{"id":839,"date":"2019-12-07T22:33:31","date_gmt":"2019-12-07T14:32:41","guid":{"rendered":"http:\/\/haokebio.com\/839.html"},"modified":"2019-12-07T22:32:41","modified_gmt":"2019-12-07T14:32:41","slug":"533e210a49","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/839.html","title":{"rendered":"RNA(si RNA,m i RNA,sh RNA)\u8ba2\u8d2d"},"content":{"rendered":"
\n
\n

\u3000\u3000\u7b80\u4ecb\uff1a<\/strong><\/p>\n

\u3000\u3000RNA\u5e72\u6d89(RNAinterference\uff0cRNAi)\u662f\u6709\u6548\u6c89\u9ed8\u6216\u6291\u5236\u76ee\u6807\u57fa\u56e0\u8868\u8fbe\u7684\u8fc7\u7a0b\uff0c\u6307\u5185\u6e90\u6027\u6216\u5916\u6e90\u6027\u53cc\u94feRNA(dsRNA)\u4ecb\u5bfc\u7684\u7ec6\u80de\u5185mRNA\u53d1\u751f\u7279\u5f02\u6027\u964d\u89e3\uff0c\u4ece\u800c\u5bfc\u81f4\u9776\u57fa\u56e0\u7684\u8868\u8fbe\u6c89\u9ed8\uff0c\u4ea7\u751f\u76f8\u5e94\u7684\u529f\u80fd\u8868\u578b\u7f3a\u5931\u7684\u73b0\u8c61\u3002RNA\u5e72\u6270\u7531\u8f6c\u8fd0\u5230\u7ec6\u80de\u7ec6\u80de\u8d28\u4e2d\u7684\u53cc\u94feRNA\u6fc0\u6d3b\u3002\u6c89\u9ed8\u673a\u5236\u53ef\u5bfc\u81f4\u7531\u5c0f\u5e72\u6270RNA(siRNA)\u6216\u77ed\u53d1\u5939RNA(shRNA)\u8bf1\u5bfc\u5b9e\u73b0\u9776mRNA\u7684\u964d\u89e3\uff0c\u6216\u8005\u901a\u8fc7\u5c0fRNA(miRNA)\u8bf1\u5bfc\u7279\u5b9amRNA\u7ffb\u8bd1\u7684\u6291\u5236\u3002\u901a\u8fc7\u77ed\u53cd\u4e49\u6838\u9178(siRNA\u548cshRNA\u5e8f\u5217)\u9501\u5b9a\u7ec6\u80demRNA\uff0c\u4ece\u800c\u5b9e\u73b0\u5176\u968f\u540e\u7684\u964d\u89e3\u3002\u8fd9\u53cd\u8fc7\u6765\u963b\u65ad\u4e86\u8be5\u86cb\u767d\u7684\u8fdb\u4e00\u6b65\u8868\u8fbe\/\u805a\u96c6\uff0c\u5bfc\u81f4\u5176\u6c34\u5e73\u7684\u4e0b\u964d\uff0c\u6700\u7ec8\u5b9e\u73b0\u6291\u5236\u4f5c\u7528<\/p>\n

\n\n\n\n\n\n\n
 <\/td>\n\u957f\u5ea6<\/strong><\/td>\n\u63cf\u8ff0<\/strong><\/td>\n<\/tr>\n
siRNA<\/td>\n19-29\u4e2a\u6838\u82f7\u9178<\/td>\n\u5c0f\u5e72\u6270RNA\uff0c\u57283'\u7aef\u6709\u4e24\u4e2a\u78b1\u57fa\u7684\u6e38\u79bb\uff0c\u53ef\u6fc0\u6d3bRNA\u5e72\u6270\uff0c\u901a\u8fc7\u4e0e\u76ee\u6807mRNA\u7279\u5f02\u6027\u5730\u4e92\u8865\u7ed3\u5408\u5b9e\u73b0mRNA\u964d\u89e3\u3002<\/td>\n<\/tr>\n
shRNA<\/td>\n\u8d28\u7c92<\/td>\n\u77ed\u53d1\u5939RNA\uff0c\u5305\u542b\u4e00\u4e2a\u73af\u7ed3\u6784\uff0c\u53ef\u52a0\u5de5\u6210siRNA\uff0c\u4e5f\u53ef\u901a\u8fc7\u4e0e\u76ee\u6807mRNA\u7279\u5f02\u6027\u5730\u4e92\u8865\u7ed3\u5408\u5b9e\u73b0mRNA\u964d\u89e3\u3002<\/td>\n<\/tr>\n
miRNA<\/td>\n20-25\u4e2a\u6838\u82f7\u9178<\/td>\n\u7531\u8f83\u957f\u7684\u521d\u7ea7\u8f6c\u5f55\u7269\u7ecf\u8fc7\u4e00\u7cfb\u5217\u6838\u9178\u9176\u7684\u526a\u5207\u52a0\u5de5\u800c\u4ea7\u751f\u7684\uff0c\u968f\u540e\u7ec4\u88c5\u8fdbRNA\u8bf1\u5bfc\u7684\u6c89\u9ed8\u590d\u5408\u4f53\uff0c\u901a\u8fc7\u78b1\u57fa\u4e92\u8865\u914d\u5bf9\u7684\u65b9\u5f0f\u8bc6\u522b\u9776mRNA\uff0c\u5e76\u6839\u636e\u4e92\u8865\u7a0b\u5ea6\u7684\u4e0d\u540c\u6307\u5bfc\u6c89\u9ed8\u590d\u5408\u4f53\u964d\u89e3\u9776mRNA\u6216\u8005\u963b\u904f\u9776mRNA\u7684\u7ffb\u8bd1\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u3000\u3000\u516c\u53f8\u53ef\u4ee5\u6839\u636e\u7528\u6237\u8981\u6c42\u63d0\u4f9b\u9ad8\u8d28\u91cf\u7684\u5316\u5b66\u5408\u6210siRNA\/miRNA\uff0c\u6784\u5efashRNA\u3002<\/strong>
 <\/p>\n

\u3000\u3000\u5ba2\u6237\u63d0\u4f9b\u4fe1\u606f\uff1a<\/strong><\/p>\n

\u3000\u30001.\u76ee\u7684\u57fa\u56e0\u7684\u8be6\u7ec6\u4fe1\u606f(\u79cd\u5c5e\uff0c\u57fa\u56e0ID\uff0cNM\u53f7\u7b49);<\/p>\n

\u3000\u30002.\u5b9e\u9a8c\u9700\u6c42(\u662f\u5426\u643a\u5e26\u7279\u6b8a\u6807\u7b7e)<\/p>\n

\u3000\u3000\u4ea4\u4ed8\u7ed3\u679c\uff1a<\/strong><\/p>\n

\u3000\u30001.\u5408\u6210\u62a5\u544a<\/p>\n

\u3000\u30002.siRNA\/shRNA\/miRNA\u8bd5\u5242<\/p>\n

\u3000\u3000\u5b9e\u9a8c\u62a5\u4ef7\uff1a<\/strong><\/p>\n

\u3000\u30001\uff0cmiRNA\u76f8\u5173\u8ba2\u8d2d<\/strong><\/p>\n

\u3000\u3000\u4eba\u548c\u9f20\u7684\uff0cMimics\u53caNC\uff0c1500\u5143\uff0c5nmole(2OD\uff0c1\u7ba1\u88c5)<\/p>\n

\u3000\u3000\u4eba\u548c\u9f20\u7684\uff0cinhibitor\u53caNC\uff0c1500\u5143\uff0c5nmole(2OD\uff0c1\u7ba1\u88c5)<\/p>\n

\u3000\u3000\u5176\u4ed6\u79cd\u5c5e\u9700\u8981\u8be2\u4ef7\u3002<\/p>\n

\u3000\u30002\uff0csiRNA\u5e72\u6270\/\u6c89\u9ed8\/\u6572\u4f4e\/\u6572\u51cf<\/strong><\/p>\n

\u3000\u3000\u5355\u6761\u5e8f\u5217\u5408\u6210\uff1a<\/strong> 600\u5143\/\u6761\uff0c\u5468\u671f1-2\u5468\uff0c\u5ba2\u6237\u63d0\u4f9b\u8bbe\u8ba1\u597d\u7684\u5e8f\u5217\u3002<\/p>\n

\u3000\u30003\u4fdd1\u5957\u9910\uff1a<\/strong>2300\u5143\uff0c\u5468\u671f1-2\u5468<\/p>\n

\u3000\u3000(\u5e38\u89c4\u8bbe\u8ba13\u6761\uff0c\u60a8\u7b5b\u9009\u5982\u679c\u90fd\u6ca1\u6709\u6548\u679c\u7684\u8bdd\uff0c\u53ef\u4ee5\u514d\u8d39\u5e2e\u60a8\u518d\u8bbe\u8ba1\u5408\u6210\u4e00\u6b21)<\/p>\n

\u3000\u30004\u4fdd1\u5957\u9910\uff1a<\/strong>3000\u5143 \uff0c\u5468\u671f1-2\u5468<\/p>\n

\u3000\u3000\u5907\u6ce8 \uff1a\u5ba2\u6237\u63d0\u4f9b\u8be6\u7ec6\u57fa\u56e0\u4fe1\u606f(\u79cd\u5c5e\uff0c\u57fa\u56e0\u540d\u79f0\uff0c\u57fa\u56e0ID\u7b49)\u3002<\/p>\n

\u3000\u3000\u4e00\u822c150bp\u5de6\u53f3\u80fd\u8bbe\u8ba1\u51fa\u4e00\u6761si\uff0c\u5957\u9910\u81f3\u5c11\u9700\u89816\u6761\u624d\u80fd\u505a\u5957\u9910<\/p>\n

\u3000\u3000\u5ba2\u6237\u5f97\u5230\uff1a<\/strong>\u7535\u5b50\u7248\u7684siRNA\u5e8f\u5217\uff0c2\u4e2aOD\u7684siRNA\u51bb\u5e72\u7c89\uff0cNC\u5bf9\u7167\u90011\u4e2aOD<\/p>\n

\u3000\u30003\uff0cshRNA\u5e72\u6270\/\u6c89\u9ed8\/\u6572\u4f4e\/\u6572\u51cf<\/strong><\/p>\n

\u3000\u3000\u5355\u6761\u5e8f\u5217\uff1a<\/strong> 900\u5143\/\u6761\uff0c\u5468\u671f2-3\u5468<\/p>\n

\u3000\u30004\u4fdd1\u5957\u9910\uff1a<\/strong>3500\u5143\uff0c\u5468\u671f2-3\u5468<\/p>\n

\u3000\u3000\u5907\u6ce8 \uff1a\u5ba2\u6237\u63d0\u4f9b\u8be6\u7ec6\u57fa\u56e0\u4fe1\u606f(\u79cd\u5c5e\uff0c\u57fa\u56e0\u540d\u79f0\uff0c\u57fa\u56e0ID\u7b49)\u3002\u9700\u8981\u8d28\u7c92\u63d0\u53d6\u53e6\u6536500\u5143\/\u6761\u3002\u4e00\u822c150bp\u5de6\u53f3\u80fd\u8bbe\u8ba1\u51fa\u4e00\u6761sh\uff0c\u5957\u9910\u81f3\u5c11\u9700\u89818\u6761\u624d\u80fd\u505a\u5957\u9910<\/p>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u3000\u3000\u7b80\u4ecb\uff1a \u3000\u3000RNA\u5e72\u6d89(RNAinterference\uff0cRNAi)\u662f\u6709\u6548\u6c89\u9ed8\u6216\u6291\u5236\u76ee\u6807\u57fa\u56e0\u8868\u8fbe\u7684\u8fc7\u7a0b\uff0c\u6307\u5185 […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-839","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["1285"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/839"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=839"}],"version-history":[{"count":0,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/839\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=839"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=839"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=839"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}