{"id":3712,"date":"2023-07-04T09:30:59","date_gmt":"2023-07-04T01:30:59","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=3712"},"modified":"2024-11-27T11:24:22","modified_gmt":"2024-11-27T03:24:22","slug":"af488%e6%a0%87%e8%ae%b0%e7%9a%84-%e5%b1%b1%e7%be%8a%e6%8a%97%e5%b0%8f%e9%bc%a0igg","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/3712.html","title":{"rendered":"AF488\u6807\u8bb0\u7684\u5c71\u7f8a\u6297\u5c0f\u9f20IgG"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"AF488\u6807\u8bb0\u7684\u5c71\u7f8a\u6297\u5c0f\u9f20IgG\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">AF488\u6807\u8bb0\u7684\u5c71\u7f8a\u6297\u5c0f\u9f20IgG<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/kt\/ek\/\">\u4e8c\u6297<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/kt\/\">\u6297\u4f53<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe5280<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100ul<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKS25301<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u652f<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><th><span style=\"font-size: 24px; color: #333333;\"><strong>\u54c1\u724c<\/strong><\/span><\/th><th><span style=\"font-size: 24px; color: #333333;\"><strong>\u5546\u54c1\u7f16\u7801<\/strong><\/span><\/th><th><span style=\"font-size: 24px; color: #333333;\"><strong>\u5546\u54c1\u540d\u79f0<\/strong><\/span><\/th><th><span style=\"font-size: 24px; color: #333333;\"><strong>\u89c4\u683c<\/strong><\/span><\/th><th><span style=\"font-size: 24px; color: #333333;\"><strong>\u5355\u4f4d<\/strong><\/span><\/th><th><span style=\"font-size: 24px; color: #333333;\"><strong>\u4ef7\u683c<\/strong><\/span><\/th><\/tr><tr><th><span style=\"color: #333333;\">HaoKebio<\/span><\/th><th>HKS25301<\/th><th>AF488\u6807\u8bb0\u7684\u5c71\u7f8a\u6297\u5c0f\u9f20IgG<\/th><th>100ul<\/th><th>\u652f<\/th><th>\uffe5280<\/th><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><br>      \u672c\u54c1\u662f\u7531 Alexa Fluor 488 \u6807\u8bb0\u7684\u5c71\u7f8a\u6297\u5c0f\u9f20 IgG\uff08H+L\uff09\uff0c\u4f7f\u7528\u6297\u539f\u5076\u8054\u7684\u743c\u8102\u7cd6\u5fae\u73e0\u4ece\u5c71\u7f8a\u6297\u8840\u6e05\u5185 \u4eb2\u548c\u8272\u8c31\u7eaf\u5316\u6240\u5f97\u3002\u514d\u75ab\u7535\u6cf3\u548c\/\u6216 ELISA \u6cd5\u68c0\u6d4b\u663e\u793a\u672c\u54c1\u7279\u5f02\u6027\u7ed3\u5408\u5b8c\u6574\u7684\u5c0f\u9f20 IgG \u5206\u5b50\uff0c\u4e5f\u4f1a\u4e0e\u5176\u4ed6 \u5c0f\u9f20\u514d\u75ab\u7403\u86cb\u767d\u7684\u8f7b\u94fe\u7ed3\u5408\u3002\u53ef\u80fd\u4f1a\u4e0e\u5176\u4ed6\u7269\u79cd\u514d\u75ab\u7403\u86cb\u767d\u53d1\u751f\u4ea4\u53c9\u53cd\u5e94\uff0c\u4f46\u4e0d\u4f1a\u8bc6\u522b\u975e\u514d\u75ab\u7403\u86cb\u767d\u7c7b\u7684 \u8840\u6e05\u86cb\u767d\u3002Alexa Fluor 488 \u7684\u5149\u8c31\u6027\u8d28\u540c FITC\uff0cAmax\uff08\u6700\u5927\u6fc0\u53d1\u6ce2\u957f\uff09\u4e3a 493 nm\uff0cEmax\uff08\u6700\u5927\u53d1\u5c04\u6ce2\u957f\uff09\u4e3a 519 nm\u3002<br>      \u672c\u54c1\u9002\u5408\u505a\u5355\u6807\u5b9e\u9a8c\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u591a\u79cd\u514d\u75ab\u5b66\u5b9e\u9a8c\u5982\u514d\u75ab\u7ec6\u80de\u5316\u5b66\uff08ICC\uff09\uff0c\u6d41\u5f0f\u7ec6\u80de\u672f\uff08FC\uff09\u4ee5\u53ca\u514d \u75ab\u7ec4\u5316\uff08IHC\uff09\u7b49\u3002\u8981\u505a\u591a\u6807\uff08multiple-labeling\uff09\u5b9e\u9a8c\uff0c\u5efa\u8bae\u4f7f\u7528\u4e0e\u76f8\u8fd1\u7269\u79cd\u7684\u8840\u6e05\u86cb\u767d\u6216\u8005\u514d\u75ab\u7403\u86cb\u767d\u9884 \u5148\u7ecf\u8fc7\u4eb2\u548c\u5438\u9644\u5904\u7406\u7684\u4e8c\u6297\u3002<br><strong>\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/strong><br>\u51b0\u888b\uff08wet ice\uff09\u8fd0\u8f93\uff1b-20\u2103\u4fdd\u5b58\uff0c\u5c3d\u91cf\u907f\u514d\u53cd\u590d\u51bb\u878d\uff0c\u6709\u6548\u671f\u4e00\u5e74\u3002<br><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><br>\u5efa\u8bae\u7a00\u91ca\u6d53\u5ea6\uff1a1:50-1:400\uff08For most application\uff09\u3002<br><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><br>1.\u672c\u54c1\u542b\u53e0\u6c2e\u5316\u94a0\uff0c\u5bf9\u4eba\u4f53\u6709\u5bb3\uff0c\u8bf7\u6ce8\u610f\u9002\u5f53\u9632\u62a4\u3002<br>2.\u63a8\u8350\u4f7f\u75285%\u7684\u9a74\uff08\u4e8c\u6297\u5bbf\u4e3b\uff09\u6b63\u5e38\u8840\u6e05\u8fdb\u884c\u5c01\u95ed\u3002BSA \u6216\u8005\u51bb\u5e72\u5976\u7c89\u542b\u6709 IgG\uff0c\u68c0\u6d4b\u725b\u3001\u9a6c\u3001\u5c71\u7f8a\u3001\u7ef5\u7f8a\u7684\u4e8c\u6297\u80fd\u8ddf\u725b IgG \u53d1\u751f\u53cd\u5e94\uff0c\u56e0\u6b64\uff0c\u7528 BSA \u6216\u8005\u725b\u5976\u5c01\u95ed\u6216\u4f5c\u4e3a\u6297\u4f53\u7a00\u91ca\u6db2\u6709\u53ef\u80fd\u589e\u5927\u53cd\u5e94\u80cc\u666f\u6216\u964d\u4f4e\u6297\u4f53\u6548\u4ef7\u3002<br>3.\u4e3a\u4e86\u60a8\u7684\u5b89\u5168\u548c\u5065\u5eb7\uff0c\u8bf7\u7a7f\u5b9e\u9a8c\u670d\u5e76\u6234\u4e00\u6b21\u6027\u624b\u5957\u64cd\u4f5c\u3002<br>4.\u672c\u4ea7\u54c1\u4ec5\u4f5c\u79d1\u7814\u7528\u9014\u3002<\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-be08cd07-17e9-4b59-a0f8-2a3c300da31d\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20241127112312983.pdf\">AF488\u6807\u8bb0\u7684\u5c71\u7f8a\u6297\u5c0f\u9f20IgG HKS25301 \u8bf4\u660e\u4e66<\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/07\/20241127112312983.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-be08cd07-17e9-4b59-a0f8-2a3c300da31d\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":7870,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[102,99,98],"tags":[],"class_list":["post-3712","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-ek","category-kt","category-products"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:6:\"\uffe5280\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u89c4\u683c\";s:4:\"text\";s:5:\"100ul\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:2;a:5:{s:5:\"title\";s:6:\"\u8d27\u53f7\";s:4:\"text\";s:8:\"HKS25301\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:3;a:5:{s:5:\"title\";s:6:\"\u5355\u4f4d\";s:4:\"text\";s:3:\"\u652f\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";b:0;}i:4;a:5:{s:5:\"title\";s:6:\"\u54c1\u724c\";s:4:\"text\";s:8:\"HaoKebio\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}}"],"catce":["sidebar-widgets4"],"views":["1054"],"_dp_original":["3710"],"_edit_lock":["1732677862:2"],"_edit_last":["2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/3712"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=3712"}],"version-history":[{"count":8,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/3712\/revisions"}],"predecessor-version":[{"id":13003,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/3712\/revisions\/13003"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=3712"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=3712"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=3712"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}