{"id":13640,"date":"2024-12-04T10:40:04","date_gmt":"2024-12-04T02:40:04","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=13640"},"modified":"2024-12-17T14:39:33","modified_gmt":"2024-12-17T06:39:33","slug":"mirna%e6%a3%80%e6%b5%8b","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/13640.html","title":{"rendered":"miRNA\u68c0\u6d4b"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"miRNA\u68c0\u6d4b\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">miRNA\u68c0\u6d4b\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/\">\u5b9e\u9a8c\u670d\u52a1<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe5200<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u6837<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKF5013<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\"><\/span><span style=\"color:#666666;\"><\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">\u6d69\u514b<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p><strong>\u670d\u52a1\u4ecb\u7ecd\uff1a<\/strong><br>       \u8367\u5149\u5b9a\u91cfPCR\u662f\u901a\u8fc7\u8367\u5149\u67d3\u6599(SYBR Green)\u6216\u8367\u5149\u6807\u8bb0\u7684\u7279\u5f02\u6027\u7684\u63a2\u9488\uff0c\u5bf9PCR\u4ea7\u7269\u8fdb\u884c\u6807\u8bb0\u8ddf\u8e2a\uff0c\u5b9e\u65f6\u5728\u7ebf\u76d1\u63a7\u53cd\u5e94\u8fc7\u7a0b\uff0c\u7ed3\u5408\u76f8\u5e94\u7684\u8f6f\u4ef6\u53ef\u4ee5\u5bf9\u4ea7\u7269\u8fdb\u884c\u5206\u6790\uff0c\u8ba1\u7b97\u5f85\u6d4b\u6837\u54c1\u6a21\u677f\u7684\u521d\u59cb\u6d53\u5ea6\u3002\u53ef\u4ee5\u7528\u4e8e\u68c0\u6d4bmRNA\u3001lncRNA\u3001circRNA\u3001miRNA\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002\u6700\u7ec8\u6240\u5f97\u7684PCR\u4ea7\u7269\u53ef\u4ee5\u901a\u8fc7\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u5f15\u7269\u7279\u5f02\u6027\u3002<br><strong>\u5b9e\u9a8c\u6d41\u7a0b\uff1a<\/strong><\/p>\n\n\n\n<p>       1.\u63a5\u6536\u6837\u672c<br>       2.\u7ec4\u7ec7\u5300\u6d46\u3001\u7ec6\u80de\u548c\u8840\u6db2\u6837\u672c\u5439\u6253\u88c2\u89e3-RNA\u63d0\u53d6-RNA\u6d53\u5ea6\u6d4b\u5b9a\u2014\u9006\u8f6c\u5f55\u6210cdna\u2014\u8367\u5149\u5b9a\u91cfPCR-(PCR\u4ea7\u7269\u7535\u6cf3)-\u6570\u636e\u5206\u6790 \u5176\u4e2dmRNA\u4e3a\u666e\u901a\u9006\u8f6c\u5f55\uff0clncRNA\u53ef\u9009\u91c7\u53d6\u666e\u901a\u548c\u7279\u5f02\u6027\u9006\u8f6c\u5f55\uff0ccircRNA\u548cmiRNA\u91c7\u53d6\u7279\u5f02\u6027\u9006\u8f6c\u5f55<br>       3.\u4ea4\u4ed8\u7ed3\u679c\uff1a\u5b8c\u6574\u9879\u76ee\u62a5\u544a(\u6269\u589e\u66f2\u7ebf\u3001\u6570\u636e\u5206\u6790\u3001\u5f15\u7269\u4fe1\u606f\u3001\u5b9e\u9a8c\u62a5\u544a)<br><strong>\u5b9e\u9a8c\u5468\u671f\uff1a<\/strong><\/p>\n\n\n\n<p>       60\u4e2a\u5faa\u73af\u5185\uff0c7\u4e2a\u5de5\u4f5c\u65e5\u5185\u51fa\u7ed3\u679c<br><strong>\u6837\u54c1\u8981\u6c42\uff1a<\/strong><br>       1\u3001\u7ec4\u7ec7\uff1a100mg(\u9ec4\u8c46\u5927\u5c0f)\uff0c\u6700\u5c1150mg(\u7eff\u8c46\u5927\u5c0f)\uff0c\u8102\u80aa\u7ec4\u7ec7\uff0c\u7ed3\u7f14\u7ec4\u7ec7\uff0c\u7ea4\u7ef4\u5316\u7ec4\u7ec7\u9002\u5f53\u589e\u52a0\u3002<br>\u3000\u3000<br>       2\u3001\u7ec6\u80de\uff1a1\u00d710^6\u4e2a\u7ec6\u80de,\u8d34\u58c1\u7ec6\u80de\u8212\u5c55\uff0c\u672a\u53d8\u5706;<br>\u3000\u3000<br>       3\u3001\u5168\u8840\uff1a2mL\uff0c\u6700\u5c111mL\u65b0\u9c9c\u8840\u6db2EDTA\u6297\u51dd\u7ba1;<br>\u3000\u3000<br>       4\u3001\u8840\u6e05\uff1a2mL\uff0c\u5e72\u51b0\u8fd0\u8f93;<br>\u3000\u3000<br>       5\u3001\u690d\u7269\uff1a\u53f6\u3001\u679c\u8089\u3001\u79cd\u5b50\u7b49\u9700100mg\uff0c\u6700\u5c1150mg\u3002<br>\u3000\u3000<br>       6\u3001RNA\u6837\u54c1\uff1a\u7eaf\u5ea6OD260\/280\uff0c\u6bd4\u503c\u4e3a1.8-2.0\uff0c\u6d53\u5ea6\u2265500ng\/ul\uff0c\u4f53\u79ef\u226520ul\u3002<br>\u3000\u3000<br>       7\u3001cdna\uff1acdna\u539f\u6db220uL\u3002<br><strong>\u6837\u672c\u8fd0\u8f93\uff1a<\/strong><br>       \u53ef\u9009\u62e9\u4ee5\u4e0b\u4efb\u4f55\u4e00\u79cd\u65b9\u5f0f\u5bc4\u9001\u6807\u672c<br>\u3000\u3000<br>       1.\u52a8\u690d\u7269\u7ec4\u7ec7\u6837\u672c\u3001\u7ec6\u80de\u6837\u672c\u3001\u8840\u6e05\u3001RNA\u3001cDNA\u4ee5\u5e72\u51b0\u8fd0\u8f93;<br>\u3000\u3000<br>       2.\u7ec6\u80de\u6837\u672c\u4e5f\u53ef\u76f4\u63a5\u5bc4\u9001\u57f9\u517b\u74f6(\u5bc6\u5c01\u4fdd\u8bc1\u4e0d\u6c61\u67d3\u3001\u704c\u6ee1\u57f9\u517b\u6db2\u4e0d\u6f0f\u51fa)\uff0c\u5e38\u6e29\u8fd0\u8f93;<br>\u3000\u3000<br>       3.\u5168\u8840\u6837\u672c\u662fEDTA\u6297\u51dd\u7ba14\u5ea6\u8fd0\u8f93\u3002<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u8d27\u53f7<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u540d\u79f0<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u89c4\u683c<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ef7\u683c<\/strong><\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">HKF5013<\/td><td class=\"has-text-align-center\" data-align=\"center\">miRNA\u68c0\u6d4b<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u6837<\/td><td class=\"has-text-align-center\" data-align=\"center\">200<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><br><div><span style=\"margin: 0px; padding: 0px; list-style: none; box-sizing: border-box; -webkit-tap-highlight-color: transparent; letter-spacing: 0px;\"><\/span><\/div><\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":7870,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[216],"tags":[],"class_list":["post-13640","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-technicalservices"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"catce":["sidebar-widgets4"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:6:\"\uffe5200\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u5355\u4f4d\";s:4:\"text\";s:3:\"\u6837\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";b:0;}i:2;a:5:{s:5:\"title\";s:6:\"\u8d27\u53f7\";s:4:\"text\";s:7:\"HKF5013\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:3;a:5:{s:5:\"title\";s:0:\"\";s:4:\"text\";s:0:\"\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:4;a:5:{s:5:\"title\";s:6:\"\u54c1\u724c\";s:4:\"text\";s:6:\"\u6d69\u514b\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}}"],"views":["1221"],"enclosure":["http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\n1266231\nvideo\/mp4\n"],"_dp_original":["8929"],"_edit_lock":["1734417574:2"],"_edit_last":["2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/13640"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=13640"}],"version-history":[{"count":3,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/13640\/revisions"}],"predecessor-version":[{"id":13886,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/13640\/revisions\/13886"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=13640"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=13640"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=13640"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}