{"id":1232,"date":"2019-12-08T22:17:17","date_gmt":"2019-12-08T14:16:27","guid":{"rendered":"http:\/\/haokebio.com\/1232.html"},"modified":"2019-12-08T22:16:27","modified_gmt":"2019-12-08T14:16:27","slug":"bdceb1c253","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1232.html","title":{"rendered":"PCR\u4ea7\u7269\u7535\u6cf3"},"content":{"rendered":"
\n
\n

\u3000\u3000\u7b80\u4ecb\uff1a<\/strong>\u8367\u5149\u5b9a\u91cfPCR\u662f\u901a\u8fc7\u8367\u5149\u67d3\u6599(SYBR Green)\u6216\u8367\u5149\u6807\u8bb0\u7684\u7279\u5f02\u6027\u7684\u63a2\u9488\uff0c\u5bf9PCR\u4ea7\u7269\u8fdb\u884c\u6807\u8bb0\u8ddf\u8e2a\uff0c\u5b9e\u65f6\u5728\u7ebf\u76d1\u63a7\u53cd\u5e94\u8fc7\u7a0b\uff0c\u7ed3\u5408\u76f8\u5e94\u7684\u8f6f\u4ef6\u53ef\u4ee5\u5bf9\u4ea7\u7269\u8fdb\u884c\u5206\u6790\uff0c\u8ba1\u7b97\u5f85\u6d4b\u6837\u54c1\u6a21\u677f\u7684\u521d\u59cb\u6d53\u5ea6\u3002\u53ef\u4ee5\u7528\u4e8e\u68c0\u6d4bmRNA\u3001lncRNA\u3001circRNA\u3001miRNA\u7684\u57fa\u56e0\u8868\u8fbe\u6c34\u5e73\u3002\u6700\u7ec8\u6240\u5f97\u7684PCR\u4ea7\u7269\u53ef\u4ee5\u901a\u8fc7\u743c\u8102\u7cd6\u51dd\u80f6\u7535\u6cf3\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u5f15\u7269\u7279\u5f02\u6027\u3002<\/p>\n

\u3000\u3000\u5b9e\u9a8c\u6d41\u7a0b\uff1a<\/strong><\/p>\n

\u3000\u30001.\u63a5\u6536\u6837\u672c<\/p>\n

\u3000\u30002.\u7ec4\u7ec7\u5300\u6d46\u3001\u7ec6\u80de\u548c\u8840\u6db2\u6837\u672c\u5439\u6253\u88c2\u89e3-RNA\u63d0\u53d6-RNA\u6d53\u5ea6\u6d4b\u5b9a—\u9006\u8f6c\u5f55\u6210cdna—\u8367\u5149\u5b9a\u91cfPCR-(PCR\u4ea7\u7269\u7535\u6cf3)-\u6570\u636e\u5206\u6790<\/p>\n

\u3000\u3000\u5176\u4e2dmRNA\u4e3a\u666e\u901a\u9006\u8f6c\u5f55\uff0clncRNA\u53ef\u9009\u91c7\u53d6\u666e\u901a\u548c\u7279\u5f02\u6027\u9006\u8f6c\u5f55\uff0ccircRNA\u548cmiRNA\u91c7\u53d6\u7279\u5f02\u6027\u9006\u8f6c\u5f55<\/p>\n

\u3000\u30003.\u4ea4\u4ed8\u7ed3\u679c\uff1a\u5b8c\u6574\u9879\u76ee\u62a5\u544a(\u6269\u589e\u66f2\u7ebf\u3001\u6570\u636e\u5206\u6790\u3001\u5f15\u7269\u4fe1\u606f\u3001\u5b9e\u9a8c\u62a5\u544a)<\/p>\n

\u3000\u3000\u5b9e\u9a8c\u5468\u671f\uff1a<\/strong>60\u4e2a\u5faa\u73af\u5185\uff0c7\u4e2a\u5de5\u4f5c\u65e5\u5185\u51fa\u7ed3\u679c<\/p>\n

\u3000\u3000\u6837\u54c1\u8981\u6c42\uff1a<\/strong><\/p>\n

\u3000\u30001\u3001\u7ec4\u7ec7\uff1a 100mg(\u9ec4\u8c46\u5927\u5c0f)\uff0c\u6700\u5c1150mg(\u7eff\u8c46\u5927\u5c0f)\uff0c\u8102\u80aa\u7ec4\u7ec7\uff0c\u7ed3\u7f14\u7ec4\u7ec7\uff0c\u7ea4\u7ef4\u5316\u7ec4\u7ec7\u9002\u5f53\u589e\u52a0\u3002<\/p>\n

\u3000\u30002\u3001\u7ec6\u80de\uff1a1×10^6\u4e2a\u7ec6\u80de,\u8d34\u58c1\u7ec6\u80de\u8212\u5c55\uff0c\u672a\u53d8\u5706;<\/p>\n

\u3000\u30003\u3001\u5168\u8840\uff1a2mL\uff0c\u6700\u5c111mL\u65b0\u9c9c\u8840\u6db2EDTA\u6297\u51dd\u7ba1;<\/p>\n

\u3000\u30004\u3001\u8840\u6e05\uff1a2mL\uff0c\u5e72\u51b0\u8fd0\u8f93;<\/p>\n

\u3000\u30005\u3001\u690d\u7269\uff1a\u53f6\u3001\u679c\u8089\u3001\u79cd\u5b50\u7b49\u9700100mg\uff0c\u6700\u5c1150mg\u3002<\/p>\n

\u3000\u30006\u3001RNA\u6837\u54c1\uff1a\u7eaf\u5ea6OD260\/280 \u6bd4\u503c\u4e3a1.8-2.0\uff0c\u6d53\u5ea6≥500ng\/ul\uff0c\u4f53\u79ef≥20ul\u3002<\/p>\n

\u3000\u30007\u3001cdna\uff1acdna\u539f\u6db220uL\u3002<\/p>\n

\u3000\u3000\u6837\u672c\u8fd0\u8f93\uff1a<\/strong>\u53ef\u9009\u62e9\u4ee5\u4e0b\u4efb\u4f55\u4e00\u79cd\u65b9\u5f0f\u5bc4\u9001\u6807\u672c<\/p>\n

\u3000\u30001.\u52a8\u690d\u7269\u7ec4\u7ec7\u6837\u672c\u3001\u7ec6\u80de\u6837\u672c\u3001\u8840\u6e05\u3001RNA\u3001cDNA\u4ee5\u5e72\u51b0\u8fd0\u8f93;<\/p>\n

\u3000\u30002.\u7ec6\u80de\u6837\u672c\u4e5f\u53ef\u76f4\u63a5\u5bc4\u9001\u57f9\u517b\u74f6(\u5bc6\u5c01\u4fdd\u8bc1\u4e0d\u6c61\u67d3\u3001\u704c\u6ee1\u57f9\u517b\u6db2\u4e0d\u6f0f\u51fa)\uff0c\u5e38\u6e29\u8fd0\u8f93;<\/p>\n

\u3000\u30003.\u5168\u8840\u6837\u672c\u662fEDTA\u6297\u51dd\u7ba14\u5ea6\u8fd0\u8f93<\/p>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u3000\u3000\u7b80\u4ecb\uff1a\u8367\u5149\u5b9a\u91cfPCR\u662f\u901a\u8fc7\u8367\u5149\u67d3\u6599(SYBR Green)\u6216\u8367\u5149\u6807\u8bb0\u7684\u7279\u5f02\u6027\u7684\u63a2\u9488\uff0c\u5bf9PCR\u4ea7\u7269\u8fdb\u884c\u6807\u8bb0\u8ddf […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1232","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["777"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1232"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=1232"}],"version-history":[{"count":0,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1232\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=1232"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=1232"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=1232"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}