{"id":1237,"date":"2019-12-08T22:17:24","date_gmt":"2019-12-08T14:16:34","guid":{"rendered":"http:\/\/haokebio.com\/1237.html"},"modified":"2019-12-08T22:16:34","modified_gmt":"2019-12-08T14:16:34","slug":"f97c905059","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1237.html","title":{"rendered":"\u7532\u57fa\u5316\u68c0\u6d4bMSP"},"content":{"rendered":"
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\"\u7532\u57fa\u5316\u68c0\u6d4bMSP\"<\/p>\n

\u3000\u3000\u6807\u672c\u8981\u6c42\uff1a<\/strong>\u7ec4\u7ec7\u3001\u7ec6\u80de\u3001<\/p>\n

\u3000\u3000\u5b9e\u9a8c\u5468\u671f\uff1a<\/strong>1-2\u5468<\/p>\n

\u3000\u3000\u6536\u8d39\u4ef7\u683c\uff1a<\/strong>\u6bcf\u4e2a\u6837\u54c1\u6bcf\u4e2a\u57fa\u56e0250\u5143\uff0c\u6bcf\u4e2a\u57fa\u56e02\u5bf9\u5f15\u7269\u8d392*70\u5143<\/p>\n

\u3000\u3000\u4ea7\u54c1\u4ecb\u7ecd\uff1a<\/strong>\u4e9a\u786b\u9178\u6c22\u76d0\u5904\u7406\u540e\u6d4b\u5e8f(bisulfite sequencing PCR,BSP)\u6cd5\u662f\u68c0\u6d4b\u57fa\u56e0\u7532\u57fa\u5316\u7684\u7ecf\u5178\u65b9\u6cd5,\u5176\u539f\u7406\u4e3a:\u7528\u4e9a\u786b\u9178\u6c22\u94a0\u4fee\u9970\u5904\u7406\u57fa\u56e0\u7ec4DNA,\u6240\u6709\u672a\u53d1\u751f\u7532\u57fa\u5316\u7684\u80de\u5bc6\u5576(c)\u88ab\u8f6c\u5316\u4e3a\u5c3f\u5bc6\u5576(U)\u800c\u7532\u57fa\u5316\u7684\u80de\u5627\u5576\u5219\u4e0d\u53d8\u3002\u636e\u6b64\u8bbe\u8ba1\u9488\u5bf9\u7532\u57fa\u5316\u548c\u975e\u7532\u57fa\u5316\u5e8f\u5217\u7684\u5f15\u7269\u8fdb\u884cPCR\u3002\u901a\u8fc7\u7535\u6cf3\u68c0\u6d4bMSP\u6269\u589e\u4ea7\u7269,\u5982\u679c\u7528\u9488\u5bf9\u5904\u7406\u540e\u7532\u57fa\u5316DNA\u94fe\u7684\u5f15\u7269\u80fd\u5f97\u5230\u6269\u589e\u7247\u6bb5,\u5219\u8bf4\u660e\u8be5\u4f4d\u70b9\u5b58\u5728\u7532\u57fa\u5316\u53cd\u4e4b,\u8bf4\u660e\u88ab\u68c0\u6d4b\u7684\u4f4d\u70b9\u4e0d\u5b58\u5728\u7532\u57fa\u5316\u3002\u82e5\u4e24\u961f\u5f15\u7269\u5747\u80fd\u5f97\u5230\u9633\u6027PCR\u4ea7\u7269,\u5219\u8bf4\u660e\u8be5\u7247\u6bb5\u90e8\u5206\u7532\u57fa\u5316\u3002<\/p>\n

\u3000\u3000\u5b9e\u9a8c\u6d41\u7a0b\uff1a<\/strong><\/p>\n

\u3000\u3000(1) \u6837\u672c\u63a5\u6536:\u7ec4\u7ec7\u3001\u7ec6\u80de\u3001\u8840\u6db2\u3001\u5176\u5b83<\/p>\n

\u3000\u3000(2) \u5b9e\u9a8c\u6b65\u9aa4: DNA\u63d0\u53d6-\u4e9a\u786b\u9178\u6c22\u76d0\u5904\u7406-\u7eaf\u5316DNA-PCR\u6269\u589e-\u7535\u6cf3<\/p>\n

\u3000\u3000(3)\u5b9e\u9a8c\u7ed3\u679c:\u5b8c\u6574\u9879\u76ee\u62a5\u544a(\u6750\u6599\u3001\u8bd5\u5242\u3001\u4eea\u5668\u3001\u65b9\u6cd5\u3001\u6570\u636e\u5206\u6790\u3001\u5b9e\u9a8c\u7ed3\u679c)<\/p>\n

\u3000\u3000\u6ce8\u610f\u4e8b\u9879:<\/strong><\/p>\n

\u3000\u30001\u3001\u7ec4\u7ec7\u6837\u54c1:\u65b0\u9c9c\u7ec4\u7ec7\u653e\u5165\u6db2\u6c2e\u6216-80\u5ea6\u4fdd\u5b58,\u7ec4\u7ec7\u4e0d\u5c11\u4e8e100mg,<\/p>\n

\u3000\u30002\u3001\u57f9\u517b\u7ec6\u80de:\u901a\u8fc7\u7ec6\u80de\u8ba1\u6570\u53d6\u4e0d\u5c11\u4e8e2×106<\/sup>\u4e2a\u7ec6\u80de\u4e8eEP\u7ba1,\u6536\u96c6\u7ec6\u80de\u6c89\u6dc0\u540e,\u6db2\u6c2e\u901f\u51bb,\u4f4e\u6e29\u4fdd\u5b58\u6216\u8fd0\u8f93(-80\u2103,\u907f\u514d\u53cd\u590d\u51bb\u878d);<\/p>\n

\u3000\u30003\u3001\u8840\u6db2\u6807\u672c:\u4ee5\u6297\u51dd\u7ba1\u4fdd\u5b58\u8840\u6837(-4\u2103\u4fdd\u5b583~5\u5929,\u5982\u679c\u8981\u957f\u65f6\u95f4\u4fdd\u5b58\u9700\u653e\u5728-80\u2103);<\/p>\n

\u3000\u30004\u3001DNA\u6837\u54c1\u9700\u6807\u660e\u6d53\u5ea6\u548c\u7eaf\u5ea6(\u4f53\u79ef≥50u,\u6d53\u5ea6≥ 50 ng\/ul);<\/p>\n

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