{"id":836,"date":"2019-12-07T22:33:05","date_gmt":"2019-12-07T14:32:15","guid":{"rendered":"http:\/\/haokebio.com\/836.html"},"modified":"2019-12-07T22:32:15","modified_gmt":"2019-12-07T14:32:15","slug":"ce4098c7d6","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/836.html","title":{"rendered":"\u7ec6\u80de\u8f6c\u67d3"},"content":{"rendered":"
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\u3000\u3000\u4e00\u3001\u5b9e\u9a8c\u7b80\u4ecb<\/strong><\/p>\n

\u3000\u3000\u7ec6\u80de\u8f6c\u67d3\u662f\u6307\u5c06\u5916\u6e90\u5206\u5b50\u5982DNA\u3001RNA\u7b49\u5bfc\u5165\u771f\u6838\u7ec6\u80de\u7684\u6280\u672f\u3002\u4e3b\u8981\u5305\u62ec\uff1a\u7535\u7a7f\u5b54\u6cd5\u3001\u663e\u5fae\u6ce8\u5c04\u3001\u57fa\u56e0\u67aa\u3001\u78f7\u9178\u9499\u5171\u6c89\u6dc0\u6cd5\u3001\u8102\u8d28\u4f53\u8f6c\u67d3\u6cd5\u3001\u75c5\u6bd2\u4ecb\u5bfc\u7684\u8f6c\u67d3\u7b49\uff0c\u7406\u60f3\u7684\u8f6c\u67d3\u65b9\u6cd5\u662f\u5177\u6709\u9ad8\u8f6c\u67d3\u6548\u7387\u3001\u5bf9\u7ec6\u80de\u6bd2\u6027\u4f5c\u7528\u5c0f\u7b49\u3002\u5176\u4e2d\u8102\u8d28\u4f53\u8f6c\u67d3\u662f\u5e38\u7528\u7684\u7b80\u4fbf\u8f6c\u67d3\u65b9\u6cd5\u3002<\/p>\n

\u3000\u3000\u8102\u8d28\u4f53(liposome)\u8f6c\u67d3\u65b9\u6cd5\u7684\u539f\u7406\u5728\u4e8e\uff0c\u9633\u79bb\u5b50\u8102\u8d28\u4f53\u8868\u9762\u5e26\u6b63\u7535\u8377\uff0c\u80fd\u4e0e\u6838\u9178\u7684\u78f7\u9178\u6839\u901a\u8fc7\u9759\u7535\u4f5c\u7528\u5c06DNA\u5206\u5b50\u5305\u88f9\uff0c\u5f62\u6210DNA-\u8102\u590d\u5408\u4f53\uff0c\u88ab\u8868\u9762\u5e26\u8d1f\u7535\u8377\u7684\u7ec6\u80de\u819c\u5438\u9644\u3002\u518d\u901a\u8fc7\u819c\u7684\u878d\u5408\u6216\u7ec6\u80de\u7684\u5185\u541e\u4f5c\u7528\uff0c\u5076\u5c14\u901a\u8fc7\u76f4\u63a5\u6e17\u900f\u4f5c\u7528\uff0c\u5c06DNA\u8f6c\u8fd0\u81f3\u7ec6\u80de\u5185\uff0c\u5f62\u6210\u5305\u6db5\u4f53\u6216\u8fdb\u5165\u6eb6\u9176\u4f53\u3002\u5f53DNA\u4ece\u5305\u6db5\u4f53\u5185\u91ca\u653e\u540e\uff0c\u8fdb\u5165\u7ec6\u80de\u8d28\uff0c\u518d\u8fdb\u4e00\u6b65\u8fdb\u5165\u6838\u5185\u5b9e\u73b0\u8f6c\u5f55\u3001\u8868\u8fbe\u3002<\/p>\n

\u3000\u3000\u4e8c\u3001siRNA\/shRNA\/miRNA\/DNA\u8f6c\u67d3\u7ec6\u80de\u5b9e\u9a8c\u6b65\u9aa4(\u4ee56\u5b54\u677f\u4e3a\u4f8b)<\/strong><\/p>\n

\u3000\u30001 \u8f6c\u67d3\u524d\u4e00\u5929\u63a5\u79cd\u7ec6\u80de\u4e8e6\u5b54\u677f\uff0c5×104\u6bcf\u5b54\uff0c\u7b2c\u4e8c\u5929\u8f6c\u67d3\u65f6\u7ec6\u80de\u6c47\u5408\u5ea6\u8fbe\u523060%-70%\u6bcf\u5b54\u3002<\/p>\n

\u3000\u30002 \u8f6c\u67d3\u524d\u534a\u5c0f\u65f6\u5c06\u5b8c\u5168\u57f9\u517b\u57fa\u6362\u65e0\u8840\u6e05\u57f9\u517b\u57fa1.9 ml\u3002<\/p>\n

\u3000\u30003 A\u6db2\uff1aRNA100pmol\/DNA 2μg(\u6839\u636e\u6838\u9178\u7c7b\u578b\u4e0d\u540c\uff0c\u7528\u91cf\u4e0d\u540c)\u52a0\u516550 ul Opti-MEM\u65e0\u8840\u6e05\u57f9\u517b\u57fa\uff0c\u8f7b\u8f7b\u6df7\u5300\uff0c\u5ba4\u6e29\u9759\u7f6e5min\u3002<\/p>\n

\u3000\u30004 B\u6db2\uff1a\u8102\u8d28\u4f53\u4f7f\u7528\u524d\u8f7b\u8f7b\u6df7\u5300\uff0c\u8102\u8d28\u4f535ul \u52a0\u516550 ul Opti-MEM\u65e0\u8840\u6e05\u57f9\u517b\u57fa\uff0c\u8f7b\u8f7b\u6df7\u5300\uff0c\u5ba4\u6e29\u9759\u7f6e5min\u3002<\/p>\n

\u3000\u30005 B\u6db2\u52a0\u5165\u5230A\u6db2\u4e2d\uff0c\u7528\u67aa\u8f7b\u8f7b\u6df7\u5300\uff0c\u5ba4\u6e29\u9759\u6b6220min\u3002<\/p>\n

\u3000\u30006 \u5c06\u6df7\u5408\u6db2\u5747\u5300\u5206\u6563\u6162\u6162\u6ef4\u52a0\u52306\u5b54\u677f\u7ec6\u80de\u4e2d\uff0c\u8fb9\u6ef4\u52a0\u8fb9\u524d\u540e\u5de6\u53f3\u5341\u5b57\u4ea4\u53c9\u8f7b\u8f7b\u6447\u6643\u3002<\/p>\n

\u3000\u30007 \u5b75\u7bb137\u2103\u57f9\u517b4~6h\uff0c\u7136\u540e\u5c06\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u6362\u6210\u5b8c\u5168\u57f9\u517b\u57fa\u7ee7\u7eed\u57f9\u517b\u3002<\/p>\n

\u3000\u30008 mRNA \u6c34\u5e73\u7684\u68c0\u6d4b\uff1a\u5728\u8f6c\u67d3\u540e24-48h\u540e\uff0c\u7528Real-time PCR\u7684\u65b9\u6cd5\u5728mRNA\u6c34\u5e73\u8fdb\u884c\u68c0\u6d4b\u3002<\/p>\n

\u3000\u30009 \u86cb\u767d\u6c34\u5e73\u7684\u68c0\u6d4b\uff1a\u5728\u8f6c\u67d3\u540e48-72h\u540e\uff0c\u7528Western-blot\u6216\u514d\u75ab\u7ec4\u5316\u7684\u65b9\u6cd5\u5728\u86cb\u767d\u6c34\u5e73\u8fdb\u884c\u68c0\u6d4b\u3002<\/p>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u3000\u3000\u4e00\u3001\u5b9e\u9a8c\u7b80\u4ecb \u3000\u3000\u7ec6\u80de\u8f6c\u67d3\u662f\u6307\u5c06\u5916\u6e90\u5206\u5b50\u5982DNA\u3001RNA\u7b49\u5bfc\u5165\u771f\u6838\u7ec6\u80de\u7684\u6280\u672f\u3002\u4e3b\u8981\u5305\u62ec\uff1a\u7535\u7a7f\u5b54\u6cd5\u3001\u663e\u5fae\u6ce8\u5c04 […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-836","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["879"],"_edit_lock":["1727321429:2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/836"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=836"}],"version-history":[{"count":0,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/836\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=836"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=836"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=836"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}