{"id":1282,"date":"2019-12-08T22:19:49","date_gmt":"2019-12-08T14:18:59","guid":{"rendered":"http:\/\/haokebio.com\/1282.html"},"modified":"2019-12-08T22:18:59","modified_gmt":"2019-12-08T14:18:59","slug":"34db8b0028","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1282.html","title":{"rendered":"ELISA\u5b9e\u9a8c\u64cd\u4f5c\u6d41\u7a0b"},"content":{"rendered":"
\n
\n

\u3000\u3000\u6309\u8bd5\u5242\u76d2\u8bf4\u660e\u4e66\u8fdb\u884c\u64cd\u4f5c\uff0c\u4e0d\u540c\u6307\u6807\u64cd\u4f5c\u4e0d\u4e00\u6837\u3002\u5177\u4f53\u4ee5\u8ba2\u8d2d\u7684\u8bd5\u5242\u76d2\u8bf4\u660e\u4e66\u4e3a\u51c6\u3002\u57fa\u672c\u6b65\u9aa4\u5982\u4e0b\uff1a<\/p>\n

\u3000\u30001.\u5305\u88ab\uff1a<\/strong>\u7528\u78b3\u9178\u76d0\u5305\u88ab\u7f13\u51b2\u6db2\u5c06\u6297\u4f53\u7a00\u91ca\u81f3\u86cb\u767d\u8d28\u542b\u91cf\u4e3a1-10μg\/ml\u3002\u5728\u805a\u82ef\u4e59\u70ef\u9176\u6807\u677f\u7684\u6bcf\u5b54\u52a0100μl\uff0c4\u2103\u8fc7\u591c\u3002\u6b21\u65e5\uff0c\u5f03\u53bb\u5b54\u5185\u6eb6\u6db2\uff0c\u7528\u6d17\u6da4\u7f13\u51b2\u6db2\u6d173\u6b21\uff0c\u6bcf\u6b213min\u3002(\u4e00\u822c\u5546\u54c1\u5316\u7684\u8bd5\u5242\u76d2\u4e2d\u5df2\u5305\u88ab\u597d\u6297\u4f53\uff0c\u6b64\u6b65\u9aa4\u53ef\u7701\u7565)<\/p>\n

\u3000\u30002.\u5c01\u95ed\uff1a<\/strong>\u6bcf\u5b54\u52a0\u5165200ul\u5c01\u95ed\u6db2\uff0c37\u2103\u6216\u5ba4\u6e29\u5b75\u80b21-2 h\u3002<\/p>\n

\u3000\u30003.\u6d17\u6da4\uff1a<\/strong>\u5c0f\u5fc3\u63ed\u6389\u5c01\u677f\u819c\uff0c\u653e\u5165\u6d17\u677f\u673a\uff0c\u6d17\u6da43-5\u904d\u3002\u4e5f\u53ef\u4ee5\u624b\u52a8\u6d17\u677f\uff1a\u5f03\u53bb\u6db2\u4f53\uff0c\u6bcf\u5b54\u52a0\u5165300ul\u6d17\u6db2\uff0c\u6d78\u6ce11-2min\uff0c\u5728\u5438\u6c34\u7eb8\u4e0a\u62cd\u5e72\uff0c\u91cd\u590d3-5\u904d\u3002(\u4e00\u822c\u5546\u54c1\u5316\u7684\u8bd5\u5242\u76d2\u4e2d\u5df2\u5305\u88ab\u597d\u6297\u4f53\uff0c\u524d\u4e09\u4e2a\u6b65\u9aa4\u53ef\u7701\u7565)<\/p>\n

\u3000\u30004.\u52a0\u6837\uff1a<\/strong>\u52a0\u9002\u5f53\u7a00\u91ca\u7684\u5f85\u68c0\u6837\u54c1100μl\u4e8e\u4e0a\u8ff0\u5df2\u5305\u88ab\u7684\u53cd\u5e94\u5b54\u4e2d\u3002(\u540c\u65f6\u505a\u7a7a\u767d\u5b54\uff0c\u500d\u6bd4\u7a00\u91ca\u7684\u6807\u51c6\u54c1\u5b54\uff0c\u6709\u6761\u4ef6\u53ef\u52a0\u505a\u9634\u6027\u5bf9\u7167\u5b54\u53ca\u9633\u6027\u5bf9\u7167\u5b54\u4f5c\u4e3a\u8d28\u63a7\u70b9)\u3002<\/p>\n

\u3000\u30005.\u6e29\u80b2\uff1a<\/strong>\u7528\u5c01\u677f\u819c\u5c01\u677f\u540e\u7f6e37\u2103\u5b75\u80b21-2 h\u3002<\/p>\n

\u3000\u30006.\u6d17\u6da4\uff1a<\/strong>\u540c\u6b65\u9aa43\u3002<\/p>\n

\u3000\u30007.\u52a0\u6297\u4f53\uff1a<\/strong>\u4e8e\u5404\u5b54\u4e2d\u52a0\u7a00\u91ca\u597d\u7684\u751f\u7269\u7d20\u5316\u6297\u4f53\u5de5\u4f5c\u6db2100 μl\u3002<\/p>\n

\u3000\u30008.\u6e29\u80b2\uff1a<\/strong>\u7528\u5c01\u677f\u819c\u5c01\u677f\u540e\u7f6e37\u2103\u5b75\u80b21 h\u3002<\/p>\n

\u3000\u30009.\u6d17\u6da4\uff1a<\/strong>\u540c\u6b65\u9aa43\u3002<\/p>\n

\u3000\u300010.\u52a0\u9176\u7ed3\u5408\u7269\uff1a<\/strong>\u4e8e\u5404\u5b54\u4e2d\u52a0\u7a00\u91ca\u597d\u7684\u9176\u7ed3\u5408\u7269\u5de5\u4f5c\u6db2100 μl\u3002<\/p>\n

\u3000\u300011.\u6e29\u80b2\uff1a<\/strong>\u7528\u5c01\u677f\u819c\u5c01\u677f\u540e\u7f6e37\u2103\u907f\u5149\u5b75\u80b230 min\u3002<\/p>\n

\u3000\u300012.\u6d17\u6da4\uff1a<\/strong>\u540c\u6b65\u9aa43\u3002<\/p>\n

\u3000\u300013.\u52a0\u663e\u8272\u5e95\u7269\uff1a<\/strong>\u4e8e\u5404\u5b54\u4e2d\u52a0\u5165TMB\u5e95\u7269\u6eb6\u6db2100 μl\uff0c37\u2103\u907f\u5149\u53cd\u5e9410\uff5e30min\uff0c\u76f4\u5230\u500d\u6bd4\u7a00\u91ca\u7684\u6807\u51c6\u54c1\u5b54\u51fa\u73b0\u660e\u663e\u7684\u989c\u8272\u68af\u5ea6\u4e3a\u6b62\u3002<\/p>\n

\u3000\u300014.\u7ec8\u6b62\u53cd\u5e94\uff1a<\/strong>\u4e8e\u5404\u53cd\u5e94\u5b54\u4e2d\u52a0\u51652M\u786b\u9178100 μl\uff0c\u989c\u8272\u7531\u84dd\u8272\u53d8\u4e3a\u9ec4\u8272\u3002<\/p>\n

\u3000\u300015.\u7ed3\u679c\u6d4b\u5b9a\uff1a<\/strong>10min\u5185\uff0c\u5728\u9176\u6807\u4eea\u4e0a\uff0c\u4e8e450nm\u5904\uff0c\u4ee5\u7a7a\u767d\u5bf9\u7167\u5b54\u8c03\u96f6\u540e\u6d4b\u5404\u5b54OD\u503c\u3002<\/p>\n

\u3000\u3000\u4e8c\u3001ELISA\u7ed3\u679c\u8ba1\u7b97<\/strong><\/p>\n

\u3000\u3000\u6839\u636e\u6807\u51c6\u54c1\u7684\u6d53\u5ea6\u548cOD\u503c\u505a\u6807\u51c6\u66f2\u7ebf\uff0c\u7136\u540e\u6839\u636e\u6807\u51c6\u66f2\u7ebf\u65b9\u7a0b\u8ba1\u7b97\u51fa\u6837\u672c\u6d53\u5ea6\u3002<\/p>\n

\u3000\u3000\u4e09\u3001\u6ce8\u610f\u4e8b\u9879<\/strong><\/p>\n

\u3000\u30001\u3001\u6837\u672c\u8981\u6c42\uff1a<\/strong>\u6837\u672c\u5e94\u6e05\u6f88\u900f\u660e\uff0c\u60ac\u6d6e\u7269\u5e94\u79bb\u5fc3\u53bb\u9664;\u907f\u514d\u4f7f\u7528\u6eb6\u8840\u3001\u9ad8\u8840\u8102\u6837\u672c\u3002\u6837\u672c\u6536\u96c6\u540e\u82e5\u4e0d\u7acb\u5373\u68c0\u6d4b\uff0c\u51bb\u5b58\u4e8e-80°\u51b0\u7bb1\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u3002\u6837\u672c\u6bcf\u6b21\u68c0\u6d4b\u4f7f\u7528\u524d\u8981\u6df7\u5300\uff0c\u7528\u5b8c\u540e\u5c06\u76f8\u5e94\u7248\u5e03\u5c40\u53ca\u6837\u672c\u7f16\u53f7\u8bb0\u5f55\u5728\u5b9e\u9a8c\u672c\u4e0a\uff0c\u6837\u672c\u53ca\u65f6\u653e\u5165\u51b0\u7bb1\u3002<\/p>\n

\u3000\u30002\u3001\u5b9e\u9a8c\u524d\u51c6\u5907<\/strong><\/p>\n

\u3000\u30001.\u5bf9\u5e94\u8ba2\u5355\u627e\u5230\u76f8\u5e94\u7684\u6837\u672c\u548c\u8bd5\u5242\u76d2\u3002<\/p>\n

\u3000\u30002.\u6838\u5bf9\u6837\u672c\u4e2a\u6570\u3001\u79cd\u7c7b\u3001\u7f16\u53f7\u3001\u6837\u672c\u91cf\u662f\u5426\u8db3\u591f\uff0c\u6838\u5bf9\u8bd5\u5242\u76d2\u79cd\u5c5e\u3001\u4e2a\u6570\u3001\u65e5\u671f\uff0c\u786e\u5b9a\u8bd5\u5242\u76d2\u662f\u5426\u591f\u7528\u3001\u6709\u6ca1\u6709\u8fc7\u671f\u3002<\/p>\n

\u3000\u30003.\u6ce8\u610f\u8ba2\u5355\u4e0a\u5907\u6ce8\u680f\u5ba2\u6237\u7684\u8981\u6c42\uff0c\u662f\u5426\u505a\u590d\u5b54\u7b49\u3002(\u4ee5\u4e0a\u4e09\u70b9\u6709\u95ee\u9898\u5728\u5b9e\u9a8c\u524d\u53ca\u65f6\u4e0e\u9500\u552e\u6c9f\u901a)<\/p>\n

\u3000\u3000\u6ce8\uff1a\u5b89\u6392\u597d\u65f6\u95f4\uff0c\u6ce8\u610f\u5b9e\u9a8c\u8282\u594f\uff0c\u505a\u597d\u76f8\u5e94\u7684\u8bb0\u5f55\u5e2e\u52a9\u8bb0\u5fc6\uff0c\u5b89\u6392\u8981\u6709\u6761\u7406\u3002<\/p>\n

\u3000\u30003\u3001\u64cd\u4f5c\u8981\u6c42<\/strong><\/p>\n

\u3000\u30001.\u8bd5\u5242\u76d2\u5728\u4f7f\u7528\u524d\u8981\u5e73\u8861\u5230\u5ba4\u6e29\uff0c\u5e95\u7269\u5728\u4f7f\u7528\u524d\u5e94\u4fdd\u5b58\u5728\u6697\u5904\uff0c\u907f\u5149;<\/p>\n

\u3000\u30002.\u6b63\u5f0f\u5b9e\u9a8c\u524d\u9700\u505a\u9884\u5b9e\u9a8c\u786e\u5b9a\u5408\u9002\u7684\u7a00\u91ca\u500d\u6570\uff0c\u505a\u9884\u5b9e\u9a8c\u524d\u6ce8\u610f\u8bf4\u660e\u4e66\u4e0a\u5efa\u8bae\u7a00\u91ca\u500d\u6570\u3002\u9884\u5b9e\u9a8c\u53cc\u6297\u5939\u5fc3\u6cd5\u9009\u53d6\u6807\u66f2\u6700\u9ad8\u4e24\u4e2a\u70b9\uff0c\u7ade\u4e89\u6cd5\u9009\u53d6\u6807\u66f2\u6700\u9ad8\u70b9\u4e0e\u6700\u4f4e\u70b9\uff0c\u9009\u53d6\u4e24\u4e2a\u6837\u672c\u6839\u636e\u5efa\u8bae\u7a00\u91ca\u500d\u6570\u7a00\u91ca\u4e09\u4e2a\u68af\u5ea6;<\/p>\n

\u3000\u30003.\u5b75\u80b2\u6e29\u5ea6\u4e0e\u65f6\u95f4\u51c6\u786e;\u6d17\u677f\u975e\u5e38\u91cd\u8981\uff0c\u6bcf\u6b65\u6d17\u677f\u90fd\u4e00\u5b9a\u8981\u6d17\u5e72\u51c0;<\/p>\n

\u3000\u30004.\u6807\u51c6\u54c1\u5728\u7528\u524d\u7a00\u91ca\uff0c\u4e14\u7a00\u91ca\u7684\u6bcf\u4e00\u6b65\u90fd\u8981\u6df7\u5300\uff0c\u6ce8\u610f\u6807\u66f2\u7684\u8d77\u59cb\u6d53\u5ea6\u662f\u5426\u4e0e\u91cd\u6eb6\u6d53\u5ea6\u4e00\u81f4;<\/p>\n

\u3000\u30005.\u70b9\u6837\u91cf\u4e00\u5b9a\u8981\u7cbe\u786e\uff0c\u5b8c\u5168\u6253\u5c3d\u79fb\u6db2\u67aa\u5185\u6837\u672c\u4e0e\u8bd5\u5242\uff0c\u5feb\u901f\u3001\u7b49\u91cf\u7684\u5c06\u8bd5\u5242\u5206\u914d\u5230\u5404\u4e2a\u5fae\u5b54\u4e2d;<\/p>\n

\u3000\u30006.\u6297\u4f53\u548cHRP\u7a00\u91ca\u500d\u6570\u8981\u4e25\u683c\u6309\u7167\u8bf4\u660e\u4e66\u89c4\u5b9a\u7a00\u91ca;<\/p>\n

\u3000\u30007.\u5404\u6b65\u9aa4\u4e4b\u95f4\uff0c\u9700\u7528\u5c01\u677f\u8d34\u5bc6\u5c01\u53cd\u5e94\u677f\u907f\u514d\u84b8\u53d1;\u5b9e\u9a8c\u4e00\u65e6\u5f00\u59cb\uff0c\u5e94\u8fde\u7eed\u5b8c\u6210\u6240\u6709\u7684\u5b9e\u9a8c\u6b65\u9aa4\uff0c\u5207\u52ff\u4e2d\u65ad\u3002<\/p>\n

\u3000\u30004\u3001\u5e38\u7528\u5404\u4e2a\u5382\u5bb6\u8bd5\u5242\u76d2\u64cd\u4f5c\u4e0d\u540c\u53ca\u9700\u6ce8\u610f\u7684\u5730\u65b9<\/strong><\/p>\n

\u3000\u3000\u6b23\u535a\u76db\uff1a\u6297\u4f53\u548cHRP\u90fd\u662f30\u500d\u7a00\u91ca\uff0c\u4e14\u5b9e\u9a8c\u4e2d\u6bcf\u6b65\u90fd\u8981\u6d17\u677f5\u6b21<\/p>\n

\u3000\u3000eBiosience(\u5907\u8d27\u9700\u5305\u677f)\uff1a\u5305\u88ab\u6db2\u7528\u524d\u9700\u7a00\u91ca10\u500d\uff0c\u7a00\u91ca\u6db2\u7528\u4e4b\u524d\u8981\u7a00\u91ca5\u500d\uff0c\u5305\u88ab\u6297\u4f53\u68c0\u6d4b\u6297\u4f53\u4ee5\u53caHRP\u90fd\u662f250\u500d\u7a00\u91ca\u3002\u7528\u524d\u8981\u63d0\u524d\u7b97\u597d\u6240\u9700\u8bd5\u5242\u91cf\uff0c\u5305\u88ab4\u5ea6\u8fc7\u591c\uff0c\u53cd\u5e94\u6e29\u5ea6\u4e3a\u5ba4\u6e29\u3002<\/p>\n

\u3000\u3000\u8054\u79d1\/eBiosience(\u5546\u54c1\u5df2\u5305\u88ab)\uff1a\u6807\u51c6\u54c1\u7a00\u91ca\u6db2\u4e0e\u6837\u672c\u7a00\u91ca\u6db2\u4e0d\u662f\u901a\u7528\u7a00\u91ca\u6db2;\u68c0\u6d4b\u7f13\u51b2\u6db2\u7528\u4e4b\u524d\u8981\u7a00\u91ca;\u52a0\u6837\u524d\u9700\u8981\u6d17\u677f\uff0c\u5728\u677f\u5e72\u4e4b\u524d\u52a0\u6837\u4e14\u6807\u51c6\u54c1\u6837\u672c\u548c\u6297\u4f53\u8fde\u7eed\u52a0\u5165\uff0c\u64cd\u4f5c\u4e0d\u8981\u4e2d\u65ad\uff0c\u53cd\u5e94\u6e29\u5ea6\u4e3a\u5ba4\u6e29\u6447\u5e8a\u3002<\/p>\n

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