![\"RIPA](\"https:\/\/www.haokebio.com\/wp-content\/uploads\/2024\/05\/444.jpg?imageView2\/0\/format\/webp\/q\/75\")
<\/a><\/div><\/div>RIPA \u603b\u86cb\u767d\u88c2\u89e3\u6db2\uff08\u4e2d\uff09\n\n<\/h1><\/div><\/i>\u86cb\u767d\u8d28\u63d0\u53d6\u4e0e\u5b9a\u91cf<\/a> \u86cb\u767d\u8d28\u751f\u7269\u5b66<\/a> \u8bd5\u5242<\/a> <\/span><\/div><\/header>- \u4ef7\u683c<\/span>\u00a5120<\/span><\/li>
- \u89c4\u683c<\/span>100ml<\/span><\/li>
- \u8d27\u53f7<\/span>HKW2011B<\/span><\/li>
- \u5355\u4f4d<\/span>\u74f6<\/span><\/li>
- \u54c1\u724c<\/span>HaoKebio<\/span><\/li><\/ul>\n\n\n\nQQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n
<\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>
<\/div><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div>\n\n<\/p>\n\n\n\n
\n\n<\/p>\n<\/div>\n<\/div>\n\n\n\n
<\/p>\n\n\n\n
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\n\u6682\u65e0<\/p>\n\n\n\n
\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3>- <\/i><\/a>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><\/div><\/section>\n\n\n\n
<\/p>\n<\/div>\n\n\n\n
<\/div>\n<\/div><\/div><\/div><\/div>\n\n\n\n\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><\/strong><\/p>\n\n\n\n
RIPA \u88c2\u89e3\u6db2\uff08RIPA Lysis Buffer\uff09\u662f\u4e00\u79cd\u4f20\u7edf\u7684\u7ec6\u80de\u53ca\u7ec4\u7ec7\u5feb\u901f\u88c2\u89e3\u6db2\u3002RIPA \u88c2\u89e3\u6db2\u6709\u5f88\u591a\u914d\u65b9\uff0c\u6309\u5176\u88c2\u89e3\u6548\u679c\u4e3b\u8981\u5206\u4e3a\u5f3a\uff0c\u4e2d\uff0c\u5f31\u4e09\u79cd\u3002RIPA \u5f3a\u89e3\u6db2\u88c2\u89e3\u7ec4\u7ec7\u3001\u7ec6\u80de\u5f97\u5230\u7684\u86cb\u767d\u6837\u54c1\u53ef\u4ee5\u7528\u4e8e\u5e38\u89c4\u7684 PAGE\u3001Western \u7b49\u5bf9\u86cb\u767d\u6d3b\u6027\u6ca1\u6709\u4e25\u683c\u8981\u6c42\u7684\u5b9e\u9a8c\u3002\u672c\u4ea7\u54c1\u4e3b\u8981\u6210\u5206\u5305\u542b 50 mM Tris-HCl (pH 7.4)\uff0c150 mM NaCl\uff0c1 mM EDTA-2Na\uff0c1% Triton X-100\uff0c1%\u8131\u6c27\u80c6\u9178\u94a0\u53ca 0.1% SDS\u3002<\/p>\n\n\n\n
\u672c\u4ea7\u54c1\u9002\u7528\u4e8e\u52a8\u7269\u6216\u690d\u7269\u7ec4\u7ec7\u53ca\u7ec6\u80de\u6837\u54c1\uff0c\u4e5f\u53ef\u7528\u4e8e\u771f\u83cc\u6216\u7ec6\u83cc\u6837\u54c1\u3002<\/p>\n\n\n\n
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\u3010\u4ea7\u54c1\u7ec4\u6210 \u3011<\/strong><\/p>\n\n\n\n
\u4ea7\u54c1\u76ee\u5f55<\/td> \u4e3b\u8981\u6210\u5206<\/td> \u4ea7\u54c1\u89c4\u683c<\/td><\/tr> RIPA\u00a0\u603b\u86cb\u767d\u88c2\u89e3\u6db2\uff08\u4e2d\uff09<\/td> \u8131\u6c27\u80c6\u9178\u94a0<\/td> 100mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n\u3010<\/strong>\u50a8\u5b58\u4e0e\u8fd0\u8f93<\/strong>\u3011<\/strong><\/strong><\/p>\n\n\n\n
\u51b0\u888b\uff08wet ice\uff09\u8fd0\u8f93\uff1b4\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u6709\u6548\u671f 18 \u4e2a\u6708\u3002<\/p>\n\n\n\n
\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><\/strong><\/p>\n\n\n\n
\u81ea\u5907\u86cb\u767d\u9176\u6291\u5236\u5242\u3002RIPA \u88c2\u89e3\u6db2\uff08\u5f3a\uff09\u5728\u4e34\u7528\u524d\u9700\u52a0\u5165\u86cb\u767d\u9176\u6291\u5236\u5242\uff0c\u9632\u6b62\u86cb\u767d\u964d\u89e3\u3002<\/p>\n\n\n\n
\u4ee5\u4e0b\u4f7f\u7528\u65b9\u6cd5\u4e2d\u63d0\u5230\u7684 RIPA \u88c2\u89e3\u6db2(\u5f3a)\u5747\u6307\u5df2\u6dfb\u52a0\u86cb\u767d\u9176\u6291\u5236\u5242\u3002<\/p>\n\n\n\n
\u7ec4\u7ec7\u6837\u54c1<\/strong>\u5b9e\u9a8c<\/strong>\uff1a<\/p>\n\n\n\n
1. \u7ec4\u7ec7\u5757\u7528\u9884\u51b7 PBS\u6d17\u6da4\uff0c\u53bb\u9664\u8840\u6c61\uff0c\u526a\u6210\u7ec6\u5c0f\u788e\u5757\u7f6e\u4e8e\u5300\u6d46\u5668\u4e2d\u3002<\/p>\n\n\n\n
2. \u52a0\u5165 10 \u500d\u7ec4\u7ec7\u4f53\u79ef RIPA \u88c2\u89e3\u6db2(\u5f3a)\u4f4e\u6e29\u5300\u6d46\u6ce8\u610f\uff0cRIPA \u88c2\u89e3\u6db2(\u5f3a)\u7684\u4f7f\u7528\u91cf\u53ef\u6309\u7167\u7ea6\u6bcf 50 mg \u7ec4\u7ec7\u4e0e 1 mL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u6dfb\u52a0\u3002\u5982\u7ec4\u7ec7\u86cb\u767d\u542b\u91cf\u8f83\u4f4e\uff0c\u53ef\u964d\u4f4e\u88c2\u89e3\u6db2\u7684\u7528\u91cf\uff0c\u4ee5\u63d0\u9ad8\u7c97\u63d0\u6eb6\u6db2\u4e2d\u7684\u86cb\u767d\u6d53\u5ea6\u3002<\/p>\n\n\n\n
3. \u5c06\u5300\u6d46\u6db2\u8f6c\u79fb\u81f3 1.5 mL \u79bb\u5fc3\u7ba1\u4e2d\uff0c\u632f\u8361\u3002\u51b0\u6d74 30 min\uff0c\u671f\u95f4\u6bcf 10 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\uff0c\u786e\u4fdd\u7ec4\u7ec7\u7ec6\u80de\u5b8c\u5168\u88c2\u89e3\uff1b<\/p>\n\n\n\n
4. 12000g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/p>\n\n\n\n
\u5bf9\u4e8e\u8d34\u58c1\u7ec6\u80de\u6837\u54c1\uff1a<\/strong><\/strong><\/p>\n\n\n\n
1. \u7528 PBS \u6e05\u6d17\u7ec6\u80de 2-3 \u6b21\uff0c\u6700\u540e\u4e00\u6b21\u5f7b\u5e95\u5438\u5e72\u6b8b\u7559\u6db2\u3002<\/p>\n\n\n\n
2. \u6309\u7167 6 \u5b54\u677f\u6bcf\u5b54\u7ec6\u80de 250 \u03bcL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u5438\u53d6 RIPA \u88c2\u89e3\u6db2(\u5f3a)\u4e8e\u7ec6\u80de\u57f9\u517b\u677f\u3001\u74f6\u5185\uff0c\u53cd\u590d\u6643\u52a8\u57f9\u517b\u677f\u3001\u74f6\uff0c\u4f7f\u88c2\u89e3\u6db2\u4e0e\u7ec6\u80de\u5145\u5206\u63a5\u89e6 3-5 min\u3002<\/p>\n\n\n\n
3. \u7528\u7ec6\u80de\u522e\u5200\u5c06\u7ec6\u80de\u522e\u4e0b\uff0c\u6536\u96c6\u5230\u79bb\u5fc3\u7ba1\u4e2d\u3002<\/p>\n\n\n\n
4. 12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/p>\n\n\n\n
\n- <\/li>\n<\/ol>\n\n\n\n
\u5bf9\u4e8e\u60ac\u6d6e\u7ec6\u80de\u6837\u54c1\uff1a<\/strong><\/p>\n\n\n\n
\n- \u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\u3002<\/li>\n\n\n\n
- \u6309\u7167 6 \u5b54\u677f\u6bcf\u5b54\u7ec6\u80de 250 \u03bcL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u5c06\u7ec6\u80de\u6db2\u4e0e RIPA \u88c2\u89e3\u6db2(\u5f3a)\u6df7\u5408\uff0c\u632f\u8361\u3002<\/li>\n\n\n\n
- \u51b0\u6d74 30 min\uff0c\u671f\u95f4\u6bcf 10 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6570\u6b21\uff0c\u786e\u4fdd\u7ec6\u80de\u5b8c\u5168\u88c2\u89e3\u3002<\/li>\n\n\n\n
- 12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/li>\n<\/ol>\n\n\n\n
\u5bf9\u4e8e\u7ec6\u83cc\u6216\u771f\u83cc\u6837\u672c\uff1a<\/strong><\/p>\n\n\n\n
\n- \u53d6 1 mL \u83cc\u60ac\u6db2\uff0c\u79bb\u5fc3\u53bb\u4e0a\u6e05\uff0cPBS \u6d17\u6da4\u4e00\u6b21\uff0c\u5145\u5206\u53bb\u9664\u6db2\u4f53\u3002\u6da1\u65cb\u4f7f\u83cc\u4f53\u5c3d\u91cf\u5206\u6563\u3002<\/li>\n\n\n\n
- \u52a0\u5165 100-200 \u03bcL RIPA \u88c2\u89e3\u6db2(\u5f3a)\uff0c\u8f7b\u8f7b\u6da1\u65cb\u4f7f\u83cc\u4f53\u4e0e\u88c2\u89e3\u6db2\u5145\u5206\u6df7\u5300\u3002<\/li>\n\n\n\n
- \u51b0\u6d74 10 min\uff0c\u671f\u95f4\u6bcf 2 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6570\u6b21\uff0c\u786e\u4fdd\u83cc\u4f53\u5b8c\u5168\u88c2\u89e3\u3002<\/li>\n\n\n\n
- 12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/li>\n<\/ol>\n\n\n\n
\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><\/strong><\/p>\n\n\n\n
\n- \u7ec4\u7ec7\u6216\u7ec6\u80de\u88c2\u89e3\u65f6\u82e5\u4f1a\u51fa\u73b0\u7c98\u7a20\u72b6\u3002\u53ef\u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6216\u632f\u8361\u5668\u632f\u8361\uff0c\u76f4\u81f3\u5448\u6db2\u72b6\u4e3a\u6b62\u3002\u5982\u679c\u4e00\u76f4\u8f83\u7a20\uff0c\u53ef\u518d\u52a0\u5165\u9002\u91cf\u88c2\u89e3\u6db2\u3002<\/li>\n\n\n\n
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- \u4ef7\u683c<\/span>\u00a5120<\/span><\/li>
- \u89c4\u683c<\/span>100ml<\/span><\/li>
- \u8d27\u53f7<\/span>HKW2011B<\/span><\/li>
- \u5355\u4f4d<\/span>\u74f6<\/span><\/li>
- \u54c1\u724c<\/span>HaoKebio<\/span><\/li><\/ul>\n\n\n\nQQ53383090 \u54a8\u8be2\u8d2d\u4e70<\/a><\/div>\n<\/div>\n\n\n\n
<\/p>\n<\/div>\n<\/div><\/div>\n<\/div>\n<\/div>
<\/div><\/i>\u4ea7\u54c1\u4ecb\u7ecd<\/span><\/div><\/i>\u4ea7\u54c1\u53c2\u6570<\/span><\/div><\/i>\u8d44\u6599\u4e0b\u8f7d<\/span><\/div><\/div><\/div>\n\n<\/p>\n\n\n\n
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\n\u6682\u65e0<\/p>\n\n\n\n
\u8bf4\u660e\u6587\u6863\u4e0b\u8f7d<\/span><\/h3>
- <\/i><\/a>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul><\/div><\/section>\n\n\n\n
<\/p>\n<\/div>\n\n\n\n
<\/div>\n<\/div><\/div><\/div><\/div>\n\n\n\n\u3010\u4ea7\u54c1\u7b80\u4ecb\u3011<\/strong><\/strong><\/p>\n\n\n\n
RIPA \u88c2\u89e3\u6db2\uff08RIPA Lysis Buffer\uff09\u662f\u4e00\u79cd\u4f20\u7edf\u7684\u7ec6\u80de\u53ca\u7ec4\u7ec7\u5feb\u901f\u88c2\u89e3\u6db2\u3002RIPA \u88c2\u89e3\u6db2\u6709\u5f88\u591a\u914d\u65b9\uff0c\u6309\u5176\u88c2\u89e3\u6548\u679c\u4e3b\u8981\u5206\u4e3a\u5f3a\uff0c\u4e2d\uff0c\u5f31\u4e09\u79cd\u3002RIPA \u5f3a\u89e3\u6db2\u88c2\u89e3\u7ec4\u7ec7\u3001\u7ec6\u80de\u5f97\u5230\u7684\u86cb\u767d\u6837\u54c1\u53ef\u4ee5\u7528\u4e8e\u5e38\u89c4\u7684 PAGE\u3001Western \u7b49\u5bf9\u86cb\u767d\u6d3b\u6027\u6ca1\u6709\u4e25\u683c\u8981\u6c42\u7684\u5b9e\u9a8c\u3002\u672c\u4ea7\u54c1\u4e3b\u8981\u6210\u5206\u5305\u542b 50 mM Tris-HCl (pH 7.4)\uff0c150 mM NaCl\uff0c1 mM EDTA-2Na\uff0c1% Triton X-100\uff0c1%\u8131\u6c27\u80c6\u9178\u94a0\u53ca 0.1% SDS\u3002<\/p>\n\n\n\n
\u672c\u4ea7\u54c1\u9002\u7528\u4e8e\u52a8\u7269\u6216\u690d\u7269\u7ec4\u7ec7\u53ca\u7ec6\u80de\u6837\u54c1\uff0c\u4e5f\u53ef\u7528\u4e8e\u771f\u83cc\u6216\u7ec6\u83cc\u6837\u54c1\u3002<\/p>\n\n\n\n
<\/p>\n\n\n\n
\u3010\u4ea7\u54c1\u7ec4\u6210 \u3011<\/strong><\/p>\n\n\n\n
\u4ea7\u54c1\u76ee\u5f55<\/td> \u4e3b\u8981\u6210\u5206<\/td> \u4ea7\u54c1\u89c4\u683c<\/td><\/tr> RIPA\u00a0\u603b\u86cb\u767d\u88c2\u89e3\u6db2\uff08\u4e2d\uff09<\/td> \u8131\u6c27\u80c6\u9178\u94a0<\/td> 100mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n \u3010<\/strong>\u50a8\u5b58\u4e0e\u8fd0\u8f93<\/strong>\u3011<\/strong><\/strong><\/p>\n\n\n\n
\u51b0\u888b\uff08wet ice\uff09\u8fd0\u8f93\uff1b4\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u6709\u6548\u671f 18 \u4e2a\u6708\u3002<\/p>\n\n\n\n
\u3010\u4f7f\u7528\u65b9\u6cd5\u3011<\/strong><\/strong><\/p>\n\n\n\n
\u81ea\u5907\u86cb\u767d\u9176\u6291\u5236\u5242\u3002RIPA \u88c2\u89e3\u6db2\uff08\u5f3a\uff09\u5728\u4e34\u7528\u524d\u9700\u52a0\u5165\u86cb\u767d\u9176\u6291\u5236\u5242\uff0c\u9632\u6b62\u86cb\u767d\u964d\u89e3\u3002<\/p>\n\n\n\n
\u4ee5\u4e0b\u4f7f\u7528\u65b9\u6cd5\u4e2d\u63d0\u5230\u7684 RIPA \u88c2\u89e3\u6db2(\u5f3a)\u5747\u6307\u5df2\u6dfb\u52a0\u86cb\u767d\u9176\u6291\u5236\u5242\u3002<\/p>\n\n\n\n
\u7ec4\u7ec7\u6837\u54c1<\/strong>\u5b9e\u9a8c<\/strong>\uff1a<\/p>\n\n\n\n
1. \u7ec4\u7ec7\u5757\u7528\u9884\u51b7 PBS\u6d17\u6da4\uff0c\u53bb\u9664\u8840\u6c61\uff0c\u526a\u6210\u7ec6\u5c0f\u788e\u5757\u7f6e\u4e8e\u5300\u6d46\u5668\u4e2d\u3002<\/p>\n\n\n\n
2. \u52a0\u5165 10 \u500d\u7ec4\u7ec7\u4f53\u79ef RIPA \u88c2\u89e3\u6db2(\u5f3a)\u4f4e\u6e29\u5300\u6d46\u6ce8\u610f\uff0cRIPA \u88c2\u89e3\u6db2(\u5f3a)\u7684\u4f7f\u7528\u91cf\u53ef\u6309\u7167\u7ea6\u6bcf 50 mg \u7ec4\u7ec7\u4e0e 1 mL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u6dfb\u52a0\u3002\u5982\u7ec4\u7ec7\u86cb\u767d\u542b\u91cf\u8f83\u4f4e\uff0c\u53ef\u964d\u4f4e\u88c2\u89e3\u6db2\u7684\u7528\u91cf\uff0c\u4ee5\u63d0\u9ad8\u7c97\u63d0\u6eb6\u6db2\u4e2d\u7684\u86cb\u767d\u6d53\u5ea6\u3002<\/p>\n\n\n\n
3. \u5c06\u5300\u6d46\u6db2\u8f6c\u79fb\u81f3 1.5 mL \u79bb\u5fc3\u7ba1\u4e2d\uff0c\u632f\u8361\u3002\u51b0\u6d74 30 min\uff0c\u671f\u95f4\u6bcf 10 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\uff0c\u786e\u4fdd\u7ec4\u7ec7\u7ec6\u80de\u5b8c\u5168\u88c2\u89e3\uff1b<\/p>\n\n\n\n
4. 12000g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/p>\n\n\n\n
\u5bf9\u4e8e\u8d34\u58c1\u7ec6\u80de\u6837\u54c1\uff1a<\/strong><\/strong><\/p>\n\n\n\n
1. \u7528 PBS \u6e05\u6d17\u7ec6\u80de 2-3 \u6b21\uff0c\u6700\u540e\u4e00\u6b21\u5f7b\u5e95\u5438\u5e72\u6b8b\u7559\u6db2\u3002<\/p>\n\n\n\n
2. \u6309\u7167 6 \u5b54\u677f\u6bcf\u5b54\u7ec6\u80de 250 \u03bcL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u5438\u53d6 RIPA \u88c2\u89e3\u6db2(\u5f3a)\u4e8e\u7ec6\u80de\u57f9\u517b\u677f\u3001\u74f6\u5185\uff0c\u53cd\u590d\u6643\u52a8\u57f9\u517b\u677f\u3001\u74f6\uff0c\u4f7f\u88c2\u89e3\u6db2\u4e0e\u7ec6\u80de\u5145\u5206\u63a5\u89e6 3-5 min\u3002<\/p>\n\n\n\n
3. \u7528\u7ec6\u80de\u522e\u5200\u5c06\u7ec6\u80de\u522e\u4e0b\uff0c\u6536\u96c6\u5230\u79bb\u5fc3\u7ba1\u4e2d\u3002<\/p>\n\n\n\n
4. 12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/p>\n\n\n\n
- \n
- <\/li>\n<\/ol>\n\n\n\n
\u5bf9\u4e8e\u60ac\u6d6e\u7ec6\u80de\u6837\u54c1\uff1a<\/strong><\/p>\n\n\n\n
- \n
- \u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\u3002<\/li>\n\n\n\n
- \u6309\u7167 6 \u5b54\u677f\u6bcf\u5b54\u7ec6\u80de 250 \u03bcL \u88c2\u89e3\u6db2\u7684\u6bd4\u4f8b\u5c06\u7ec6\u80de\u6db2\u4e0e RIPA \u88c2\u89e3\u6db2(\u5f3a)\u6df7\u5408\uff0c\u632f\u8361\u3002<\/li>\n\n\n\n
- \u51b0\u6d74 30 min\uff0c\u671f\u95f4\u6bcf 10 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6570\u6b21\uff0c\u786e\u4fdd\u7ec6\u80de\u5b8c\u5168\u88c2\u89e3\u3002<\/li>\n\n\n\n
- 12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/li>\n<\/ol>\n\n\n\n
\u5bf9\u4e8e\u7ec6\u83cc\u6216\u771f\u83cc\u6837\u672c\uff1a<\/strong><\/p>\n\n\n\n
- \n
- \u53d6 1 mL \u83cc\u60ac\u6db2\uff0c\u79bb\u5fc3\u53bb\u4e0a\u6e05\uff0cPBS \u6d17\u6da4\u4e00\u6b21\uff0c\u5145\u5206\u53bb\u9664\u6db2\u4f53\u3002\u6da1\u65cb\u4f7f\u83cc\u4f53\u5c3d\u91cf\u5206\u6563\u3002<\/li>\n\n\n\n
- \u52a0\u5165 100-200 \u03bcL RIPA \u88c2\u89e3\u6db2(\u5f3a)\uff0c\u8f7b\u8f7b\u6da1\u65cb\u4f7f\u83cc\u4f53\u4e0e\u88c2\u89e3\u6db2\u5145\u5206\u6df7\u5300\u3002<\/li>\n\n\n\n
- \u51b0\u6d74 10 min\uff0c\u671f\u95f4\u6bcf 2 min \u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6570\u6b21\uff0c\u786e\u4fdd\u83cc\u4f53\u5b8c\u5168\u88c2\u89e3\u3002<\/li>\n\n\n\n
- 12000 g \u79bb\u5fc3 5 min\uff0c\u6536\u96c6\u4e0a\u6e05\uff0c\u5373\u4e3a\u603b\u86cb\u767d\u6eb6\u6db2\u3002<\/li>\n<\/ol>\n\n\n\n
\u3010\u6ce8\u610f\u4e8b\u9879\u3011<\/strong><\/strong><\/p>\n\n\n\n
- \n
- \u7ec4\u7ec7\u6216\u7ec6\u80de\u88c2\u89e3\u65f6\u82e5\u4f1a\u51fa\u73b0\u7c98\u7a20\u72b6\u3002\u53ef\u7528\u79fb\u6db2\u5668\u53cd\u590d\u5439\u6253\u6216\u632f\u8361\u5668\u632f\u8361\uff0c\u76f4\u81f3\u5448\u6db2\u72b6\u4e3a\u6b62\u3002\u5982\u679c\u4e00\u76f4\u8f83\u7a20\uff0c\u53ef\u518d\u52a0\u5165\u9002\u91cf\u88c2\u89e3\u6db2\u3002<\/li>\n\n\n\n
- \u672c\u8bd5\u5242\u4e0d\u542b\u6709\u86cb\u767d\u9176\u6291\u5236\u5242\uff0c\u9700\u81ea\u5907\u86cb\u767d\u9176\u6291\u5236\u5242\u5e76\u5728\u4e34\u7528\u524d\u52a0\u5165\u3002<\/li>\n\n\n\n
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- <\/li>\n<\/ol>\n\n\n\n
- <\/i><\/a>\u8bf4\u660e\u4e66.pdf<\/span><\/a><\/li><\/ul>