{"id":13683,"date":"2024-12-04T14:09:12","date_gmt":"2024-12-04T06:09:12","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=13683"},"modified":"2024-12-16T10:53:44","modified_gmt":"2024-12-16T02:53:44","slug":"%e5%8e%9f%e4%bd%8d%e6%9d%82%e4%ba%a4%ef%bc%88dab%e6%98%be%e8%89%b2%ef%bc%89","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/13683.html","title":{"rendered":"\u539f\u4f4d\u6742\u4ea4\uff08DAB\u663e\u8272\uff09"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"\u539f\u4f4d\u6742\u4ea4\uff08DAB\u663e\u8272\uff09\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">\u539f\u4f4d\u6742\u4ea4\uff08DAB\u663e\u8272\uff09\n\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/\">\u5b9e\u9a8c\u670d\u52a1<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe5240<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">\u5f20<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKF5031<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\"><\/span><span style=\"color:#666666;\"><\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">\u6d69\u514b<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p>\u3000\u3000<strong>\u9879\u76ee\u4ecb\u7ecd\uff1a<\/strong><\/p>\n\n\n\n<p>\u3000\u30001.\u539f\u4f4d\u6742\u4ea4\u539f\u7406\uff1a\u539f\u4f4d\u6742\u4ea4\u662f\u6307\u5c06\u7279\u5b9a\u6807\u8bb0\u7684\u5df2\u77e5\u987a\u5e8f\u6838\u9178\u4e3a\u63a2\u9488\u4e0e\u7ec6\u80de\u6216\u7ec4\u7ec7\u5207\u7247\u4e2d\u6838\u9178\u8fdb\u884c\u6742\u4ea4\uff0c\u4ece\u800c\u5bf9\u7279\u5b9a\u6838\u9178\u987a\u5e8f\u8fdb\u884c\u7cbe\u786e\u5b9a\u91cf\u5b9a\u4f4d\u7684\u8fc7\u7a0b\u3002\u539f\u4f4d\u6742\u4ea4\u53ef\u4ee5\u5728\u7ec6\u80de\u6807\u672c\u6216\u7ec4\u7ec7\u6807\u672c\u4e0a\u8fdb\u884c\u3002<\/p>\n\n\n\n<p>\u3000\u30002.\u6240\u9700\u5668\u6750\uff1a<\/p>\n\n\n\n<p>\u3000\u3000\u6052\u6e29\u7bb1\uff0c\u9ad8\u538b\u706d\u83cc\u9505\uff0c\u79fb\u6db2\u67aa\uff0c\u949f\u6446\u6447\u5e8a\u3002<\/p>\n\n\n\n<p>\u3000\u30003.\u4e3b\u8981\u8bd5\u5242\uff1aDEPC\u3002\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u3002PBS\u7f13\u51b2\u6db2\u300220\u00d7SSC\u6d17\u8131\u6db2\u3002\u86cb\u767d\u9176K\u3002\u82cf\u6728\u7d20\u67d3\u6db2\u3002\u4e2d\u6027\u6811\u80f6\u3002\u6742\u4ea4\u6db2\u3002HRP\u4e8c\u6297\u3002DAB\u663e\u8272\u5242\u3002<\/p>\n\n\n\n<p>\u3000\u3000<strong>\u5b9e\u9a8c\u6b65\u9aa4\uff1a<\/strong><\/p>\n\n\n\n<p>\u3000\u3000\u77f3\u8721\u5207\u7247DAB\u663e\u8272\u539f\u4f4d\u6742\u4ea4\u5b9e\u9a8c\u6b65\u9aa4<\/p>\n\n\n\n<p>\u3000\u30001\u3001\u7ec4\u7ec7\u56fa\u5b9a\uff1a\u7ec4\u7ec7\u53d6\u51fa\u6d17\u51c0\u540e\u7acb\u5373\u653e\u5165\u56fa\u5b9a\u6db2(DEPC\u6c34\u914d\u5236)\u4e2d\u56fa\u5b9a2-12h\u3002<\/p>\n\n\n\n<p>\u3000\u30002\u3001\u8131\u6c34\uff1a\u7ec4\u7ec7\u56fa\u5b9a\u5b8c\u6210\u540e\u7ecf\u68af\u5ea6\u9152\u7cbe\u8131\u6c34\u540e\u6d78\u8721\uff0c\u5305\u57cb\u3002<\/p>\n\n\n\n<p>\u3000\u30003\u3001\u5207\u7247\uff1a\u77f3\u8721\u7ecf\u5207\u7247\u673a\u5207\u7247\uff0c\u644a\u7247\u673a\u635e\u7247\uff0c62\u00b0\u70e4\u7bb1\u70e4\u72472h\u3002<\/p>\n\n\n\n<p>\u3000\u30004\u3001\u77f3\u8721\u5207\u7247\u8131\u8721\u81f3\u6c34\uff1a\u4f9d\u6b21\u5c06\u5207\u7247\u653e\u5165\u4e8c\u7532\u82ef\u216015min-\u4e8c\u7532\u82ef\u216115min-\u65e0\u6c34\u4e59\u9187\u21605min-\u65e0\u6c34\u4e59\u9187\u21615min-85%\u9152\u7cbe5min-75%\u9152\u7cbe5min-DEPC\u6c34\u6d17\u3002<\/p>\n\n\n\n<p>\u3000\u30005\u3001\u6d88\u5316\uff1a\u6839\u636e\u7ec4\u7ec7\u56fa\u5b9a\u65f6\u95f4\u957f\u77ed\uff0c\u5207\u7247\u4e8e\u4fee\u590d\u6db2\u4e2d\u716e\u6cb810-15\u5206\u949f\uff0c\u81ea\u7136\u51b7\u5374\u3002\u540e\u57fa\u56e0\u7b14\u753b\u5708\uff0c\u6839\u636e\u4e0d\u540c\u7ec4\u7ec7\u4e0d\u540c\u6307\u6807\u7279\u6027\uff0c\u6ef4\u52a0\u86cb\u767d\u9176K(20ug\/ml) 37\u00b0\u6d88\u5316min\u3002\u7eaf\u6c34\u51b2\u6d17\u540ePBS\u6d173\u6b21\u00d75min\u3002<\/p>\n\n\n\n<p>\u3000\u30006\u3001\u963b\u65ad\u5185\u6e90\u6027\u8fc7\u6c27\u5316\u7269\u9176\uff1a\u6ef4\u52a03%\u7532\u9187-H2O2\uff0c\u5ba4\u6e29\u907f\u5149\u5b75\u80b215min\uff0c\u5c06\u73bb\u7247\u7f6e\u4e8ePBS(PH7.4)\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u3002<\/p>\n\n\n\n<p>\u3000\u30007\u3001\u9884\u6742\u4ea4\uff1a\u6ef4\u52a0\u9884\u6742\u4ea4\u6db2\uff0c37\u00b0C\u5b75\u80b21h\u3002<\/p>\n\n\n\n<p>\u3000\u30008\u3001\u6742\u4ea4\uff1a\u503e\u53bb\u9884\u6742\u4ea4\u6db2\uff0c\u6ef4\u52a0\u542b\u63a2\u9488\u6742\u4ea4\u6db2, \u6d53\u5ea6\uff0c\u6052\u6e29\u7bb1\u5ea6\u6742\u4ea4\u8fc7\u591c\u3002<\/p>\n\n\n\n<p>\u3000\u30009\u3001\u6742\u4ea4\u540e\u6d17\u6da4\uff1a\u6d17\u53bb\u6742\u4ea4\u6db2\uff0c2\u00d7SSC\uff0c37\u00b0C \u6d1710min\uff0c1\u00d7SSC\uff0c37\u00b0C\u6d172\u00d75min\uff0c0.5\u00d7SSC\u5ba4\u6e29\u6d1710min\u3002\u82e5\u975e\u7279\u5f02\u6742\u4ea4\u4f53\u8f83\u591a\uff0c\u53ef\u4ee5\u589e\u52a0\u7532\u9170\u80fa\u6d17\u6da4\u3002<\/p>\n\n\n\n<p>\u3000\u300010\u3001\u6ef4\u52a0\u5c01\u95ed\u6db2\uff1a\u6ef4\u52a0\u5c01\u95ed\u8840\u6e05BSA \u3002\u5ba4\u6e2930min\u3002<\/p>\n\n\n\n<p>\u3000\u300011\u3001\u6ef4\u52a0\u9f20\u6297\u5730\u9ad8\u8f9b\u6807\u8bb0\u8fc7\u6c27\u5316\u7269\u9176(anti-DIG-HRP)\uff1a\u503e\u53bb\u5c01\u95ed\u6db2\uff0c\u6ef4\u52a0anti-DIG-HRP\u300237\u00b0C\u5b75\u80b240min\uff0c\u540ePBS\u6d174\u6b21\u00d75min\u3002<\/p>\n\n\n\n<p>\u3000\u300012\u3001DAB\u663e\u8272\uff1a\u5207\u7247\u7a0d\u7529\u5e72\u540e\uff0c\u5728\u5708\u5185\u6ef4\u52a0\u65b0\u9c9c\u914d\u5236\u7684DAB\u663e\u8272\u6db2\uff0c\u663e\u5fae\u955c\u4e0b\u63a7\u5236\u663e\u8272\u65f6\u95f4\uff0c\u9633\u6027\u4e3a\u68d5\u9ec4\u8272\uff0c\u7eaf\u6c34\u51b2\u6d17\u5207\u7247\u7ec8\u6b62\u663e\u8272\u3002<\/p>\n\n\n\n<p>\u3000\u300013\uff1a\u590d\u67d3\u7ec6\u80de\u6838\uff1aHarris\u82cf\u6728\u7d20\u590d\u67d33min\u5de6\u53f3\uff0c\u81ea\u6765\u6c34\u6d17\uff0c1%\u76d0\u9178\u9152\u7cbe\u5206\u5316\u6570\u79d2\uff0c\u81ea\u6765\u6c34\u6d17\uff0c\u6c28\u6c34\u8fd4\u84dd\uff0c\u6d41\u6c34\u51b2\u6d17\u3002<\/p>\n\n\n\n<p>\u3000\u300014\uff1a\u8131\u6c34\u5c01\u7247\uff1a\u5c06\u5207\u7247\u4f9d\u6b21\u653e\u516575%\u9152\u7cbe6min-85%\u9152\u7cbe6min\u2014100%\u9152\u7cbeI 6min\u2014100%\u9152\u7cbeII 6min-\u6b63\u4e01\u91876min\u2014\u4e8c\u7532\u82ef\u900f\u660e\uff0c\u5c06\u5207\u7247\u4ece\u4e8c\u7532\u82ef\u4e2d\u62ff\u51fa\u7a0d\u667e\u5e72\u540e\uff0c\u4e2d\u6027\u6811\u80f6\u5c01\u7247\u3002<\/p>\n\n\n\n<p>\u3000\u300015\u3001\u663e\u5fae\u955c\u68c0\uff0c\u56fe\u50cf\u91c7\u96c6\u5206\u6790\u3002<\/p>\n\n\n\n<p>\u3000\u3000<strong>\u7ed3\u679c\u5224\u8bfb\uff1a<\/strong>\u9633\u6027\u4e3a\u68d5\u9ec4\u8272\uff0c\u7ec6\u80de\u6838\u4e3a\u84dd\u8272\u3002<\/p>\n\n\n\n<p>\u3000\u3000<strong>\u6ce8\u610f\u4e8b\u9879\uff1a<\/strong>mRNA\u4e3a\u68c0\u6d4b\u5bf9\u8c61\u65f6\uff0c\u9700\u4e25\u683c\u8981\u6c42\u5b9e\u9a8c\u73af\u5883\u7ecf\u65e0RNA\u9176\u5904\u7406\u3002<\/p>\n\n\n\n<p><br><br><br><\/p>\n\n\n\n<p><br><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ea7\u54c1\u540d\u79f0<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ea7\u54c1\u8d27\u53f7<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ea7\u54c1\u89c4\u683c<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ef7\u683c<\/strong><\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">\u539f\u4f4d\u6742\u4ea4\uff08DAB\u663e\u8272\uff09<\/td><td class=\"has-text-align-center\" data-align=\"center\">HKF5031<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u5f20<\/td><td class=\"has-text-align-center\" data-align=\"center\">240\u5143<\/td><\/tr><\/tbody><\/table><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div 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