52a0\u3002\u5c06\u7ec4\u7ec7\u526a\u5207\u6210\u5408\u9002\u5927\u5c0f\u540e\uff08\u5efa\u8bae\u7ec4\u7ec7\u5757\u957f\u5bbd\u9ad8\u5747\u4e0d\u5927\u4e8e0.5 cm\uff09\uff0c\u7136\u540e\u8fc5\u901f\u5b8c\u5168\u6d78\u6ce1\u4e8e5\uff5e10\u500d\u4f53\u79ef\u7684RNAsolid\u2122\u8bd5\u5242\u4e2d\u3002\uff08\u6ce8\u610f\uff1a\u5df2\u6d78\u5165RNAsolid<sup>TM<\/sup>\u8bd5\u5242\u4e2d\u7684\u7ec4\u7ec7\u7ecf\u5e73\u8861\u4e00\u6bb5\u65f6\u95f4\u540e\uff0c\u53ef\u4ece\u6eb6\u6db2\u4e2d\u53d6\u51fa\uff0c\u5207\u6210\u66f4\u5c0f\u7684\u7ec4\u7ec7\u5757\uff0c\u518d\u91cd\u65b0\u653e\u56deRNAsolid<sup>TM<\/sup>\u8bd5\u5242\u4e2d\uff1b\u6709\u4e9b\u6bd4\u8f83\u5f31\u7684\u6db2\u4f53\u6e17\u900f\u6027\u7ec4\u7ec7\u5982\u9aa8\u5934\u7b49\uff0c\u4e0d\u9002\u5408\u7528RNAsolid<sup>TM<\/sup>\u8bd5\u5242\u8fdb\u884cRNA\u4fdd\u62a4\u3002\uff09<\/p>\n\n\n\n<p><strong>b)&nbsp;\u690d\u7269\u7ec4\u7ec7<\/strong>\uff1a\u65b0\u9c9c\u7684\u53f6\u3001\u679c\u8089\u3001\u79cd\u5b50\u6700\u5c11100 mg\u3002\u5c06\u5ae9\u53f6\uff0c\u5ae9\u830e\u7ec4\u7ec7\u5207\u6210\u5408\u9002\u7684\u5927\u5c0f\uff08\u5efa\u8bae\u957f\u5bbd\u9ad8\u5747\u4e0d\u5927\u4e8e0.5 cm\uff09\u540e\uff0c\u7136\u540e\u8fc5\u901f\u5b8c\u5168\u6d78\u6ce1\u4e8e5\uff5e10\u500d\u4f53\u79ef\u7684RNAsolid<sup>TM<\/sup>\u8bd5\u5242\u4e2d\u3002\uff08\u6ce8\u610f\uff1a\u67d0\u4e9b\u690d\u7269\u7ec4\u7ec7\u5929\u751f\u5177\u6709\u7684\u5c4f\u969c\uff0c\u5982\u53f6\u5b50\u8868\u9762\u7684\u8721\u5c42\u53ef\u963b\u788dRNAsolid<sup>TM<\/sup>\u8bd5\u5242\u7684\u6e17\u900f\uff0c\u5bf9\u4e8e\u6b64\u7c7b\u7ec4\u7ec7\uff0c\u901a\u5e38\u9700\u7834\u574f\u8fd9\u4e9b\u5c4f\u969c\u5c42\u4ee5\u5141\u8bb8\u6eb6\u6db2\u7684\u6d78\u900f\u3002\uff09<\/p>\n\n\n\n<p><strong>c)&nbsp;\u7ec6\u80de\u7b49\u6837\u672c<\/strong>\uff1a\u6536\u96c6\u4e0d\u5c11\u4e8e10^6\u4e2a\u7ec6\u80de\u6c89\u6dc0\uff08\u7528PBS\u6e05\u6d171-2\u6b21\uff09\uff0c\u5e72\u51b0\u8fd0\u8f93\u3002<\/p>\n\n\n\n<p><strong>d)&nbsp;\u9175\u6bcd\u3001\u7ec6\u83cc\u7b49\u6837\u672c<\/strong>\uff1a\u79bb\u5fc3\u5f03\u4e0a\u6e05\uff0c\u6536\u96c6\u4e00\u5b9a\u91cf\u7684\u83cc\u4f53\u6c89\u6dc0\uff0c\u5e72\u51b0\u8fd0\u8f93\u3002<\/p>\n\n\n\n<p><strong>e)\u5168\u8840<\/strong>\uff1a\u6700\u5c111 mL\u65b0\u9c9c\u8840\u6db2EDTA\u6297\u51dd\u7ba1\uff0c\u5e72\u51b0\u8fd0\u8f93\u3002<\/p>\n\n\n\n<p><strong>f)\u8840\u6e05<\/strong><strong>\u6216\u8840\u6d46<\/strong>\uff1a\u6700\u5c111 mL\uff0c\u5e72\u51b0\u8fd0\u8f93\u3002<\/p>\n\n\n\n<p><strong>g)<\/strong><strong>RNA\u6837\u54c1<\/strong>\uff1a OD260\/280\u4e3a1.8-2.0\uff0c\u6d53\u5ea6\u2265100 ng\/\u03bcL\uff0c\u4f53\u79ef\u226520\u03bcL\uff0c\u5e72\u51b0\u8fd0\u8f93\u3002<\/p>\n\n\n\n<p><strong>2.&nbsp;\u6837\u54c1\u4fdd\u5b58\u53ca\u8fd0\u8f93<\/strong><\/p>\n\n\n\n<p>\u52a0\u5165\u6709RNAsolid<sup>TM<\/sup>\u8bd5\u5242\u7684\u6837\u672c\u53ef\u572837\u2103\u4fdd\u5b581\uff5e2\u5929\uff0c2\u5929\u4ee5\u540e\u90e8\u5206\u7ec4\u7ec7\u4e2d\u7684RNA\u4f1a\u5f00\u59cb\u964d\u89e3\u3002\u5ba4\u6e29\uff0825\u2103\uff09\u7a33\u5b9a\u4fdd\u5b581\u5468\uff0c\u4e0d\u4f1a\u6709\u660e\u663e\u7684RNA\u964d\u89e3\u53d1\u751f\u3002\u5927\u90e8\u5206\u6837\u54c1\u7f6e\u4e8eRNAsolid<sup>TM<\/sup>\u8bd5\u5242\u4e2d\uff0c4\u2103\u6761\u4ef6\u4e0b\u53ef\u7a33\u5b9a\u4fdd\u5b581\u4e2a\u6708\uff0c\u4e0d\u4f1a\u6709\u660e\u663e\u7684RNA\u964d\u89e3\u53d1\u751f\u3002\u957f\u671f\u5b58\u653e\u53ef\u5148\u5c06\u4fdd\u5b58\u5728RNAsolid<sup>TM<\/sup>\u8bd5\u5242\u4e2d\u7684\u6837\u672c4\u2103\u6d78\u900f\u8fc7\u591c\uff0c\u7136\u540e\u5c06RNAsolid<sup>TM<\/sup>\u8bd5\u5242\u5438\u51fa\u5f03\u53bb\uff0c\u518d\u5c06\u6837\u672c\u653e\u5165-20\u2103\u6216-80\u2103\u957f\u671f\u7a33\u5b9a\u4fdd\u5b583\uff5e5\u5e74\u3002<\/p>\n\n\n\n<p>\u4ec5\u4f9b\u79d1\u7814\u7528\u9014\uff0c\u4e0d\u53ef\u7528\u4e8e\u4e34\u5e8a\u8bca\u65ad\uff01<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p>\u65e0<\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div 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