{"id":1245,"date":"2019-12-08T22:17:32","date_gmt":"2019-12-08T14:16:42","guid":{"rendered":"http:\/\/haokebio.com\/1245.html"},"modified":"2019-12-08T22:16:42","modified_gmt":"2019-12-08T14:16:42","slug":"cc27d61d5f","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1245.html","title":{"rendered":"\u8d28\u7c92\u5927\u63d0\uff08\u7518\u6cb9\u83cc\uff09"},"content":{"rendered":"
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\u3000\u3000\u7b80\u4ecb\uff1a<\/strong><\/p>\n

\u3000\u3000\u8d28\u7c92\u8f6c\u5316\uff1a<\/strong>\u643a\u5e26\u5916\u6e90DNA\u7247\u6bb5\u7684\u8d28\u7c92\u8fdb\u5165\u611f\u53d7\u4f53\u7ec6\u80de\u540e\uff0c\u5728\u7ec6\u80de\u4e2d\u8fdb\u884c\u81ea\u6211\u590d\u5236\u3002\u591a\u7528\u4e8e\u540e\u671f\u63d0\u53d6\u8d28\u7c92\u3002<\/p>\n

\u3000\u3000\u8d28\u7c92\u63d0\u53d6\uff1a<\/strong>\u5373\u53bb\u9664 RNA\uff0c\u5c06\u8d28\u7c92\u4e0e\u7ec6\u83cc\u57fa\u56e0\u7ec4DNA\u5206\u5f00\uff0c\u53bb\u9664\u86cb\u767d\u8d28\u53ca\u5176\u5b83\u6742\u8d28\uff0c\u4ee5\u5f97\u5230\u76f8\u5bf9\u7eaf\u51c0\u7684\u8d28\u7c92\u3002\u76ee\u524d\u5b9e\u9a8c\u5ba4\u6d89\u53ca\u8f83\u591a\u7684\u4e3a\u5927\u91cf\u63d0\u53d6\u548c\u5c0f\u91cf\u63d0\u53d6\u3002<\/p>\n

\u3000\u3000\u5b9e\u9a8c\u6b65\u9aa4\uff1a<\/strong>(\u4ee5\u8d28\u7c92\u6837\u672c\u63d0\u53d6\u5927\u91cf\u8d28\u7c92\u4e3a\u4f8b)<\/p>\n

\u3000\u30001. \u4ece-80\u2103\u51b0\u7bb1\u4e2d\u53d6\u51fa\u5bf9\u5e94\u7684\u611f\u53d7\u6001\u7ec6\u80de\uff0c\u5ba4\u6e29\u4e0b\u7f6e\u4e8e\u51b0\u76d2\u4e0a\u89e3\u51bb\uff0c\u6807\u8bb0;<\/p>\n

\u3000\u30002. \u5411\u611f\u53d7\u6001\u7ec6\u80de\u4e2d\u52a0\u51652µl\u7684\u8d28\u7c92\uff0c\u7f6e\u4e8e4\u2103\u51b0\u7bb1\u4e2d,\u51b0\u6d7430min;<\/p>\n

\u3000\u30003. \u572842\u2103\u6c34\u6d74\u9505\u4e2d\u70ed\u6fc090s\uff0c\u7136\u540e\u7acb\u5373\u5728\u51b0\u76d2\u4e0a\u653e\u7f6e2min;<\/p>\n

\u3000\u30004. \u6bcf\u7ba1\u52a01mL LB\u57f9\u517b\u57fa\uff0c37\u2103\u6447\u5e8a\u9707\u8361,220rpm×30min;<\/p>\n

\u3000\u30005. \u53d6100µl \u8f6c\u5316\u6db2\u52a0\u5165\u5bf9\u5e94\u6297\u6027\u7684\u5e73\u677f\u4e2d\uff0c\u5012\u5165\u9002\u91cf\u73bb\u7483\u73e0\uff0c\u6d82\u5300\u6db2\u4f53;<\/p>\n

\u3000\u30006. \u5c06\u5e73\u677f\u5012\u7f6e\u4e8e37\u2103\u6052\u6e29\u57f9\u517b\u7bb1\u4e2d\u57f9\u517b\u8fc7\u591c\u3002<\/p>\n

\u3000\u30007. \u6311\u9009\u514b\u9686\u5b50\u63a5\u79cd\u4e8e1mL\u542b\u5bf9\u5e94\u6297\u6027\u7684LB\u6db2\u4f53\u57f9\u517b\u57fa\uff0c37\u2103\uff0c220rpm\u632f\u8361\u57f9\u517b\u8fc7\u591c\u3002<\/p>\n

\u3000\u30008. \u63091\/1000\u7684\u6bd4\u4f8b\uff0c\u63a5\u79cd\u4e8e200mL\u5bf9\u5e94\u6297\u6027\u7684LB\u6db2\u4f53\u57f9\u517b\u57fa\uff0c37\u2103\uff0c220rpm\u632f\u8361\u57f9\u517b\u8fc7\u591c\u3002<\/p>\n

\u3000\u30009. 4\u2103\u4e0b8000rpm\u79bb\u5fc3\u6536\u96c6\u83cc\u4f53\uff0c\u7528\u65e0\u5185\u6bd2\u7d20\u8d28\u7c92\u5927\u63d0\u8bd5\u5242\u76d2\u63d0\u53d6\u8d28\u7c92\u3002<\/p>\n

\u3000\u3000\u5b9e\u9a8c\u5468\u671f\uff1a<\/strong>7\u4e2a\u5de5\u4f5c\u65e5<\/p>\n

\u3000\u3000\u5b9e\u9a8c\u8981\u6c42\uff1a<\/strong>\u7518\u6cb9\u83cc50ul;\u8d28\u7c92\u6837\u54c1\uff0c20ng\/ul\uff0c20ul\u3002\u5e72\u51b0\u8fd0\u8f93\u3002<\/p>\n

\u3000\u3000\u6ce8\u660e\u7518\u6cb9\u83cc\u548c\u8d28\u7c92\u7684\u6297\u6027\u3002<\/p>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u3000\u3000\u7b80\u4ecb\uff1a \u3000\u3000\u8d28\u7c92\u8f6c\u5316\uff1a\u643a\u5e26\u5916\u6e90DNA\u7247\u6bb5\u7684\u8d28\u7c92\u8fdb\u5165\u611f\u53d7\u4f53\u7ec6\u80de\u540e\uff0c\u5728\u7ec6\u80de\u4e2d\u8fdb\u884c\u81ea\u6211\u590d\u5236\u3002\u591a\u7528\u4e8e\u540e\u671f\u63d0\u53d6\u8d28\u7c92\u3002 […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1245","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["2104"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1245"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=1245"}],"version-history":[{"count":0,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1245\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=1245"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=1245"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=1245"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}