{"id":4189,"date":"2023-07-12T13:35:46","date_gmt":"2023-07-12T05:35:46","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=4189"},"modified":"2024-10-10T14:07:09","modified_gmt":"2024-10-10T06:07:09","slug":"edu%e6%9f%93%e8%89%b2%e8%af%95%e5%89%82%e7%9b%92%ef%bc%88%e7%bb%bf%ef%bc%8caf488","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/4189.html","title":{"rendered":"Edu\u67d3\u8272\u8bd5\u5242\u76d2\uff08\u7eff\uff0cAF488)"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/EDU\u67d3\u8272\u8bd5\u5242\u76d2-hki0021-1.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/EDU\u67d3\u8272\u8bd5\u5242\u76d2-hki0021-1.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"Edu\u67d3\u8272\u8bd5\u5242\u76d2\uff08\u7eff\uff0cAF488)\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">Edu\u67d3\u8272\u8bd5\u5242\u76d2\uff08\u7eff\uff0cAF488)<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/cpzh\/\">\u5206\u5b50\u75c5\u7406<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/cpzh\/hkygrl\/\">\u8367\u5149\u67d3\u6599<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a5700<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100T<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKI0021<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u4e2a<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><th>\u54c1\u724c<\/th><th>\u5546\u54c1\u7f16\u7801<\/th><th>\u5546\u54c1\u540d\u79f0<\/th><th>\u89c4\u683c<\/th><th>\u4ef7\u683c<\/th><\/tr><tr><th><span style=\"color: #333333;\">HaoKebio<\/span><\/th><th>HKI0021<\/th><th>Edu\u67d3\u8272\u8bd5\u5242\u76d2\uff08\u7eff\uff0cAF488)<\/th><th>100T<\/th><th>\uffe5700<\/th><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br><br><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<br><\/strong>EdU(5-ethynyl-2&#8242; -deoxyuridine)\uff0c\u4e2d\u6587\u540d\u4e3a 5-\u4e59\u7094\u57fa-2&#8242; -\u8131\u6c27\u5c3f\u82f7\uff0c\u662f\u4e00\u79cd\u80f8\u817a\u5627\u5576 \u6838\u82f7\u7c7b\u4f3c\u7269\uff0c\u5728\u7ec6\u80de\u589e\u6b96\u65f6\u80fd\u591f\u66ff\u4ee3\u80f8\u82f7(\u80f8\u817a\u5627\u5576\u8131\u6c27\u6838\u82f7\uff0cthymidine) \u63d2\u5165\u6b63\u5728\u590d\u5236\u7684 DNA&nbsp;\u5206\u5b50\u4e2d\uff0c &nbsp;EdU \u4e0a\u7684\u4e59\u7094\u57fa\u80fd\u4e0e\u8367\u5149\u6807\u8bb0\u7684\u5c0f\u5206\u5b50\u53e0\u6c2e\u5316\u7269\u63a2\u9488(\u5982Azide&nbsp;Alexa&nbsp;Fluor&nbsp;488 \u7b49)&nbsp;\u901a\u8fc7\u4e00\u4ef7\u94dc\u79bb\u5b50\u50ac\u5316\u53d1\u751f\u5171\u4ef7\u53cd\u5e94\uff0c\u5f62\u6210\u7a33\u5b9a\u7684\u4e09\u5511\u73af\uff0c\u8be5\u53cd\u5e94\u975e\u5e38\u8fc5\u901f\uff0c\u88ab\u79f0\u4f5c\u70b9\u51fb\u53cd\u5e94&nbsp;(Click&nbsp;reaction)\uff0c\u4ece\u800c\u53ef\u4ee5\u8fdb\u884c\u9ad8\u6548\u5feb\u901f\u7684\u68c0\u6d4b\u7ec6\u80de\u589e\u6b96\uff0c\u7279\u522b\u662f\u53ef\u4ee5\u6709\u6548\u5730\u68c0\u6d4b\u5904\u4e8e S \u671f \u7684\u7ec6\u80de\u767e\u5206\u6570\u3002<br>\u672c\u8bd5\u5242\u76d2\u4e2d\u8367\u5149\u63a2\u9488\u4e3a\u7eff\u8272\u8367\u5149\uff0c\u6700\u5927\u6fc0\u53d1\u6ce2\u957f\u4e3a 491 nm\uff0c\u6700\u5927\u53d1\u5c04\u6ce2\u957f\u4e3a 516 nm\uff0c\u5904&nbsp;\u4e8e\u589e\u6b96\u7684\u7ec6\u80de\u88ab\u6807\u8bb0\u540e\uff0c\u7ec6\u80de\u6838\u4f1a\u663e\u793a\u51fa\u660e\u4eae\u7684\u7eff\u8272\u8367\u5149\uff0c\u901a\u8fc7\u914d\u5957\u5e38\u89c4\u7ec6\u80de\u6838\u67d3\u6599\u5171\u540c\u6807\u8bb0&nbsp;\u7ec6\u80de\u6838\uff0c\u53ef\u7528\u8367\u5149\u663e\u5fae\u955c\u3001\u6fc0\u5149\u5171\u805a\u7126\u663e\u5fae\u955c\u7b49\u4eea\u5668\u76f4\u63a5\u89c2\u5bdf\u7ec6\u80de\u589e\u6b96\u60c5\u51b5\uff1b\u4e5f\u53ef\u4ee5\u4f7f\u7528\u6d41\u5f0f&nbsp;\u7ec6\u80de\u4eea\u68c0\u6d4b\u4f53\u5916\u57f9\u517b\u7ec6\u80de\u7fa4\u8367\u5149\u5f3a\u5ea6\uff0c\u6839\u636e\u8367\u5149\u5f3a\u5ea6\uff0c\u6765\u5224\u65ad\u7ec6\u80de\u5468\u671f\u4e2d S&nbsp;\u671f\u7684 DNA&nbsp;\u590d\u5236\u6d3b\u6027\u3002<br><br><strong>\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/strong><br>\u51b0\u888b\u8fd0\u8f93\uff0c-20\u02daC&nbsp;\u50a8\u5b58\uff0c\u53ef\u7a33\u5b9a\u50a8\u5b58\u4e00\u5e74<\/figcaption><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><\/p>\n\n\n\n<p>\u52a8\u7269&nbsp;EdU&nbsp;\u6ce8\u5c04<\/p>\n\n\n\n<p>\u5bf9\u4e8e\u5c0f\u9f20\uff0c\u53ef\u4ee5\u6309\u7167 10-200mg\/kg \u7684\u7528\u91cf\uff0c\u628a EdU \u7528 PBS \u914d\u5236\u6210\u4e00\u5b9a\u6d53\u5ea6\uff0c\u8179\u8154\u6ce8\u5c04\u3001\u7279\u5b9a\u7ec4&nbsp;\u7ec7\u6216\u5668\u5b98\u5c40\u90e8\u6ce8\u5c04\u6216\u8005\u52a0\u5165\u996e\u7528\u6c34\u4e2d\u3002\u5177\u4f53\u7528\u91cf\u8ddf\u6240\u7528\u52a8\u7269\u7684\u79cd\u7c7b\u3001\u4f53\u91cd\u548c\u4f7f\u7528\u65b9\u5f0f\u6709\u5173\uff0c\u53ef \u4ee5\u53c2\u8003\u76f8\u5173\u6587\u732e\uff0c\u56e0\u6b64\u521d\u6b21\u4f7f\u7528\u65f6\u5efa\u8bae\u5bf9 EdU \u7684\u4f7f\u7528\u6d53\u5ea6\u8fdb\u884c\u4e00\u5b9a\u7684\u6478\u7d22\uff0c\u6216\u8005\u76f4\u63a5\u4f7f 50mg\/kg&nbsp;\u7684\u6d53\u5ea6\u8fdb\u884c\u6d4b\u8bd5\u3002<\/p>\n\n\n\n<p>\u6ce8\u5c04\u65b9\u5f0f:\u4f9d\u636e\u5ba2\u6237\u5b9e\u9a8c\u800c\u5b9a\uff0c\u5982\u8179\u8154\u6ce8\u5c04\uff0c\u76ae\u4e0b\u6ce8\u5c04\uff0c\u808c\u8089\u6ce8\u5c04\uff0c\u5c3e\u9759\u8109\u6ce8\u5c04\u7b49\uff0c\u5176\u4e2d\u4ee5\u8179\u8154 \u6ce8\u5c04\u4e3a\u591a\u30026 \u5c0f\u65f6\u540e\u6216\u6839\u636e\u7279\u5b9a\u5b9e\u9a8c\u786e\u5b9a\u9002\u5f53\u7684\u65f6\u95f4\u540e\uff0c\u5feb\u901f\u5904\u6b7b\u5c0f\u9f20\uff0c\u53d6\u51fa\u6240\u9700\u7ec4\u7ec7\uff0c\u6309\u7167 \u5e38\u89c4\u6b65\u9aa4\u5236\u4f5c\u51b0\u51bb\u5207\u7247\u6216\u77f3\u8721\u5207\u7247\u3002EdU&nbsp;\u6807\u8bb0\u7684\u65f6\u5019\u4e5f\u53ef\u53c2\u8003\u76f8\u5173\u6587\u732e\u81ea\u884c\u8c03\u6574\u3002\u5efa\u8bae\u4efb\u4f55\u5b9e \u9a8c\u5747\u53ef\u53d6\u5c0f\u80a0\u7ec4\u7ec7\u68c0\u6d4b\u7ec6\u80de\u589e\u6b96\u60c5\u51b5\uff0c\u5c0f\u80a0\u4e0a\u76ae\u7ec4\u7ec7\u7ec6\u80de\u589e\u6b96\u5feb\uff0c\u6210\u5e74\u5c0f\u9f20 EdU \u6ce8\u5c04 6 \u5c0f\u65f6\u540e&nbsp;\u5373\u53ef\u68c0\u6d4b\u5230\u9633\u6027\u4fe1\u606f\uff0c\u53ef\u7528\u4f5c\u9633\u6027\u5bf9\u7167\u8fdb\u884c\u9884\u5b9e\u9a8c\u3002<\/p>\n\n\n\n<p>\u5207\u7247\u5904\u7406<\/p>\n\n\n\n<p>\u5207\u7247\u524d\u5904\u7406\uff1a\u7ec4\u7ec7\u5668\u5b98\u6700\u597d\u8fdb\u884c\u6e05\u6d17\uff0c\u4ee5\u53bb\u9664\u8840\u6db2\u3001\u7ec4\u7ec7\u4e2d\u6b8b\u7559\u7684EdU\uff0c\u964d\u4f4e\u80cc\u666f\u3002<\/p>\n\n\n\n<p>\u5207\u7247\u539a\u5ea6\uff1a3-10\u03bcm \u4e3a\u5b9c\uff0c\u5207\u7247\u8fc7\u539a\u53ef\u80fd\u4f1a\u5f71\u54cd\u5207\u7247\u80cc\u666f\u3002<\/p>\n\n\n\n<p>\u5207\u7247\u540e\u5904\u7406\uff1a<\/p>\n\n\n\n<p>\u77f3\u8721\u5207\u7247\u8131\u8721\uff1a\u4e8c\u7532\u82ef\u8131\u8721 2 \u6b21\uff0c15 \u5206\u949f\/\u6b21\uff0c\u4e59\u9187\u68af\u5ea6 (100%\uff0c95%\uff0c85%\uff0c75%) \u6d17\u8131\u5404\u4e00\u6b21\uff0c&nbsp;5&nbsp;\u5206\u949f\/\u6b21\uff0c\u53bb\u79bb\u5b50\u6c34\u6d17\u8131 1 \u6b21\u3002<\/p>\n\n\n\n<p>\u51b0\u51bb\u5207\u7247\u5904\u7406\uff1a\u5ba4\u6e29\u653e\u7f6e 30 \u5206\u949f\u540e\uff0c\u56fa\u5b9a 10 \u5206\u949f\uff0cPBS \u6e05\u6d17 3 \u6b21\uff0c\u6bcf\u6b21 5 \u5206\u949f\u3002<\/p>\n\n\n\n<p>Edu&nbsp;\u53cd\u5e94<\/p>\n\n\n\n<p>\u6309\u7167&nbsp;PB:CU:\u7eff\u8272\u8272\u539f\uff1aAC=860:40:1:100 \u7684\u6bd4\u5217\u914d\u5236 EdU \u53cd\u5e94\u6db2\u3002 &nbsp;(\u53cd\u5e94\u6db2\u73b0\u914d\u73b0\u7528)<\/p>\n\n\n\n<p>\u6bcf\u7247\u7ec4\u7ec7\u6ef4\u52a0 50-100 \u03bcl \u7684 EdU \u67d3\u8272\u53cd\u5e94\u6db2\uff0c\u5ba4\u6e29\u907f\u5149\u5b75\u80b2 15 \u5206\u949f\uff0c\u5f03\u67d3\u8272\u53cd\u5e94\u6db2\uff0cPBS \u51b2\u6d17&nbsp;3&nbsp;\u6b21\uff0c\u6bcf\u6b21 5 \u5206\u949f\u3002<\/p>\n\n\n\n<p>\u5176\u4ed6\u67d3\u8272<\/p>\n\n\n\n<p>\u5ba2\u6237\u53ef\u4ee5\u6839\u636e\u9700\u8981\u8fdb\u884c\u7ec6\u80de\u8868\u9762\u6216\u8005\u7ec6\u80de\u5185\u6297\u539f\u67d3\u8272\u3002<\/p>\n\n\n\n<p>DAPI&nbsp;\u67d3\u8272<\/p>\n\n\n\n<p>\u6bcf\u7247\u7ec4\u7ec7\u52a0\u5165&nbsp;50-100ul DAPI \u67d3\u8272\u6db2\uff0c\u67d3\u8272 15 \u5206\u949f\uff0cPBS \u6d17\u8131\u4e09\u6b21\uff0c\u6bcf\u6b21 5 \u5206\u949f\u3002<\/p>\n\n\n\n<p>\u56fe\u50cf\u62cd\u7167<\/p>\n\n\n\n<p>\u67d3\u8272\u5b8c\u6210\u540e\u5efa\u8bae\u7acb\u523b\u89c2\u5bdf\uff0c\u4f7f\u7528\u8367\u5149\u663e\u5fae\u955c\u6216\u5171\u805a\u7126\u663e\u5fae\u955c\u91c7\u96c6\u56fe\u50cf\uff0c\u672c\u8bd5\u5242\u76d2\u8367\u5149\u67d3\u6599 AF488&nbsp;\u5bf9\u5e94\u7684\u5149\u8c31\u7279\u6027\u4e3a Ex\/Em\uff1a491 nm\/516&nbsp;nm (\u7eff\u8272) \uff1bDAPI \u67d3\u8272\u6db2\u5bf9\u5e94\u7684\u5149\u8c31\u7279\u6027\u4e3a Ex\/Em\uff1a<\/p>\n\n\n\n<p>358 nm\/461 nm&nbsp;(\u84dd\u8272) \u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><\/p>\n\n\n\n<p>1. \u5bf9\u4e8e\u4f53\u5916\u57f9\u517b\u7ec6\u80de\uff0c\u5177\u4f53 EdU&nbsp;\u4f7f\u7528\u6d53\u5ea6\u3001\u5b75\u80b2\u65f6\u95f4\u968f\u6837\u54c1\u4ee5\u53ca\u7814\u7a76\u76ee\u7684\u7684\u4e0d\u540c\uff0c\u53ef\u8fdb\u884c \u9002\u5f53\u8c03\u6574\u3002<\/p>\n\n\n\n<p>2. \u90e8\u5206\u7ec4\u7ec7\u7ec6\u80de\u589e\u6b96\u901f\u5ea6\u7f13\u6162\uff0c\u4e3a\u4e86\u6392\u9664\u9020\u6a21\u6548\u679c\u4e0d\u4f73\u7b49\u56e0\u7d20\uff0c\u5efa\u8bae\u9009\u589e\u6b96\u5feb\u7684\u7ec4\u7ec7\u6837\u54c1\u4f5c \u4e3a\u53c2\u7167\u6837\u672c (\u5982\u80a0\u9053\u7ec4\u7ec7)\u3002<\/p>\n\n\n\n<p>3. \u5982\u679c\u80cc\u666f\u989c\u8272\u8fc7\u6df1\uff0c\u53ef\u80fd\u662f\u5b9e\u9a8c\u4e2d\u6d17\u6da4\u4e0d\u5145\u5206\u3001\u7ec4\u7ec7\u6837\u54c1\u56fa\u5b9a\u65f6\u95f4\u8fc7\u957f\u3001\u56fa\u5b9a\u6db2\u6b8b\u4f59\u5bfc\u81f4\u3002<\/p>\n\n\n\n<p>4. \u50ac\u5316\u5242 AC 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