{"id":4127,"date":"2023-07-11T14:46:39","date_gmt":"2023-07-11T06:46:39","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=4127"},"modified":"2024-10-24T16:56:49","modified_gmt":"2024-10-24T08:56:49","slug":"%e8%86%9c%e5%86%8d%e7%94%9f%e6%b6%b2-%e9%85%b8%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/4127.html","title":{"rendered":"\u819c\u518d\u751f\u6db2 (\u9178\uff09"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;\"><div class=\"tab_pic\" style=\"width:22%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u518d\u751f\u6db2-scaled-1.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/\u518d\u751f\u6db2-scaled-1.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"\u819c\u518d\u751f\u6db2 (\u9178\uff09\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:73%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">\u819c\u518d\u751f\u6db2 (\u9178\uff09<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/wb\/\">\u86cb\u767d\u8d28\u751f\u7269\u5b66<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a5110\/\u00a5420<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100mL\/500mL<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKW2091<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u74f6<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><td><strong>\u4ea7\u54c1\u540d\u79f0<\/strong><\/td><td><strong>\u4ea7\u54c1\u8d27\u53f7<\/strong><\/td><td><strong>\u4ea7\u54c1\u89c4\u683c<\/strong><\/td><td><strong>\u6709\u6548\u671f<\/strong><\/td><\/tr><tr><td>\u819c\u518d\u751f\u6db2(\u9178)<\/td><td>HKW2091<\/td><td>100ml<\/td><td>\u4e00\u5e74<\/td><\/tr><tr><td>\u819c\u518d\u751f\u6db2(\u9178)<\/td><td>HKW2091<\/td><td>500ml<\/td><td>\u4e00\u5e74<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><br>\u819c\u518d\u751f\u6db2(\u9178) \uff0c\u53ef\u4ee5\u5bf9 western\u00a0\u5b9e\u9a8c\u4e2d\u8f6c\u79fb\u4e86\u86cb\u767d\u7684\u819c\u8fdb\u884c\u6d17\u8131\u540e\u8fdb\u884c\u591a\u6b21 Western Blot\u00a0\u68c0\u6d4b\u3002\u4f8b\u5982\u5728western\u00a0\u4e2d\u5b8c\u6210\u4e86\u4e00\u6297\u4e8c\u6297\u7ed3\u5408\u53ca\u540e\u7eed\u7684\u5316\u5b66\u53d1\u5149\u68c0\u6d4b\u540e\uff0c\u53ef\u901a\u8fc7\u819c\u518d\u751f\u6db2(\u9178)\u00a0\u5904\u7406\u540e\u518d\u5bf9 GAPDH\u3001<br>\u03b2-actin\u00a0\u7b49\u5185\u53c2\u86cb\u767d\u8fdb\u884c\u68c0\u6d4b\u3002\u4f8b\u5982\u63d0\u53d6\u7684\u86cb\u767d\u6837\u54c1\u8f83\u5c11\uff0c\u4e0d\u8db3\u4ee5\u8fdb\u884c\u591a\u00a0\u4e2a\u86cb\u767d\u7684\u68c0\u6d4b\uff0c\u4e5f\u53ef\u4ee5\u901a\u8fc7\u819c\u518d\u751f\u6db2(\u9178) \u5904\u7406\u53bb\u9664\u819c\u4e0a\u7ed3\u5408\u7684\u6297\u4f53\uff0c\u8fdb\u884c\u591a\u6b21\u68c0\u6d4b\u3002\u91c7\u7528\u8fd9\u4e00 \u7b56\u7565\u591a\u6b21\u68c0\u6d4b\u540c\u4e00\u5f20\u819c\u4e0a\u7684\u86cb\u767d\uff0c\u80fd\u7701\u53bb\u86cb\u767d\u7535\u6cf3\u548c\u8f6c\u819c\u7b49\u8017\u8d39\u6027\u6b65\u9aa4\uff0c\u800c\u4e14\u53ef\u4ee5\u6d88\u9664\u91cd\u65b0\u4e0a\u00a0\u6837\u5e26\u6765\u7684\u8bef\u5dee\uff0c\u4f7f\u7ed3\u679c\u53ef\u6bd4\u6027\u66f4\u5f3a\u3002<br>\u672c\u4ea7\u54c1\u4e3b\u8981\u6210\u5206\u4e3a Tris-HCl \u7f13\u51b2\u6db2\uff0cSDS \u548c\u03b2-\u5def\u57fa\u4e59\u9187\uff0c\u5448\u9178\u6027\u3002<br><br><strong>\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/strong><br>\u51b0\u888b (wet\u00a0ice) \u8fd0\u8f93\uff0c4\u2103\u4fdd\u5b58\uff0c\u6709\u6548\u671f12\u4e2a\u6708\u3002<br><br><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><br>1. \u5c06\u9700\u8981\u5904\u7406\u7684\u819c\u7ecf\u84b8\u998f\u6c34\u6f02\u6d17 5 min\uff0c\u7136\u540e\u5145\u5206\u6d78\u6ce1\u4e8e\u819c\u518d\u751f\u6db2(\u9178)\u4e2d\uff0c\u5ba4\u6e29\u6447\u5e8a\u4e0a\u632f\u00a0\u8361 10-60 min\u3002\u53ef\u5728\u6d17\u8131\u4e00\u5b9a\u65f6\u95f4\u540e\uff0c\u5bf9\u819c\u8fdb\u884c ECL \u663e\u8272\u3001\u66dd\u5149\u68c0\u6d4b\u786e\u5b9a\u662f\u5426\u6d17\u8131\u5e72\u51c0\u3002\u5982\u00a0\u672a\u6d17\u8131\u5e72\u51c0\uff0c\u5219\u7ee7\u7eed\u8fdb\u5165\u819c\u518d\u751f\u6db2(\u9178)\u6d78\u6ce1\u5904\u7406\uff0c\u76f4\u5230\u6d17\u8131\u5e72\u51c0\u3002<br>2. \u6d17\u8131\u5e72\u51c0\u540e\uff0c\u53d6\u51fa\u819c\u7528\u00a0TBST \u6f02\u6d17\u4e24\u6b21\uff0c\u76f4\u63a5\u8fdb\u884c\u4e00\u6297\u7684\u5b75\u80b2\u68c0\u6d4b\u3002<br><br><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><br>1. \u00a0\u672c\u8bd5\u5242\u9002\u7528\u4e8e ECL\u00a0\u7b49\u5316\u5b66\u53d1\u5149\u8bd5\u5242\u68c0\u6d4b\u540e\u7684\u819c\uff0c\u975e\u5316\u5b66\u53d1\u5149\u8bd5\u5242\u8fdb\u884c\u7684 western \u68c0\u6d4b\u00a0\u4f8b\u5982 DAB\u3001NBT\/BCIP\u00a0\u7b49\uff0c\u4e0d\u9002\u5b9c\u7528\u672c\u4ea7\u54c1\u3002<br>2. \u00a0\u819c\u7c7b\u578b\u3001\u6297\u4f53\u53ca\u6297\u539f\u7279\u6027\u90fd\u53ef\u80fd\u5f71\u54cd\u6d17\u8131\u7a0b\u5ea6\uff0c\u5efa\u8bae\u8fdb\u884c\u6478\u7d22\u5b9e\u9a8c\u83b7\u5f97\u6700\u4f73\u6761\u4ef6\u3002<br>3. \u00a0\u4f7f\u7528\u8131\u8102\u5976\u7c89\u5c01\u95ed\u7684\u819c\u8981\u6bd4\u4f7f\u7528 BSA\u00a0\u5c01\u95ed\u7684\u819c\u66f4\u5bb9\u6613\u518d\u751f\uff0c\u6545\u63a8\u8350\u91c7\u7528\u8131\u8102\u5976\u7c89\u8fdb\u884c \u5c01\u95ed\u3002<br>4. \u00a0\u4e3a\u83b7\u5f97\u6700\u4f73\u6548\u679c\uff0c\u63a8\u8350\u4f7f\u7528 PVDF \u819c\u3002<br>5. \u00a0\u672c\u4ea7\u54c1\u5728\u4f4e\u6e29\u4e0b\u6613\u51fa\u73b0\u767d\u8272\u7d6e\u72b6\u6c89\u6dc0\uff0c\u5c5e\u6b63\u5e38\u73b0\u8c61\uff0c\u53ef\u7f6e\u4e8e 37\u2103\u590d\u6e29\u540e\u4f7f\u7528\u3002<br>6. \u00a0\u64cd\u4f5c\u65f6\u8bf7\u7a7f\u5b9e\u9a8c\u670d\uff0c\u4f69\u6234\u4e00\u6b21\u6027\u624b\u5957\u3002<\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-9a22675d-b065-4919-84b5-918455fefc2b\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20240919162400259.pdf\">\u819c\u518d\u751f\u6db2(\u9178) HKW2091\u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20240919162400259.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-9a22675d-b065-4919-84b5-918455fefc2b\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div 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