{"id":1205,"date":"2019-12-08T22:16:59","date_gmt":"2019-12-08T14:16:09","guid":{"rendered":"http:\/\/haokebio.com\/1205.html"},"modified":"2019-12-08T22:16:10","modified_gmt":"2019-12-08T14:16:10","slug":"94cffda3e4","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1205.html","title":{"rendered":"\u5206\u5b50\u751f\u7269\u5b66\u68c0\u6d4b"},"content":{"rendered":"
\n
\n

\u3000\u3000\u9879\u76ee\u4ecb\u7ecd<\/strong><\/p>\n

\u3000\u3000\u8367\u5149\u5b9a\u91cfPCR<\/strong><\/p>\n

\u3000\u3000\u8367\u5149\u5b9a\u91cfPCR \u662f\u901a\u8fc7\u8367\u5149\u67d3\u6599(SYBR Green) \u6216\u8367\u5149\u6807\u8bb0\u7684\u7279\u5f02\u6027\u7684\u63a2\u9488\uff0c\u5bf9PCR \u4ea7\u7269\u8fdb\u884c\u6807\u8bb0\u8ddf\u8e2a\uff0c\u5b9e\u65f6\u5728\u7ebf\u76d1\u63a7\u53cd\u5e94\u8fc7\u7a0b\uff0c\u7ed3\u5408\u76f8\u5e94\u7684\u8f6f\u4ef6\u53ef\u4ee5\u5bf9\u4ea7\u7269\u8fdb\u884c\u5206\u6790\uff0c\u8ba1\u7b97\u5f85\u6d4b\u6837\u54c1\u6a21\u677f\u7684\u521d\u59cb\u6d53\u5ea6\u3002<\/p>\n

 <\/p>\n

\u3000\u3000\u7532\u57fa\u5316\u68c0\u6d4b<\/strong><\/p>\n

\u3000\u3000DNA \u7532\u57fa\u5316\u662f\u5728DNA \u7532\u57fa\u5316\u8f6c\u79fb\u9176(DNMTs) \u7684\u4f5c\u7528\u4e0b\u4f7fCpG \u4e8c\u6838\u9996\u91785'\u7aef\u7684\u80de\u55dc\u5517\u8f6c\u53d8\u4e3a5'- \u7532\u57fa\u80de\u5565\u5517\u3002DNA \u7532\u57fa\u5316\u901a\u5e38\u6291\u5236\u57fa\u56e0\u8868\u8fbe\uff0c\u53bb\u7532\u57fa\u5316\u5219\u8bf1\u5bfc\u4e86\u57fa\u56e0\u7684\u91cd\u65b0\u6d3b\u5316\u548c\u8868\u8fbe\u3002<\/p>\n

 <\/p>\n

\u3000\u3000\u7ed3\u679c\u5c55\u793a<\/strong><\/p>\n

 <\/p>\n

\"\u5206\u5b50\u751f\u7269\u5b66\u68c0\u6d4b\"<\/p>\n

\"\u5206\u5b50\u751f\u7269\u5b66\u68c0\u6d4b\"<\/p>\n

\u3000\u3000\u9879\u76ee\u4ef7\u683c<\/strong><\/p>\n

\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u670d\u52a1\u9879\u76ee<\/strong><\/td>\n\u53d6\u6837\u8981\u6c42<\/strong><\/td>\n\u4fdd\u5b58\u6761\u4ef6<\/strong><\/td>\n\u62a5\u544a\u5f62\u5f0f<\/strong><\/td>\n\u4ef7\u683c\uff08\u5143\uff09<\/strong><\/td>\n<\/tr>\n
\u666e\u901a PCR\uff08\u7535\u6cf3\uff09<\/td>\n 
 
 
1\u3001\u7ec4\u7ec7
—\u822c 100mg (\u9ec4\u8c46\u5927\u5c0f\uff09\uff0c\u6700\u5c11\u4e5f\u987b 50mg (\u7eff\u8c46\u5927\u5c0f\uff09\uff0c\u8102\u80aa\u7ec4\u7ec7\uff0c \u7ed3\u7f14\u7ec4\u7ec7\uff0c\u7ea4\u7ef4\u5316\u7ec4\u7ec7\u9002\u5f53\u589e\u52a0\u3002 2\u3001\u7ec6\u80de
1X 106<\/sup> \u4e2a\u7ec6\u80de \uff0c\u8d34\u58c1\u7ec6\u80de \u8212\u5c55\uff0c \u672a\u53d8\u5706\uff1b\u60ac\u6d6e\u7ec6\u80de\u5f62\u6001\u5b8c\u6574\uff0c\u900f\u4eae\u3002 
3\u3001\u8840\u6db2
2mL, \u6700\u5c11 1mL \u65b0\u9c9c\u8840\u6db2 EDTA \u6297\u51dd\uff0c \u6700\u597d\u662f\u5c3d\u5feb\u7528\u7ea2\u7ec6\u80de\u88c2\u89e3\u6db2\u5206\u79bb\uff0c \u5145\u5206\u53bb\u9664\u7ea2\u7ec6\u80de\uff0c\u4fdd\u7559\u6709\u6838\u7ec6\u80de\uff0c \u7136\u540e\u52a0\u5165 trizol \u653e\u5165 -80 \u5ea6\u4fdd\u5b58\u3002<\/td>\n		 
1\u3001\u7ec4\u7ec7
\u65b0\u9c9c\u7ec4\u7ec7\u7acb\u5373\u653e\u4eba \u6c2e\u4e2d\uff0c\u51b0\u51bb\u7684\u7ec4\u7ec7 \u53ef\u76f4\u63a5\u653e\u5165-80°C \u50a8\u5b58\uff0c\u5e72\u51b0\u8fd0\u8f93\u3002
2\u3001\u7ec6\u80de
\u5e26\u57f9\u517b\u57fa\u5e38\u6e29\u8fd0\u8f93\uff0c \u6216\u8005\u63d0\u4f9b\u7ec6\u80de\u6c89\u6dc0\u5e72\u51b0\u8fd0\u8f93\u3002
3 \u3001 \u8840\u6db2EDTA \u6297\u51dd\u8840\u53ef\u4ee5 \u653e\u4e8e 4 \u5ea6 2-5 \u5929\u3002<\/td>\n		\u7535\u6cf3\u56fe\uff0c\u5f15\u7269\u4fe1\u606f\uff0c\u5b9e\u9a8c\u6761\u4ef6<\/td>\n\u7b2c—\u4e2a\u57fa\u56e0 100 \u5143\uff0f\u6837\u672c
\u7b2c\u4e8c\u4e2a\u57fa\u56e0 50 \u5143\uff0f\u6837\u672c<\/td>\n<\/tr>\n
\u8367\u5149\u5b9a\u91cf PCR<\/td>\n\u6269\u589e\u66f2\u7ebf\uff0c\u5faa\u73af\u9605\u503c\uff0c\u6570\u636e\u5206\u6790\uff0c\u5f15\u7269\u4fe1\u606f\uff0c\u5b9e\u9a8c\u6761\u4ef6<\/td>\n\u7b2c—\u4e2a\u57fa\u56e0 120 \u5143\uff0f\u6837\u672c
\u7b2c\u4e8c\u4e2a\u57fa\u56e0 60 \u5143\uff0f\u6837\u672c<\/td>\n<\/tr>\n
miRNA \u68c0\u6d4b<\/td>\n200 \u5143\uff0f\u6837\u672c\uff0f\u57fa\u56e0<\/td>\n<\/tr>\n
 
DNA \u5b9a\u91cf<\/td>\n		\u6d53\u5ea6\u4fe1\u606f\uff0c\u6570\u636e\u5206\u6790\uff0c\u5b9e\u9a8c\u6761\u4ef6<\/td>\n 
100 \u5143\uff0f\u6837<\/td>\n<\/tr>\n
 
DNA ladder<\/td>\n		\u7535\u6cf3\u56fe\uff0c\u6d53\u5ea6\u4fe1\u606f\uff0c\u6570\u636e\u5206\u6790\uff0c\u5b9e\u9a8c\u6761\u4ef6<\/td>\n 
100 \u5143\uff0f\u6837<\/td>\n<\/tr>\n
 \u7532\u57fa\u5316\u68c0\u6d4b<\/td>\n 
\u7535\u6cf3\u56fe (MSP)\/ \u6d4b\u5e8f\u7ed3\u679c(BSP), \u5f15\u7269\u4fe1\u606f\uff0c\u5b9e\u9a8c\u6761\u4ef6<\/td>\n		MSP 250 \u5143\uff0f\u6837\u672c
\u5f15\u7269 2*70 \u5143
BSP 800 \u5143\uff0f\u6837\u672c
\u5f15\u7269 70 \u5143<\/td>\n<\/tr>\n
 \u8d28\u7c92\u63d0\u53d6<\/td>\n -80\u2103\u50a8\u5b58\uff0c\u5e72\u51b0\u8fd0\u8f93\u3002<\/td>\n \u8d28\u7c92\uff0c \u83cc\u6db2\uff0c \u5b9e\u9a8c\u6761\u4ef6<\/td>\n500 \u5143\uff0f\u4e2a \u83cc\u6db2
800 \u5143\uff0f\u4e2a \u8d28\u7c92<\/td>\n<\/tr>\n
\u8d28\u7c92\u6784\u5efa<\/td>\n\u8d28\u7c92\uff0c\u6d4b\u5e8f\u62a5\u544a<\/td>\n2000 \u5143\u8d77<\/td>\n<\/tr>\n
 \u83cc\u79cd\u9274\u5b9a<\/td>\n \u5df2\u5206\u79bb\u51fa\u7684\u7eaf\u7ec6\u83cc\u6c89\u6dc0\uff08\u7eff\u8c46\u5927\u5c0f\uff09<\/td>\n 
\u5e72\u51b0<\/td>\n		\u6d53\u5ea6\u4fe1\u606f\uff0c\u7535\u6cf3\u56fe\uff0c\u6d4b\u5e8f\u7ed3\u679c\uff08\u5305\u62ec\u62fc\u63a5\u7ed3 \u679c\uff09\uff0c\u6570\u636e\u5206\u6790\uff0c\u5b9e\u9a8c\u6761\u4ef6<\/td>\n 300 \u5143\uff0f\u6837<\/td>\n<\/tr>\n
 \u5f57\u661f\u5b9e\u9a8c<\/td>\n \u6d3b\u7ec6\u80de\uff0c 1X l07<\/sup>\u4e2a\u7ec6\u80de\uff0c \u8d34\u58c1\u7ec6\u80de\u8212\u5c55\uff0c\u672a\u53d8\u5706\u3002<\/td>\n 4 \u5ea6<\/td>\n\u7167\u7247 5 \u5f20 (200X), \u6570\u636e\u5206\u6790
(30 \u4e2a\u6709\u6548\u7ec6\u80de\u7684\u6570\u636e\uff09\uff0c \u5b9e\u9a8c\u6761\u4ef6<\/td>\n		 1000 \u5143\uff0f\u6837<\/td>\n<\/tr>\n<\/tbody>\n<\/table><\/div>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u3000\u3000\u9879\u76ee\u4ecb\u7ecd \u3000\u3000\u8367\u5149\u5b9a\u91cfPCR \u3000\u3000\u8367\u5149\u5b9a\u91cfPCR \u662f\u901a\u8fc7\u8367\u5149\u67d3\u6599(SYBR Green) \u6216\u8367\u5149\u6807\u8bb0\u7684\u7279\u5f02 […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1205","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["1072"],"_edit_lock":["1729817471:2"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1205"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=1205"}],"version-history":[{"count":0,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1205\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=1205"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=1205"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=1205"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}