{"id":4274,"date":"2023-07-13T11:21:20","date_gmt":"2023-07-13T03:21:20","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=4274"},"modified":"2024-10-24T16:34:58","modified_gmt":"2024-10-24T08:34:58","slug":"30%e4%b8%99%e7%83%af%e9%85%b0%e8%83%ba291-2","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/4274.html","title":{"rendered":"30%\u4e19\u70ef\u9170\u80fa(29:1)"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/1.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/1.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"30%\u4e19\u70ef\u9170\u80fa(29:1)\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">30%\u4e19\u70ef\u9170\u80fa(29:1)<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/blxingtai\/xtgdy\/\">\u56fa\u5b9a\u6db2<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/blxingtai\/\">\u75c5\u7406\u5f62\u6001<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a550\/\u00a5200<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100ml\/500ml<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKW2027<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u74f6<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><th class=\"has-text-align-center\" data-align=\"center\"><span style=\"font-size: 24px; color: #333333;\"><strong>\u54c1\u724c<\/strong><\/span><\/th><th class=\"has-text-align-center\" data-align=\"center\"><span style=\"font-size: 24px; color: #333333;\"><strong>\u5546\u54c1\u7f16\u7801<\/strong><\/span><\/th><th class=\"has-text-align-center\" data-align=\"center\"><span style=\"font-size: 24px; color: #333333;\"><strong>\u5546\u54c1\u540d\u79f0<\/strong><\/span><\/th><th class=\"has-text-align-center\" data-align=\"center\"><span style=\"font-size: 24px; color: #333333;\"><strong>\u89c4\u683c<\/strong><\/span><\/th><th class=\"has-text-align-center\" data-align=\"center\"><span style=\"font-size: 24px; color: #333333;\"><strong>\u4ef7\u683c<\/strong><\/span><\/th><\/tr><tr><th class=\"has-text-align-center\" data-align=\"center\"><span style=\"color: #333333;\">HaoKebio<\/span><\/th><th class=\"has-text-align-center\" data-align=\"center\">HKW2027-100<\/th><th class=\"has-text-align-center\" data-align=\"center\">30%\u4e19\u70ef\u9170\u80fa(29:1)<\/th><th class=\"has-text-align-center\" data-align=\"center\">100ml<\/th><th class=\"has-text-align-center\" data-align=\"center\">\uffe550<\/th><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\"><span style=\"color: #333333;\"><strong>HaoKebio<\/strong><\/span><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>HKW2027-500<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>30%\u4e19\u70ef\u9170\u80fa(29:1)<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>500ml<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\uffe5200<\/strong><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>\u4ea7\u54c1\u7b80\u4ecb<\/strong><strong><\/strong><\/p>\n\n\n\n<p>30%&nbsp;Acr-Bis&nbsp;(29:1)\u5373\u542b30%\u4e19\u70ef\u9170\u80fa\u5236\u80f6\u6db2\u7684\u6c34\u6eb6\u6db2\uff0c\u5176\u4e2d\u4e19\u70ef\u9170\u80fa\u548c\u7532\u53c9\u53cc\u4e19\u70ef\u9170\u80fa\u7684\u6bd4\u4f8b\u4e3a29\uff1a1\u3002\u5e38\u7528\u4e8e\u914d\u5236PAGE\u51dd\u80f6\u548cSDS-PAGE\u51dd\u80f6\u7b49\uff0c\u53ef\u7528\u4e8e\u86cb\u767d\u6216\u6838\u9178\u7684\u5206\u79bb\uff0c\u662f\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u5ba4\u5e38\u7528\u7684\u5236\u80f6\u50a8\u5907\u6db2\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>\u50a8\u5b58\u4e0e\u8fd0\u8f93<\/strong><strong><\/strong><\/p>\n\n\n\n<p>\u51b0\u888b\uff08wet ice\uff09\u8fd0\u8f93\uff1b4\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u6709\u6548\u671f12\u4e2a\u6708\u3002<\/p>\n\n\n\n<p><strong>\u4f7f\u7528\u65b9\u6cd5<\/strong><strong><\/strong><\/p>\n\n\n\n<p>1.&nbsp;\u86cb\u767d\u5206\u5b50\u91cf\u4e0e\u51dd\u80f6\u6d53\u5ea6\u7684\u9009\u62e9\u53ef\u53c2\u8003\u4e0b\u8868\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>\u86cb\u767d\u8d28\u5206\u5b50\u91cf\u8303\u56f4\uff08kDa\uff09<\/strong><strong><\/strong><\/td><td><strong>\u9002\u5b9c\u7684\u51dd\u80f6\u6d53\u5ea6\uff08%\uff09<\/strong><strong><\/strong><\/td><\/tr><tr><td>\uff1c10<\/td><td>15-20<\/td><\/tr><tr><td>10-40<\/td><td>12-15<\/td><\/tr><tr><td>40-100<\/td><td>10-12<\/td><\/tr><tr><td>100-500<\/td><td>5-10<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>2.&nbsp;\u6839\u636e\u76ee\u6807\u86cb\u767d\u5206\u5b50\u91cf\u5927\u5c0f\uff0c\u9009\u53d6\u5206\u79bb\u80f6\u7684\u6d53\u5ea6\uff0c\u53ef\u53c2\u8003\u4e0b\u8868\u914d\u5236\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>\u5206\u79bb\u80f6\u6d53\u5ea6<\/strong><strong>(%)<\/strong><strong><\/strong><\/td><td><strong>8%<\/strong><strong><\/strong><\/td><td><strong>10%<\/strong><strong><\/strong><\/td><td><strong>12%<\/strong><strong><\/strong><\/td><td><strong>15%<\/strong><strong><\/strong><\/td><td><strong>18%<\/strong><strong><\/strong><\/td><td><strong>20%<\/strong><strong><\/strong><\/td><\/tr><tr><td>H2O\/mL<\/td><td>4.63<\/td><td>3.97<\/td><td>3.3<\/td><td>2.3<\/td><td>1.3<\/td><td>0.63<\/td><\/tr><tr><td>30%&nbsp;Acr-Bis&nbsp;(29:1)\/mL<\/td><td>2.67<\/td><td>3.33<\/td><td>4.0<\/td><td>5.0<\/td><td>6.0<\/td><td>6.67<\/td><\/tr><tr><td>1.5M Tris-HCl\uff08pH&nbsp;8.8\uff09\/mL<\/td><td>2.5<\/td><td>2.5<\/td><td>2.5<\/td><td>2.5<\/td><td>2.5<\/td><td>2.5<\/td><\/tr><tr><td>10%SDS\/\u03bcL<\/td><td>100<\/td><td>100<\/td><td>100<\/td><td>100<\/td><td>100<\/td><td>100<\/td><\/tr><tr><td>AP\uff08\u8fc7\u786b\u9178\u94f5\uff09\/\u03bcL<\/td><td>100<\/td><td>100<\/td><td>100<\/td><td>100<\/td><td>100<\/td><td>100<\/td><\/tr><tr><td>TEMED\/\u03bcL<\/td><td>5<\/td><td>5<\/td><td>5<\/td><td>5<\/td><td>5<\/td><td>5<\/td><\/tr><tr><td>\u603b\u4f53\u79ef*\/mL<\/td><td colspan=\"6\">10<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>*\u603b\u4f53\u79ef\u4e0d\u5305\u542bTEMED\u7684\u4f53\u79ef\u3002<\/p>\n\n\n\n<p>3.&nbsp;\u6d53\u7f29\u80f6\u914d\u5236\u53ef\u53c2\u8003\u4e0b\u8868\uff1a<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>\u6d53\u7f29\u80f6\u6d53\u5ea6<\/strong><strong>(%)<\/strong><strong><\/strong><\/td><td colspan=\"4\"><strong>5%<\/strong><strong><\/strong><\/td><\/tr><tr><td>H2O\/mL<\/td><td>1.93<\/td><td>2.89<\/td><td>3.86<\/td><td>5.79<\/td><\/tr><tr><td>30%&nbsp;Acr-Bis&nbsp;(29:1)\/mL<\/td><td>0.5<\/td><td>0.75<\/td><td>1.0<\/td><td>1.5<\/td><\/tr><tr><td>1.0M Tris-HCl\uff08pH&nbsp;6.8\uff09\/mL<\/td><td>0.5<\/td><td>0.75<\/td><td>1.0<\/td><td>1.5<\/td><\/tr><tr><td>10%SDS\/\u03bcL<\/td><td>40<\/td><td>60<\/td><td>80<\/td><td>120<\/td><\/tr><tr><td>AP\uff08\u8fc7\u786b\u9178\u94f5\uff09\/\u03bcL<\/td><td>30<\/td><td>45<\/td><td>60<\/td><td>90<\/td><\/tr><tr><td>TEMED\/\u03bcL<\/td><td>4<\/td><td>6<\/td><td>8<\/td><td>12<\/td><\/tr><tr><td>\u603b\u4f53\u79ef*\/mL<\/td><td>3<\/td><td>4.5<\/td><td>6<\/td><td>9<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>*\u603b\u4f53\u79ef\u4e0d\u5305\u542bTEMED\u7684\u4f53\u79ef\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>\u6ce8\u610f\u4e8b\u9879<\/strong><\/p>\n\n\n\n<p>1.&nbsp;\u672c\u54c1\u6709\u4e00\u5b9a\u7684\u6bd2\u6027\uff0c\u4f7f\u7528\u65f6\u8bf7\u6ce8\u610f\u9632\u62a4\u3002<\/p>\n\n\n\n<p>2.&nbsp;\u6e29\u5ea6\u5bf9\u4e8e\u80f6\u7684\u51dd\u56fa\u65f6\u95f4\u4f1a\u6709\u5f71\u54cd\uff0c\u4e00\u822c\u6e29\u5ea6\u8d8a\u4f4e\uff0c\u51dd\u56fa\u65f6\u95f4\u8d8a\u957f\uff1b\u6e29\u5ea6\u8d8a\u9ad8\u51dd\u80f6\u8d8a\u5feb\u3002<\/p>\n\n\n\n<p>3.&nbsp;\u51dd\u80f6\u9700\u8981\u5145\u5206\u7684\u51dd\u56fa\u65f6\u95f4\uff0c\u5efa\u8bae\u80f6\u5236\u597d\u5145\u5206\u9759\u7f6e\u786e\u4fdd\u51dd\u80f6\u5f7b\u5e95\u3002<\/p>\n\n\n\n<p>4.&nbsp;\u5982\u9700\u5236\u80f6\u5168\u5957\u8bd5\u5242\u53ef\u8d2d\u4e70\u672c\u516c\u53f8\u5176\u4ed6\u4ea7\u54c1\uff0c\u5982SDS-PAGE\u51dd\u80f6\u5236\u5907\u8bd5\u5242\u76d2\u6216\u8d85\u5feb\u901f\u5f69\u8272\u51dd\u80f6\u914d\u5236\u8bd5\u5242\u76d2\u7cfb\u5217\u6216\u8d85\u5feb\u901f\u5f69\u8272\u51dd\u80f6\u914d\u5236\u8bd5\u5242\u76d2\u7cfb\u5217\uff0c\u53ca\u9ad8\u5206\u8fa8\u8d85\u5feb\u901f\u5f69\u8272\u51dd\u80f6\u914d\u5236\u8bd5\u5242\u76d2\u7cfb\u5217\uff0c\u53ef\u6ee1\u8db3\u4e0d\u540c\u5b9e\u9a8c\u9700\u6c42\u3001<\/p>\n\n\n\n<p>5.&nbsp;\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<\/p>\n\n\n\n<p><strong>\u4ea7\u54c1\u4f9b\u79d1\u7814\u7528\u9014\uff0c\u4e0d\u7528\u4e8e\u4e34\u5e8a\u8bca\u65ad\uff01<\/strong><\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-db45662f-c0d0-49b4-ad72-d79ce5ec06e8\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20241010140232303.pdf\">30%\u4e19\u7a00\u9170\u80fa(29-1) HKW2027 \u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20241010140232303.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-db45662f-c0d0-49b4-ad72-d79ce5ec06e8\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide 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