{"id":8236,"date":"2024-09-02T11:36:51","date_gmt":"2024-09-02T03:36:51","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=8236"},"modified":"2024-10-10T09:28:55","modified_gmt":"2024-10-10T01:28:55","slug":"%e4%b8%80%e6%ad%a5%e6%b3%95tunel%e7%bb%86%e8%83%9e%e5%87%8b%e4%ba%a1%e6%a3%80%e6%b5%8b%e8%af%95%e5%89%82%e7%9b%92%ef%bc%88%e7%ba%a2%ef%bc%8caf555","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/8236.html","title":{"rendered":"\u4e00\u6b65\u6cd5TUNEL\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555)"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u4e00\u6b65\u6cd5tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555HKI0011-1.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u4e00\u6b65\u6cd5tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555HKI0011-1.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"\u4e00\u6b65\u6cd5TUNEL\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555)\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">\u4e00\u6b65\u6cd5TUNEL\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555)<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/cpzh\/zhother\/\">\u5176\u4ed6<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/cpzh\/\">\u5206\u5b50\u75c5\u7406<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a51500<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">50T<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKI0011<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u74f6<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\">\n<figure style=\"font-size:15px\" class=\"wp-block-table\"><table><tbody><tr><td><strong>\u8d27\u53f7<\/strong><\/td><td><strong>\u54c1\u540d<\/strong><\/td><td><strong>50T<\/strong><\/td><td><strong>100T<\/strong><\/td><\/tr><tr><td>HKI0011-1<\/td><td>AF555\u8367\u5149\u53cd\u5e94\u6db2<\/td><td>2.5ml<\/td><td>5ml<\/td><\/tr><tr><td>HKI0011-2<\/td><td>TdT\u9176<\/td><td>50ul<\/td><td>100ul<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><br>\u7ec6\u80de\u5728\u53d1\u751f\u51cb\u4ea1\u65f6\uff0c\u4f1a\u6fc0\u6d3b\u4e00\u4e9b DNA \u5185\u5207\u9176\uff0c\u8fd9\u4e9b\u5185\u5207\u9176\u4f1a\u5207\u65ad\u6838\u5c0f\u4f53\u95f4\u7684\u57fa\u56e0\u7ec4 DNA\u3002&nbsp;\u7ec6\u80de\u51cb\u4ea1\u65f6\u62bd\u63d0 DNA&nbsp;\u8fdb\u884c\u7535\u6cf3\u68c0\u6d4b\uff0c\u53ef\u4ee5\u53d1\u73b0 180-200bp \u7684 DNAladder\u3002\u57fa\u56e0\u7ec4 DNA \u65ad\u88c2\u65f6\uff0c&nbsp;\u66b4\u9732\u7684 3&#8242;-OH&nbsp;\u53ef\u4ee5\u5728\u672b\u7aef\u8131\u6c27\u6838\u82f7\u9178\u8f6c\u79fb\u9176(TerminalDeoxynucleotidyl &nbsp;Transferase,TdT) \u7684\u50ac\u5316\u4e0b\u52a0\u4e0a\u7ea2\u8272 AF555 \u6807\u8bb0\u7684dUTP\uff0c\u4ece\u800c\u53ef\u4ee5\u901a\u8fc7\u8367\u5149\u663e\u5fae\u955c\u6216\u6d41\u5f0f\u7ec6\u80de\u4eea\u8fdb\u884c\u68c0\u6d4b\uff0c\u8fd9\u5c31 \u662f TUNEL(TdT-mediated&nbsp;&nbsp;dUTPNick-End&nbsp;Labeling)\u6cd5\u68c0\u6d4b\u7ec6\u80de\u51cb\u4ea1\u7684\u539f\u7406<br><br><strong>\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/strong><br>\u51b0\u888b\u8fd0\u8f93\uff0c-20\u02daC&nbsp;\u50a8\u5b58\uff0c\u53ef\u7a33\u5b9a\u50a8\u5b58\u4e00\u5e74<\/figcaption><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p style=\"font-size:15px\"><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><\/p>\n\n\n\n<p style=\"font-size:15px\">\u77f3\u8721\u5207\u7247\u8367\u5149tunel\u5b9e\u9a8c\u6b65\u9aa4<\/p>\n\n\n\n<p style=\"font-size:15px\">\u77f3\u8721\u5207\u7247\u8131\u8721\u81f3\u6c34\uff1a\u4f9d\u6b21\u5c06\u5207\u7247\u653e\u5165\u4e8c\u7532\u82ef \u2160 15min-\u4e8c\u7532\u82ef\u216115min-\u65e0\u6c34\u4e59\u9187 \u21605min-\u65e0\u6c34&nbsp;\u4e59\u9187\u21615min\uff0c\u53d6\u51fa\u7f6e\u4e8e\u901a\u98ce\u53a8\u5185\u7b49\u9152\u7cbe\u667e\u5e72\u540e\u653e\u5165\u81ea\u6765\u6c34\u4e2d\u7a0d\u6d17\uff0c\u84b8\u998f\u6c34\u6d17\u3002(\u51ac\u5929\u5e94\u63d0\u9ad8\u8131\u8721 \u6e29\u5ea6\u6216\u9002\u5f53\u5ef6\u957f\u8131\u8721\u65f6\u95f4)<\/p>\n\n\n\n<p style=\"font-size:15px\">\u901a\u900f\uff1apH6.0 \u67e0\u6aac\u9178\u4fee\u590d\u6db2\u4e2d\u706b\u4fee\u590d8min \uff0c\u5ba4\u6e29\u51b7\u5374\uff0c\u7528\u514d\u75ab\u7ec4\u5316\u7b14\u5212\u5708\uff0c\u4e4b\u540e\u7eaf\u6c34\u6d17\u4e09 \u6b21(\u91cd\u8981)\u3002 &nbsp;(\u901a\u900f\u65b9\u5f0f\u6709\u591a\u949f\uff1a1&nbsp;\u86cb\u767d\u9176\u6d88\u5316\uff0c2tritonx100 \u901a\u900f\u5242\u901a\u900f\uff0c\u67e0\u6aac\u9178\u4fee\u590d)<\/p>\n\n\n\n<p style=\"font-size:15px\">\u914d\u8bd5\u5242\u5de5\u4f5c\u6db2\u4ee5\u53ca\u5b75\u80b2\uff1a\u8367\u5149\u53cd\u5e94\u6db2\u548c TdT \u9176\u6309 50:1 \u6bd4\u4f8b\u6df7\u5408\uff0c\u6b64\u6db2\u4e3a tunel \u5de5\u4f5c\u6db2\uff0c\uff0c&nbsp;\u5c06 tunel&nbsp;\u5de5\u4f5c\u6db2\u52a0\u5165\u5230\u5708\u5185\u8986\u76d6\u7ec4\u7ec7\uff0c\u5207\u7247\u5e73\u653e\u4e8e\u6e7f\u76d2\u5185\uff0c37\u2103\u6052\u6e29\u7bb1\u5b75\u80b2 1 \u5230 2 \u5c0f\u65f6\uff0c\u6e7f\u76d2&nbsp;\u5185\u52a0\u5c11\u91cf\u6c34\u4fdd\u6301\u6e7f\u5ea6\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">DAPI&nbsp;\u590d\u67d3\u7ec6\u80de\u6838\uff1a\u5207\u7247\u7528 PBS (PH7.4) \u6d17\u6da4 3 \u6b21\uff0c\u6bcf\u6b21 5min\u3002\u53bb\u9664 PBS \u540e\u5728\u5708\u5185\u6ef4\u52a0 DAPI&nbsp;\u67d3\u6db2\uff0c\u907f\u5149\u5ba4\u6e29\u5b75\u80b2 10min\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">\u5c01\u7247\uff1a\u73bb\u7247\u7f6e\u4e8e PBS (PH7.4) \u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da4 3 \u6b21\uff0c\u6bcf\u6b215min\u3002\u5207\u7247\u7a0d\u7529\u5e72\u540e\u7528&nbsp;\u6297\u8367\u5149\u6dec\u706d\u5c01\u7247\u5242\u5c01\u7247\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">\u955c\u68c0\u62cd\u7167\uff1a\u5207\u7247\u4e8e\u5c3c\u5eb7\u5012\u7f6e\u8367\u5149\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u5e76\u91c7\u96c6\u56fe\u50cf\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p style=\"font-size:15px\"><strong>\u77f3\u8721\u5207\u7247\u8367\u5149tunel\u7ed3\u679c\u5224\u8bfb<\/strong><\/p>\n\n\n\n<p style=\"font-size:15px\">DAPI&nbsp;\u67d3\u51fa\u6765\u7684\u7ec6\u80de\u6838\u5728\u7d2b\u5916\u7684\u6fc0\u53d1\u4e0b\u4e3a\u84dd\u8272\uff0c\u8bd5\u5242\u76d2\u4e3a AF555 \u6807\u8bb0\uff0c\u9633\u6027\u51cb\u4ea1\u7ec6\u80de\u6838\u4e3a\u7ea2&nbsp;\u8272\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p style=\"font-size:15px\"><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><\/p>\n\n\n\n<p style=\"font-size:15px\">1. \u672c\u8bd5\u5242\u4ec5\u4f9b\u79d1\u7814\u3002<\/p>\n\n\n\n<p style=\"font-size:15px\">2. \u64cd\u4f5c\u65f6\u8bf7\u7a7f\u5b9e\u9a8c\u670d\uff0c\u4f69\u6234\u4e00\u6b21\u6027\u624b\u5957\u3002<\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-e190de99-97ca-4fde-acee-66196cbb155a\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20241010092841464.pdf\">\u4e00\u6b65\u6cd5tunel\u51cb\u4ea1\u68c0\u67e5\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555) HKI0011  \u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/09\/20241010092841464.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-e190de99-97ca-4fde-acee-66196cbb155a\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":4834,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[195,191,98],"tags":[],"class_list":["post-8236","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-zhother","category-cpzh","category-products"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:6:\"\u00a51500\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u89c4\u683c\";s:4:\"text\";s:3:\"50T\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:2;a:5:{s:5:\"title\";s:6:\"\u8d27\u53f7\";s:4:\"text\";s:7:\"HKI0011\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:3;a:5:{s:5:\"title\";s:6:\"\u5355\u4f4d\";s:4:\"text\";s:3:\"\u74f6\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";b:0;}i:4;a:5:{s:5:\"title\";s:6:\"\u54c1\u724c\";s:4:\"text\";s:8:\"HaoKebio\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}}"],"catce":["sidebar-widgets4"],"views":["1691"],"_dp_original":["4184"],"_edit_lock":["1730691840:2"],"_edit_last":["2"],"_thumbnail_id":["4834"]},"medium_url":"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u4e00\u6b65\u6cd5tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555HKI0011-300x229.jpg","thumbnail_url":"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u4e00\u6b65\u6cd5tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555HKI0011-100x100.jpg","full_url":"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u4e00\u6b65\u6cd5tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08\u7ea2\uff0cAF555HKI0011.jpg","_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/8236"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=8236"}],"version-history":[{"count":4,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/8236\/revisions"}],"predecessor-version":[{"id":10622,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/8236\/revisions\/10622"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media\/4834"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=8236"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=8236"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=8236"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}