{"id":4178,"date":"2023-07-12T11:37:29","date_gmt":"2023-07-12T03:37:29","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=4178"},"modified":"2024-09-18T10:36:24","modified_gmt":"2024-09-18T02:36:24","slug":"bca%e8%9b%8b%e7%99%bd%e5%ae%9a%e9%87%8f%e8%af%95%e5%89%82%e7%9b%92","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/4178.html","title":{"rendered":"BCA\u86cb\u767d\u5b9a\u91cf\u8bd5\u5242\u76d2"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/BCA\u86cb\u767d\u5b9a\u91cf\u8bd5\u5242\u76d2hk2019.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/BCA\u86cb\u767d\u5b9a\u91cf\u8bd5\u5242\u76d2hk2019.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"BCA\u86cb\u767d\u5b9a\u91cf\u8bd5\u5242\u76d2\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">BCA\u86cb\u767d\u5b9a\u91cf\u8bd5\u5242\u76d2<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/wb\/\">\u86cb\u767d\u8d28\u751f\u7269\u5b66<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a5350<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">500\u6b21<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKW2019<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u74f6<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><td>\u4ea7\u54c1\u76ee\u5f55<\/td><td>\u4e3b\u8981\u6210\u5206<\/td><td>\u4ea7\u54c1\u89c4\u683c<\/td><\/tr><tr><td>HKW2019-1<\/td><td>\u00a0BCA \u8bd5\u5242<\/td><td>100mL<\/td><\/tr><tr><td>HKW2019-2<\/td><td>\u00a0\u786b\u9178\u94dc\u6eb6\u6db2<\/td><td>3\u00d71.2mL<\/td><\/tr><tr><td>HKW2019-3<\/td><td>\u86cb\u767d\u6807\u51c6\u54c1\uff08BSA\uff09<\/td><td>3\u00d725mg<\/td><\/tr><tr><td>HKW2019-4<\/td><td>\u86cb\u767d\u6807\u51c6\u914d\u5236\u6db2<\/td><td>3\u00d71.2mL<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><br><br>BCA&nbsp;\u6cd5\u5b9a\u91cf\u68c0\u6d4b\u86cb\u767d\u7684\u57fa\u672c\u539f\u7406\u662f\u57fa\u4e8e\u53cc\u7f29\u8132\u53cd\u5e94\uff0c\u5373\u5728\u78b1\u6027\u6761\u4ef6\u4e0b\uff0cCu2+\u88ab\u86cb\u767d\u8d28\u8fd8\u539f&nbsp;\u6210 Cu+\uff0c\u968f\u540e Cu+\u4e0e BCA (Bicinchonic acid\uff0c &nbsp;\u4e00\u79cd\u663e\u8272\u5242) \u53d1\u751f\u87af\u5408\u53cd\u5e94\uff0c\u6bcf\u4e24\u5206\u5b50 BCA \u87af&nbsp;\u5408\u4e00\u4e2a Cu+\uff0c\u751f\u6210\u7d2b\u8272\u7684\u6c34\u6eb6\u6027\u590d\u5408\u7269\uff0c\u8be5\u7269\u8d28\u5728 562 nm&nbsp;\u5904\u6709\u6700\u9ad8\u5438\u6536\u503c\uff0c\u4e14\u989c\u8272\u6df1\u6d45\u4e0e\u86cb&nbsp;\u767d\u8d28\u6d53\u5ea6\u4e00\u5b9a\u8303\u56f4\u5185\u6210\u6b63\u6bd4\uff0c\u56e0\u6b64\u53ef\u7528\u4e8e\u86cb\u767d\u8d28\u7684\u5b9a\u91cf\u68c0\u6d4b\uff0c\u86cb\u767d\u6d53\u5ea6\u5728 50-2000 \u03bcg\/mL&nbsp;\u6d53&nbsp;\u5ea6\u8303\u56f4\u5185\u6709\u8f83\u597d\u7684\u7ebf\u6027\u5173\u7cfb\u3002<br>\u672c\u65b9\u6cd5\u4e0d\u53d7\u7edd\u5927\u90e8\u5206\u6837\u54c1\u4e2d\u5316\u5b66\u7269\u8d28\u7684\u5f71\u54cd\uff0c\u53ef\u4ee5\u517c\u5bb9\u6837\u54c1\u4e2d\u9ad8\u6d53\u5ea6\u7684\u53bb\u57a2\u5242\uff0c\u5305\u62ec\u6d53\u5ea6&nbsp;\u9ad8\u8fbe 5%\u7684 SDS\uff0c5%\u7684 Triton&nbsp;X-100\uff0c5%\u7684 Tween-20\u300160\u300180 \u7b49\u3002\u4f46\u87af\u5408\u5242\u548c\u9ad8\u6d53\u5ea6\u8fd8\u539f\u5242&nbsp;\u4f1a\u5f71\u54cd\u68c0\u6d4b\u7ed3\u679c\uff0c\u9700\u786e\u4fdd\u6837\u54c1\u4e2d\u65e0 EGTA\uff0cEDTA \u6d53\u5ea6\u4f4e\u4e8e 10 mM\uff0cDTT \u4f4e\u4e8e 1 mM\uff0c \u03b2-\u5def\u57fa\u4e59 \u9187\u4f4e\u4e8e 1&nbsp;mM\u3002<\/figcaption><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/strong><\/p>\n\n\n\n<p>\u51b0\u888b\u8fd0\u8f93\uff1b\u86cb\u767d\u6807\u51c6\u54c1 (BSA) 4\u2103\u4fdd\u5b58\uff0c\u6709\u6548\u671f 12 \u4e2a\u6708\u3002\u914d\u5236\u6210\u7684\u86cb\u767d\u6807\u51c6\u6eb6\u6db2\u540e\u9700&nbsp;-20\u2103 \u4fdd\u5b58\uff0c\u5e76\u5728 6 \u4e2a\u6708\u5185\u4f7f\u7528\u3002\u5176\u4f59\u8bd5\u5242\u5e38\u6e29\u4fdd\u5b58\uff0c\u6709\u6548\u671f 12 \u4e2a\u6708\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><\/p>\n\n\n\n<p>1. \u914d\u5236\u86cb\u767d\u6807\u51c6\u8d2e\u5907\u6db2\uff1a\u53d6 1 mL&nbsp;\u86cb\u767d\u6807\u51c6\u914d\u5236\u6db2\u52a0\u5165\u5230\u86cb\u767d\u6807\u51c6\u54c1 (BSA) \u86cb\u767d\u6807\u51c6\u7ba1<\/p>\n\n\n\n<p>\u4e2d\uff0c\u5c06 25 mg&nbsp;\u86cb\u767d\u6807\u51c6\u54c1\u5b8c\u5168\u6eb6\u89e3\uff0c\u5373\u5f97\u5230\u6d53\u5ea6\u4e3a 25 mg\/mL \u7684\u86cb\u767d\u6807\u51c6\u8d2e\u5907\u6db2\u3002\u914d\u5236\u6210\u7684\u86cb \u767d\u6807\u51c6\u6eb6\u6db2\u53ef-20\u2103 \u957f\u671f\u4fdd\u5b58\u3002<\/p>\n\n\n\n<p>2.&nbsp;\u914d\u5236\u86cb\u767d\u6807\u51c6\u5de5\u4f5c\u6db2\uff1a\u53d6\u9002\u91cf 25 mg\/mL \u86cb\u767d\u6807\u51c6\u8d2e\u5907\u6db2\uff0c\u7528 PBS \u6216\u751f\u7406\u76d0\u6c34\u7a00\u91ca 50<\/p>\n\n\n\n<p>\u500d\uff0c\u5f97\u5230\u7ec8\u6d53\u5ea6\u4e3a 0.5 mg\/mL \u7684\u86cb\u767d\u6807\u51c6\u5de5\u4f5c\u6db2\u3002\u6ce8\u610f\u7a00\u91ca\u65f6\u6309\u7167 10 \u500d\u68af\u5ea6\u65b9\u6cd5\u7a00\u91ca\uff0c\u786e\u4fdd \u7a00\u91ca\u51c6\u786e\u3002<\/p>\n\n\n\n<p>3. \u7ed8\u5236\u6807\u51c6\u66f2\u7ebf (\u9176\u6807\u4eea\u6cd5) \uff1a\u5c06\u86cb\u767d\u6807\u51c6\u5de5\u4f5c\u6db2\u5206\u522b\u6309 0\uff0c1\uff0c2\uff0c4\uff0c8\uff0c12\uff0c16\uff0c20 \u03bc&nbsp;L&nbsp;\u52a0\u5230 96 \u5b54 \u677f\u4e2d\uff0c\u7136\u540e\u7528 PBS \u6216\u751f\u7406\u76d0\u6c34\u4f9d\u6b21\u52a0 20\uff0c19\uff0c18\uff0c16\uff0c12\uff0c8\uff0c4\uff0c0 \u03bcL \u5c06\u4e0a \u8ff0\u68af\u5ea6\u5de5\u4f5c\u6db2\u8865\u8db3\u5230 20 \u03bcL\u3002\u5f97\u5230\u86cb\u767d\u6d53\u5ea6\u4f9d\u6b21\u4e3a 0\uff0c25\uff0c50\uff0c100\uff0c200\uff0c300\uff0c400\uff0c500 \u03bc g\/mL&nbsp;\u7684\u68af\u5ea6\u66f2\u7ebf\u3002<\/p>\n\n\n\n<p>4. \u51c6\u5907\u5f85\u6d4b\u6837\u54c1\uff1a\u5c06\u5f85\u6d4b\u7684\u86cb\u767d\u6837\u54c1\u8fdb\u884c\u9002\u5f53\u7a00\u91ca (\u53ef\u901a\u8fc7\u9884\u5b9e\u9a8c\u68c0\u6d4b\uff0c\u4f7f\u6837\u54c1\u86cb\u767d\u6d53\u5ea6 \u5728\u6807\u51c6\u66f2\u7ebf\u8303 \u56f4\u5185\uff0c\u786e\u4fdd\u68c0\u6d4b\u7ed3\u679c\u53ef\u4fe1) \uff0c\u6309\u7167\u6bcf\u4e2a\u6837\u54c1 20 \u03bcL \u7684\u91cf\u52a0\u5230 96 \u5b54\u677f\u4e2d\u3002\u5f85\u6d4b \u6837\u54c1\u4e0e\u86cb\u767d\u6807\u51c6\u54c1\u7528\u76f8&nbsp;\u540c\u6eb6\u6db2\u7a00\u91ca\u3002<\/p>\n\n\n\n<p>5. \u914d\u5236 BCA&nbsp;\u663e\u8272\u5de5\u4f5c\u6db2\uff1a\u5c06 BCA&nbsp;\u8bd5\u5242\u4e0e\u786b\u9178\u94dc\u6eb6\u6db2\u6309\u7167 50:1 \u4f53\u79ef\u6bd4\u5145\u5206\u6df7\u5408\u5747\u5300\uff0c&nbsp;\u5f97\u5230 BCA&nbsp;\u663e\u8272\u5de5\u4f5c\u6db2\u3002BCA&nbsp;\u663e\u8272\u5de5\u4f5c\u6db2\u53ef\u5ba4\u6e29\u4fdd\u5b58\uff0c24 h&nbsp;\u5185\u4f7f\u7528\u3002\u6bcf\u4e2a\u5f85\u6d4b\u6837\u54c1\u9700 200 \u03bcL \uff0c\u5efa\u8bae\u6309\u9700\u914d\u5236\uff0c\u907f\u514d\u6d6a\u8d39\u3002<\/p>\n\n\n\n<p>6. \u68c0\u6d4b\uff1a\u5411\u6807\u51c6\u66f2\u7ebf\u6837\u54c1\u5b54\u53ca\u5f85\u6d4b\u6837\u54c1\u5b54\u4e2d\u6bcf\u5b54\u52a0\u5165 BCA&nbsp;\u663e\u8272\u5de5\u4f5c\u6db2 200 \u03bcL\uff0c\u5145\u5206\u6df7&nbsp;\u5300&nbsp;(\u53ef\u5c06 96 \u5b54\u677f\u653e\u5728\u632f\u8361\u5668\u4e0a\u632f\u8361 30 s) \uff0c37\u2103\u53cd\u5e94 30 min&nbsp;\u540e\uff0c\u4ee5\u6807\u51c6\u66f2\u7ebf 0 \u53f7\u505a\u53c2\u6bd4&nbsp;\uff0c\u5728 562 nm&nbsp;\u6ce2\u957f\u4e0b\u6bd4\u8272\u6d4b\u5b9a\uff0c &nbsp;\u8bb0\u5f55\u5404\u5b54\u5438\u5149\u5ea6\u503c\u3002 &nbsp;(\u6ce8\uff1a\u4e5f\u53ef\u4ee5\u5728\u5ba4\u6e29\u53cd\u5e94 2 h\uff0c\u6216 60\u2103 \u53cd\u5e94 30 min\u3002\u5982\u679c\u86cb\u767d\u6d53\u5ea6\u8f83\u4f4e\uff0c\u5efa\u8bae\u7f6e \u4e8e 60\u2103\u53cd\u5e94)<\/p>\n\n\n\n<p>7. \u8ba1\u7b97\uff1a\u4ee5\u6807\u51c6\u66f2\u7ebf\u4e2d\u68af\u5ea6\u86cb\u767d\u542b\u91cf ( \u03bcg\/mL) \u4e3a\u6a2a\u5750\u6807\uff0c\u5438\u5149\u503c\u4e3a\u7eb5\u5750\u6807\uff0c\u7ed8\u51fa\u6807\u51c6&nbsp;\u66f2\u7ebf\u3002\u6839\u636e\u6240\u6d4b \u6837\u54c1\u7684\u5438\u5149\u503c\uff0c\u5728\u6807\u51c6\u66f2\u7ebf\u4e0a\u5373\u53ef\u67e5\u5f97\u76f8\u5e94\u5b54\u4e2d\u5f85\u6d4b\u6837\u54c1\u7684\u86cb\u767d\u6d53\u5ea6 ( \u03bcg\/mL&nbsp;) \uff0c\u518d\u4e58\u4ee5\u6837\u54c1\u7a00\u91ca\u500d\u6570\u5373\u4e3a\u5f85\u6d4b\u6837\u54c1\u5b9e\u9645\u86cb\u767d\u6d53\u5ea6\u3002\u8bae\u6309\u9700\u914d\u5236\uff0c\u907f\u514d\u6d6a\u8d39\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><\/p>\n\n\n\n<p>1.BCA&nbsp;\u6cd5\u6d4b\u5b9a\u86cb\u4f7f\u7528 BCA&nbsp;\u86cb\u767d\u6d4b\u5b9a\u6cd5\u6d4b\u5b9a\u65f6\u989c\u8272\u4f1a\u968f\u7740\u65f6\u95f4\u7684\u5ef6\u957f\u4e0d\u65ad\u52a0\u6df1\uff0c\u5e76\u4e14\u663e\u8272&nbsp;\u53cd\u5e94\u7684\u901f\u5ea6\u548c\u6e29\u5ea6\u6709\u5173\uff0c\u56e0\u6b64\u9700\u6ce8\u610f\u4fdd\u6301\u5b9a\u65f6\u548c\u5b9a\u6e29\uff0c\u4ee5\u786e\u4fdd\u7cbe\u786e\u5b9a\u91cf\uff0c\u82e5\u663e\u8272\u53cd\u5e94\u65f6\u95f4\u548c\u6e29&nbsp;\u5ea6\u65e0\u6cd5\u7cbe\u786e\u63a7\u5236\uff0c\u5b9c\u6bcf\u6b21\u90fd\u505a\u6807\u51c6\u66f2\u7ebf\u3002<\/p>\n\n\n\n<p>2.\u5728\u8fdb\u884c\u86cb\u767d\u6807\u51c6\u8d2e\u5907\u6db2\u7684\u914d\u5236\u65f6\uff0c\u9700\u786e\u4fdd\u6eb6\u89e3\u5145\u5206\u3002\u7a00\u91ca\u914d\u5236\u86cb\u767d\u6807\u51c6\u5de5\u4f5c\u6db2\u65f6\u5efa\u8bae 10 \u500d\u68af\u5ea6\u7a00\u91ca\uff0c &nbsp;\u4e0d\u8981\u4e00\u6b21\u7a00\u91ca 50 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