{"id":8792,"date":"2024-09-07T10:17:00","date_gmt":"2024-09-07T02:17:00","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=8792"},"modified":"2024-12-02T09:36:40","modified_gmt":"2024-12-02T01:36:40","slug":"cck8","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/8792.html","title":{"rendered":"CCK8"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"CCK8\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">CCK8\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/\">\u5b9e\u9a8c\u670d\u52a1<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/hkxbsy\/xbgnxsy\/\">\u7ec6\u80de\u529f\u80fd\u6027\u5b9e\u9a8c<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/hkxbsy\/\">\u7ec6\u80de\u5b9e\u9a8c<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe5550<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u677f<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKF6004<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\"><\/span><span style=\"color:#666666;\"><\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">\u6d69\u514b<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p><strong>\u670d\u52a1\u4ecb\u7ecd\uff1a<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u8d27\u53f7<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u540d\u79f0<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u89c4\u683c<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ef7\u683c<\/strong><\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">HKF6004<\/td><td class=\"has-text-align-center\" data-align=\"center\">CCK8<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u677f<\/td><td class=\"has-text-align-center\" data-align=\"center\">550\u5143<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>\u64cd\u4f5c\u6b65\u9aa4<\/strong><\/p>\n\n\n\n<p><strong>1. \u5236\u4f5c\u6807\u51c6\u66f2\u7ebf\uff08\u6d4b\u5b9a\u7ec6\u80de\u5177\u4f53\u6570\u91cf\u65f6\u8fdb\u884c\u6b21\u64cd\u4f5c\uff09<\/strong><\/p>\n\n\n\n<p>A)&nbsp;\u5148\u7528\u7ec6\u80de\u8ba1\u6570\u677f\u8ba1\u6570\u6240\u5236\u5907\u7684\u7ec6\u80de\u60ac\u6db2\u4e2d\u7684\u7ec6\u80de\u6570\u91cf\uff0c\u7136\u540e\u63a5\u79cd\u7ec6\u80de\u3002<br>B) \u6309\u6bd4\u4f8b\u4f9d\u6b21\u7528\u57f9\u517b\u57fa\u7b49\u6bd4\u7a00\u91ca\u6210\u4e00\u4e2a\u7ec6\u80de\u6d53\u5ea6\u68af\u5ea6\uff0c\u4e00\u822c\u8981\u505a 3-5 \u4e2a\u7ec6\u80de\u6d53\u5ea6\u68af\u5ea6\uff0c\u6bcf\u7ec4 3-6 \u4e2a\u590d\u5b54\u3002<br>C) \u63a5\u79cd\u540e\u57f9\u517b\u81f3\u7ec6\u80de\u8d34\u58c1\uff0c\u7136\u540e\u52a0 CCK-8 \u8bd5\u5242\u57f9\u517b\u4e00\u5b9a\u65f6\u95f4\u540e\u6d4b\u5b9a OD \u503c\uff0c\u5236\u4f5c\u51fa\u4e00\u6761\u4ee5\u7ec6\u80de\u6570\u91cf\u4e3a\u6a2a\u5750\u6807\uff08X\u8f74\uff09\uff0cOD \u503c\u4e3a\u7eb5\u5750\u6807\uff08Y\u8f74\uff09\u7684\u6807\u51c6\u66f2\u7ebf\u3002\u6839\u636e\u6b64\u6807\u51c6\u66f2\u7ebf\u53ef\u4ee5\u6d4b\u5b9a\u51fa\u672a\u77e5\u6837\u54c1\u7684\u7ec6\u80de\u6570\u91cf\uff08\u8bd5\u7528\u6b64\u6807\u51c6\u66f2\u7ebf\u7684\u524d\u63d0\u662f\u5b9e\u9a8c\u7684\u6761\u4ef6\u8981\u4e00\u81f4\uff0c\u4fbf\u4e8e\u786e\u5b9a\u7ec6\u80de\u7684\u63a5\u79cd\u6570\u91cf\u4ee5\u53ca\u52a0\u5165 CCK-8 \u540e\u7684\u57f9\u517b\u65f6\u95f4\uff09\u3002<\/p>\n\n\n\n<p><strong>2.&nbsp;<\/strong><strong>\u7ec6\u80de\u6d3b\u6027\u68c0\u6d4b<\/strong><\/p>\n\n\n\n<p>A) \u5728 96\u5b54\u677f\u4e2d\u63a5\u79cd\u7ec6\u80de\u60ac\u6db2\uff08100&nbsp;\u00b5L\/\u5b54\uff09\u3002\u5c06\u57f9\u517b\u677f\u653e\u5728\u57f9\u517b\u7bb1\u4e2d\u9884\u57f9\u517b\uff08\u5728 37\u2103\uff0c5% CO<sub>2<\/sub>&nbsp;\u7684\u6761\u4ef6\u4e0b\uff09\u3002<\/p>\n\n\n\n<p>B) \u5411\u6bcf\u5b54\u52a0\u5165 10&nbsp;\u00b5L CCK-8 \u6eb6\u6db2\uff08\u6ce8\u610f\u4e0d\u8981\u5728\u5b54\u4e2d\u751f\u6210\u6c14\u6ce1\uff0c\u5b83\u4eec\u4f1a\u5f71\u54cd OD \u503c\u7684\u8bfb\u6570\uff09\u3002<\/p>\n\n\n\n<p>C) \u5c06\u57f9\u517b\u677f\u5728\u57f9\u517b\u7bb1\u5185\u5b75\u80b2 2\u5c0f\u65f6\u3002<\/p>\n\n\n\n<p>D) \u7528\u9176\u6807\u4eea\u6d4b\u5b9a\u5728 450&nbsp;nm \u5904\u7684\u5438\u5149\u5ea6\u3002<\/p>\n\n\n\n<p>E) \u5982\u679c\u6682\u65f6\u4e0d\u6d4b\u5b9a OD \u503c\uff0c\u6253\u7b97\u4ee5\u540e\u6d4b\u5b9a\u7684\u8bdd\uff0c\u53ef\u4ee5\u5411\u6bcf\u5b54\u4e2d\u52a0\u5165 10&nbsp;\u00b5L 0.1M \u7684 HCl \u6216\u8005 1%SDS(W\/V)\u6eb6\u6db2\uff0c\u5e76\u906e\u76d6\u57f9\u517b\u677f\u907f\u5149\u4fdd\u5b58\u5728\u5ba4\u6e29\u6761\u4ef6\u4e0b\u3002\u5728 24&nbsp;h\u5185\u5438\u5149\u5ea6\u4e0d\u4f1a\u53d1\u751f\u53d8\u5316\u3002<\/p>\n\n\n\n<p><strong>3.&nbsp;<\/strong><strong>\u7ec6\u80de\u589e\u6b96-\u6bd2\u6027\u68c0\u6d4b<\/strong><\/p>\n\n\n\n<p>A) \u5728 96 \u5b54\u677f\u4e2d\u914d\u7f6e 100 \u00b5L \u7684\u7ec6\u80de\u60ac\u6db2\u3002\u5c06\u57f9\u517b\u677f\u5728\u57f9\u517b\u7bb1\u9884\u57f9\u517b 24 \u5c0f\u65f6\uff08\u5728 37 \u2103\uff0c5% CO2 \u7684\u6761\u4ef6\u4e0b\uff09\u3002<\/p>\n\n\n\n<p>B) \u5411\u57f9\u517b\u677f\u52a0\u5165 10&nbsp;\u00b5L \u4e0d\u540c\u6d53\u5ea6\u7684\u5f85\u6d4b\u7269\u8d28\u3002\u5728\u57f9\u517b\u7bb1\u5b75\u80b2\u4e00\u6bb5\u9002\u5f53\u7684\u65f6\u95f4\uff08\u4f8b\u5982\uff1a6\u300112\u300124 \u6216 48 \u5c0f\u65f6\uff09\u3002<\/p>\n\n\n\n<p>C) \u5411\u6bcf\u5b54\u52a0\u5165 10 \u00b5L CCK-8 \u6eb6\u6db2\uff08\u6ce8\u610f\u4e0d\u8981\u5728\u5b54\u4e2d\u751f\u6210\u6c14\u6ce1\uff0c\u5b83\u4eec\u4f1a\u5f71\u54cd OD \u503c\u7684\u8bfb\u6570\uff09\u3002\u5982\u679c\u5f85\u6d4b\u7269\u8d28\u6709\u6c27\u5316\u6027\u6216\u8fd8\u539f\u6027\u7684\u8bdd\uff0c\u53ef\u5728\u52a0 CCK-8 \u4e4b\u524d\u66f4\u6362\u65b0\u9c9c\u57f9\u517b\u57fa\uff08\u9664\u53bb\u57f9\u517b\u57fa\uff0c\u5e76\u7528\u57f9\u517b\u57fa\u6d17\u6da4\u7ec6\u80de\u4e24\u6b21\uff0c\u7136\u540e\u52a0\u5165\u65b0\u7684\u57f9\u517b\u57fa\uff09\uff0c\u53bb\u6389\u836f\u7269\u5f71\u54cd\u3002<\/p>\n\n\n\n<p>D) \u5c06\u57f9\u517b\u677f\u5728\u57f9\u517b\u7bb1\u5185\u5b75\u80b2 2\u5c0f\u65f6\u3002<\/p>\n\n\n\n<p>E) \u7528\u9176\u6807\u4eea\u6d4b\u5b9a\u5728 450 nm \u5904\u7684\u5438\u5149\u5ea6\u3002<\/p>\n\n\n\n<p>F) \u5982\u679c\u6682\u65f6\u4e0d\u6d4b\u5b9a OD \u503c\uff0c\u6253\u7b97\u4ee5\u540e\u6d4b\u5b9a\u7684\u8bdd\uff0c\u53ef\u4ee5\u5411\u6bcf\u5b54\u4e2d\u52a0\u5165 10&nbsp;\u00b5L 0.1M \u7684 HCl \u6216\u8005 1%&nbsp;SDS&nbsp;(W\/V)\u6eb6\u6db2\uff0c\u5e76\u906e\u76d6\u57f9\u517b\u677f\u907f\u5149\u4fdd\u5b58\u5728\u5ba4\u6e29\u6761\u4ef6\u4e0b\u3002\u5728 24 \u5c0f\u65f6\u5185\u5438\u5149\u5ea6\u4e0d\u4f1a\u53d1\u751f\u53d8\u5316\u3002<\/p>\n\n\n\n<p><strong>4.&nbsp;<\/strong><strong>\u6d3b\u529b\u8ba1\u7b97\uff1a.<\/strong><\/p>\n\n\n\n<p>\u7ec6\u80de\u6d3b\u529b\uff08\uff05\uff09=[A(\u52a0\u836f)\uff0dA(\u7a7a\u767d)]\uff0f[ A(0 \u52a0\u836f)\uff0dA(\u7a7a\u767d)]\u00d7100<\/p>\n\n\n\n<p>A\uff08\u52a0\u836f\uff09\uff1a\u5177\u6709\u7ec6\u80de\u3001CCK-8 \u6eb6\u6db2\u548c\u836f\u7269\u6eb6\u6db2\u7684\u5b54\u7684\u5438\u5149\u5ea6<\/p>\n\n\n\n<p>A\uff08\u7a7a\u767d\uff09\uff1a\u5177\u6709\u57f9\u517b\u57fa\u548c CCK-8 \u6eb6\u6db2\u800c\u6ca1\u6709\u7ec6\u80de\u7684\u5b54\u7684\u5438\u5149\u5ea6<\/p>\n\n\n\n<p>A\uff080 \u52a0\u836f\uff09\uff1a\u5177\u6709\u7ec6\u80de\u3001CCK-8 \u6eb6\u6db2\u800c\u6ca1\u6709\u836f\u7269\u6eb6\u6db2\u7684\u5b54\u7684\u5438\u5149\u5ea6<\/p>\n\n\n\n<p>\u7ec6\u80de\u6d3b\u529b\uff1a\u7ec6\u80de\u589e\u6b96\u6d3b\u529b\u6216\u7ec6\u80de\u6bd2\u6027\u6d3b\u529b<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p>\u65e0<\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div 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