{"id":13382,"date":"2024-12-02T11:17:46","date_gmt":"2024-12-02T03:17:46","guid":{"rendered":"https:\/\/www.haokebio.com\/?p=13382"},"modified":"2024-12-02T11:17:47","modified_gmt":"2024-12-02T03:17:47","slug":"%e7%bb%86%e8%83%9e%e5%9f%b9%e5%85%bb","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/13382.html","title":{"rendered":"\u7ec6\u80de\u57f9\u517b"},"content":{"rendered":"\n<section class=\"wp-block-themepark-block-themepark-area block_layout thempark-block undefined\" id=\"\" style=\"padding-bottom:14px;padding-top:28px;\" data-swiper-parallax=\"-700\"><div class=\"block_layout_in\" style=\"\">\n<div class=\"wp-block-column block_layout_in\">\n<div class=\"wp-block-themepark-block-themepark-product-head content_pro bing undefined\" style=\"background-color:#ffffff;margin-bottom:20px;\"><div class=\"tab_pic\" style=\"width:47%\"><div class=\"left_post_pic\"><a class=\" swipebox\" href=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\"><img decoding=\"async\" class=\"lazy lazyload\" data-src=\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\" src=\"https:\/\/www.haokebio.com\/wp-content\/themes\/smartenterprise\/images\/loading.png\" alt=\"\u7ec6\u80de\u57f9\u517b\"\/><\/a><\/div><\/div><div class=\"ds\" style=\"width:48%\">\n<div class=\"wp-block-column\"><header class=\"post_title_head \" style=\"margin-bottom:7px;\"><div class=\"title\"><h1 class=\"h1title\" style=\"font-size:24px;\">\u7ec6\u80de\u57f9\u517b\n\n\n\n\n\n\n\n\n\n\n\n\n<\/h1><\/div><div class=\"description\"><span ><i class=\"fa fa-folder\"><\/i><a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/hkxbsy\/xbsyqt\/\">\u5176\u4ed6<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/\">\u5b9e\u9a8c\u670d\u52a1<\/a> <a  href=\"https:\/\/www.haokebio.com\/category\/technicalservices\/hkxbsy\/\">\u7ec6\u80de\u5b9e\u9a8c<\/a> <\/span><\/div><\/header><ul class=\"csbox_pt \"  ><li class=\"jgss\"style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u4ef7\u683c<\/span><span style=\"font-size:16px;color:#e21f2f;font-weight: bold;\">\uffe52000<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u5355\u4f4d<\/span><span style=\"color:#666666;\">\u6279<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u8d27\u53f7<\/span><span style=\"color:#666666;\">HKF6001<\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\"><\/span><span style=\"color:#666666;\"><\/span><\/li><li style=\"border-bottom:1px solid #e0e0e0;\"><span style=\"font-size:14px;color:#666666;width:125px;\">\u54c1\u724c<\/span><span style=\"color:#666666;\">\u6d69\u514b<\/span><\/li><\/ul>\n\n\n<div class=\"wp-block-buttons\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 has-custom-font-size has-normal-font-size\"><a class=\"wp-block-button__link has-monte-white-color has-luminous-vivid-orange-background-color has-text-color has-background wp-element-button\" style=\"border-radius:10px\" rel=\"http:\/\/wpa.qq.com\/msgrd?v=3&amp;uin=53383090&amp;site=qq&amp;menu=yes\">QQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div><div class=\"layout_badh\"><div class=\"shizhezhao\" style=\"opacity:0.85;background-color:#ffffff;\"><\/div><div class=\"PcOnly themepark-block_layout_ba\"><video id=\"VideoPlayer\" src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" autoplay loop muted defaultmuted=\"\" playsinline><source src=\"http:\/\/data.themepark.com.cn\/2021\/n\/smartenterprise_data\/qy13\/uploads\/2022\/05\/ev_20220524_140257.mp4\" type=\"video\/mp4\"\/><\/video><\/div><\/div><\/section>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-tab thempark-block thempark_tab_box undefined\" style=\"margin-bottom:20px;\" bloop=\"no\" auto=\"no\" mouse=\"click\"><div class=\"content_tab_title\" style=\"background-color:#eaeaea;\"><div class=\"fidxtab\" style=\"background-color:#eaeaea;\"><div class=\"btn active  tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-clipboard-check\" style=\"\"><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-charging-station\" style=\"\"><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><div class=\"btn tabcolor5  \" style=\"\"><span class=\"tabtt\" style=\"\"><i class=\"fas fa fa-fas fa-file-pdf\" style=\"\"><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div><div class=\"swiper-container content_tab_swiper\" style=\"background-color:#ffffff;\"><div class=\"swiper-wrapper\">\n<div class=\"wp-block-column ecdorbox0 swiper-slide tap_s-box active\" style=\"font-size:15px\">\n<p><strong>\u670d\u52a1\u4ecb\u7ecd\uff1a<\/strong><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u8d27\u53f7<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u540d\u79f0<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u89c4\u683c<\/strong><\/td><td class=\"has-text-align-center\" data-align=\"center\"><strong>\u4ef7\u683c<\/strong><\/td><\/tr><tr><td class=\"has-text-align-center\" data-align=\"center\">HKF6001<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u7ec6\u80de\u57f9\u517b<\/td><td class=\"has-text-align-center\" data-align=\"center\">\u6279<\/td><td class=\"has-text-align-center\" data-align=\"center\">2000\u5143<\/td><\/tr><\/tbody><\/table><figcaption class=\"wp-element-caption\">\u3000<strong>\u7b80\u4ecb\uff1a<\/strong><br>\u3000\u3000\u7ec6\u80de\u57f9\u517b\u662f\u91c7\u7528\u65e0\u83cc\u64cd\u4f5c\u7684\u65b9\u6cd5\uff0c\u6a21\u62df\u52a8\u7269\u4f53\u5185\u7684\u751f\u7406\u6761\u4ef6\uff0c\u5728\u4f53\u5916\u8fdb\u884c\u57f9\u517b.\u4f7f\u5176\u4e0d\u65ad\u5730\u751f\u957f\u3001\u7e41\u6b96\uff0c\u4ece\u800c\u89c2\u5bdf\u7ec6\u80de\u7684\u589e\u6b96\u3001\u5206\u5316\u4ee5\u53ca\u7ec6\u80de\u8870\u8001\u7b49\u8fc7\u7a0b\u7684\u751f\u547d\u73b0\u8c61\u3002\u5e38\u89c1\u7684\u7ec6\u80de\u57f9\u517b\u65b9\u5f0f\u53ef\u5206\u4e3a\u8d34\u58c1\u548c\u60ac\u6d6e\u4e24\u79cd\u3002\u7ec6\u80de\u57f9\u517b\u7684\u5e38\u89c4\u64cd\u4f5c\u5305\u62ec\uff1a<br>1)\u7ec6\u80de\u7684\u63a5\u79cd\u548c\u4f20\u4ee3<br>2)\u7ec6\u80de\u8ba1\u6570<br>3)\u7ec6\u80de\u7684\u590d\u82cf\u548c\u51bb\u5b58\u3002<br><strong>\u7ec6\u80de\u57f9\u517b\u65b9\u6cd5\uff1a<\/strong><br>\u3000\u3000<strong>1\u3001\u5b8c\u5168\u57f9\u517b\u57fa\u914d\u7f6e\uff1a<\/strong>\u57f9\u517b\u57fa\u7684\u914d\u5236(\u9700\u5728\u8d85\u51c0\u53f0\u5185\u65e0\u83cc\u64cd\u4f5c)\uff1a10%FBS+90%\u57f9\u517b\u57fa(\u6839\u636e\u7ec6\u80de\u7684\u79cd\u7c7b\u5206\u4e3aDMEM\/F12\u3001DMEM\/HIGH\u3001<br>MEM\/EBSS\u3001\u6539\u826f\u578bRPMI-1640\u57f9\u517b\u57fa)\u3002<br>\u3000\u3000<strong>2\u3001\u7ec6\u80de\u590d\u82cf\uff1a<\/strong><br>       a. \u628a\u51bb\u5b58\u7ba1\u4ece\u6db2\u6c2e\u4e2d\u53d6\u51fa\u6765\uff0c\u7acb\u5373\u6295\u516537\u2103\u6c34\u6d74\u9505\u4e2d\uff0c\u8f7b\u5fae\u6447\u52a8\u3002\u6db2\u4f53\u90fd\u878d\u5316\u540e(\u5927\u69821-1.5\u5206\u949f)\uff0c\u62ff\u51fa\u6765\u55b7\u70b9\u9152\u7cbe\u653e\u5230\u8d85\u51c0\u5de5\u4f5c\u53f0\u91cc\u3002<br>       b.\u628a\u4e0a\u8ff0\u7ec6\u80de\u60ac\u6db2\u5438\u5230\u88c510ml\u57f9\u517b\u57fa15ml\u7684\u79bb\u5fc3\u7ba1\u4e2d(\u7528\u57f9\u517b\u57fa\u628a\u51bb\u5b58\u7ba1\u6d17\u4e00\u904d\uff0c\u628a\u7c98\u5728\u58c1\u4e0a\u7684\u7ec6\u80de\u90fd\u6d17\u4e0b\u6765)\uff0c1000\u8f6c\u79bb\u5fc35\u5206\u949f\u3002<br>       c.\u628a\u4e0a\u6e05\u6db2\u5012\u6389\uff0c\u52a01ml\u57f9\u517b\u57fa\u628a\u7ec6\u80de\u60ac\u6d6e\u8d77\u6765\u3002\u5438\u5230\u88c5\u670910ml\u57f9\u517b\u57fa\u7684\u57f9\u517b\u74f6\u4e2d\u524d\u540e\u5de6\u53f3\u8f7b\u8f7b\u6447\u52a8\uff0c\u4f7f\u57f9\u517b\u74f6\u4e2d\u7684\u7ec6\u80de\u5747\u5300\u5206\u5e03\u3002<br>       d.\u6807\u597d\u7ec6\u80de\u79cd\u7c7b\u548c\u65e5\u671f\u7b49\uff0c\u653e\u5230CO2\u57f9\u517b\u7bb1\u4e2d\u57f9\u517b\uff0c\u7ec6\u80de\u8d34\u58c1\u540e\u6362\u57f9\u517b\u57fa\u3002<br>       e. 3\u5929\u6362\u4e00\u6b21\u57f9\u517b\u57fa\u3002<br>\u3000\u3000<strong>3\u3001\u7ec6\u80de\u4f20\u4ee3\uff1a<\/strong><br>       a. \u57f9\u517b\u74f6\u4e2d\u7684\u7ec6\u80de\u8986\u76d6\u7387\u8fbe\u523080%-90%\u65f6\u8981\u4f20\u4ee3\u3002<br>       b.\u628a\u539f\u6709\u57f9\u517b\u57fa\u5438\u6389\u3002<br>       c.\u52a0\u9002\u5f53\u7684\u80f0\u86cb\u767d\u9176(\u80fd\u8986\u76d6\u7ec6\u80de\u5c31\u884c)\uff0c\u6d88\u53161-2\u5206\u949f\u3002<br>       d.\u7ec6\u80de\u90fd\u53d8\u5706\u540e\u5438\u51fa\u80f0\u9176 \u7ec8\u6b62\u6d88\u5316\u3002<br>       e.\u52a0\u516520ml\u7684\u5b8c\u5168\u57f9\u517b\u57fa\uff0c\u79fb\u6db2\u67aa\u5439\u6253\u7ec6\u80de\uff0c\u628a\u7ec6\u80de\u90fd\u60ac\u6d6e\u8d77\u6765\u3002<br>       f.\u6839\u636e\u7ec6\u80de\u79cd\u7c7b\u628a\u7ec6\u80de\u4f20\u5230\u51e0\u4e2a\u57f9\u517b\u74f6\u4e2d\u3002\u4e00\u822c\uff0c\u764c\u7ec6\u80de\u52064\u4e2a\uff0c\u6b63\u5e38\u7ec6\u80de\u4f203\u4e2a\u3002\u7ee7\u7eed\u57f9\u517b\u3002<br>\u3000\u3000<strong>4\u3001\u7ec6\u80de\u51bb\u5b58\uff1a<\/strong><br>\u628a\u7ec6\u80de\u6d88\u5316\u4e0b\u6765\u5e76\u79bb\u5fc3(\u540c\u4e0a)\u3002\u7528\u914d\u597d\u7684\u51bb\u5b58\u6db2\u628a\u7ec6\u80de\u60ac\u6d6e\u8d77\u6765\uff0c\u5206\u88c5\u5230\u706d\u83cc\u7684\u51bb\u5b58\u7ba1\u4e2d\uff0c\u9759\u6b62\u51e0\u5206\u949f\uff0c\u5199\u660e\u7ec6\u80de\u79cd\u7c7b\uff0c\u51bb\u5b58\u65e5\u671f\u3002<br>4\u210330min\uff0c-20\u210330min\uff0c-80\u2103\u8fc7\u591c\uff0c\u7136\u540e\u653e\u5230\u6db2\u6c2e\u7f50\u4e2d\u4fdd\u5b58\u3002 <br>           <strong>\u51bb\u5b58\u6db2\u7684\u914d\u5236\uff1a<\/strong>70%\u7684\u5b8c\u5168\u57f9\u517b\u57fa+20%FBS+10%DMSO. DMSO\u8981\u6162\u6162\u6ef4\u52a0\uff0c\u8fb9\u6ef4\u8fb9\u6447\u3002<\/figcaption><\/figure>\n\n\n\n<p><strong>\u6837\u672c\u5bc4\u9001\u987b\u77e5\uff1a<\/strong><\/p>\n\n\n\n<p>\u3000\u30001.\u6d3b\u7ec6\u80de\uff1a\u4e00\u5b9a\u8981\u4e0d\u900f\u6c14\u65b9\u74f6\u5bc4\uff0c\u5e38\u6e29;<\/p>\n\n\n\n<p>\u3000\u30002.\u51bb\u5b58\u7ec6\u80de\uff1a\u7eaf\u5e72\u51b0\u4ef6\uff0c\u4fdd\u8bc1\u5e72\u51b0\u5145\u8db3<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox1 swiper-slide tap_s-box\">\n<p>\u65e0<\/p>\n\n\n\n<div class=\"wp-block-themepark-block-themepark-wright content-super-p\" style=\"font-size:16px;line-height:28px;color:#666666;background-color:#f7fcff; border:1px #b2d4e9 solid;padding:10px 21px;\">\n<div class=\"wp-block-column\">\n<p><\/p>\n<\/div>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column ecdorbox2 swiper-slide tap_s-box\">\n<p>\u6682\u65e0<\/p>\n\n\n\n<section class=\"wp-block-themepark-block-themepark-listbox thempark-block themepark_listbox\" style=\"text-align:left;\"><h3 class=\"themepark_listbox_title\" style=\"color:#e21f2f;font-size:18px;padding-left:2%;padding-right:2%;\"><span>\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3><ul class=\"themepark_listbox_list\" style=\"padding-left:2%;padding-right:2%;\"><li style=\"font-size:16px;line-height:26px;margin-bottom:5px;padding-left:31px;\"><a href=\"#\"><i class=\"fas fa-file-pdf\" style=\"color:#e2931d;line-height:26px\"><\/i><\/a><a href=\"#\" style=\"font-size:16px;line-height:26px;margin-bottom:5px;\"><span>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><div class=\"themepark_listbox_ba\" style=\"opacity:1;\"><\/div><\/section>\n\n\n\n<p><\/p>\n<\/div>\n<\/div><\/div><div class=\"tabbottomb\"><\/div><\/div>\n\n\n\n<p style=\"font-size:15px\">\u3000<strong>\u5e38\u7528\u7684\u7ec6\u80de\u57f9\u517b\u57fa\uff1a<\/strong><\/p>\n\n\n\n<figure style=\"font-size:15px\" class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><strong>\u540d\u79f0<\/strong><\/td><td><strong>\u6210\u4efd<\/strong><\/td><td><strong>\u9002\u7528\u7ec6\u80de\u7c7b\u578b<\/strong><\/td><td><strong>\u5907\u6ce8<\/strong><\/td><\/tr><tr><td>MEM<\/td><td>BSS\uff0b12\u79cd\u6c28\u57fa\u9178\uff0b\u8c37\u6c28\u9170\u80fa\uff0b8\u79cd\u7ef4\u751f\u7d20<\/td><td>\u5404\u79cd\u4f20\u4ee3\u7ec6\u80de\u7cfb\u548c\u539f\u4ee3\u7ec6\u80de<\/td><td>\u5728\u6b64\u57fa\u7840\u4e0a\u6539\u826f\u7684\u57f9\u517b\u57fa\u6709BEM\u3001DMEM<\/td><\/tr><tr><td><strong>DMEM<\/strong><\/td><td>\u6539\u826f\u81eaMME\uff0c\u5404\u6210\u5206\u91cf\u52a0\u500d<\/td><td>\u8d34\u9644\u578b\u80bf\u7624\u7ec6\u80de\uff0cCHO\u7ec6\u80de<\/td><td><strong>\u5206\u4f4e\u7cd6\uff085.5mM\uff09\u3001\u9ad8\u7cd6\uff0825mM\uff09\u578b\uff0c\u9ad8\u7cd6\u578b\u8f83\u5e38\u7528<\/strong><\/td><\/tr><tr><td><strong>RPMI-1640<\/strong><\/td><td>BSS\uff0b21\u79cd\u6c28\u57fa\u9178\uff0b\u7ef4\u751f\u7d2011\u79cd\u7b49<\/td><td>\u60ac\u6d6e\u7ec6\u80de\uff0c\u6b63\u5e38\u7ec6\u80de\uff0c\u80bf\u7624\u7ec6\u80de<\/td><td>\u6700\u521d\u9488\u5bf9\u6dcb\u5df4\u7ec6\u80de\u8bbe\u8ba1<\/td><\/tr><tr><td><strong>L15<\/strong><\/td><td>\u534a\u4e73\u7cd6\u66ff\u4ee3\u4e86\u8461\u8404\u7cd6;\u78f7\u9178\u76d0\u7f13\u51b2\u4f53\u7cfb<\/td><td>\u5feb\u901f\u589e\u6b96\u7624\u7ec6\u80de\uff0cCO2\u7f3a\u4e4f<\/td><td>\u4ee5\u534a\u4e73\u7cd6\u4ee3\u66ff\u4e86\u8461\u8404\u7cd6<\/td><\/tr><tr><td><strong>McCoy5A<\/strong><\/td><td>BSS\uff0b40\u79cd\u6210\u5206<\/td><td>\u539f\u4ee3\u7ec6\u80de\uff0c\u6dcb\u5df4\u7ec6\u80de\uff0c\u7ec4\u7ec7\u6d3b\u68c0\u7ec6\u80de<\/td><td>\u6700\u521d\u4e3a\u8089\u7624\u7ec6\u80de\u8bbe\u8ba1<\/td><\/tr><tr><td><strong>DMEM\/F12<\/strong><\/td><td>\u9ad8\u6d53\u5ea6\u4e0e\u6210\u5206\u591a\u6837\u5316<\/td><td>\u539f\u4ee3\u57f9\u517b\u53ca\u66f4\u96be\u517b\u7684\u7ec6\u80de\u7cfb<\/td><td>F12\u4e0eDMEM\u7b49\u4f53\u79ef\u6df7\u5408\uff0c\u53ef\u4f5c\u4e3a\u5f00\u53d1\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u7684\u57fa\u7840\u914d\u65b9<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u3000\u5e38\u7528\u7684\u7ec6\u80de\u57f9\u517b\u57fa\uff1a \u540d\u79f0 \u6210\u4efd \u9002\u7528\u7ec6\u80de\u7c7b\u578b \u5907\u6ce8 MEM BSS\uff0b12\u79cd\u6c28\u57fa\u9178\uff0b\u8c37\u6c28\u9170\u80fa\uff0b8\u79cd\u7ef4\u751f\u7d20 \u5404\u79cd 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