{"id":4087,"date":"2023-07-11T11:26:15","date_gmt":"2023-07-11T03:26:15","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=4087"},"modified":"2024-09-23T15:15:55","modified_gmt":"2024-09-23T07:15:55","slug":"ip%e8%a3%82%e8%a7%a3%e6%b6%b2","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/4087.html","title":{"rendered":"IP\u88c2\u89e3\u6db2"},"content":{"rendered":"\n<figure class=\"wp-block-table\"><table><tbody><tr><td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/hkw2012-1.png?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/hkw2012-1.png?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"IP\u88c2\u89e3\u6db2\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">IP\u88c2\u89e3\u6db2\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/wb\/dbztqydl\/\">\u86cb\u767d\u8d28\u63d0\u53d6\u4e0e\u5b9a\u91cf<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/wb\/\">\u86cb\u767d\u8d28\u751f\u7269\u5b66<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a5160<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100ml<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKW2012<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u74f6<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><td>\u4ea7\u54c1\u76ee\u5f55<\/td><td>\u4e3b\u8981\u6210\u5206<\/td><td>\u4ea7\u54c1\u89c4\u683c<\/td><\/tr><tr><td>IP\u88c2\u89e3\u6db2<\/td><td>NP-40<\/td><td>100mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p style=\"font-size:15px\"><strong>\u4ea7\u54c1\u7b80\u4ecb<\/strong><\/p>\n\n\n\n<p>IP\u88c2\u89e3\u6db2\u662f\u4e00\u79cd\u5728\u975e\u53d8\u6027\u6761\u4ef6\u4e0b\u88c2\u89e3\u7ec6\u80de\u6216\u7ec4\u7ec7\u5236\u5907\u86cb\u767d\u6837\u54c1\u7684\u88c2\u89e3\u6db2\u3002\u7ecf\u672c\u88c2\u89e3\u6db2\u88c2\u89e3\u7ec4\u7ec7\u6216\u7ec6\u80de\u5f97\u5230\u7684\u86cb\u767d\u6837\u672c\uff0c\u53ef\u5e94\u7528\u4e8ePAGE\uff0cwestern blot\uff0c\u514d\u75ab\u6c89\u6dc0(Immunol Precipitation\uff0cIP)\u3001\u514d\u75ab\u5171\u6c89\u6dc0(co-IP)\u3001ChIP\uff08Chromatin Immunopre-cipitation\uff09\u548cELISA\u7b49\u5bf9\u86cb\u767d\u4fdd\u6301\u6d3b\u6027\u6709\u8981\u6c42\u7684\u5b9e\u9a8c\u3002<\/p>\n\n\n\n<p>\u672c\u4ea7\u54c1\u4e3b\u8981\u6210\u5206\u4e3a25 mM Tris-HCl\uff0c150 mM NaCl\uff0c1 mM EDTA\u53ca1% NP-40\u3002\u53ef\u9002\u7528\u4e8e\u52a8\u7269\u6216\u690d\u7269\u7ec4\u7ec7\u53ca\u7ec6\u80de\u6837\u54c1\uff0c\u4e5f\u53ef\u7528\u4e8e\u771f\u83cc\u6216\u7ec6\u83cc\u6837\u54c1\u3002<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><strong>\u50a8\u5b58\u4e0e\u8fd0\u8f93<\/strong><\/p>\n\n\n\n<p>\u51b0\u888b\uff08wet ice\uff09\u8fd0\u8f93\uff1b4\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u6709\u6548\u671f12\u4e2a\u6708\u3002<br><strong>\u672c\u4ea7\u54c1\u4ec5\u4f9b\u79d1\u7814\u7528\u9014\uff0c\u4e0d\u7528\u4e8e\u4e34\u5e8a\u8bca\u65ad\uff01<\/strong><\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-e30e1c17-2845-4214-87c3-11af8b8e6a7c\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20240918092646732.pdf\">IP\u88c2\u89e3\u6db2 HKW2012 \u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20240918092646732.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-e30e1c17-2845-4214-87c3-11af8b8e6a7c\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div 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