{"id":13567,"date":"2024-12-02T17:22:22","date_gmt":"2024-12-02T09:22:22","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=13567"},"modified":"2024-12-02T17:22:24","modified_gmt":"2024-12-02T09:22:24","slug":"cyclin-d1-%e5%85%94%e5%8d%95%e5%85%8b%e9%9a%86%e6%8a%97%e4%bd%93","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/13567.html","title":{"rendered":"Cyclin D1 \u5154\u5355\u514b\u9686\u6297\u4f53"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"Cyclin D1 \u5154\u5355\u514b\u9686\u6297\u4f53\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">Cyclin D1 \u5154\u5355\u514b\u9686\u6297\u4f53<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/products\/kt\/yk\/\">\u4e00\u6297<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/kt\/\">\u6297\u4f53<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/products\/\">\u8bd5\u5242<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\u00a51200<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u89c4\u683c<\/span><span style=\"color:#666666;\">100ul<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKA50044<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u652f<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">HaoKebio<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<figure class=\"wp-block-table\"><table><tbody><tr><th><strong>\u54c1\u724c<\/strong><\/th><th><strong>\u5546\u54c1\u7f16\u7801<\/strong><\/th><th><strong>\u5546\u54c1\u540d\u79f0<\/strong><\/th><th><strong>\u89c4\u683c<\/strong><\/th><th><strong>\u5355\u4f4d<\/strong><\/th><th><strong>\u5355\u4ef7<\/strong><\/th><\/tr><tr><th>HaoKebio<\/th><th>HKA50044<\/th><th>Cyclin D1 \u5154\u5355\u514b\u9686\u6297\u4f53<\/th><th>100ul<\/th><th>\u652f<\/th><th>1200<\/th><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\"><strong>\u80cc\u666f:<\/strong><br>\u7ec6\u80de\u5468\u671f\u86cb\u767d\u4f9d\u8d56\u6027\u6fc0\u9176<br>CDK4\u548cCDK6\u7684\u6d3b\u6027\u53d7\u5230T\u73af\u78f7\u9178\u5316\u3001\u5176\u7ec6\u80de\u5468\u671f\u86cb\u767d\u4f19\u4f34\uff08D\u578b\u7ec6\u80de\u5468\u671f\u86cb\u767d\uff09\u7684\u4e30\u5ea6\u4ee5\u53ca\u4e0eCip\/Kip\u6216INK\u5bb6\u65cf\u86cb\u767d\u7684CDK\u6291\u5236\u5242\u7684\u7ed3\u5408\u7684\u8c03\u63a7\u3002<br>\u4e0d\u6d3b\u8dc3\u7684\u7ec6\u80de\u5468\u671f\u86cb\u767dD\/CDK4\u548cp27 Kip1\u7684\u4e09\u5143\u590d\u5408\u7269\u9700\u8981\u7ec6\u80de\u5916\u7684\u4fc3\u5206\u88c2\u523a\u6fc0\uff0c\u4ee5\u91ca\u653e\u548c\u964d\u89e3p27\uff0c\u540c\u65f6\u4f34\u968f\u7ec6\u80de\u5468\u671f\u86cb\u767dD\u6c34\u5e73\u7684\u4e0a\u5347\uff0c\u4ece\u800c\u5f71\u54cd\u901a\u8fc7\u9650\u5236\u70b9\u7684\u8fdb\u7a0b\u548cRb\u4f9d\u8d56\u7684S\u671f\u8fdb\u5165\u3002<br>\u6d3b\u8dc3\u7684\u7ec6\u80de\u5468\u671f\u86cb\u767dD\/CDK4\u590d\u5408\u7269\u9776\u5411\u89c6\u7f51\u819c\u6bcd\u7ec6\u80de\u7624\u86cb\u767d\u8fdb\u884c\u78f7\u9178\u5316\uff0c\u5141\u8bb8E2F\u8f6c\u5f55\u56e0\u5b50\u7684\u91ca\u653e\uff0c\u4ece\u800c\u6fc0\u6d3bG1\/S\u671f\u57fa\u56e0\u8868\u8fbe\u3002\u5728\u751f\u957f\u56e0\u5b50\u64a4\u56de\u540e\uff0c\u7ec6\u80de\u5468\u671f\u86cb\u767dD\u7684\u86cb\u767d\u6c34\u5e73\u901a\u8fc7\u4e0b\u8c03\u86cb\u767d\u8868\u8fbe\u548c\u78f7\u9178\u5316\u4f9d\u8d56\u6027\u964d\u89e3\u800c\u4e0b\u964d\u3002<p class=\"MsoBodyText\" align=\"justify\" style=\"margin-top:3.3000pt;margin-right:7.7000pt;margin-left:15.5500pt;\ntext-indent:0.7500pt;text-align:justify;text-justify:inter-ideograph;\nline-height:147%;\"><span style=\"font-family: \u5b8b\u4f53; line-height: 147%; font-size: 9pt;\"><\/span><span style=\"font-family: \u5b8b\u4f53; line-height: 147%; font-size: 9pt;\"><\/span><span style=\"font-family: \u5b8b\u4f53; line-height: 147%; letter-spacing: -0.05pt; font-size: 9pt;\"><\/span><\/p><span style=\"font-family: \u5b8b\u4f53; line-height: 147%; font-size: 9pt;\"><\/span><strong>\u4e9a\u7ec6\u80de\u5b9a\u4f4d:<\/strong><br><span style=\"font-family: &quot;Times New Roman&quot;; font-size: 9.5pt;\"><\/span>\u7ec6\u80de\u6838\u3002<br><span style=\"font-family: \u5b8b\u4f53; font-size: 9.5pt;\"><\/span><strong>\u63a8\u8350\u65b9\u6cd5:<\/strong><br><span style=\"font-family: &quot;Times New Roman&quot;; font-size: 9.5pt;\"><\/span> \u9009\u7528Tris-EDTA\u7f13\u51b2\u6db2\uff08\u00a0PH9.0\uff09\u8fdb\u884c\u6297\u539f\u4fee\u590d\uff1b\u00a0\u4e00\u6297\u5728\u5ba4\u6e29\u4e0b\uff084\u2103) \u5b75\u80b2\u8fc7\u591c\u6216\uff0837\u2103\uff09\u6e7f\u76d2\u5b75\u80b22h\u3002<br><span style=\"font-family: \u5b8b\u4f53; font-size: 9.5pt;\"><\/span><strong>\u514d\u75ab\u539f:<\/strong><br><span style=\"font-family: &quot;Times New Roman&quot;; font-size: 9.5pt;\"><\/span>\u4eba\u7ec6\u80de\u5468\u671f\u86cb\u767dD1\u86cb\u767dAla284\u5468\u56f4\u6b8b\u57fa\u7684\u5408\u6210\u80bd\u3002<br><span style=\"font-family: \u5b8b\u4f53; letter-spacing: 0.25pt; font-size: 9.5pt;\"><\/span><strong>\u5b58\u50a8\u6eb6\u6db2:<\/strong><br><span style=\"font-family: &quot;Times New Roman&quot;; font-size: 9.5pt;\"><\/span>10mM HEPES\u94a0(pH 7.5)\u3001150 mM NaCl\u3001100\u00b5g\/ml BSA\u300150%\u7518\u6cb9\u548c\u5c11\u4e8e0.02%\u7684\u53e0\u6c2e\u5316\u94a0\u3002<br><span style=\"font-family: \u5b8b\u4f53; letter-spacing: 0.25pt; font-size: 9.5pt;\"><\/span><strong>\u50a8\u5b58\u6761\u4ef6:<\/strong><br><span style=\"font-family: &quot;Times New Roman&quot;; font-size: 9.5pt;\"><\/span> -20\u00b0C\u00a0\u50a8\u5b58\u4e00\u5e74\u3002<p class=\"MsoBodyText\" style=\"margin-top:9.8500pt;margin-left:15.9500pt;\"><span style=\"font-family: \u5b8b\u4f53; font-size: 9.5pt;\"><\/span><\/p><\/figcaption><\/figure>\n\n\n\n<p><strong>\u5b58\u50a8\u8bf4\u660e<\/strong><strong>:<\/strong><\/p>\n\n\n\n<p>   \u4ee5\u84dd\u51b0\u8fd0\u8f93\u3002\u6536\u8d27\u540e\uff0c\u8fdb\u884c\u5206\u88c5\uff0c\u5e76\u5728-25\u00b0C&nbsp;\u81f3-18\u00b0C&nbsp;\u6761\u4ef6\u4e0b\u50a8&nbsp;\u5b58\u3002\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002<\/p>\n\n\n\n<p><strong>\u63a8\u8350\u7a00\u91ca\u6bd4<\/strong><strong>:<\/strong><\/p>\n\n\n\n<p>   IHC-P:&nbsp;1:100-1:300<\/p>\n\n\n\n<p><strong>\u80cc\u666f\u53c2\u8003\u6587\u732e<\/strong><strong>:<\/strong><\/p>\n\n\n\n<p>\u00a01.Hirai, H. et al. (1995) Mol Cell Biol 15, 2672-81.<\/p>\n\n\n\n<p>\u00a02.\u00a0Sherr, C.J. (1996) Science 274, 1672-7.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p><\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":7870,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[101,99,98],"tags":[],"class_list":["post-13567","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-yk","category-kt","category-products"],"metadata":{"_wp_page_template":["default"],"_wpcom_metas":["a:1:{s:11:\"show_indent\";s:1:\"0\";}"],"views":["1181"],"_canshu":["a:5:{i:0;a:5:{s:5:\"title\";s:6:\"\u4ef7\u683c\";s:4:\"text\";s:6:\"\u00a51200\";s:2:\"f1\";i:16;s:2:\"f2\";s:7:\"#e21f2f\";s:2:\"f3\";b:1;}i:1;a:5:{s:5:\"title\";s:6:\"\u89c4\u683c\";s:4:\"text\";s:5:\"100ul\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:2;a:5:{s:5:\"title\";s:6:\"\u8d27\u53f7\";s:4:\"text\";s:8:\"HKA50044\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}i:3;a:5:{s:5:\"title\";s:6:\"\u5355\u4f4d\";s:4:\"text\";s:3:\"\u652f\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";b:0;}i:4;a:5:{s:5:\"title\";s:6:\"\u54c1\u724c\";s:4:\"text\";s:8:\"HaoKebio\";s:2:\"f1\";s:0:\"\";s:2:\"f2\";s:0:\"\";s:2:\"f3\";s:0:\"\";}}"],"catce":["sidebar-widgets4"],"_dp_original":["13552"],"_edit_lock":["1733131344:2"],"_edit_last":["2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/13567"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=13567"}],"version-history":[{"count":2,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/13567\/revisions"}],"predecessor-version":[{"id":13569,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/13567\/revisions\/13569"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=13567"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=13567"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=13567"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}