{"id":6085,"date":"2024-05-22T17:02:03","date_gmt":"2024-05-22T09:02:03","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=6085"},"modified":"2024-11-22T17:09:53","modified_gmt":"2024-11-22T09:09:53","slug":"ki-67%e5%85%94%e5%8d%95%e5%85%8b%e9%9a%86%e6%8a%97%e4%bd%93-2","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/6085.html","title":{"rendered":"Ki-67\u5154\u5355\u514b\u9686\u6297\u4f53"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/20241017094702389.png?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/20241017094702389.png?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"Ki-67\u5154\u5355\u514b\u9686\u6297\u4f53\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">Ki-67\u5154\u5355\u514b\u9686\u6297\u4f53<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/kt\/yk\/\">\u4e00\u6297<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/kt\/\">\u6297\u4f53<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a5600<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100ul<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKZ150327<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u652f<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><th><strong>\u54c1\u724c<\/strong><\/th><th><strong>\u5546\u54c1\u7f16\u7801<\/strong><\/th><th><strong>\u5546\u54c1\u540d\u79f0<\/strong><\/th><th><strong>\u89c4\u683c<\/strong><\/th><th><strong>\u5355\u4f4d<\/strong><\/th><th><strong>\u5355\u4ef7<\/strong><\/th><\/tr><tr><th>HaoKebio<\/th><th>HKZ150327<\/th><th>Ki-67\u5154\u5355\u514b\u9686\u6297\u4f53<\/th><th>100ul<\/th><th>\u652f<\/th><th>600<\/th><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><strong>Ki-67<\/strong><br><strong>Recombinant\u00a0Rabbit\u00a0Monoclonal Antibody\u00a0Product\u00a0Datasheet<\/strong><br><strong>Catalog# \u00a0<\/strong>BX50204\u00a0<strong>Clone# \u00a0<\/strong>BP6181<br><strong>Predicted\u00a0Molecular Wt:<\/strong><br>359kDa<br><strong>Purity: \u00a0\u00a0<\/strong>ProA\u00a0affinity\u00a0purified\u00a0IgG<br><strong>Species Cross-reactivity:<\/strong><br>Human<br><strong>Form: \u00a0\u00a0<\/strong>Liquid<br><strong>Applications:<\/strong><br>IHC-P<br><strong>Swissprot\u00a0ID: \u00a0\u00a0<\/strong>P46013<br><strong>Background:<\/strong><br>Antigen\u00a0Ki-67\u00a0is a\u00a0nuclear\u00a0protein that\u00a0is associated with and\u00a0may\u00a0be\u00a0necessary\u00a0for\u00a0cellular\u00a0proliferation.\u00a0Furthermore,\u00a0it\u00a0is\u00a0associated with\u00a0ribosomal\u00a0RNA transcription.<br>Ki-67 was\u00a0present\u00a0in the\u00a0nuclei of cells\u00a0in the G1,S,and\u00a0G2\u00a0phases\u00a0of the\u00a0cell\u00a0division\u00a0cycle\u00a0as\u00a0well\u00a0as\u00a0in\u00a0mitosis.\u00a0Quiescent\u00a0or\u00a0resting\u00a0cells\u00a0in\u00a0the\u00a0G0\u00a0phase\u00a0did\u00a0not\u00a0express\u00a0the\u00a0Ki-67\u00a0antigen.<br>Ki-67\u00a0protein was\u00a0used as diagnostic tools\u00a0in\u00a0different types\u00a0of\u00a0neoplasms\u00a0because\u00a0it was\u00a0present\u00a0in\u00a0all\u00a0proliferating\u00a0cells<br>(normal and tumor cells) and was\u00a0an\u00a0excellent\u00a0operational\u00a0marker\u00a0to\u00a0determine\u00a0the\u00a0growth\u00a0fraction\u00a0of\u00a0a\u00a0given\u00a0cell\u00a0population.<br><strong>Subcellular\u00a0location:<\/strong><br>Nucleus<br><strong>Recommended\u00a0method:<\/strong><br>Heat\u00a0induced epitope\u00a0retrieval with Tris-EDTA\u00a0buffer\u00a0\u00a0\u00a0(pH\u00a09.0),\u00a0primary\u00a0antibody\u00a0incubate\u00a0at\u00a0RT\u00a0(18\u00b0C-25\u00b0C)\u00a0for\u00a030\u00a0minutes.<br><strong>Immunogen:<\/strong><br>Synthetic\u00a0peptide corresponding to\u00a0residues within<br>aa1000 to\u00a0aa1100 of\u00a0Ki-67 was\u00a0used\u00a0as\u00a0an\u00a0immunogen.<br><strong>Storage\u00a0Buffer:<\/strong><br>PBS\u00a059%, Sodium\u00a0azide\u00a00.01%,\u00a0Glycerol\u00a040%,\u00a0BSA 0.05%.<br><strong>Storage conditions:<\/strong><br>-25\u00b0C\u00a0to\u00a0-18\u00b0C<br><strong>Storage instructions:<\/strong><br>Shipped on\u00a0blue\u00a0ice.\u00a0Upon delivery,\u00a0aliquot,\u00a0and\u00a0store\u00a0at\u00a0-25\u00b0C\u00a0to\u00a0-18\u00b0C. Avoid\u00a0freeze\u00a0\/\u00a0thaw\u00a0cycles.<br><strong>Recommended\u00a0Dilutions:<\/strong><br>IHC-P:\u00a0\u00a0\u00a0\u00a0\u00a01:100-1:200<br><strong>Background\u00a0References:<\/strong><br>1.\u00a0Scholzen\u00a0T, et.al, J\u00a0Cell\u00a0Physiol.\u00a02000\u00a0Mar;182(3):311-\u00a022.<br>2. \u00a0Inwald\u00a0EC, et.al,\u00a0Breast\u00a0Cancer\u00a0Res\u00a0Treat.\u00a02013\u00a0Jun;139(2):539-52.<br>Immunohistochemistry<br>(Formalin\/PFA-fixed<br>paraffin-embedded<br>sections)\u00a0analysis of tonsil\u00a0labelling\u00a0Ki-67\u00a0with<br>BP6181.\u00a0Heat\u00a0mediated antigen\u00a0retrieval was<br>performed\u00a0using<br>Tris\/EDTA\u00a0buffer\u00a0pH\u00a09.0.<\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-fd3bc6cf-2388-4c3e-8de2-bbf50f7537b7\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/20241122170938378.pdf\">HKZ150327_BP6181_Ki-67<\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/20241122170938378.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-fd3bc6cf-2388-4c3e-8de2-bbf50f7537b7\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":11596,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[101,99,98],"tags":[],"class_list":["post-6085","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-yk","category-kt","category-products"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"views":["832"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:5:\"\u00a5600\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u89c4\u683c\";s:4:\"text\";s:5:\"100ul\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:2;a:5:{s:5:\"title\";s:6:\"\u8d27\u53f7\";s:4:\"text\";s:9:\"HKZ150327\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:3;a:5:{s:5:\"title\";s:6:\"\u5355\u4f4d\";s:4:\"text\";s:3:\"\u652f\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";b:0;}i:4;a:5:{s:5:\"title\";s:6:\"\u54c1\u724c\";s:4:\"text\";s:8:\"HaoKebio\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}}"],"catce":["sidebar-widgets4"],"_thumbnail_id":["11596"],"_dp_original":["6083"],"_edit_lock":["1732266594:2"],"_edit_last":["2"]},"medium_url":"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/20241017094702389.png","thumbnail_url":"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/20241017094702389-251x193.png","full_url":"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/20241017094702389.png","_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/6085"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=6085"}],"version-history":[{"count":6,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/6085\/revisions"}],"predecessor-version":[{"id":12709,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/6085\/revisions\/12709"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media\/11596"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=6085"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=6085"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=6085"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}