{"id":8587,"date":"2024-09-05T11:38:50","date_gmt":"2024-09-05T03:38:50","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=8587"},"modified":"2024-09-23T15:10:00","modified_gmt":"2024-09-23T07:10:00","slug":"%e5%ae%9a%e5%88%b6%e5%8e%9f%e4%bd%8d%e6%9d%82%e4%ba%a4%e6%8e%a2%e9%92%88","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/8587.html","title":{"rendered":"\u5b9a\u5236\u539f\u4f4d\u6742\u4ea4\u63a2\u9488"},"content":{"rendered":"<div class=\"bpp-post-content\">\n<div class=\"am-article-bd am-page-mm am-cf\">\n<p>\u3000\u3000<strong>\u9879\u76ee\u4ecb\u7ecd<\/strong><\/p>\n<p>\u539f\u4f4d\u6742\u4ea4\u6280\u672f(In situ hybridization\uff0cISH)\u662f\u5229\u7528\u6838\u9178\u5206\u5b50\u4e92\u8865\u914d\u5bf9\u4f7f\u6742\u4ea4\u63a2\u9488\u53ef\u4ee5\u7279\u5f02\u6027\u7ed3\u5408\u4e8e\u7ec4\u7ec7\u6216\u7ec6\u80de\u4e2d\u76ee\u6807\u6838\u9178\u5206\u5b50\uff0c\u518d\u901a\u8fc7\u63a2\u9488\u5e26\u6709\u7684\u5730\u9ad8\u8f9b\u6216\u8005\u8367\u5149\u6807\u8bb0\u7269\uff0c\u7279\u5f02\u6027\u9274\u5b9a\u3001\u68c0\u6d4b\u76ee\u6807\u6838\u9178\u5206\u5b50\u7684\u76f8\u5bf9\u542b\u91cf\u4ee5\u53ca\u5728\u7ec4\u7ec7\u7ec6\u80de\u4e2d\u7684\u5b9a\u4f4d\u3002\u56e0\u6b64\u539f\u4f4d\u6742\u4ea4\u53ef\u4ee5\u5728\u7ec4\u7ec7\u6216\u7ec6\u80de\u539f\u6709\u7ed3\u6784\u57fa\u7840\u4e4b\u4e0a(\u7ec4\u7ec7\u5207\u7247\u6216\u7ec6\u80de)\uff0c\u68c0\u6d4b\u67d0\u79cd\u57fa\u56e0\u7684\u6709\u65e0\uff0c\u5b9a\u4f4d\u53ca\u534a\u5b9a\u91cf\u3002\u8367\u5149\u6807\u8bb0\u63a2\u9488\u8fdb\u884c\u539f\u4f4d\u6742\u4ea4\u5373\u4e3aFISH(Fluorescence in situ hybridization)\uff0c\u53ef\u4ee5\u66ff\u4ee3\u653e\u5c04\u6807\u8bb0\u7269\uff0c\u7075\u654f\u5ea6\u9ad8\u3001\u5b9e\u9a8c\u6548\u7387\u9ad8\u3002<\/p>\n<p><strong>\u5e94\u7528<\/strong><\/p>\n<p>\u539f\u4f4d\u6742\u4ea4\u53ef\u4ee5\u5b9a\u6027\u68c0\u6d4b\u7ec4\u7ec7\u7ec6\u80de\u4e2d\u7684mRNA\u3001lncRNA\u3001circRNA\uff0c\u4e5f\u53ef\u4ee5\u5b9a\u4f4d\u53ca\u5206\u6790\u67d3\u8272\u8d28\u4e2d\u7684\u57fa\u56e0\u3002\u5229\u7528\u7279\u5f02\u602716s RNA\u63a2\u9488\u8fd8\u53ef\u4ee5\u7b80\u5355\u6837\u54c1\u4e2d\u7684\u7ec6\u83cc\u3001\u53e4\u7ec6\u83cc\uff0c\u8fd8\u53ef\u4ee5\u901a\u8fc7\u7279\u610f\u63a2\u9488\u9274\u5b9a\u7ec4\u7ec7\u7ec6\u80de\u4e2d\u7684\u75c5\u6bd2\u3002FISH\u80fd\u591f\u7528\u6765\u68c0\u6d4b\u6216\u786e\u8ba4\u57fa\u56e0\u6216\u67d3\u8272\u4f53\u662f\u5426\u5f02\u5e38\uff0c\u533b\u5b66\u548c\u54c1\u79cd\u9274\u5b9a\u3001DNA\u7279\u5b9a\u529f\u80fd\u7814\u7a76\u3002\u5229\u7528FISH\u65b9\u6cd5\u8fd8\u53ef\u68c0\u6d4b\u975e\u6574\u500d\u4f53\u3001\u57fa\u56e0\u91cd\u6392\u3001\u62f7\u8d1d\u6570\u53d8\u5f02\u7b49\u3002<\/p>\n<p><strong>\u6837\u672c\u4fdd\u5b58\u8fd0\u8f93<\/strong><\/p>\n<p><strong>\u7ec4\u7ec7\u51b0\u51bb\u5207\u7247<\/strong><\/p>\n<p>\u7ec4\u7ec7\u5207\u53d6\u9700\u8981\u90e8\u4f4d\uff0c\u539a\u5ea6\u57282mm \u5de6\u53f3, PBS \u6f02\u6d17\uff0c\u5e72\u51b0\u8fd0\u8f93\u6216\u7acb\u5373\u7f6e\u4e8e\u56fa\u5b9a\u6db2\u4e2d\u3002\u7ec4\u7ec7\u56fa\u5b9a\u6709\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u52a8\u7269)\u3001\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u690d\u7269)\u3001\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u8f83\u5ae9\u690d\u7269)\uff0c\u5ba4\u6e29\u56fa\u5b9a12 \u5c0f\u65f6(\u6839\u636e\u7ec4\u7ec7\u5927\u5c0f)\u4ee5\u4e0a\uff0c\u690d\u7269\u7ec4\u7ec7\u56fa\u5b9a\u65f6\u9700\u5e38\u6e29\u62bd\u771f\u7a7a30min\uff0c\u518d\u7528\u7eaf\u6c34\u6216PBS \u6e05\u6d17\u4e24\u6b21\u540e\uff0c \u653e\u516515% \u8517\u7cd6\u6eb6\u6db2\u4e2d\uff0c4\u2103\u8131\u6c348 \u5c0f\u540e\uff0c\u636230% \u8517\u7cd6\u6eb6\u6db24\u2103\u8fc7\u591c\u3002\u51b0\u5207\u65f6\u4f69\u6234\u624b\u5957\u3002\u51b0\u51bb\u5207\u7247\u653e-20\u2103\u6216-80\u2103 \u4fdd\u5b58\u5907\u7528\u3002<\/p>\n<p><strong>\u7ec4\u7ec7\u77f3\u8721\u5207\u7247<\/strong><\/p>\n<p>\u7ec4\u7ec7\u5207\u53d6\u9700\u8981\u90e8\u4f4d\uff0c\u539a\u5ea6\u57282mm \u5de6\u53f3, PBS \u6f02\u6d17\u540e\u7acb\u5373\u56fa\u5b9a\u3002\u52a8\u7269\u7ec4\u7ec7\u7528\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u52a8\u7269)\uff0c\u690d\u7269\u7ec4\u7ec7\u7528\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u690d\u7269)\u6216\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u8f83\u5ae9\u690d\u7269)\uff0c\u690d\u7269\u7ec4\u7ec7\u56fa\u5b9a\u65f6\u9700\u5e38\u6e29\u62bd\u771f\u7a7a30min\uff0c\u56fa\u5b9a12h \u4ee5\u4e0a\u3002\u56fa\u5b9a\u5b8c\u6210\u540e\u8131\u6c34\u3001\u5305\u57cb\u3001\u5207\u7247\u3002\u68af\u5ea6\u9152\u7cbe\u914d\u5236\u9700\u7528DEPC \u6c34\u3002\u77f3\u8721\u5e38\u89c4\u4fdd\u5b58\u5907\u7528\u3002<\/p>\n<p><strong>\u6807\u672c\u8fd0\u8f93<\/strong><\/p>\n<p>\u51b0\u51bb\u5207\u7247\uff1a\u6807\u672c\u653e\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u4e2d\uff0c\u5e38\u6e29\u8fd0\u8f93;\u51b0\u51bb\u5207\u7247-20\u2103\u8fd0\u8f93;\u65b0\u9c9c\u7ec4\u7ec7\u5e72\u51b0\u8fd0\u8f93\u3002\u4ea4\u63a5\u5355\u968f\u6837\u672c\u4e00\u8d77\uff0c\u5355\u72ec\u5c01\u88c5\u3002<\/p>\n<p>\u77f3\u8721\u5207\u7247\uff1a\u6807\u672c\u653e\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u4e2d\uff0c\u5e38\u6e29\u8fd0\u8f93;\u77f3\u8721\u5207\u7247\u5e38\u6e29\u8fd0\u8f93\u3002\u4ea4\u63a5\u5355\u968f\u6837\u672c\u4e00\u8d77\uff0c\u5355\u72ec\u5c01\u88c5\u3002<\/p>\n<p>\u7ec6\u80de\u722c\u7247\uff1a\u7ec6\u80de\u722c\u7247\u52a0\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u540e\u5bc6\u5c014\u2103\u8fd0\u8f93\u3002\u4ea4\u63a5\u5355\u968f\u6837\u672c\u4e00\u8d77\uff0c\u5355\u72ec\u5c01\u88c5\u3002<\/p>\n<p><strong>DEPC \u6c34\u914d\u5236<\/strong><\/p>\n<p>\u7eaf\u6c34\u4e2d\u52a0\u51650.1%DEPC \u539f\u6db2\u9707\u8361\u6405\u62cc2h \u540e\u9759\u7f6e\u8fc7\u591c\u540e\u9ad8\u538b\u706d\u83cc\uff0c\u6709\u6548\u706d\u83cc\u65f6\u95f430min \u4ee5\u4e0a\uff0c\u6216\u76f4\u63a5\u8d2d\u4e70\u3002<\/p>\n<p><strong>\u7ed3\u679c\u5c55\u793a<\/strong><\/p>\n<p><img decoding=\"async\" class=\"alignnone size-full wp-image-2314\" src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806352962.jpg?imageView2\/0\/format\/webp\/q\/75\" alt=\"\" width=\"1440\" height=\"1024\" srcset=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806352962.jpg?imageView2\/0\/format\/webp\/q\/75 1440w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806352962-150x107.jpg?imageView2\/0\/format\/webp\/q\/75 150w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806352962-300x213.jpg?imageView2\/0\/format\/webp\/q\/75 300w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806352962-1024x728.jpg?imageView2\/0\/format\/webp\/q\/75 1024w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806352962-768x546.jpg?imageView2\/0\/format\/webp\/q\/75 768w\" sizes=\"(max-width: 1440px) 100vw, 1440px\" \/><\/p>\n<p><img decoding=\"async\" class=\"alignnone size-full wp-image-2315\" src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806354220.jpg?imageView2\/0\/format\/webp\/q\/75\" alt=\"\" width=\"1440\" height=\"1024\" srcset=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806354220.jpg?imageView2\/0\/format\/webp\/q\/75 1440w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806354220-150x107.jpg?imageView2\/0\/format\/webp\/q\/75 150w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806354220-300x213.jpg?imageView2\/0\/format\/webp\/q\/75 300w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806354220-1024x728.jpg?imageView2\/0\/format\/webp\/q\/75 1024w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806354220-768x546.jpg?imageView2\/0\/format\/webp\/q\/75 768w\" sizes=\"(max-width: 1440px) 100vw, 1440px\" \/><\/p>\n<p><img decoding=\"async\" class=\"alignnone size-medium wp-image-2316\" src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806355222.jpg?imageView2\/0\/format\/webp\/q\/75\" alt=\"\" width=\"1440\" height=\"1024\" srcset=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806355222.jpg?imageView2\/0\/format\/webp\/q\/75 1440w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806355222-150x107.jpg?imageView2\/0\/format\/webp\/q\/75 150w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806355222-300x213.jpg?imageView2\/0\/format\/webp\/q\/75 300w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806355222-1024x728.jpg?imageView2\/0\/format\/webp\/q\/75 1024w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806355222-768x546.jpg?imageView2\/0\/format\/webp\/q\/75 768w\" sizes=\"(max-width: 1440px) 100vw, 1440px\" \/><\/p>\n<p><img decoding=\"async\" class=\"alignnone size-medium wp-image-2317\" src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806360431.jpg?imageView2\/0\/format\/webp\/q\/75\" alt=\"\" width=\"1440\" height=\"1024\" srcset=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806360431.jpg?imageView2\/0\/format\/webp\/q\/75 1440w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806360431-150x107.jpg?imageView2\/0\/format\/webp\/q\/75 150w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806360431-300x213.jpg?imageView2\/0\/format\/webp\/q\/75 300w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806360431-1024x728.jpg?imageView2\/0\/format\/webp\/q\/75 1024w, https:\/\/www.haokebio.com\/wp-content\/uploads\/2019\/12\/2021120806360431-768x546.jpg?imageView2\/0\/format\/webp\/q\/75 768w\" sizes=\"(max-width: 1440px) 100vw, 1440px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u00a0<\/strong><\/p>\n<p>\u3000\u3000<strong>\u4ef7\u683c\u5217\u8868<\/strong><\/p>\n<ul>\n<li>\n<table class=\"am-table am-table-bordered am-table-radius am-table-striped am-table-hover am-text-sm\" style=\"width: 598px; height: 501px;\" border=\"1\" cellspacing=\"0\" align=\"center\">\n<tbody>\n<tr>\n<td style=\"width: 139px;\"><strong>\u670d\u52a1\u9879\u76ee<\/strong><\/td>\n<td style=\"width: 79px;\"><strong>\u4ef7\u683c<\/strong><\/td>\n<\/tr>\n<tr>\n<td style=\"width: 139px;\">Tunel\uff08\u767d\u5149\uff09\u68c0\u6d4b<\/td>\n<td style=\"width: 79px;\">200\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 139px;\">Tunel\uff08\u8367\u5149\uff09\u68c0\u6d4b<\/td>\n<td style=\"width: 79px;\">200\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 139px;\">IF Tunel \u53cc\u67d3<\/td>\n<td style=\"width: 79px;\">250\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 139px;\">Tunel( \u82af\u7247\uff09<\/td>\n<td style=\"width: 79px;\">550\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 139px;\">\u539f\u4f4d\u6742\u4ea4\uff08DAB \u663e\u8272\uff09<\/td>\n<td style=\"width: 79px;\">240\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 139px;\">\u539f\u4f4d\u6742\u4ea4\uff08FISH \u5355\u6807\uff09<\/td>\n<td style=\"width: 79px;\">240\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 152px;\">\u539f\u4f4d\u6742\u4ea4\uff08FISH\u3001IF \u53cc\u67d3\uff09<\/td>\n<td style=\"width: 66px;\">300\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 152px;\">\u539f\u4f4d\u6742\u4ea4\uff08FISH \u53cc\u6807\uff09<\/td>\n<td style=\"width: 66px;\">300\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 152px;\">\u539f\u4f4d\u6742\u4ea4\uff08FISH \u4e09\u6807\uff09<\/td>\n<td style=\"width: 66px;\">350\u5143\/\u5f20<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 152px;\">\u539f\u4f4d\u6742\u4ea4\uff08\u82af\u7247\uff09<\/td>\n<td style=\"width: 66px;\">500 \u5143\u8d77<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 152px;\">\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001BIO\uff09<\/td>\n<td style=\"width: 66px;\">600\u5143\/\u6761<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 152px;\">\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001DIG\uff09<\/td>\n<td style=\"width: 66px;\">1000 \u5143 \/ \u6761<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 153px;\">\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001FAM\uff09<\/td>\n<td style=\"width: 73px;\">1100 \u5143 \/ \u6761<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 153px;\">\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001Cy3\uff09<\/td>\n<td style=\"width: 73px;\">1900 \u5143 \/ \u6761<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 153px;\">\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001BIO\uff09<\/td>\n<td style=\"width: 73px;\">1200 \u5143 \/ \u6761<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 153px;\">\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001DIG\uff09<\/td>\n<td style=\"width: 73px;\">1690 \u5143 \/ \u6761<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 153px;\">\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001FAM\uff09<\/td>\n<td style=\"width: 73px;\">1400 \u5143 \/ \u6761<\/td>\n<\/tr>\n<tr>\n<td style=\"width: 153px;\">\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001Cy3\uff09<\/td>\n<td style=\"width: 73px;\">2500 \u5143 \/ \u6761<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/li>\n<\/ul>\n<div style=\"clear: both;\"><\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u3000\u9879\u76ee\u4ecb\u7ecd \u539f\u4f4d\u6742\u4ea4\u6280\u672f(In situ hybridization\uff0cISH)\u662f\u5229\u7528\u6838\u9178\u5206\u5b50\u4e92\u8865\u914d\u5bf9\u4f7f\u6742\u4ea4\u63a2 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