{"id":1272,"date":"2019-12-08T22:18:44","date_gmt":"2019-12-08T14:17:54","guid":{"rendered":"http:\/\/haokebio.com\/1272.html"},"modified":"2024-09-23T15:20:21","modified_gmt":"2024-09-23T07:20:21","slug":"15cdb12d83","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1272.html","title":{"rendered":"EMSA\u68c0\u6d4b"},"content":{"rendered":"
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\u4e00\u3001\u9879\u76ee\u4ecb\u7ecd<\/strong><\/p>\n

EMSA \u662f\u4e00\u79cd\u7528\u4e8e\u7814\u7a76\u6838\u9178\u548c\u86cb\u767d\u8d28\u4e4b\u95f4\u76f8\u4e92\u4f5c\u7528\u7684\u6280 \u672f\uff0c\u9002\u7528\u4e8e\u8f6c\u5f55\u56e0\u5b50\u4e0e\u542f\u52a8\u5b50\u76f8\u4e92\u4f5c\u7528\u7684\u9a8c\u8bc1\u6027\u5b9e\u9a8c\uff0c\u4e3b\u8981\u662f \u9a8c\u8bc1\u86cb\u767d\u8d28\u4e0e\u7279\u5b9a\u6838\u9178\u5e8f\u5217\u7684\u7ed3\u5408\u7279\u6027\uff0c\u4ece\u800c\u95f4\u63a5\u63a8\u65ad\u5df2\u77e5\u86cb \u767d\u8d28\u7684\u9776\u5e8f\u5217\u6216\u5df2\u77e5\u5e8f\u5217\u7684\u7ed3\u5408\u86cb\u767d\u8d28\u5206\u5b50\u3002<\/p>\n

 <\/p>\n

\u4e8c\u3001\u5b9e\u9a8c\u6b65\u9aa4<\/strong><\/p>\n

1\u3001<\/strong> \u86cb\u767d\u7684\u63d0\u53d6<\/p>\n

\u6d46\u86cb\u767d\u6838\u86cb\u767d\u63d0\u53d6\uff08\u7ec6\u80de\u6838\u86cb\u767d\u4e0e\u7ec6\u80de\u6d46\u86cb\u767d\u62bd\u63d0\u8bd5\u5242\u76d2\uff09<\/p>\n

1.1<\/strong> \u51c6\u5907\u6eb6\u6db2\uff1a\u5ba4\u6e29\u878d\u89e3\u8bd5\u5242\u76d2\u4e2d\u7684\u4e09\u79cd\u8bd5\u5242\uff0c\u6eb6\u89e3\u540e\u7acb\u5373\u653e\u7f6e\u5728\u51b0\u4e0a\uff0c\u6df7\u5300\u3002\u53d6\u9002\u5f53\u91cf\u7684\u7ec6\u80de\u6d46\u86cb\u767d\u62bd\u63d0\u8bd5\u5242A\u5907\u7528\uff0c\u5728\u4f7f\u7528\u524d\u6570\u5206\u949f\u5185\u52a0\u5165PMSF\uff0c\u4f7fPMSF\u7684\u6700\u7ec8\u6d53\u5ea6\u4e3a1mM\u3002\u53d6\u9002\u5f53\u91cf\u7684\u7ec6\u80de\u6838\u86cb\u767d\u62bd\u63d0\u8bd5\u5242\u5907\u7528\uff0c\u5728\u4f7f\u7528\u524d\u6570\u5206\u949f\u5185\u52a0\u5165PMSF\uff0c\u4f7fPMSF\u7684\u6700\u7ec8\u6d53\u5ea6\u4e3a1mM\u3002<\/p>\n

1.2<\/strong> \u5bf9\u4e8e\u8d34\u58c1\u7ec6\u80de\uff1a\u7528PBS\u6d17\u4e00\u904d\uff0c\u7528\u7ec6\u80de\u522e\u5b50\u522e\u4e0b\u7ec6\u80de\uff0c\u6216\u7528EDTA\u6eb6\u6db2\u5904\u7406\u7ec6\u80de\u4f7f\u7ec6\u80de\u4e0d\u518d\u8d34\u58c1\u5f88\u7d27\uff0c\u5e76\u7528\u79fb\u6db2\u5668\u5439\u6253\u4e0b\u7ec6\u80de\u3002\u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\uff0c\u5c3d\u6700\u5927\u52aa\u529b\u5438\u5c3d\u4e0a\u6e05\uff0c\u7559\u4e0b\u7ec6\u80de\u6c89\u6dc0\u5907\u7528\u3002\u5c3d\u91cf\u907f\u514d\u7528\u80f0\u9176\u6d88\u5316\u7ec6\u80de\uff0c\u4ee5\u514d\u80f0\u9176\u964d\u89e3\u9700\u62bd\u63d0\u7684\u76ee\u7684\u86cb\u767d\u3002<\/p>\n

1.3<\/strong> \u5bf9\u4e8e\u60ac\u6d6e\u7ec6\u80de\uff1a\u7528PBS\u6d17\u4e00\u904d\uff0c\u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\uff0c\u5c3d\u6700\u5927\u52aa\u529b\u5438\u5c3d\u4e0a\u6e05\uff0c\u7559\u4e0b\u7ec6\u80de\u6c89\u6dc0\u5907\u7528\u3002<\/p>\n

1.4<\/strong> \u5bf9\u4e8e\u65b0\u9c9c\u7ec4\u7ec7\uff1a<\/p>\n

1 <\/span><\/p>\n

1.1 <\/span><\/p>\n

1.2 <\/span><\/p>\n

1.3 <\/span><\/p>\n

1.4 <\/span><\/p>\n

1.4.1<\/strong> \u628a\u7ec4\u7ec7\u5c3d\u53ef\u80fd\u5207\u6210\u975e\u5e38\u7ec6\u5c0f\u7684\u788e\u7247\u3002\u6309\u716720:1\u7684\u6bd4\u4f8b\u6df7\u5408\u9002\u5f53\u91cf\u7684\u7ec6\u80de\u6d46\u86cb\u767d\u62bd\u63d0\u8bd5\u5242A\u548cB(\u4f8b\u5982200\u5fae\u5347\u7ec6\u80de\u6d46\u86cb\u767d\u62bd\u63d0\u8bd5\u5242A\u4e2d\u52a0\u516510\u5fae\u5347\u62bd\u63d0\u8bd5\u5242B)\u3002\u5e76\u52a0\u5165PMSF\u81f3\u6700\u7ec8\u6d53\u5ea6\u4e3a1mM\u914d\u5236\u6210\u7ec4\u7ec7\u5300\u6d46\u6db2\u3002\u6309\u7167\u6bcf60mg\u7ec4\u7ec7\u52a0\u5165200\u5fae\u5347\u7ec4\u7ec7\u5300\u6d46\u6db2\u7684\u6bd4\u4f8b\u6df7\u5408\u7ec4\u7ec7\u548c\u7ec4\u7ec7\u5300\u6d46\u6db2\uff0c\u5e76\u5728\u73bb\u7483\u5300\u6d46\u5668\u5185\u5145\u5206\u5300\u6d46\u3002\u5300\u6d46\u9700\u5728\u51b0\u6d74\u62164\u2103\u8fdb\u884c\u3002<\/p>\n

1.4.2<\/strong> \u5300\u6d46\u540e\u628a\u5300\u6d46\u6db2\u8f6c\u79fb\u5230\u5851\u6599\u79bb\u5fc3\u7ba1\u5185\uff0c\u51b0\u6d74\u653e\u7f6e15\u5206\u949f\u3002<\/p>\n

1.4.3<\/strong>  4\u2103 1,500g\u79bb\u5fc35\u5206\u949f\u3002\u628a\u4e0a\u6e05\u8f6c\u79fb\u81f3\u4e00\u9884\u51b7\u7684\u5851\u6599\u7ba1\u4e2d\uff0c\u4e3a\u62bd\u63d0\u5f97\u5230\u7684\u90e8\u5206\u7ec6\u80de\u6d46\u86cb\u767d\u3002(\u5438\u4e0a\u6e05\u65f6\u5343\u4e07\u4e0d\u8981\u89e6\u53ca\u6c89\u6dc0\u3002)<\/p>\n

1.4.4<\/strong> \u5bf9\u4e8e\u6c89\u6dc0\uff0c\u5230\u4e86\u8fd9\u4e00\u6b65\u5df2\u7ecf\u5145\u5206\u5300\u6d46\uff0c\u4f46\u5f88\u591a\u7ec6\u80de\u8fd8\u6ca1\u6709\u7834\u788e\u3002<\/p>\n

1.4.5 <\/span><\/p>\n

1.4.6 <\/span><\/p>\n

1.4.7 <\/span><\/p>\n

1.4.8 <\/span><\/p>\n

1.4.9 <\/span><\/p>\n

1.4.10 <\/span><\/p>\n

1.4.11 <\/span><\/p>\n

1.4.12 <\/span><\/p>\n

1.4.13 <\/span><\/p>\n

1.4.14 <\/span><\/p>\n

1.4.15 <\/span><\/p>\n

1.4.16 <\/span><\/p>\n

1.4.17 <\/span><\/p>\n

1.4.18 <\/span><\/p>\n

1.4.19 <\/span><\/p>\n

1.4.20 <\/span><\/p>\n

1.4.21 <\/span><\/p>\n

1.4.22 <\/span><\/p>\n

1.4.23 <\/span><\/p>\n

1.4.24 <\/span><\/p>\n

1.4.25 <\/span><\/p>\n

1.4.26 <\/span><\/p>\n

1.4.27 <\/span><\/p>\n

1.4.28 <\/span><\/p>\n

1.4.29 <\/span><\/p>\n

1.4.30 <\/span><\/p>\n

1.4.31 <\/span><\/p>\n

1.4.32 <\/span><\/p>\n

1.4.33 <\/span><\/p>\n

1.4.34 <\/span><\/p>\n

1.4.35 <\/span><\/p>\n

1.4.36 <\/span><\/p>\n

1.4.37 <\/span><\/p>\n

1.4.38 <\/span><\/p>\n

1.4.39 <\/span><\/p>\n

1.4.40 <\/span><\/p>\n

1.4.41 <\/span><\/p>\n

1.4.42 <\/span><\/p>\n

1.4.5<\/strong> \u6bcf20\u5fae\u5347\u7ec6\u80de\u6c89\u6dc0\u52a0\u5165200\u5fae\u5347\u6dfb\u52a0\u4e86PMSF\u7684\u7ec6\u80de\u6d46\u86cb\u767d\u62bd\u63d0\u8bd5\u5242A\u3002(\u5bf9\u4e8e\u4e8c\u767e\u4e07Hela\u7ec6\u80de\uff0c\u5176\u7ec6\u80de\u6c89\u6dc0\u7684\u4f53\u79ef\u5927\u7ea6\u4e3a20\u5fae\u5347\u621640\u6beb\u514b\u3002)<\/p>\n

1 <\/span><\/p>\n

1.1 <\/span><\/p>\n

1.2 <\/span><\/p>\n

1.3 <\/span><\/p>\n

1.3.1 <\/span><\/p>\n

1.3.2 <\/span><\/p>\n

1.3.3 <\/span><\/p>\n

1.3.4 <\/span><\/p>\n

1 <\/span><\/p>\n

1.3 <\/span><\/p>\n

1.3.4 <\/span><\/p>\n

1.4 <\/span><\/p>\n

1.4.1 <\/span><\/p>\n

1.4.2 <\/span><\/p>\n

1.4.3 <\/span><\/p>\n

1.4.4 <\/span><\/p>\n

1.4.6<\/strong> \u6700\u9ad8\u901f\u5267\u70c8Vortex 5\u79d2\uff0c\u628a\u7ec6\u80de\u6c89\u6dc0\u5b8c\u5168\u60ac\u6d6e\u5e76\u5206\u6563\u5f00\u3002(\u5982\u679c\u7ec6\u80de\u6c89\u6dc0\u6ca1\u6709\u5b8c\u5168\u60ac\u6d6e\u5e76\u5206\u6563\u5f00\uff0c\u53ef\u4ee5\u9002\u5f53\u5ef6\u957fvortex\u65f6\u95f4\u3002)<\/p>\n

1.4.7<\/strong> \u51b0\u6d7410-15\u5206\u949f\u3002<\/p>\n

1.4.8<\/strong> \u52a0\u5165\u7ec6\u80de\u6d46\u86cb\u767d\u62bd\u63d0\u8bd5\u5242B 10\u5fae\u5347\u3002\u6700\u9ad8\u901f\u5267\u70c8Vortex 5\u79d2\uff0c\u51b0\u6d741\u5206\u949f\u3002<\/p>\n

1.4.9<\/strong> \u6700\u9ad8\u901f\u5267\u70c8Vortex 5\u79d2\uff0c4\u2103 12,000-16,000g\u79bb\u5fc35\u5206\u949f\u3002<\/p>\n

1.4.10<\/strong> \u7acb\u5373\u5438\u53d6\u4e0a\u6e05\u81f3\u4e00\u9884\u51b7\u7684\u5851\u6599\u7ba1\u4e2d\uff0c\u5373\u4e3a\u62bd\u63d0\u5f97\u5230\u7684\u7ec6\u80de\u6d46\u86cb\u767d\u3002\u53ef\u4ee5\u7acb\u5373\u4f7f\u7528\uff0c\u4e5f\u53ef\u4ee5\u51bb\u5b58\u3002(\u5343\u4e07\u4e0d\u8981\u89e6\u53ca\u6c89\u6dc0\uff0c\u53ef\u4ee5\u5728\u6c89\u6dc0\u4e0a\u65b9\u4fdd\u7559\u6781\u5c0f\u4f53\u79ef\u7684\u4e0a\u6e05\uff0c\u4ee5\u514d\u89e6\u53ca\u6c89\u6dc0\u3002)<\/p>\n

1.4.11<\/strong>  \u5bf9\u4e8e\u6c89\u6dc0\uff0c\u5b8c\u5168\u5438\u5c3d\u6b8b\u4f59\u7684\u4e0a\u6e05\uff0c\u52a0\u516550\u5fae\u5347\u6dfb\u52a0\u4e86PMSF\u7684\u7ec6\u80de\u6838\u86cb\u767d\u62bd\u63d0\u8bd5\u5242\u3002(\u4e0d\u5438\u5c3d\u4e0a\u6e05\u4f1a\u5e26\u6765\u7ec6\u80de\u6d46\u86cb\u767d\u7684\u6c61\u67d3\u3002)<\/p>\n

1.4.12<\/strong>  \u6700\u9ad8\u901f\u5267\u70c8Vortex 15-30\u79d2\uff0c\u628a\u7ec6\u80de\u6c89\u6dc0\u5b8c\u5168\u60ac\u6d6e\u5e76\u5206\u6563\u5f00\u3002\u7136\u540e\u653e\u56de\u51b0\u6d74\u4e2d\uff0c\u6bcf\u96941-2\u5206\u949f\u518d\u9ad8\u901f\u5267\u70c8Vortex 15-30\u79d2\uff0c\u517130\u5206\u949f\u3002<\/p>\n

1.4.13<\/strong>  4\u2103 12,000-16,000g\u79bb\u5fc310\u5206\u949f\u3002<\/p>\n

1.5<\/strong>  \u6bcf20\u5fae\u5347\u7ec6\u80de\u6c89\u6dc0\u52a0\u5165200\u5fae\u5347\u6dfb\u52a0\u4e86PMSF\u7684\u7ec6\u80de\u6d46\u86cb\u767d\u62bd\u63d0\u8bd5\u5242A\u3002(\u5bf9\u4e8e\u4e8c\u767e\u4e07Hela\u7ec6\u80de\uff0c\u5176\u7ec6\u80de\u6c89\u6dc0\u7684\u4f53\u79ef\u5927\u7ea6\u4e3a20\u5fae\u5347\u621640\u6beb\u514b\u3002)<\/p>\n

1.6<\/strong>  \u6700\u9ad8\u901f\u5267\u70c8Vortex 5\u79d2\uff0c\u628a\u7ec6\u80de\u6c89\u6dc0\u5b8c\u5168\u60ac\u6d6e\u5e76\u5206\u6563\u5f00\u3002(\u5982\u679c\u7ec6\u80de\u6c89\u6dc0\u6ca1\u6709\u5b8c\u5168\u60ac\u6d6e\u5e76\u5206\u6563\u5f00\uff0c\u53ef\u4ee5\u9002\u5f53\u5ef6\u957fvortex\u65f6\u95f4\u3002)<\/p>\n

1.7<\/strong>  \u51b0\u6d7410-15\u5206\u949f\u3002<\/p>\n

1.8<\/strong>  \u52a0\u5165\u7ec6\u80de\u6d46\u86cb\u767d\u62bd\u63d0\u8bd5\u5242B 10\u5fae\u5347\u3002\u6700\u9ad8\u901f\u5267\u70c8Vortex 5\u79d2\uff0c\u51b0\u6d741\u5206\u949f\u3002<\/p>\n

1.9<\/strong>  \u6700\u9ad8\u901f\u5267\u70c8Vortex 5\u79d2\uff0c4\u2103 12,000-16,000g\u79bb\u5fc35\u5206\u949f\u3002<\/p>\n

1.10<\/strong>  \u7acb\u5373\u5438\u53d6\u4e0a\u6e05\u81f3\u4e00\u9884\u51b7\u7684\u5851\u6599\u7ba1\u4e2d\uff0c\u5373\u4e3a\u62bd\u63d0\u5f97\u5230\u7684\u7ec6\u80de\u6d46\u86cb\u767d\u3002\u53ef\u4ee5\u7acb\u5373\u4f7f\u7528\uff0c\u4e5f\u53ef\u4ee5\u51bb\u5b58\u3002(\u5343\u4e07\u4e0d\u8981\u89e6\u53ca\u6c89\u6dc0\uff0c\u53ef\u4ee5\u5728\u6c89\u6dc0\u4e0a\u65b9\u4fdd\u7559\u6781\u5c0f\u4f53\u79ef\u7684\u4e0a\u6e05\uff0c\u4ee5\u514d\u89e6\u53ca\u6c89\u6dc0\u3002)<\/p>\n

1.11<\/strong>  \u5bf9\u4e8e\u6c89\u6dc0\uff0c\u5b8c\u5168\u5438\u5c3d\u6b8b\u4f59\u7684\u4e0a\u6e05\uff0c\u52a0\u516550\u5fae\u5347\u6dfb\u52a0\u4e86PMSF\u7684\u7ec6\u80de\u6838\u86cb\u767d\u62bd\u63d0\u8bd5\u5242\u3002(\u4e0d\u5438\u5c3d\u4e0a\u6e05\u4f1a\u5e26\u6765\u7ec6\u80de\u6d46\u86cb\u767d\u7684\u6c61\u67d3\u3002)<\/p>\n

1.12<\/strong>  \u6700\u9ad8\u901f\u5267\u70c8Vortex 15-30\u79d2\uff0c\u628a\u7ec6\u80de\u6c89\u6dc0\u5b8c\u5168\u60ac\u6d6e\u5e76\u5206\u6563\u5f00\u3002\u7136\u540e\u653e\u56de\u51b0\u6d74\u4e2d\uff0c\u6bcf\u96941-2\u5206\u949f\u518d\u9ad8\u901f\u5267\u70c8Vortex 15-30\u79d2\uff0c\u517130\u5206\u949f\u3002<\/p>\n

1.13<\/strong>  4\u2103 12,000-16,000g\u79bb\u5fc310\u5206\u949f\u3002<\/p>\n

1.14<\/strong>  \u7acb\u5373\u5438\u53d6\u4e0a\u6e05\u81f3\u4e00\u9884\u51b7\u7684\u5851\u6599\u7ba1\u4e2d\uff0c\u5373\u4e3a\u62bd\u63d0\u5f97\u5230\u7684\u7ec6\u80de\u6838\u86cb\u767d\u3002\u53ef\u4ee5\u7acb\u5373\u4f7f\u7528\uff0c\u4e5f\u53ef\u4ee5-70\u2103\u51bb\u5b58\u3002<\/p>\n

2\u3001<\/strong> \u5236\u80f6  \uff081.5mm\u80f6\uff0c\u5927\u7ea630\u5206\u949f\u80f6\u4f1a\u51dd\uff09<\/p>\n

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\u8bd5\u5242<\/span><\/p>\n<\/td>\n

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\u6d53\u5ea65.5\uff05<\/span><\/p>\n<\/td>\n<\/tr>\n

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H<\/span>2<\/span><\/sub>O         ml<\/span><\/p>\n<\/td>\n

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7.50<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

10*TBE       ul<\/span><\/p>\n<\/td>\n

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500<\/span><\/p>\n<\/td>\n<\/tr>\n

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40\uff05\u4e19\u70ef\u9170\u80fa   ml<\/span><\/p>\n<\/td>\n

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1.50<\/span><\/p>\n<\/td>\n<\/tr>\n

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50%\u7518\u6cb9      ul<\/span><\/p>\n<\/td>\n

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500<\/span><\/p>\n<\/td>\n<\/tr>\n

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10\uff05AP       ul<\/span><\/p>\n<\/td>\n

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50<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

TEMED        ul<\/span><\/p>\n<\/td>\n

\n

10<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u603b\u4f53\u79ef       ml<\/span><\/p>\n<\/td>\n

\n

10<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u5f85\u80f6\u5b8c\u5168\u51dd\u56fa\u540e120v\u9884\u7535\u6cf31h\uff0c\u7f13\u51b2\u6db2\u7528\u9884\u51b7\u76840.5×TBE\u3002<\/p>\n

3\u3001<\/strong> \u86cb\u767d\u4e0e\u63a2\u9488\u53cd\u5e94(\u6ce8\uff1a\u86cb\u767d\u6d53\u5ea61 ug\/uL ,\u4e0a\u6837\u4f53\u79ef2 uL)<\/p>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

\u8bd5\u5242\u540d\u79f0<\/span><\/p>\n<\/td>\n

\n

\u9634\u6027\u5bf9\u7167<\/span><\/p>\n<\/td>\n

\n

\u6837\u54c1\u7ec4<\/span><\/p>\n<\/td>\n

\n

\u51b7\u7ade\u4e89<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

10×<\/span>binding buffer<\/span><\/span><\/p>\n<\/td>\n

\n

2<\/span><\/p>\n<\/td>\n

\n

2<\/span><\/p>\n<\/td>\n

\n

2<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

1ug\/ul poly\uff08<\/span>dl.dc<\/span>\uff09<\/span><\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

50\uff05<\/span>glycerol<\/span><\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

1%NP-40<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

100m M Mgcl<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

200m M EDTA<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n

\n

1<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

P<\/span>rotein<\/span><\/p>\n<\/td>\n

\n

–<\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u6807\u8bb0\u63a2\u9488\uff08200 fmol<\/span>\uff09<\/span><\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u672a\u6807\u8bb0\u63a2\u9488(40 pmol)<\/span><\/span><\/p>\n<\/td>\n

\n

0<\/span><\/p>\n<\/td>\n

\n

0<\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

H2O<\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n

\n

+<\/span><\/p>\n<\/td>\n<\/tr>\n

\n

\u603b\u4f53\u79ef<\/span><\/p>\n<\/td>\n

\n

20<\/span><\/p>\n<\/td>\n

\n

20<\/span><\/p>\n<\/td>\n

\n

20<\/span><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u6df7\u5408\u540e\u5ba4\u6e29\u653e\u7f6e20\u5206\u949f\u3002<\/p>\n

4\u3001<\/strong> \u4e0a\u6837<\/p>\n

\u9884\u7535\u6cf3\u5b8c\u540e\u9a6c\u4e0a\u66f4\u6362\u9884\u51b7\u7684\u7535\u6cf3\u7f13\u51b2\u6db2\uff0c\u52a05ul\u76845×\u4e0a\u6837\u7f13\u51b2\u6db2\u5230\u6837\u54c1\u6df7\u5408\u6db2\u4e2d\uff0c\u9a6c\u4e0a\u4e0a\u6837\u7535\u6cf3\u3002180v \uff0c60min\u3002<\/p>\n

5\u3001<\/strong> \u7535\u8f6c\u79fb<\/p>\n

\u5c06\u5e26\u6b63\u7535\u7684\u5c3c\u9f99\u819c\u653e\u51650.5×TBE\u4e2d\u5e73\u886110\u5206\u949f\u3002\u5f85\u7535\u6cf3\u5b8c\u6210\u540e\u5c06\u52a0\u6709\u6837\u54c1\u7684\u6574\u5757\u80f6\u53d6\u4e0b\u7535\u8f6c\u3002\u8f6c\u819c\u7f13\u51b2\u6db2\u4e3a\u9884\u51b7\u76840.5×TBE\u3002300m A 30\u5206\u949f\u3002<\/p>\n

6\u3001<\/strong> \u4ea4\u8054<\/p>\n

\u8f6c\u819c\u5b8c\u6210\u540e\u5c06\u819c\u53d6\u51fa\uff0c\u653e\u5728\u4e00\u5f20\u5e72\u51c0\u7684\u6ee4\u7eb8\u4e0a\uff0c\u505a\u597d\u6807\u8bb0\u3002\u4e8e\u7d2b\u5916\u706f\u4e0b20cm\u5904\u4f5c\u752820\u5206\u949f\u3002<\/p>\n

7\u3001<\/strong> \u5c01\u95ed<\/p>\n

\u5c06\u4ea4\u8054\u5b8c\u6210\u7684\u5c3c\u9f99\u819c\u53d6\u51fa\u653e\u5165\u6d17\u51c0\u7684\u5b75\u80b2\u69fd\uff0c\u7528\u5c01\u95ed\u6db2\u5c01\u95ed20\u5206\u949f\u3002\u671f\u95f4\u653e\u4e8e\u6447\u5e8a\u4e0a\u8f7b\u67d4\u6447\u6643\u3002(\u6162\u6447)\u3002<\/p>\n

8\u3001<\/strong> \u6297\u4f53\u53cd\u5e94<\/p>\n

   \u5012\u6389\u5c01\u95ed\u6db2\u3002\u7528\u5c01\u95ed\u6db2\u5c06\u6297\u4f53\u7a00\u91ca300\u500d\u540e\uff0c\u4e0e\u819c\u5b8c\u5168\u4f5c\u752830\u5206\u949f\u3002\u671f\u95f4\u653e\u4e8e\u6447\u5e8a\u4e0a\u8f7b\u67d4\u6447\u6643\u3002(\u6162\u6447).<\/p>\n

9\u3001<\/strong> \u6d17\u8131<\/p>\n

        \u75281×\u7684\u6d17\u8131\u6db2\u6e05\u6d173\u6b21,\u6bcf\u6b215\u5206\u949f\u3002\u6447\u5e8a\u901f\u5ea6\u52a0\u5927\u3002<\/p>\n

10\u3001<\/strong>\u5e73\u8861<\/p>\n

\u7528\u5e73\u8861\u6db2\u5e73\u88615\u5206\u949f\u3002<\/p>\n

11\u3001<\/strong>ECL\u53d1\u5149\u68c0\u6d4b<\/p>\n

12\u3001<\/strong>\u56fe\u50cf\u5206\u6790\uff08\u7070\u5ea6\u6bd4\u5206\u6790\u7ed3\u679c\u89c1EXCEl\u8868\u683c\uff09<\/p>\n

 <\/p>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u4e00\u3001\u9879\u76ee\u4ecb\u7ecd EMSA \u662f\u4e00\u79cd\u7528\u4e8e\u7814\u7a76\u6838\u9178\u548c\u86cb\u767d\u8d28\u4e4b\u95f4\u76f8\u4e92\u4f5c\u7528\u7684\u6280 \u672f\uff0c\u9002\u7528\u4e8e\u8f6c\u5f55\u56e0\u5b50\u4e0e\u542f\u52a8\u5b50\u76f8\u4e92\u4f5c\u7528\u7684\u9a8c\u8bc1\u6027 […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[216,1,231],"tags":[],"class_list":["post-1272","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-technicalservices","category-uncategorized","category-hkdbswx"],"metadata":{"is_bp":["1"],"views":["1249"],"_edit_last":["2"],"themepark_seo_title":[""],"themepark_seo_description":[""],"themepark_seo_keyword":[""],"_edit_lock":["1727076021:2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1272"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=1272"}],"version-history":[{"count":1,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1272\/revisions"}],"predecessor-version":[{"id":9523,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1272\/revisions\/9523"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=1272"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=1272"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=1272"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}