{"id":1107,"date":"2019-12-08T21:56:25","date_gmt":"2019-12-08T13:55:35","guid":{"rendered":"http:\/\/haokebio.com\/1107.html"},"modified":"2024-11-29T17:20:52","modified_gmt":"2024-11-29T09:20:52","slug":"2509eb8f7f","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1107.html","title":{"rendered":"\u8367\u5149\u539f\u4f4d\u6742\u4ea4\uff08FISH\u3001IF \u53cc\u67d3\uff09"},"content":{"rendered":"
\n
\n

\u8367\u5149\u539f\u4f4d\u6742\u4ea4\uff08FISH\u3001IF \u53cc\u67d3\uff09<\/p>\n<\/div>\n<\/div>\n\n\n

Tunel\uff08\u767d\u5149\uff09\u68c0\u6d4b<\/td>200\u5143\/\u5f20<\/td><\/tr>
Tunel\uff08\u8367\u5149\uff09\u68c0\u6d4b<\/td>200\u5143\/\u5f20<\/td><\/tr>
IF Tunel \u53cc\u67d3<\/td>250\u5143\/\u5f20<\/td><\/tr>
Tunel( \u82af\u7247\uff09<\/td>550\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08DAB \u663e\u8272\uff09<\/td>240\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u5355\u6807\uff09<\/td>240\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH\u3001IF \u53cc\u67d3\uff09<\/td>300\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u53cc\u6807\uff09<\/td>300\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u4e09\u6807\uff09<\/td>350\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08\u82af\u7247\uff09<\/td>500\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001BIO\uff09<\/td>600\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001DIG\uff09<\/td>1000\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001FAM\uff09<\/td>1100\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001Cy3\uff09<\/td>1900\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001BIO\uff09<\/td>1200\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001DIG\uff09<\/td>1960\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001FAM\uff09<\/td>1400\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001Cy3\uff09<\/td>2500\u5143\/\u5f20<\/td><\/tr><\/tbody><\/table>
\u9879\u76ee\u4ecb\u7ecd<\/strong>
\u3000\u3000\u539f\u4f4d\u6742\u4ea4\u4e0e\u514d\u75ab\u8367\u5149\u539f\u7406\uff1a\u539f\u4f4d\u6742\u4ea4\u662f\u6307\u5c06\u7279\u5b9a\u6807\u8bb0\u7684\u5df2\u77e5\u987a\u5e8f\u6838\u9178\u4e3a\u63a2\u9488\u4e0e\u7ec6\u80de\u6216\u7ec4\u7ec7\u5207\u7247\u4e2d\u6838\u9178\u8fdb\u884c\u6742\u4ea4\uff0c\u4ece\u800c\u5bf9\u7279\u5b9a\u6838\u9178\u987a\u5e8f\u8fdb\u884c\u7cbe\u786e\u5b9a\u91cf\u5b9a\u4f4d\u7684\u8fc7\u7a0b\u3002\u539f\u4f4d\u6742\u4ea4\u53ef\u4ee5\u5728\u7ec6\u80de\u6807\u672c\u6216\u7ec4\u7ec7\u6807\u672c\u4e0a\u8fdb\u884c\u3002\u514d\u75ab\u8367\u5149\u5c31\u662f\u5c06\u4e0d\u5f71\u54cd\u6297\u539f\u6297\u4f53\u6d3b\u6027\u7684\u8367\u5149\u8272\u7d20\u6807\u8bb0\u5728\u6297\u4f53\u6216\u6297\u539f\u4e0a\uff0c\u4e0e\u5176\u76f8\u5e94\u7684\u6297\u539f\u6216\u6297\u4f53\u7ed3\u5408\u540e\uff0c\u5728\u8367\u5149\u663e\u5fae\u955c\u4e0b\u5448\u73b0\u4e00\u79cd\u7279\u5f02\u6027\u8367\u5149\u53cd\u5e94\u3002
\u3000\u3000\u5e94\u7528<\/strong>
\u3000\u3000\u901a\u8fc7\u539f\u4f4d\u6742\u4ea4\u53ca\u514d\u75ab\u8367\u5149\u53cc\u67d3\uff0c\u53ef\u4ee5\u5728\u7ec4\u7ec7\u7ec6\u80de\u4e2d\u5bf9\u6838\u9178\u5206\u5b50\u548c\u86cb\u767d\u6307\u6807\u540c\u65f6\u8fdb\u884c\u5171\u5b9a\u4f4d\u3001\u4ee5\u53ca\u5b9a\u6027\u5206\u6790\uff0c\u53ef\u4ee5\u7528\u6765\u4ece\u5171\u5b9a\u4f4d\u89d2\u5ea6\u63d0\u793a\u6838\u9178\u548c\u86cb\u767d\u5b58\u5728\u76f8\u4e92\u4f5c\u7528\u3002
\u3000\u3000\u5b9e\u9a8c\u6d41\u7a0b<\/strong>
\u3000\u3000\u77f3\u8721\u5207\u7247\u8367\u5149\u63a2\u9488\u539f\u4f4d\u6742\u4ea4\u4e0e\u514d\u75ab\u8367\u5149\u53cc\u6807\u5b9e\u9a8c\u6b65\u9aa4
\u3000\u3000
1. \u7ec4\u7ec7\u56fa\u5b9a\u3001\u8131\u6c34\u3001\u5207\u7247\u3001\u77f3\u8721\u5207\u7247\u8131\u8721\u81f3\u6c34;
\u3000
2. \u6d88\u5316\uff1a\u5207\u7247\u4e8e\u4fee\u590d\u6db2\u4e2d\u716e\u6cb810-15\u5206\u949f\uff0c\u6ef4\u52a0\u86cb\u767d\u9176K\u6d88\u5316;
\u3000\u3000
3. \u9884\u6742\u4ea4\uff1a\u6ef4\u52a0\u9884\u6742\u4ea4\u6db2\uff0c37\u00b0C\u5b75\u80b21h\u3002
\u3000\u3000
4. \u6742\u4ea4\uff1a\u503e\u53bb\u9884\u6742\u4ea4\u6db2\uff0c\u6ef4\u52a0\u542b\u63a2\u9488 \u6742\u4ea4\u6db2\u6d53\u5ea6 \uff0c\u6052\u6e29\u7bb1 \u5ea6\u6742\u4ea4\u8fc7\u591c\u3002
\u3000\u3000
5. \u6742\u4ea4\u540e\u6d17\u6da4\uff1aSSC\u6d17\u6da4;
\u3000\u3000
6. \u5b75\u80b2\u4e00\u6297\u3001\u4e8c\u6297\uff1a\u6ef4\u52a0\u4e00\u6297\uff0c4\u2103\u8fc7\u591c\uff0c\u540ePBS\u6d173\u00d75min;\u6ef4\u52a0\u76f8\u5e94\u4e8c\u6297\uff0c\u5ba4\u6e29\u5b75\u80b250min\uff0cPBS\u6d173\u00d75min;
\u3000\u3000
7. DAPI\u590d\u67d3\u6838\uff1a\u5207\u7247\u6ef4\u52a0DAPI\u67d3\u6db2\uff0c\u907f\u5149\u5b75\u80b28min;
\u3000\u3000
8. \u955c\u68c0\u62cd\u7167;
\u3000\u3000
\u6837\u54c1\u4fdd\u5b58\u8fd0\u8f93<\/strong>
\u3000\u7ec4\u7ec7\u51b0\u51bb\u5207\u7247<\/strong>
\u3000\u7ec4\u7ec7\u5207\u53d6\u9700\u8981\u90e8\u4f4d\uff0c\u539a\u5ea6\u57282mm \u5de6\u53f3, PBS\u6f02\u6d17\uff0c\u5e72\u51b0\u8fd0\u8f93\u6216\u7acb\u5373\u7f6e\u4e8e\u56fa\u5b9a\u6db2\u4e2d\u3002\u7ec4\u7ec7\u56fa\u5b9a\u6709\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2
(\u52a8\u7269)\u3001\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u690d\u7269)\u3001\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u8f83\u5ae9\u690d\u7269)\uff0c\u5ba4\u6e29\u56fa\u5b9a12\u5c0f\u65f6(\u6839\u636e\u7ec4\u7ec7\u5927\u5c0f)\u4ee5\u4e0a\uff0c\u690d\u7269\u7ec4\u7ec7\u56fa\u5b9a\u65f6\u9700\u5e38\u6e29\u62bd\u771f\u7a7a30min\uff0c\u518d\u7528\u7eaf\u6c34\u6216PBS\u6e05\u6d17\u4e24\u6b21\u540e\uff0c \u653e\u516515%\u8517\u7cd6\u6eb6\u6db2\u4e2d\uff0c4\u2103\u8131\u6c348\u5c0f\u540e\uff0c\u636230%\u8517\u7cd6\u6eb6\u6db24\u2103\u8fc7\u591c\u3002\u51b0\u5207\u65f6\u4f69\u6234\u624b\u5957\u3002\u51b0\u51bb\u5207\u7247\u653e-20\u2103\u6216-80\u2103\u4fdd\u5b58\u5907\u7528\u3002
\u3000\u7ec4\u7ec7\u77f3\u8721\u5207\u7247<\/strong>
\u3000\u7ec4\u7ec7\u5207\u53d6\u9700\u8981\u90e8\u4f4d\uff0c\u539a\u5ea6\u57282mm \u5de6\u53f3, PBS\u6f02\u6d17\u540e\u7acb\u5373\u56fa\u5b9a\u3002\u52a8\u7269\u7ec4\u7ec7\u7528\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u52a8\u7269)\uff0c\u690d\u7269\u7ec4\u7ec7\u7528\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u690d\u7269)\u6216\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2(\u8f83\u5ae9\u690d\u7269)\uff0c\u690d\u7269\u7ec4\u7ec7\u56fa\u5b9a\u65f6\u9700\u5e38\u6e29\u62bd\u771f\u7a7a30min\uff0c\u56fa\u5b9a12h\u4ee5\u4e0a\u3002\u56fa\u5b9a\u5b8c\u6210\u540e\u8131\u6c34\u3001\u5305\u57cb\u3001\u5207\u7247\u3002\u68af\u5ea6\u9152\u7cbe\u914d\u5236\u9700\u7528DEPC\u6c34\u3002\u77f3\u8721\u5e38\u89c4\u4fdd\u5b58\u5907\u7528\u3002
\u3000\u6807\u672c\u8fd0\u8f93<\/strong>
\u3000\u51b0\u51bb\u5207\u7247\uff1a\u6807\u672c\u653e\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u4e2d\uff0c\u5e38\u6e29\u8fd0\u8f93;\u51b0\u51bb\u5207\u7247-20\u2103\u8fd0\u8f93;\u65b0\u9c9c\u7ec4\u7ec7\u5e72\u51b0\u8fd0\u8f93\u3002\u4ea4\u63a5\u5355\u968f\u6837\u672c\u4e00\u8d77\uff0c\u5355\u72ec\u5c01\u88c5\u3002
\u3000\u77f3\u8721\u5207\u7247\uff1a\u6807\u672c\u653e\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u4e2d\uff0c\u5e38\u6e29\u8fd0\u8f93;\u77f3\u8721\u5207\u7247\u5e38\u6e29\u8fd0\u8f93\u3002\u4ea4\u63a5\u5355\u968f\u6837\u672c\u4e00\u8d77\uff0c\u5355\u72ec\u5c01\u88c5\u3002
\u3000\u7ec6\u80de\u722c\u7247\uff1a\u7ec6\u80de\u722c\u7247\u52a0\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u540e\u5bc6\u5c014\u2103\u8fd0\u8f93\u3002\u4ea4\u63a5\u5355\u968f\u6837\u672c\u4e00\u8d77\uff0c\u5355\u72ec\u5c01\u88c5\u3002
\u3000\u5b9e\u9a8c\u6ce8\u610f\u4e8b\u9879\uff1a<\/strong>
\u3000\u3000
1. mRNA\u4e3a\u68c0\u6d4b\u5bf9\u8c61\u65f6\uff0c\u9700\u4e25\u683c\u8981\u6c42\u5b9e\u9a8c\u73af\u5883\u7ecf\u65e0RNA\u9176\u5904\u7406;
\u3000\u3000
2. FISH\u5207\u7247\u4fdd\u5b58\u65f6\u95f4\u8f83\u77ed\uff0c\u5e94\u5c3d\u5feb\u53d6\u56fe;
\u3000\u3000
3. \u4e0d\u540c\u7684\u6297\u4f53\u4e0e\u539f\u4f4d\u6742\u4ea4\u8fdb\u884c\u53cc\u6807\u65f6\uff0c\u53ef\u80fd\u4f1a\u6709\u5f71\u54cd\uff0c\u5fc5\u8981\u65f6\u8fdb\u884c\u4e00\u5b9a\u7684\u5b9e\u9a8c\u6b65\u9aa4\u8c03\u6574\uff0c\u4ee5\u907f\u514d\u76f8\u4e92\u4e4b\u95f4\u7684\u5f71\u54cd;<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"

\u8367\u5149\u539f\u4f4d\u6742\u4ea4\uff08FISH\u3001IF \u53cc\u67d3\uff09 Tunel\uff08\u767d\u5149\uff09\u68c0\u6d4b 200\u5143\/\u5f20 Tunel\uff08\u8367\u5149\uff09\u68c0\u6d4b 200\u5143\/ […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1107","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["4249"],"_edit_lock":["1732872052:2"],"_edit_last":["2"],"catce":["sidebar-widgets4"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1107"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=1107"}],"version-history":[{"count":1,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1107\/revisions"}],"predecessor-version":[{"id":13320,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1107\/revisions\/13320"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=1107"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=1107"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=1107"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}