{"id":862,"date":"2019-12-07T22:34:35","date_gmt":"2019-12-07T14:33:45","guid":{"rendered":"http:\/\/haokebio.com\/862.html"},"modified":"2019-12-07T22:33:45","modified_gmt":"2019-12-07T14:33:45","slug":"ab4ceb053b","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/862.html","title":{"rendered":"\u6d41\u5f0fROS\u68c0\u6d4b"},"content":{"rendered":"
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\u3000\u3000\u4e00\u3001\u5b9e\u9a8c\u539f\u7406<\/strong><\/p>\n

 <\/p>\n

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\u3000\u3000DCFH-DA\u672c\u8eab\u6ca1\u6709\u8367\u5149\uff0c\u53ef\u4ee5\u81ea\u7531\u7a7f\u8fc7\u7ec6\u80de\u819c\uff0c\u8fdb\u5165\u7ec6\u80de\u540e\uff0c\u88ab\u6c34\u89e3\u751f\u6210DCFH\u3002\u800cDCFH\u4e0d\u80fd\u900f\u8fc7\u7ec6\u80de\u819c\u3002\u7ec6\u80de\u5185\u7684ROS\u53ef\u4ee5\u6c27\u5316\u65e0\u8367\u5149\u7684DCFH\u751f\u6210\u6709\u8367\u5149\u7684DCF\u3002DCF\u88ab488nm\u6fc0\u53d1\u5149\u6fc0\u53d1\u540e\uff0c\u53d1\u5c04530nm\u7684\u8367\u5149\u4fe1\u53f7\uff0c\u88abFITC\u901a\u9053\u63a5\u6536\uff0c\u68c0\u6d4bDCF\u7684\u8367\u5149\u5c31\u53ef\u4ee5\u5224\u5b9a\u7ec6\u80de\u5185\u6d3b\u6027\u6c27ROS\u7684\u6c34\u5e73\u3002<\/p>\n

\u3000\u3000\u4e8c\u3001\u5b9e\u9a8c\u6b65\u9aa4<\/strong><\/p>\n

 <\/p>\n

\u3000\u30001\u3001\u6536\u96c6\u7ec6\u80de\uff1a\u7ec6\u80de\u60ac\u6db2\u8f6c\u5165\u79bb\u5fc3\u7ba1\u4e2d\uff0c1500 rpm\u79bb\u5fc35 min\u6536\u96c6\u7ec6\u80de,\u5f03\u4e0a\u6e05\u3002<\/p>\n

\u3000\u30002\u3001PBS\u6d17\u6da4\u7ec6\u80de\uff1a\u52a0\u51653ml 4\u2103\u9884\u51b7\u7684PBS\u5b8c\u5168\u91cd\u60ac\u7ec6\u80de\uff0c1500 rpm\u79bb\u5fc35 min\uff0c\u5f03\u4e0a\u6e05\u3002\u6c89\u6dc0\u9707\u8361\u6df7\u5300\u3002<\/p>\n

\u3000\u30003\u3001\u7ec6\u80de\u8ba1\u6570\uff1a\u79fb\u53d610 ul\u5728\u8840\u7ec6\u80de\u8ba1\u6570\u4eea\u4e0a\u8fdb\u884c\u7ec6\u80de\u8ba1\u6570,\u8c03\u6574\u7ec6\u80de\u4e3a1×10^6-2×10^8\/ml\u3002<\/p>\n

\u3000\u30004\u3001\u88c5\u8f7d\u63a2\u9488\uff1a\u6309\u71671:1000\u7528\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u7a00\u91caDCFH-DA\uff0c\u4f7f\u7ec8\u6d53\u5ea6\u4e3a10umol\/L\u3002<\/p>\n

\u3000\u30005\u3001\u5b75\u80b2\u63a2\u9488\uff1a37\u2103\u7ec6\u80de\u57f9\u517b\u7bb1\u5185\u5b75\u80b220 min\uff0c\u6bcf\u96943-5 min\u98a0\u5012\u6df7\u5300\u4e00\u4e0b\uff0c\u4f7f\u63a2\u9488\u548c\u7ec6\u80de\u5145\u5206\u63a5\u89e6\uff0c\u7528\u65e0\u8840\u6e05\u7684\u57f9\u517b\u6db2\u6d17\u6da4\u7ec6\u80de\u4e09\u6b21\uff0c\u4ee5\u53bb\u9664\u672a\u8fdb\u5165\u7ec6\u80de\u5185\u7684\u63a2\u9488\u3002<\/p>\n

\u3000\u30006\u3001\u4e0a\u673a\u68c0\u6d4b\uff1a\u6d41\u5f0f\u7ec6\u80de\u4eea\u4f7f\u7528\u6fc0\u53d1\u6ce2\u957f488nm\uff0c\u53d1\u5c04\u6ce2\u957f525±20nm\u8fdb\u884c\u68c0\u6d4b\uff0c\u7528FlowJo\u8f6f\u4ef6\u5206\u6790\u7ec6\u80deROS\u6c34\u5e73\u3002<\/p>\n

\u3000\u3000\u4e09\u3001\u7ed3\u679c\u5206\u6790<\/strong><\/p>\n

 <\/p>\n

\"\u6d41\u5f0fROS\u68c0\u6d4b\"
 <\/p>\n

\n\n\n\n\n\n\n
\u6837\u672c\u7f16\u53f7<\/td>\n\u5e73\u5747\u8367\u5149\u5f3a\u5ea6<\/td>\n<\/tr>\n
THP-1-nc.fcs<\/td>\n1395<\/td>\n<\/tr>\n
THP-1-rosup.fcs<\/td>\n6221<\/td>\n<\/tr>\n
THP-1.fcs<\/td>\n18397<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

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\u3000\u3000\u4e00\u3001\u5b9e\u9a8c\u539f\u7406   \u3000\u3000\u6d3b\u6027\u6c27\u7c07ROS\u662f\u7531\u6c27\u5206\u5b50\u8fd8\u539f\u6240\u5f62\u6210\u7684\u5206\u5b50\u6216\u8005\u79bb\u5b50\uff0c\u5927\u90e8\u5206\u90fd\u662f\u7531\u7ebf\u7c92\u4f53\u4ea7\u751f\u7684 […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-862","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["3267"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/862"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=862"}],"version-history":[{"count":0,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/862\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=862"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=862"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=862"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}