{"id":1253,"date":"2019-12-08T22:18:05","date_gmt":"2019-12-08T14:17:15","guid":{"rendered":"http:\/\/haokebio.com\/1253.html"},"modified":"2019-12-08T22:17:15","modified_gmt":"2019-12-08T14:17:15","slug":"053e4304f6","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1253.html","title":{"rendered":"\u4e00\u4ee3\u6d4b\u5e8f\u79d1\u6280\u670d\u52a1\u5185\u5bb9"},"content":{"rendered":"
\n
\n
\n\n\n\n\n\n\n\n\n
\u670d\u52a1\u5185\u5bb9<\/td>\n\u670d\u52a1\u8bf4\u660e<\/td>\n\u5468\u671f(\u5929)<\/td>\n\u4ef7\u683c<\/td>\n<\/tr>\n
\u83cc\u6db2\u6d4b\u5e8f\/\u8d28\u7c92\u6d4b\u5e8f\/PCR\u4ea7\u7269\u6d4b\u5e8f<\/td>\n<10\u53cd\u5e94<\/td>\n\u5f85\u5b9a<\/td>\n25\u5143\/\u53cd\u5e94<\/td>\n<\/tr>\n
≥10\u53cd\u5e94<\/td>\n<\/tr>\n
≥100\u53cd\u5e94<\/td>\n<\/tr>\n
\u5927\u89c4\u6a21\u6d4b\u5e8f<\/td>\n<\/tr>\n
\u8bbe\u8ba1\u5408\u6210\u6d4b\u5e8f\u5f15\u7269<\/td>\n\u5408\u6210\u91cf2OD\uff0cPAGE\u7eaf\u5316<\/td>\n1.2\u5143\/\u78b1\u57fa<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n

 <\/div>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u6837\u54c1\u7c7b\u578b<\/td>\n\u5177\u4f53\u8981\u6c42<\/td>\n\u5907\u6ce8<\/td>\n<\/tr>\n
\u83cc\u6db2<\/td>\n1. \u6ce8\u660e\u6297\u6027\u7c7b\u578b\uff0c\u6211\u4eec\u63d0\u4f9bAmp,Kan,Chl,Tet\uff0c\u5176\u4f59\u6297\u751f\u7d20\u8bf7\u81ea\u5e26\u5e76\u6ce8\u660e\u4f7f\u7528\u65b9\u6cd5\u3002 <\/td>\n\u6240\u6709\u6837\u54c1\u7684\u7f16\u53f7\u5c3d\u91cf\u7b80\u6d01\u660e\u4e86\uff0c\u907f\u514d\u5bb9\u6613\u6df7\u6dc6\u7684\u7f16\u53f7\u548c\u7279\u6b8a\u7b26\u53f7\uff0c\u6240\u6709\u6837\u54c1\u5747\u5e94\u63d0\u4f9b\u76f8\u5e94\u7684\u5f15\u7269\u4fe1\u606f\uff0c\u5bc4\u6837\u54c1\u65f6\uff0c\u8bf7\u6ce8\u610f\u505a\u597d\u7f13\u51b2\u4fdd\u62a4\u5305\u88c5\uff0c\u9632\u6b62\u6837\u54c1\u53d7\u5230\u6324\u538b\u800c\u788e\u88c2\u3002<\/td>\n<\/tr>\n
2. \u542b\u4f4e\u62f7\u5907\u8d28\u7c92\u7684\u83cc\u6db2\uff0c\u8bf7\u63d0\u4f9b\u7ea61ug\u7eaf\u5316\u8d28\u7c92\u3002 <\/td>\n<\/tr>\n
3. \u9700\u57f9\u517b\u7684\u83cc\u6db2\uff1a\u63d0\u4f9b\u7684\u91cf\u4e0d\u5c11\u4e8e200μl\u3002<\/td>\n<\/tr>\n
4. \u957f\u9014\u8fd0\u8f93\u5c3d\u91cf\u63d0\u4f9b\u7a7f\u523a\u83cc\u3002<\/td>\n<\/tr>\n
5\u3001\u65b0\u9c9c\u83cc\u6db2\u6613\u4e8e\u57f9\u517b\uff0c\u53ef\u4ee5\u83b7\u5f97\u66f4\u591a\u7684DNA\uff0c\u540c\u65f6\u66f4\u5927\u9650\u5ea6\u4fdd\u8bc1\u83cc\u79cd\u7684\u7eaf\u5ea6\u3002<\/td>\n<\/tr>\n
\u8d28\u7c92<\/td>\n1. \u6eb6\u4e8e\u9002\u91cf\u4f53\u79ef\u53cc\u84b8\u6c34\u4e2d\uff0c\u5efa\u8bae\u7528\u76f8\u5173\u8d28\u7c92\u63d0\u53d6\u8bd5\u5242\u76d2\u3002 <\/td>\n<\/tr>\n
2. \u7535\u6cf3\u68c0\u6d4b\u6d53\u5ea6\u5927\u4e8e50ng\/ul\uff0c\u4f53\u79ef\u5927\u4e8e20ul\u3002 <\/td>\n<\/tr>\n
3. \u5efa\u8bae\u540c\u65f6\u63d0\u4f9b0.5~1ml\u542b\u6709\u76f8\u5e94\u8d28\u7c92\u7684\u83cc\u6db2\u5907\u7528\u3002 <\/td>\n<\/tr>\n
4. \u7279\u6b8a\u8f7d\u4f53\u8bf7\u81ea\u5907\u5f15\u7269\u6216\u63d0\u4f9b\u76f8\u5e94\u4fe1\u606f\u5408\u6210\u5f15\u7269\u3002<\/td>\n<\/tr>\n
\u672a\u7eaf\u5316PCR\u4ea7\u7269<\/td>\n1. \u7247\u6bb5\u957f\u5ea6\u5927\u4e8e100bp\uff0c\u5c0f\u4e8e2k bp\uff1b <\/td>\n<\/tr>\n
2. \u8bf7\u63d0\u4f9b30ul\u4ee5\u4e0a\u7684PCR\u6269\u589e\u4ea7\u7269\uff08\u603b\u91cf1-2ug\uff09\uff1b <\/td>\n<\/tr>\n
3. \u53d63ul\u7535\u6cf3\u68c0\u6d4b\u4e3a\u660e\u4eae\u7684\u4e00\u6761\u5e26\uff0c\u65e0\u6742\u5e26\u3002<\/td>\n<\/tr>\n
\u5df2\u7eaf\u5316PCR\u4ea7\u7269<\/td>\n1. PCR\u4ea7\u7269\u6eb6\u4e8e\u53cc\u84b8\u6c34\u4e2d\uff0c\u7535\u6cf3\u68c0\u6d4b\u6761\u5e26\u5355\u4e00\uff1b <\/td>\n<\/tr>\n
2. \u6d53\u5ea6\u5927\u4e8e30ng\/ul\uff0c\u4f53\u79ef\u5927\u4e8e20ul\uff0c\u957f\u7247\u6bb5\u9002\u5f53\u589e\u5927\u9001\u6837\u91cf\uff1b <\/td>\n<\/tr>\n
3. \u6ce8\u660ePCR\u4ea7\u7269\u7247\u6bb5\u957f\u5ea6\u3002<\/td>\n<\/tr>\n
\u81ea\u5e26\u5f15\u7269<\/td>\n1. \u8bf7\u6ce8\u660e\u6d53\u5ea6\uff0c\u6700\u597d\u8c03\u6574\u52305pmol\/ul (\u53735uM)\uff0c\u4f53\u79ef\u5e94\u5927\u4e8e20ul\uff1b <\/td>\n<\/tr>\n
2. \u4e0a\u4e0b\u6e38\u5f15\u7269\u9700\u5206\u5f00\u5b58\u653e\uff0c\u5e76\u6807\u6ce8\u6e05\u695a\uff1b <\/td>\n<\/tr>\n
3. \u968f\u673a\u5f15\u7269\u3001\u7b80\u5e76\u5f15\u7269\u3001\u8367\u5149\u6807\u8bb0\u5f15\u7269\u4e0d\u80fd\u7528\u4e8e\u6d4b\u5e8f\u3002<\/td>\n<\/tr>\n
\u5907\u6ce8\uff1a\u8be6\u7ec6\u5185\u5bb9\u89c1\u4ea7\u54c1\u76ee\u5f55<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u670d\u52a1\u5185\u5bb9 \u670d\u52a1\u8bf4\u660e \u5468\u671f(\u5929) \u4ef7\u683c \u83cc\u6db2\u6d4b\u5e8f\/\u8d28\u7c92\u6d4b\u5e8f\/PCR\u4ea7\u7269\u6d4b\u5e8f <10\u53cd\u5e94 \u5f85\u5b9a 25\u5143\/\u53cd\u5e94 […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1253","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["692"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1253"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=1253"}],"version-history":[{"count":0,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1253\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=1253"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=1253"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=1253"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}