{"id":3769,"date":"2023-07-05T11:05:27","date_gmt":"2023-07-05T03:05:27","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=3769"},"modified":"2024-09-23T15:46:31","modified_gmt":"2024-09-23T07:46:31","slug":"iftunel%e5%8f%8c%e6%9f%93","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/3769.html","title":{"rendered":"IF+TUNEL\u53cc\u67d3"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20240910115227133.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20240910115227133.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"IF+TUNEL\u53cc\u67d3\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">IF+TUNEL\u53cc\u67d3<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/fzblfw\/hktunel\/\">Tunel<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/fzblfw\/ygdsfw\/\">\u514d\u75ab\u8367\u5149<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/fzblfw\/\">\u5206\u5b50\u75c5\u7406<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/gallery\/\">\u56fe\u5e93<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/\">\u5b9e\u9a8c\u670d\u52a1<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe5200<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u5f20<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKF2039<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\"><\/span><span style=\"color:#666666;\"><\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">\u6d69\u514b<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p>\u3010\u4ea7\u54c1\u4fe1\u606f\u3011<\/p>\n\n\n\n<p><strong>\u77f3\u8721\u5207\u7247\u8367\u5149tunel+\u514d\u75ab\u8367\u5149\u53cc\u6807\u5b9e\u9a8c\u6b65\u9aa4<\/strong><br><strong>1\u3001\u00a0\u77f3\u8721\u5207\u7247\u8131\u8721\u81f3\u6c34\uff1a<\/strong>\u4f9d\u6b21\u5c06\u5207\u7247\u653e\u5165\u4e8c\u7532\u82ef\u216012min-\u4e8c\u7532\u82ef\u216112min-\u65e0\u6c34\u4e59\u9187\u21606min-95%\u9152\u7cbe6min-85%\u9152\u7cbe6min-\u84b8\u998f\u6c34\u6d17\uff08\u51ac\u5929\u8131\u8721\u65f6\u95f4\u7a0d\u5fae\u5ef6\u957f\uff09\u3002<br><strong>2\u3001\u00a0\u4fee\u590d\uff1a<\/strong>\u7ec4\u7ec7\u5207\u7247\u7f6e\u4e8e\u76db\u6ee1EDTA\u4fee\u590d\u6db2\uff08PH8.0\uff09\u7684\u4fee\u590d\u76d2\u4e2d\u4e8e\u5fae\u6ce2\u7089\u5185\u8fdb\u884c\u6297\u539f\u4fee\u590d\uff0c\u4e2d\u706b8min\u505c\u706b8min<br>\u4e2d\u4f4e\u706b7min\uff0c\u6b64\u8fc7\u7a0b\u4e2d\u5e94\u9632\u6b62\u7f13\u51b2\u6db2\u8fc7\u5ea6\u84b8\u53d1\uff0c\u5207\u52ff\u5e72\u7247\u3002\u81ea\u7136\u51b7\u5374\u540e\u5c06\u73bb\u7247\u7f6e\u4e8ePBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u3002<br><strong>3\u3001\u00a0\u963b\u65ad\u5185\u6e90\u6027\u8fc7\u6c27\u5316\u7269\u9176\uff1a<\/strong>\u5207\u7247\u52a0\u4e0a3%\u7684\u53cc\u6c27\u6c34\uff0c\u5ba4\u6e29\u5b75\u80b225min\uff0c\u5c06\u73bb\u7247\u7f6ePBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u3002<br><strong>4\u3001\u00a0\u8840\u6e05\u5c01\u95ed:<\/strong>\u5207\u7247\u7a0d\u7529\u5e72\u540e\u7528\u7ec4\u5316\u7b14\u5728\u7ec4\u7ec7\u5468\u56f4\u753b\u5708\uff08\u9632\u6b62\u6297\u4f53\u6d41\u8d70\uff09\uff0c\u5728\u7ec4\u5316\u5708\u5185\u6ef4\u52a03%BSA\u00a0\u5747\u5300\u8986\u76d6\u7ec4\u7ec7\uff0c\u5ba4\u6e29\u5c01\u95ed30min\u3002<br><strong>5\u3001\u00a0\u52a0\u4e00\u6297\uff1a<\/strong>\u5207\u7247\u7a0d\u7529\u5e72\u540e\u7528\u5728\u5708\u5185\u6ef4\u52a0\u6309\u4e00\u5b9a\u6bd4\u4f8b\u7a00\u7684\u6297\u4f53\u8986\u76d6\u7ec4\u7ec7\u3002\u5207\u7247\u5e73\u653e\u4e8e\u6e7f\u76d2\u51854\u00b0C\u5b75\u80b2\u8fc7\u591c\u3002<br><strong>6\u3001\u00a0\u52a0\u4e8c\u6297\uff1a<\/strong>\u73bb\u7247\u7f6e\u4e8ePBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u3002\u5207\u7247\u7a0d\u7529\u5e72\u540e\u5728\u5708\u5185\u6ef4\u52a0\u4e0e\u4e00\u6297\u76f8\u5e94\u79cd\u5c5e\u7684\u4e8c\u6297\u8986\u76d6\u7ec4\u7ec7\uff0c\u907f\u5149\u5ba4\u6e29\u5b75\u80b250min\u3002<br><strong>7\u3001\u00a0\u4fe1\u53f7\u653e\u5927:<\/strong>\u5c06\u73bb\u7247\u7f6e\u4e8ePBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u00a0\u3002\u6ef4\u52a0\u76f8\u5bf9\u5e94\u989c\u8272Flare\u4fe1\u53f7\u653e\u5927\u8bd5\u5242\uff0c3-5min.\u5c06\u73bb\u7247\u7f6e\u4e8ePBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u00a0\u3002<br><strong>8\u3001\u00a0\u4fee\u590d\uff1a<\/strong>\u7ec4\u7ec7\u5207\u7247\u7f6e\u4e8e\u76db\u6ee1EDTA\u4fee\u590d\u6db2\uff08PH8.0\uff09\u7684\u4fee\u590d\u76d2\u4e2d\u4e8e\u5fae\u6ce2\u7089\u5185\u8fdb\u884c\u6297\u539f\u4fee\u590d\uff0c\u4e2d\u706b8min\u505c\u706b8min\u4e2d\u4f4e\u706b7min\uff0c\u6b64\u8fc7\u7a0b\u4e2d\u5e94\u9632\u6b62\u7f13\u51b2\u6db2\u8fc7\u5ea6\u84b8\u53d1\uff0c\u5207\u52ff\u5e72\u7247\u3002\u81ea\u7136\u51b7\u5374\u540e\u5c06\u73bb\u7247\u7f6e\u4e8ePBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u3002<br><strong>9\u3001\u00a0\u52a0\u8bd5\u5242tunel\u5de5\u4f5c\u6db2\uff1a<\/strong>\u6309\u7247\u5b50\u6570\u91cf\u548c\u7ec4\u7ec7\u5927\u5c0f\u53d6tunel\u8bd5\u5242\u76d2\u5185\u9002\u91cf\u8bd5\u5242TdT\u9176\u548c\u53cd\u5e94\u6db2\u8bd5\u52421:50\u6df7\u5408\uff0c\u52a0\u5230\u5708\u5185\u8986\u76d6\u7ec4\u7ec7\uff0c\u5207\u7247\u5e73\u653e\u4e8e\u6e7f\u76d2\u5185\uff0c37\u2103\u6052\u6e29\u7bb1\u5b75\u80b21.5\u5c0f\u65f6\uff0c\u6e7f\u76d2\u5185\u52a0\u5c11\u91cf\u6c34\u4fdd\u6301\u6e7f\u5ea6\u3002<br><strong>10\u3001DAPI\u590d\u67d3\u7ec6\u80de\u6838\uff1a<\/strong>\u5207\u7247\u7528PBS\uff08PH7.4\uff09\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u3002\u53bb\u9664PBS\u540e\u5728\u5708\u5185\u6ef4\u52a0DAPI\u67d3\u6db2\uff0c\u907f\u5149\u5ba4\u6e29\u5b75\u80b2<br>10min\u3002<br><strong>11\u3001\u5c01\u7247\uff1a<\/strong>\u73bb\u7247\u7f6e\u4e8ePBS\uff08PH7.4\uff09\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u3002\u5207\u7247\u7a0d\u7529\u5e72\u540e\u7528\u6297\u8367\u5149\u6dec\u706d\u5c01\u7247\u5242\u5c01\u7247\u3002<br><strong>12\u3001 \u955c\u68c0\u62cd\u7167\uff1a<\/strong>\u5207\u7247\u4e8e\u5c3c\u5eb7\u5012\u7f6e\u8367\u5149\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u5e76\u91c7\u96c6\u56fe\u50cf\u3002\uff08\u7d2b\u5916\u6fc0\u53d1\u6ce2\u957f330-380nm\uff0c\u53d1\u5c04\u6ce2\u957f420nm\uff1b<br>FITC\u7eff\u5149\u6fc0\u53d1\u6ce2\u957f465-495nm\uff0c\u53d1\u5c04\u6ce2\u957f515-555 nm\uff1bCY3\u7ea2\u5149\u6fc0\u53d1\u6ce2\u957f510-560\uff0c\u53d1\u5c04\u6ce2\u957f590nm\uff09<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td>\u4ea7\u54c1\u540d\u79f0<\/td><td>\u4ea7\u54c1\u8d27\u53f7<\/td><td>\u89c4\u683c<\/td><td>\u4ef7\u683c<\/td><\/tr><tr><td>IF+TUNEL\u53cc\u67d3<\/td><td>HKF2039<\/td><td>\u5f20<\/td><td>200\u5143<\/td><\/tr><\/tbody><\/table><figcaption 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