{"id":3511,"date":"2023-06-12T17:02:39","date_gmt":"2023-06-12T09:02:39","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=3511"},"modified":"2024-10-10T17:04:27","modified_gmt":"2024-10-10T09:04:27","slug":"pbs%e7%bc%93%e5%86%b2%e6%b6%b2%ef%bc%88%e5%b9%b2%e7%b2%89%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/3511.html","title":{"rendered":"PBS\u7f13\u51b2\u6db2\uff08\u5e72\u7c89\uff09"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;\"><div class=\"tab_pic\" style=\"width:30%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"PBS\u7f13\u51b2\u6db2\uff08\u5e72\u7c89\uff09\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:65%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">PBS\u7f13\u51b2\u6db2\uff08\u5e72\u7c89\uff09<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/uncategorized\/\">\u672a\u5206\u7c7b<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe55<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HK0002<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">2L<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u5305<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p><\/p>\n\n\n\n<p>PBS&nbsp;\u7f13\u51b2\u6db2&nbsp;(\u5e72\u7c89)<\/p>\n\n\n\n<p>Phosphate&nbsp;Buffered&nbsp;Saline&nbsp;(PBS\uff0cPowder)&nbsp;\u8d27\u53f7\uff1aHK0002<\/p>\n\n\n\n<p>\u3010\u4ea7\u54c1\u4fe1\u606f\u3011<\/p>\n\n\n\n<figure class=\"wp-block-table is-style-stripes\"><table><tbody><tr><td>\u4ea7\u54c1\u540d\u79f0<\/td><td>\u4ea7\u54c1\u8d27\u53f7<\/td><td>\u89c4\u683c<\/td><td>\u6709\u6548\u671f<\/td><\/tr><tr><td>PBS&nbsp;\u7f13\u51b2\u6db2 (\u5e72\u7c89)<\/td><td>HK0002<\/td><td>2L<\/td><td>24 \u4e2a\u6708<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p><strong>\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><\/p>\n\n\n\n<p>\u672c\u4ea7\u54c1 PBS&nbsp;\u7f13\u51b2\u6db2 (\u5e72\u7c89) \uff0c\u5373 Phosphate&nbsp;Buffered&nbsp;Saline&nbsp;(PBS\uff0cPowder) \uff0c\u6bcf\u5305&nbsp;\u53ef\u914d\u5236\u6210 2 L&nbsp;PBS&nbsp;\u7f13\u51b2\u6db2\u3002\u672c\u4ea7\u54c1\u7c89\u672b\u7ec6\u817b\uff0c\u6eb6\u89e3\u8fc5\u901f\uff0c\u65b9\u4fbf\u4f7f\u7528\u3002\u914d\u5236\u6210\u6eb6\u6db2\u540e\u53ef\u5e94\u7528&nbsp;\u4e8e ELISA\u3001Western&nbsp;Blot&nbsp;(WB) \u514d\u75ab\u5370\u8ff9\u3001\u514d\u75ab\u7ec4\u5316\u3001\u514d\u75ab\u8367\u5149\u7b49\u514d\u75ab\u76f8\u5173\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u7b49\u6240&nbsp;\u9700\u7684\u6f02\u6d17\u4ee5\u53ca\u5c01\u95ed\u6db2\u914d\u5236\u7b49\uff0c\u53ef\u6839\u636e\u5177\u4f53\u5b9e\u9a8c\u8981\u6c42\u52a0\u5165\u6240\u9700\u6d53\u5ea6\u7684\u53bb\u57a2\u5242(\u5982\u5410\u6e29 20 \u7b49)\u3002&nbsp;\u78b1\u6027\u78f7\u9178\u9176 AP&nbsp;\u6807\u8bb0\u4f53\u7cfb\u76f8\u5173\u5b9e\u9a8c\uff0c\u8bf7\u9009\u62e9 TBS&nbsp;\u7f13\u51b2\u6db2\uff0c\u907f\u514d PBS&nbsp;\u5bf9\u78b1\u6027\u78f7\u9178\u9176\u7684\u5f71\u54cd\u3002&nbsp;\u672c\u4ea7\u54c1\u4e3b\u8981\u7ec4\u5206\u4e3a 10 mM&nbsp;\u78f7\u9178\u94a0\uff0c150 mM&nbsp;NaCl\uff0cpH7.2-7.4@25\u2103\u3002<\/p>\n\n\n\n<p><strong>\u3010\u50a8\u5b58\u4e0e\u8fd0\u8f93\u3011<\/strong><\/p>\n\n\n\n<p>\u5e38\u6e29\u8fd0\u8f93\uff1b\u9634\u51c9\u5e72\u71e5\u5904\u4fdd\u5b58\uff0c\u6709\u6548\u671f 24 \u4e2a\u6708\u3002<\/p>\n\n\n\n<p><strong>\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><\/p>\n\n\n\n<p>\u6bcf\u888b PBS&nbsp;\u7f13\u51b2\u6db2 (\u5e72\u7c89) \u5f7b\u5e95\u6eb6\u89e3\u4e8e 2000 mL&nbsp;\u6c34\u4e2d\u3002<\/p>\n\n\n\n<p><strong>\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><\/p>\n\n\n\n<p>1.&nbsp;\u6eb6\u89e3\u540e\u8bf7\u5c3d\u5feb\u4f7f\u7528\u3002<\/p>\n\n\n\n<p>2. \u672c\u4ea7\u54c1\u4e0d\u542b\u94be\u79bb\u5b50\u53ca\u9541\u79bb\u5b50\u3002<\/p>\n\n\n\n<p>3. \u64cd\u4f5c\u65f6\u8bf7\u7a7f\u5b9e\u9a8c\u670d\uff0c\u4f69\u6234\u4e00\u6b21\u6027\u624b\u5957\u3002<\/p>\n\n\n\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-3a846072-d11b-45c3-8fe7-aaa64b189231\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/06\/20240919154536612.pdf\">PBS \u7f13\u51b2\u6db2\uff08\u5e72\u7c89\uff09 HK0002 \u8bf4\u660e\u4e66 <\/a><a href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2023\/06\/20240919154536612.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-3a846072-d11b-45c3-8fe7-aaa64b189231\">\u4e0b\u8f7d<\/a><\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p>\u65e0<\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":7870,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-3511","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"catce":["sidebar-widgets4"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:4:\"\uffe55\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u8d27\u53f7\";s:4:\"text\";s:6:\"HK0002\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:2;a:5:{s:5:\"title\";s:6:\"\u89c4\u683c\";s:4:\"text\";s:2:\"2L\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:3;a:5:{s:5:\"title\";s:6:\"\u5355\u4f4d\";s:4:\"text\";s:3:\"\u5305\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";b:0;}i:4;a:5:{s:5:\"title\";s:6:\"\u54c1\u724c\";s:4:\"text\";s:8:\"HaoKebio\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}}"],"views":["972"],"_dp_original":["3509"],"_edit_lock":["1729759329:2"],"_edit_last":["2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/3511"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=3511"}],"version-history":[{"count":4,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/3511\/revisions"}],"predecessor-version":[{"id":10832,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/3511\/revisions\/10832"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=3511"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=3511"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=3511"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}