{"id":1112,"date":"2019-12-08T21:56:28","date_gmt":"2019-12-08T13:55:38","guid":{"rendered":"http:\/\/haokebio.com\/1112.html"},"modified":"2024-11-29T16:49:39","modified_gmt":"2024-11-29T08:49:39","slug":"c6cfa8788d","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1112.html","title":{"rendered":"\u539f\u4f4d\u6742\u4ea4(FISH\u4e09\u6807\uff09"},"content":{"rendered":"
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\u539f\u4f4d\u6742\u4ea4(FISH\u4e09\u6807\uff09<\/div>\n<\/div>\n\n\n
Tunel\uff08\u767d\u5149\uff09\u68c0\u6d4b<\/td>200\u5143\/\u5f20<\/td><\/tr>
Tunel\uff08\u8367\u5149\uff09\u68c0\u6d4b<\/td>200\u5143\/\u5f20<\/td><\/tr>
IF Tunel \u53cc\u67d3<\/td>250\u5143\/\u5f20<\/td><\/tr>
Tunel( \u82af\u7247\uff09<\/td>550\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08DAB \u663e\u8272\uff09<\/td>240\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u5355\u6807\uff09<\/td>240\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH\u3001IF \u53cc\u67d3\uff09<\/td>300\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u53cc\u6807\uff09<\/td>300\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u4e09\u6807\uff09<\/td>350\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08\u82af\u7247\uff09<\/td>500 \u5143\u8d77<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001BIO\uff09<\/td>600\u5143\/\u6761<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001DIG\uff09<\/td>1000 \u5143 \/ \u6761<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001FAM\uff09<\/td>1100 \u5143 \/ \u6761<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001Cy3\uff09<\/td>1900 \u5143 \/ \u6761<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001BIO\uff09<\/td>1200 \u5143 \/ \u6761<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001DIG\uff09<\/td>1690 \u5143 \/ \u6761<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001FAM\uff09<\/td>1400 \u5143 \/ \u6761<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001Cy3\uff09<\/td>2500 \u5143 \/ \u6761<\/td><\/tr><\/tbody><\/table>
\u6807\u672c\u8981\u6c42\uff1a<\/strong>\u77f3\u8721\uff0c\u51b0\u5207\uff0c\u7ec6\u80de\u722c\u7247
\u3000\u3000\u4ea7\u54c1\u5468\u671f\uff1a<\/strong>5\u4e2a\u5de5\u4f5c\u65e5\u3002
\u3000\u3000\u9879\u76ee\u4ecb\u7ecd\uff1a<\/strong>
\u3000\u30001. \u539f\u4f4d\u6742\u4ea4\u539f\u7406\uff1a\u539f\u4f4d\u6742\u4ea4\u662f\u6307\u5c06\u7279\u5b9a\u6807\u8bb0\u7684\u5df2\u77e5\u987a\u5e8f\u6838\u9178\u4e3a\u63a2\u9488\u4e0e\u7ec6\u80de\u6216\u7ec4\u7ec7\u5207\u7247\u4e2d\u6838\u9178\u8fdb\u884c\u6742\u4ea4\uff0c\u4ece\u800c\u5bf9\u7279\u5b9a\u6838\u9178\u987a\u5e8f\u8fdb\u884c\u7cbe\u786e\u5b9a\u91cf\u5b9a\u4f4d\u7684\u8fc7\u7a0b\u3002\u539f\u4f4d\u6742\u4ea4\u53ef\u4ee5\u5728\u7ec6\u80de\u6807\u672c\u6216\u7ec4\u7ec7\u6807\u672c\u4e0a\u8fdb\u884c\u3002
\u3000\u30002. \u6240\u9700\u5668\u6750\uff1a
\u3000\u3000\u8131\u6c34\u673aG-JT12s\uff0c\u5305\u57cb\u673aG-L5\/p5,\u75c5\u7406\u5207\u7247\u673aRM2016\uff0c\u51bb\u53f0G-P1\uff0c\u70e4\u7bb1GFL-70\/230,\u7ec4\u7ec7\u644a\u7247\u673aG-KDP\uff0c\u8f7d\u73bb\u7247\u53ca\u76d6\u73bb\u7247\uff0c\u6b63\u7f6e\u8367\u5149\u663e\u5fae\u955c\uff0c\u6210\u50cf\u7cfb\u7edf\uff0c\u6052\u6e29\u7bb1\uff0c\u9ad8\u538b\u706d\u83cc\u9505\uff0c\u79fb\u6db2\u67aa\uff0c\u949f\u6446\u6447\u5e8a\u3002
\u3000\u30003. \u4e3b\u8981\u8bd5\u5242\uff1aDEPC\u3002\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u3002PBS\u7f13\u51b2\u6db2\u300220\u00d7SSC\u6d17\u8131\u6db2\u3002\u86cb\u767d\u9176K\u3002DAPI\u3002\u6297\u8367\u5149\u6dec\u706d\u5c01\u7247\u5242\u3002\u6742\u4ea4\u6db2\u3002
\u3000\u3000\u5b9e\u9a8c\u6b65\u9aa4\uff1a<\/strong>
\u3000\u3000\u7ec6\u80de\u722c\u7247\u8367\u5149\u63a2\u9488\u539f\u4f4d\u6742\u4ea4\u53cc\u6807\u5b9e\u9a8c\u6b65\u9aa4<\/strong>
\u3000\u30001\u3001\u7ec6\u80de\u722c\u7247\u56fa\u5b9a\uff1a<\/strong>\u7ec6\u80de\u722c\u7247\u7f6e\u4e8e4%\u591a\u805a\u7532\u919b(DEPC)\u56fa\u5b9a20min\uff0c\u4e8ePBS(PH7.4)\u4e2d\u5728\u8131\u8272\u6447\u5e8a\u4e0a\u6643\u52a8\u6d17\u6da43\u6b21\uff0c\u6bcf\u6b215min\u3002
\u3000\u30002\u3001\u6d88\u5316\uff1a<\/strong>\u57fa\u56e0\u7b14\u753b\u5708\uff0c\u6839\u636e\u4e0d\u540c\u7ec4\u7ec7\u4e0d\u540c\u6307\u6807\u7279\u6027\uff0c\u6ef4\u52a0\u86cb\u767d\u9176K(20ug\/ml)\u6d88\u53168min\u3002\u7eaf\u6c34\u51b2\u6d17\u540ePBS\u6d173\u6b21\u00d75min\u3002
\u3000\u30003\u3001\u9884\u6742\u4ea4\uff1a<\/strong>\u6ef4\u52a0\u9884\u6742\u4ea4\u6db237\u00b0\u6052\u6e29\u7bb11h\u3002
\u3000\u30004\u3001\u6742\u4ea4\uff1a<\/strong>\u503e\u53bb\u9884\u6742\u4ea4\u6db2\uff0c\u6ef4\u52a0\u542b\u63a2\u94881\u6742\u4ea4\u6db2\uff0c37\u5ea6\u6742\u4ea4\u8fc7\u591c\u3002
\u3000\u30005\u3001\u6742\u4ea4\u540e\u6d17\u6da4\uff1a<\/strong>\u6d17\u53bb\u6742\u4ea4\u6db2\uff0c2\u00d7SSC\uff0c37\u00b0C \u6d1710min\uff0c1\u00d7SSC\uff0c37\u00b0C\u6d172\u00d75min\uff0c0.5\u00d7SSC 37\u00b0\u6d1710min\u3002\u82e5\u975e\u7279\u5f02\u6742\u4ea4\u4f53\u8f83\u591a\uff0c\u53ef\u4ee5\u589e\u52a0\u7532\u9170\u80fa\u6d17\u6da4
\u3000\u30006\u3001\u6742\u4ea4\uff1a<\/strong>\u503e\u53bb\u9884\u6742\u4ea4\u6db2\uff0c\u6ef4\u52a0\u542b\u63a2\u94882\u6742\u4ea4\u6db2\uff0c37\u5ea6\u6742\u4ea4\u8fc7\u591c\u3002
\u3000\u30007\u3001DAPI\u590d\u67d3\u6838\uff1a<\/strong>\u5207\u7247\u6ef4\u52a0DAPI\u67d3\u6db2\uff0c\u907f\u5149\u5b75\u80b28min\uff0c\u51b2\u6d17\u540e\u6ef4\u52a0\u6297\u8367\u5149\u6dec\u706d\u5c01\u7247\u5242\u5c01\u7247\u3002
\u3000\u30008\u3001\u955c\u68c0\u62cd\u7167\uff1a<\/strong>\u5207\u7247\u4e8e\u5c3c\u5eb7\u6b63\u7f6e\u8367\u5149\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u5e76\u91c7\u96c6\u56fe\u50cf\u3002(\u7d2b\u5916\u6fc0\u53d1\u6ce2\u957f330-380nm\uff0c\u53d1\u5c04\u6ce2\u957f420nm,\u53d1\u84dd\u5149;FAM(488)\u7eff\u5149\u6fc0\u53d1\u6ce2\u957f465-495nm\uff0c\u53d1\u5c04\u6ce2\u957f515-555 nm\uff0c\u53d1\u7eff\u5149;CY3\u7ea2\u5149\u6fc0\u53d1\u6ce2\u957f510-560\uff0c\u53d1\u5c04\u6ce2\u957f590nm\uff0c\u53d1\u7ea2\u5149\u3002)
\u3000\u3000\u7ec6\u80de\u722c\u7247\u8367\u5149\u63a2\u9488\u539f\u4f4d\u6742\u4ea4\u53cc\u6807\u5b9e\u9a8c\u7ed3\u679c\u5224\u8bfb<\/strong>
\u3000\u3000DAPI\u67d3\u51fa\u6765\u7684\u7ec6\u80de\u6838\u5728\u7d2b\u5916\u7684\u6fc0\u53d1\u4e0b\u4e3a\u84dd\u8272\uff0c\u9633\u6027\u8868\u8fbe\u4e3a\u76f8\u5e94\u8367\u5149\u7d20\u6807\u8bb0\u7684\u8367\u5149\u3002FAM(488)\u4e3a\u7eff\u5149\uff0ccy3\u4e3a\u7ea2\u5149\u3002mRNA\u539f\u4f4d\u6742\u4ea4\u663e\u793a\u7ed3\u679c\u7406\u8bba\u4e3a\u80de\u6d46\u9633\u6027\uff0c\u5c11\u6570\u6838\u9633\u6027\u5c5e\u6b63\u5e38\u3002micRNA\u4e0elncRNA\u4e0d\u540c\u6307\u6807\u8868\u8fbe\u5b9a\u4f4d\u4e0d\u540c\u3002\u6839\u636e\u8868\u8fbe\u91cf\u4e0d\u540c\u8367\u5149\u4eae\u5ea6\u6709\u5f3a\u5f31\u3002<\/figcaption><\/figure>\n\n\n\n

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