{"id":1255,"date":"2019-12-08T22:18:17","date_gmt":"2019-12-08T14:17:27","guid":{"rendered":"http:\/\/haokebio.com\/1255.html"},"modified":"2019-12-08T22:17:28","modified_gmt":"2019-12-08T14:17:28","slug":"56d67d4019","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1255.html","title":{"rendered":"\u4e8c\u4ee3\u6d4b\u5e8f\u670d\u52a1\u5185\u5bb9"},"content":{"rendered":"
\n
\n

\u3000\u3000\u4e8c\u4ee3\u6d4b\u5e8f\u670d\u52a1\u9879\u76ee\u5185\u5bb9\u8868\uff1a<\/strong><\/p>\n

\u3000\u3000\u6837\u54c1\u5904\u7406\u65b9\u6cd5\uff1a<\/strong><\/p>\n

\n\n\n\n\n\n\n\n
\u6807\u672c\u7c7b\u578b<\/strong><\/td>\n\u53d6\u6837\u8981\u6c42<\/strong><\/td>\n\u4fdd\u5b58\u6e29\u5ea6\u53ca\u9001\u6837\u8981\u6c42<\/strong><\/td>\n<\/tr>\n
\u8840\u6db2<\/td>\n\u53d6\u8db3\u91cf\u8840\u6db2\u540e\u7f6e\u4e8e\u542b\u6709\u6297\u51dd\u5242EDTA\uff0c\u5e76\u4e14\u7528\u6db2\u6c2e\u9884\u51bb\u8fc7\u7684\u88c5\u8840\u7ba1\u4e2d\uff0c-80°C\u4fdd\u5b58<\/td>\n-80°C\uff0c\u5e72\u51b0\u9001\u6837<\/td>\n<\/tr>\n
\u7ec6\u80de<\/td>\n\u7ec6\u80de\u60ac\u6d6e\uff0c\u7528Pbs\u6e05\u6d17\u4e24\u6b21\uff0c\u79bb\u5fc3\u540e\u53bb\u4e0a\u6e05\uff0c\u75281mL\u7684Trizol\u91cd\u60ac\u6d6e\uff0c-80°C\u4fdd\u5b58<\/td>\n-80°C\uff0c\u5e72\u51b0\u9001\u6837<\/td>\n<\/tr>\n
\u666e\u901a\u6837\u672c\uff08\u7ec4\u7ec7\u3001\u7caa\u4fbf\u3001\u571f\u58e4\uff09<\/td>\n\u65b0\u9c9c\u6837\u672c\u53d6\u6837\u540e\u76f4\u63a5\u7f6e\u4e8e\u7528\u6db2\u6c2e\u9884\u51bb\u8fc7\u7684\u79bb\u5fc3\u7ba1\u4e2d\uff0c\u91cd\u653e\u56de\u6db2\u6c2e\u4e2d\u901f\u51bb\uff0c\u7136\u540e-80°C\u4fdd\u5b58<\/td>\n-80°C\uff0c\u5e72\u51b0\u9001\u6837<\/td>\n<\/tr>\n
\u75c5\u6bd2DNA\uff0c\u5927\u91cf\u6c34\u6837<\/td>\n\u9700\u63d0\u53d6DNA\uff0c-80°C\u4fdd\u5b58<\/td>\n-80°C\uff0c\u5e72\u51b0\u9001\u6837<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

\u3000\u3000\u771f\u6838\u8f6c\u5f55\u7ec4(\u6709\u53c2\/\u65e0\u53c2)\u5206\u6790\u5e38\u89c1\u95ee\u9898\uff1a<\/strong><\/p>\n

\u3000\u30001\u3001\u80fd\u5426\u901a\u8fc7\u8f6c\u5f55\u7ec4\u7814\u7a76\u627e\u5230\u6211\u60f3\u8981\u7684\u57fa\u56e0\uff0c\u4ee5\u53ca\u5b83\u5728\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u8868\u8fbe\u5dee\u5f02?<\/p>\n

\u3000\u3000\u53ef\u4ee5\uff0c\u4f46\u524d\u63d0\u662f\u6837\u672c\u7269\u79cd\u4e2d\u7684\u786e\u5b58\u5728\u8be5\u57fa\u56e0\u3002\u5bf9\u8f6c\u5f55\u7ec4\u6570\u636e\u8fdb\u884c\u6837\u672c\u95f4\u5dee\u5f02\u5206\u6790\u53ef\u4ee5\u5f97\u5230\u57fa\u56e0\u7684\u8868\u8fbe\u91cf\u5dee\u5f02\uff0c\u4ee5\u53ca\u5dee\u5f02\u662f\u5426\u663e\u8457\u3002\u6709\u53c2\u8f6c\u5f55\u80fd\u591f\u76f4\u63a5\u901a\u8fc7\u7ed3\u679c\u6587\u4ef6\u627e\u5230\u76ee\u7684\u57fa\u56e0\u7684\u540d\u79f0\u53ca\u76f8\u5173\u4fe1\u606f\uff0c\u65e0\u53c2\u8f6c\u5f55\u5219\u9700\u8981\u901a\u8fc7BlastX\u8fdb\u884c\u6bd4\u5bf9\u5bfb\u627e\u3002<\/p>\n

\u3000\u30002\u3001\u80fd\u5426\u901a\u8fc7\u8f6c\u5f55\u7ec4\u7814\u7a76\u627e\u5230\u9488\u5bf9\u67d0\u4e00\u7279\u5b9a\u529f\u80fd\u7684\u57fa\u56e0\uff0c\u4ee5\u53ca\u5206\u6790\u5b83\u7684\u8868\u8fbe\u60c5\u51b5?<\/p>\n

\u3000\u3000\u53ef\u4ee5\u3002\u8f6c\u5f55\u7ec4\u5206\u6790\u901a\u8fc7GO\u6570\u636e\u5e93\u6bd4\u5bf9\uff0c\u80fd\u591f\u5f97\u5230\u4e0eGO\u6570\u636e\u5e93\u4e2d\u4e0d\u540c\u529f\u80fd\u76f8\u5173\u7684\u57fa\u56e0\u4fe1\u606f\uff0c\u4ee5\u53ca\u5176\u5728\u5b9e\u9a8c\u7ec4\u4e2d\u7684\u4e0a\u3001\u4e0b\u8c03\u60c5\u51b5<\/p>\n

\u3000\u30003\u3001\u53ef\u53d8\u526a\u5207\u90fd\u6709\u4e9b\u4ec0\u4e48\u526a\u5207\uff0c\u901a\u8fc7\u8f6c\u5f55\u7ec4\u5206\u6790\u6211\u80fd\u5f97\u5230\u4ec0\u4e48?<\/p>\n

\u3000\u3000\u53ef\u53d8\u526a\u5207\u5171\u5206\u4e3a5\u79cd\uff1aSE(\u5916\u663e\u5b50\u8df3\u8dc3)\u3001RI(\u5185\u542b\u5b50\u6ede\u7559)\u3001MXE(\u5916\u663e\u5b50\u4e92\u65a5)\u3001A5SS(5‘\u7aef\u5916\u663e\u5b50\u53ef\u53d8\u526a\u5207)\u4ee5\u53caA3SS(3’\u7aef\u5916\u663e\u5b50\u53ef\u53d8\u526a\u5207)\u3002\u53ef\u53d8\u526a\u5207\u662f\u8c03\u8282\u57fa\u56e0\u8868\u8fbe\u548c\u4ea7\u751f\u86cb\u767d\u8d28\u7ec4\u591a\u6837\u6027\u7684\u91cd\u8981\u673a\u5236\uff0c\u540c\u65f6\u4e5f\u662f\u8f6c\u5f55\u7ec4\u7814\u7a76\u7684\u4e00\u4e2a\u91cd\u8981\u5185\u5bb9\u3002\u8f6c\u5f55\u7ec4\u5206\u6790\u80fd\u591f\u5206\u6790\u51fa\u67d0\u4e00\u7279\u5b9a\u53ef\u53d8\u526a\u5207\u6240\u5904\u7684\u57fa\u56e0\u540d\u79f0\uff0c\u67d3\u8272\u4f53\u4f4d\u7f6e\uff0c\u5728\u4e0d\u540c\u6837\u672c\u4e4b\u95f4\u7684\u8868\u8fbe\u91cf\u5dee\u5f02\u4ee5\u53ca\u5dee\u5f02\u663e\u8457\u6027\u7b49<\/p>\n

\u3000\u30004\u3001SNP\u3001Indel\u4e3b\u8981\u6307\u4ec0\u4e48?<\/p>\n

\u3000\u3000SNP\u548cIndel\u5206\u522b\u6307\u5355\u6838\u82f7\u9178\u591a\u6001\u6027(\u5355\u78b1\u57fa\u53d8\u5f02)\u548c\u6838\u82f7\u9178\u63d2\u5165\u7f3a\u5931(\u591a\u78b1\u57fa\u63d2\u5165\/\u7f3a\u5931)\uff0c\u8868\u660e\u4e86\u5b9e\u9a8c\u7ec4\u5728\u7ecf\u8fc7\u5904\u7406\u540e\u7684\u57fa\u56e0\u53d8\u5f02\u60c5\u51b5\u3002\u901a\u8fc7\u8f6c\u5f55\u7ec4\u7814\u7a76\u80fd\u591f\u5f97\u5230\u4e0d\u540c\u57fa\u56e0\u4e0d\u540c\u4f4d\u70b9\u4e0a\u53d1\u751f\u7684\u53d8\u5f02\u548c\u5f71\u54cd\u60c5\u51b5\uff0c\u5bf9\u80bf\u7624\u7b49\u7814\u7a76\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<\/p>\n

\u3000\u3000\u7ed3\u679c\u5c55\u793a\uff1a<\/strong><\/p>\n

\"\u4e8c\u4ee3\u6d4b\u5e8f\u670d\u52a1\u5185\u5bb9\"<\/strong><\/p>\n

\u3000\u3000\u56fe\u4e00\uff1a\u5dee\u5f02\u57fa\u56e0\u7b5b\u9009\u3002\u706b\u5c71\u56fe\u80fd\u591f\u76f4\u89c2\u7684\u5c55\u793a\u5177\u6709\u663e\u8457\u6027\u5dee\u5f02\u8868\u8fbe\u7684\u57fa\u56e0\u6570\u76ee<\/p>\n

\"\u4e8c\u4ee3\u6d4b\u5e8f\u670d\u52a1\u5185\u5bb9\"<\/p>\n

\u3000\u3000\u56fe\u4e8c\uff1a\u6570\u636e\u8d28\u63a7\u3002\u6d4b\u5e8f\u5f97\u5230\u7684\u539f\u59cb\u6d4b\u5e8f\u5e8f\u5217(Sequenced Reads)\u6216\u8005raw reads\uff0c\u91cc\u9762\u542b\u6709\u5e26\u63a5\u5934\u7684\u3001\u4f4e\u8d28\u91cf\u7684reads\uff0c\u4e3a\u4e86\u4fdd\u8bc1\u4fe1\u606f\u5206\u6790\u8d28\u91cf\uff0c\u5fc5\u987b\u5bf9raw reads\u8fc7\u6ee4\uff0c\u5f97\u5230clean reads\uff0c\u540e\u7eed\u5206\u6790\u90fd\u57fa\u4e8eclean reads\u3002\u5728\u6d4b\u5e8f\u8fc7\u7a0b\u4e2d\u4f1a\u5b58\u5728\u4e00\u5b9a\u7684\u9519\u8bef\u7387\uff0c\u6d4b\u5e8f\u9519\u8bef\u7387\u5206\u5e03\u68c0\u67e5\u53ef\u4ee5\u53cd\u6620\u6d4b\u5e8f\u6570\u636e\u7684\u8d28\u91cf\u3002<\/p>\n

\u3000\u300016S\/18S\/ITS\u6269\u589e\u5b50\u6d4b\u5e8f\u5e38\u89c1\u95ee\u9898\uff1a<\/strong><\/p>\n

\u3000\u30001\u3001\u6269\u589e\u5b50\u4e0e\u5b8f\u57fa\u56e0\u7ec4\u7684\u5dee\u522b?<\/p>\n

\u3000\u3000\u6269\u589e\u5b50\u4e3b\u8981\u5206\u4e3a16S\u300118S\u3001ITS\u6d4b\u5e8f\uff0c\u5173\u6ce8\u73af\u5883\u6837\u672c\u4e2d\u7684\u7269\u79cd\u7ec4\u6210\uff0c\u8be5\u6280\u672f\u7269\u7f8e\u4ef7\u5ec9\uff0c\u5b9e\u7528\u6027\u9ad8\u3002\u5b8f\u57fa\u56e0\u7ec4\u5173\u6ce8\u73af\u5883\u6837\u672c\u4e2d\u7684\u7269\u79cd\u7ec4\u6210\u3001\u529f\u80fd\u7ec4\u6210\u53ca\u4ee3\u8c22\u901a\u8def\u7b49\u4fe1\u606f\uff0c\u8be5\u6280\u672f\u6df1\u5165\u6316\u6398\u73af\u5883\u6837\u672c\u529f\u80fd\u5c42\u9762\u7684\u4fe1\u606f\u3002<\/p>\n

\u3000\u30002\u3001\u6837\u672c\u9001\u5230\u516c\u53f8\uff0c\u4ec0\u4e48\u65f6\u5019\u53ef\u4ee5\u62ff\u5230\u7ed3\u679c?<\/p>\n

\u3000\u3000\u81ea\u6837\u672c\u68c0\u6d4b\u5408\u683c\u4e4b\u540e\u5f00\u59cb\u8ba1\u7b97\u5468\u671f\uff0c\u5305\u62ec\u5efa\u5e93\u3001\u6d4b\u5e8f\u3001\u6570\u636e\u5206\u6790\u3001\u7ed3\u9898\u3002\u6269\u589e\u5b50\u5468\u671f\u4e00\u4e2a\u6708\u5de6\u53f3\u3002<\/p>\n

\u3000\u30003\u3001\u6269\u589e\u5b50\u6d4b\u5e8f\u4e3b\u8981\u80fd\u591f\u5f97\u5230\u7684\u7ed3\u679c\u6709\u54ea\u4e9b?<\/p>\n

\u3000\u3000\u6269\u589e\u5b50\u6d4b\u5e8f\u4e3b\u8981\u662f\u80fd\u591f\u4e0d\u901a\u8fc7\u5206\u79bb\u7b5b\u9009\uff0c\u76f4\u63a5\u63d0\u53d6\u73af\u5883\u4e2d\u7684\u603bDNA\u8fdb\u884c\u6d4b\u5e8f\uff0c\u5f97\u5230\u67d0\u4e00\u751f\u6001\u73af\u5883\u6216\u5904\u7406\u4e2d\u5fae\u751f\u7269\u7fa4\u843d\u7684\u7269\u79cd\u5206\u5e03\u3001α\u591a\u6837\u6027(\u6837\u672c\u5185)\u4ee5\u53caβ\u591a\u6837\u6027(\u6837\u672c\u95f4);\u540c\u65f6\uff0c\u5f53\u6837\u672c\u6765\u81ea\u4e8e\u73af\u5883\u5982\u571f\u58e4\u3001\u6c34\u6837\u7b49\uff0c\u8fd8\u53ef\u4ee5\u5206\u6790\u73af\u5883\u56e0\u5b50\u4e0e\u8be5\u6837\u672c\u5185\u7269\u79cd\u4e4b\u95f4\u7684\u5173\u8054\u6027\u3002<\/p>\n

\u3000\u3000\u7ed3\u679c\u5c55\u793a\uff1a<\/strong><\/p>\n

\"\u4e8c\u4ee3\u6d4b\u5e8f\u670d\u52a1\u5185\u5bb9\"<\/strong><\/p>\n

\u3000\u3000\u56fe\u4e09\u3002\u7269\u79cd\u8fdb\u5316\u6811\u3002\u80fd\u591f\u5f97\u5230\u5c5e\u6c34\u5e73\u524d100\u5c5e\u7684\u4ee3\u8868\u5e8f\u5217\u95f4\u7684\u8fdc\u8fd1\u5173\u7cfb\u53ca\u5728\u4e0d\u540c\u6837\u672c\u95f4\u7684\u4e30\u5ea6\u4fe1\u606f\u3002<\/p>\n

\"\u4e8c\u4ee3\u6d4b\u5e8f\u670d\u52a1\u5185\u5bb9\"<\/p>\n

\u3000\u3000\u56fe\u56db\uff1aPCA\u4e3b\u6210\u5206\u5206\u6790\u3002\u80fd\u591f\u6700\u5927\u7a0b\u5ea6\u53cd\u6620\u6837\u672c\u95f4\u5dee\u5f02\u3002\u5982\u679c\u6837\u672c\u7684\u7fa4\u843d\u7ec4\u6210\u8d8a\u76f8\u4f3c\uff0c\u5219\u5b83\u4eec\u5728PCA\u56fe\u4e2d\u7684\u8ddd\u79bb\u8d8a\u63a5\u8fd1\u3002<\/p>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u3000\u3000\u4e8c\u4ee3\u6d4b\u5e8f\u670d\u52a1\u9879\u76ee\u5185\u5bb9\u8868\uff1a \u3000\u3000\u6837\u54c1\u5904\u7406\u65b9\u6cd5\uff1a \u6807\u672c\u7c7b\u578b \u53d6\u6837\u8981\u6c42 \u4fdd\u5b58\u6e29\u5ea6\u53ca\u9001\u6837\u8981\u6c42 \u8840\u6db2 \u53d6\u8db3\u91cf\u8840\u6db2\u540e\u7f6e […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1255","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["1106"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1255"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=1255"}],"version-history":[{"count":0,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1255\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=1255"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=1255"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=1255"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}