{"id":4401,"date":"2023-07-13T15:51:03","date_gmt":"2023-07-13T07:51:03","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=4401"},"modified":"2024-11-04T11:48:27","modified_gmt":"2024-11-04T03:48:27","slug":"%e7%99%bd%e5%85%89tunel%e6%a3%80%e6%9f%a5%e8%af%95%e5%89%82%e7%9b%92%ef%bc%88dab-2","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/4401.html","title":{"rendered":"\u767d\u5149tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08DAB)"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u6d69\u514blogo-scaled.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u6d69\u514blogo-scaled.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"\u767d\u5149tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08DAB)\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">\u767d\u5149tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08DAB)<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/cpzh\/zhother\/\">\u5176\u4ed6<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/cpzh\/\">\u5206\u5b50\u75c5\u7406<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a51500<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">50T<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKI0012<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u4e2a<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p style=\"font-size:15px\"><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><\/p>\n\n\n\n<p>\u7ec6\u80de\u51cb\u4ea1\u4e2d\u67d3\u8272\u4f53 DNA \u7684\u65ad\u88c2\u662f\u4e2a\u6e10\u8fdb\u7684\u9636\u6bb5\u6027\u8fc7\u7a0b\u3002\u67d3\u8272\u4f53 DNA \u9996\u5148\u5728\u5185\u6e90\u6027\u7684\u6838\u9178\u6c34\u89e3\u9176\u7684\u4f5c\u7528\u4e0b\u964d\u89e3\u4e3a 50-300 kb \u7684\u5927\u7247\u6bb5\uff0c\u7136\u540e\u5927\u7ea6 30%\u7684\u67d3\u8272\u4f53 DNA \u5728 Ca 2+\u548c Mg 2+\u4f9d\u8d56\u7684\u6838\u9178\u5185\u5207\u9176\u4f5c\u7528\u4e0b\uff0c\u5728\u6838\u5c0f\u4f53\u5355\u4f4d\u4e4b\u95f4\u88ab\u968f\u673a\u5207\u65ad\uff0c\u5f62\u6210 180-200 bp \u6838\u5c0f\u4f53 DNA \u591a\u805a\u4f53\u3002\u56e0\u6b64\u5728\u7ec6\u80de\u51cb\u4ea1\u665a\u671f\uff0cDNA \u4f1a\u88ab\u964d\u89e3\u4e3a 180-200 bp \u7684\u7247\u6bb5\uff0c\u65ad\u88c2\u7684\u57fa\u56e0\u7ec4 DNA \u4e0a\u66b4\u9732\u51fa\u5927\u91cf\u7684 3&#8242;-OH \u672b\u7aef\u3002\u672b\u7aef\u8131\u6c27\u6838\u7cd6\u6838\u82f7\u9178\u8f6c\u79fb\u9176\uff08Terminal Deoxynucleotidyl Transferase, TdT\uff09\u662f\u4e00\u79cd\u4e0d\u4f9d\u8d56\u4e8e\u6a21\u677f\u7684 DNA \u805a\u5408\u9176\uff0c\u53ef\u4ee5\u50ac\u5316\u8131\u6c27\u6838\u82f7\u9178\u7ed3\u5408\u5230\u65ad\u88c2\u7684 DNA \u5206\u5b50 3&#8242;-OH \u672b\u7aef\u3002\u56e0\u6b64 TUNEL (TdT mediated dUTP Nick End Labeling)\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u8bd5\u5242\u76d2\u53ef\u4ee5\u7528\u6765\u68c0\u6d4b\u7ec4\u7ec7\u7ec6\u80de\u5728\u51cb\u4ea1\u665a\u671f\u8fc7\u7a0b\u4e2d\u7ec6\u80de\u6838 DNA \u7684\u65ad\u88c2\u60c5\u51b5\u3002\u5176\u539f\u7406\u662f\u5728 TdT \u9176\u7684\u4f5c\u7528\u4e0b\uff0c\u5728\u57fa\u56e0\u7ec4DNA \u65ad\u88c2\u65f6\u66b4\u9732\u51fa\u7684 3\u00b4-OH \u672b\u7aef\u63ba\u5165\u751f\u7269\u7d20\u6807\u8bb0\u7684 dUTP\uff08Biotin-dUTP\uff09\uff0c\u968f\u540e\u7528\u8fa3\u6839\u8fc7\u6c27\u5316\u7269\u9176\uff08Horse-radish peroxidase\uff0cHRP\uff09\u6807\u8bb0\u7684\u94fe\u9709\u4eb2\u548c\u7d20\uff08Streptavidin\uff09(Streptavidin-HRP,SA-HRP)\uff0c\u68c0\u6d4b\u88ab\u751f\u7269\u7d20\uff08Biotin\uff09\u6807\u8bb0\u7684 DNA \u672b\u7aef\uff0c\u6700\u540e\u901a\u8fc7\u52a0\u5165 HRP \u7684\u5e95\u7269\u6df7\u5408\u6db2\uff08DAB\uff09\u8fdb\u884c\u663e\u8272\u53cd\u5e94\uff0c\u4f7f\u5f97\u51cb\u4ea1\u7ec6\u80de\u7684\u7ec6\u80de\u6838\u88ab\u67d3\u6210\u68d5\u9ec4\u8272\uff0c\u4ece\u800c\u53ef\u4ee5\u7528\u666e\u901a\u5149\u5b66\u663e\u5fae\u955c\u68c0\u6d4b\u3002<\/p>\n\n\n\n<p>\u672c\u8bd5\u5242\u76d2\u53ef\u9002\u7528\u4e8e\u77f3\u8721\u7ec4\u7ec7\u5207\u7247\u7684\u7ec6\u80de\u51cb\u4ea1\u68c0\u6d4b\u3002<\/p>\n\n\n\n<p><strong>\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/strong><\/p>\n\n\n\n<p>\u51b0\u888b\u8fd0\u8f93\uff0c-20\u02da C \u50a8\u5b58\uff0c\u53ef\u7a33\u5b9a\u50a8\u5b58\u4e00\u5e74\u3002<\/p>\n\n\n\n<p><strong>\u3010\u8bd5\u5242\u7ec4\u6210\u3011<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>\u8d27\u53f7<\/td><td>\u54c1\u540d<\/td><td>50T<\/td><td>100T<\/td><\/tr><tr><td>HKI0012-1<\/td><td>\u767d\u5149Tunel\u53cd\u5e94\u6db2<\/td><td>2.5ml<\/td><td>5ml<\/td><\/tr><tr><td>HKI0012-2<\/td><td>TdT\u9176<\/td><td>50ul<\/td><td>100ul<\/td><\/tr><tr><td>HKI0012-3<\/td><td>Streptavidin-HRP\uff08\u8367\u5149\u65e0\uff09<\/td><td>25ul<\/td><td>50ul<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><\/p>\n\n\n\n<p><strong>\u77f3\u8721\u5207\u7247\u8367\u5149 tunel \u5b9e\u9a8c\u6b65\u9aa4<\/strong><\/p>\n\n\n\n<p><strong>\u77f3\u8721\u5207\u7247\u8131\u8721\u81f3\u6c34\uff1a<\/strong>\u4f9d\u6b21\u5c06\u5207\u7247\u653e\u5165\u4e8c\u7532\u82ef\u216015min-\u4e8c\u7532\u82ef\u216115min-\u65e0\u6c34\u4e59\u9187\u21605min-\u65e0\u6c34\u4e59\u9187\u21615min\uff0c\u53d6\u51fa\u7f6e\u4e8e\u901a\u98ce\u53a8\u5185\u7b49\u9152\u7cbe\u667e\u5e72\u540e\u653e\u5165\u81ea\u6765\u6c34\u4e2d\u7a0d\u6d17\uff0c\u84b8\u998f\u6c34\u6d17\u3002(\u51ac\u5929\u5e94\u63d0\u9ad8\u8131\u8721\u6e29\u5ea6\u6216\u9002\u5f53\u5ef6\u957f\u8131\u8721\u65f6\u95f4\uff09<\/p>\n\n\n\n<p><strong>\u901a\u900f\uff1a<\/strong>PH6.0 \u67e0\u6aac\u9178\u4fee\u590d\u6db2\u4e2d\u706b\u4fee\u590d 8min \uff0c\u5ba4\u6e29\u51b7\u5374\uff0c\u7528\u514d\u75ab\u7ec4\u5316\u7b14\u5212\u5708\uff0c\u4e4b\u540e\u7eaf\u6c34\u6d17\u4e09\u6b21(\u91cd\u8981)\u3002\uff08\u901a\u900f\u65b9\u5f0f\u6709\u591a\u79cd\uff1a1 \u86cb\u767d\u9176\u6d88\u5316\uff0c2tritonx100 \u901a\u900f\u5242\u901a\u900f\uff0c\u67e0\u6aac\u9178\u4fee\u590d\uff09<\/p>\n\n\n\n<p><strong>\u914d\u8bd5\u5242\u5de5\u4f5c\u6db2\u4ee5\u53ca\u5b75\u80b2\uff1a<\/strong>\u8367\u5149\u53cd\u5e94\u6db2\uff08\u767d\u5149 tunel \u53cd\u5e94\u6db2\uff09\u548c TdT \u9176\u6309 50:1 \u6bd4\u4f8b\u6df7\u5408\uff0c\u6b64\u6db2\u4e3a tunel \u5de5\u4f5c\u6db2\uff0c\uff0c\u5c06 tunel \u5de5\u4f5c\u6db2\u52a0\u5165\u5230\u5708\u5185\u8986\u76d6\u7ec4\u7ec7\uff0c\u5207\u7247\u5e73\u653e\u4e8e\u6e7f\u76d2\u5185\uff0c37\u2103\u6052\u6e29\u7bb1\u5b75\u80b2 30min \u5230 2 \u5c0f\u65f6\uff08\u5177\u4f53\u9700\u8981\u9884\u5b9e\u9a8c\uff09\uff0c\u6e7f\u76d2\u5185\u52a0\u5c11\u91cf\u6c34\u4fdd\u6301\u6e7f\u5ea6\u3002<\/p>\n\n\n\n<p><strong>\u767d\u5149 tunel\uff0c\u6309\u4ee5\u4e0b\u64cd\u4f5c\uff1a<\/strong>HRP \u4e8c\u6297\u5b75\u80b2\u4ee5\u53ca DAB \u663e\u8272\uff1a\u73bb\u7247\u7f6e\u4e8e PBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da4 3 \u6b21\uff0c\u6bcf\u6b21 5min\uff0c\u7136\u540e HRP \u4e8c\u6297\u5de5\u4f5c\u6db2\u5ba4\u6e29\u5b75\u80b2 25min\uff08HRP \u4e8c\u6297\u5de5\u4f5c\u6db2\uff0cStreptavidin-HRP\uff1aPBST=1:1000\uff09\uff0c\uff1b\u73bb\u7247\u7f6e\u4e8e PBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da4 3 \u6b21\uff0c\u6bcf\u6b21 5min\uff1bDAB \u53cd\u5e94\u6db2\uff08\u5ba2\u6237\u81ea\u5907\uff09\u663e\u8272;\u9152\u7cbe\u8131\u6c34\uff0c\u4e8c\u7532\u82ef\u900f\u660e\uff0c\u4e2d\u6027\u6811\u80f6\u5c01\u7247\u3002<\/p>\n\n\n\n<p><strong>\u5c01\u7247\uff1a<\/strong>\u73bb\u7247\u7f6e\u4e8e PBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da4 3 \u6b21\uff0c\u6bcf\u6b21 5min\u3002\u5207\u7247\u7a0d\u7529\u5e72\u540e\u7528\u6297\u8367\u5149\u6dec\u706d\u5c01\u7247\u5242\u5c01\u7247\u3002<\/p>\n\n\n\n<p><strong>\u955c\u68c0\u62cd\u7167\uff1a<\/strong>\u5207\u7247\u4e8e\u5c3c\u5eb7\u5012\u7f6e\u8367\u5149\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u5e76\u91c7\u96c6\u56fe\u50cf\u3002\uff08\u7d2b\u5916\u6fc0\u53d1\u6ce2\u957f 330-380nm\uff0c\u53d1\u5c04\u6ce2\u957f 420nm\uff1bFITC \u7eff\u5149\u6fc0\u53d1\u6ce2\u957f 465-495nm\uff0c\u53d1\u5c04\u6ce2\u957f 515-555 nm\uff1bCY3 \u7ea2\u5149\u6fc0\u53d1\u6ce2\u957f510-560\uff0c\u53d1\u5c04\u6ce2\u957f 590nm\uff09<\/p>\n\n\n\n<p><strong>\u77f3\u8721\u5207\u7247\u767d\u5149 tunel \u7ed3\u679c\u5224\u8bfb <\/strong>\u767d\u5149 tunel \u9633\u6027\u8868\u8fbe\u4e3a\u9ec4\u8272\u81f3\u68d5\u9ed1\u8272\uff0c\u6b63\u5e38\u7ec6\u80de\u4e3a\u84dd\u8272\u3002<\/p>\n\n\n\n<p><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><\/p>\n\n\n\n<p>1. \u672c\u8bd5\u5242\u4ec5\u4f9b\u79d1\u7814\u3002<\/p>\n\n\n\n<p>2. \u64cd\u4f5c\u65f6\u8bf7\u7a7f\u5b9e\u9a8c\u670d\uff0c\u4f69\u6234\u4e00\u6b21\u6027\u624b\u5957\u3002<\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-360d03a8-4400-4aa3-b4ef-4fc1a277059c\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20241104114814477.pdf\">\u767d\u5149tunel\u68c0\u67e5\u8bd5\u5242\u76d2\uff08DAB) HKI0012  \u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20241104114814477.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-360d03a8-4400-4aa3-b4ef-4fc1a277059c\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div 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