{"id":1106,"date":"2019-12-08T21:56:23","date_gmt":"2019-12-08T13:55:33","guid":{"rendered":"http:\/\/haokebio.com\/1106.html"},"modified":"2024-11-29T17:22:56","modified_gmt":"2024-11-29T09:22:56","slug":"19bbc25419","status":"publish","type":"post","link":"https:\/\/www.haokebio.com\/1106.html","title":{"rendered":"\u539f\u4f4d\u6742\u4ea4\uff08FISH,\u5355\u6807\uff09"},"content":{"rendered":"
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\u539f\u4f4d\u6742\u4ea4\uff08FISH\uff0c\u5355\u6807\uff09<\/strong><\/p>\n<\/div>\n<\/div>\n\n\n

Tunel\uff08\u767d\u5149\uff09\u68c0\u6d4b<\/td>200\u5143\/\u5f20<\/td><\/tr>
Tunel\uff08\u8367\u5149\uff09\u68c0\u6d4b<\/td>200\u5143\/\u5f20<\/td><\/tr>
IF Tunel \u53cc\u67d3<\/td>250\u5143\/\u5f20<\/td><\/tr>
Tunel( \u82af\u7247\uff09<\/td>550\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08DAB \u663e\u8272\uff09<\/td>240\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u5355\u6807\uff09<\/td>240\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH\u3001IF \u53cc\u67d3\uff09<\/td>300\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u53cc\u6807\uff09<\/td>300\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08FISH \u4e09\u6807\uff09<\/td>350\u5143\/\u5f20<\/td><\/tr>
\u539f\u4f4d\u6742\u4ea4\uff08\u82af\u7247\uff09<\/td>500\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001BIO\uff09<\/td>600\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001DIG\uff09<\/td>1000\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001FAM\uff09<\/td>1100\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e00\u7aef\u6807\u8bb0\u3001Cy3\uff09<\/td>1900\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001BIO\uff09<\/td>1200\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001DIG\uff09<\/td>1960\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001FAM\uff09<\/td>1400\u5143\/\u5f20<\/td><\/tr>
\u63a2\u9488\u8bbe\u8ba1\u5408\u6210\uff08\u4e24\u7aef\u6807\u8bb0\u3001Cy3\uff09<\/td>2500\u5143\/\u5f20<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

\u9879\u76ee\u4ecb\u7ecd\uff1a<\/strong><\/p>\n\n\n\n

\u3000\u30001.\u539f\u4f4d\u6742\u4ea4\u539f\u7406\uff1a\u539f\u4f4d\u6742\u4ea4\u662f\u6307\u5c06\u7279\u5b9a\u6807\u8bb0\u7684\u5df2\u77e5\u987a\u5e8f\u6838\u9178\u4e3a\u63a2\u9488\u4e0e\u7ec6\u80de\u6216\u7ec4\u7ec7\u5207\u7247\u4e2d\u6838\u9178\u8fdb\u884c\u6742\u4ea4\uff0c\u4ece\u800c\u5bf9\u7279\u5b9a\u6838\u9178\u987a\u5e8f\u8fdb\u884c\u7cbe\u786e\u5b9a\u91cf\u5b9a\u4f4d\u7684\u8fc7\u7a0b\u3002\u539f\u4f4d\u6742\u4ea4\u53ef\u4ee5\u5728\u7ec6\u80de\u6807\u672c\u6216\u7ec4\u7ec7\u6807\u672c\u4e0a\u8fdb\u884c\u3002<\/p>\n\n\n\n

\u3000\u30002.\u6240\u9700\u5668\u6750\uff1a<\/p>\n\n\n\n

\u3000\u3000\u8131\u6c34\u673aG-JT12s\uff0c\u5305\u57cb\u673aG-L5\/p5,\u75c5\u7406\u5207\u7247\u673aRM2016\uff0c\u51bb\u53f0G-P1\uff0c\u70e4\u7bb1GFL-70\/230,\u7ec4\u7ec7\u644a\u7247\u673aG-KDP\uff0c\u8f7d\u73bb\u7247\u53ca\u76d6\u73bb\u7247\uff0c\u6b63\u7f6e\u8367\u5149\u663e\u5fae\u955c\uff0c\u6210\u50cf\u7cfb\u7edf\uff0c\u6052\u6e29\u7bb1\uff0c\u9ad8\u538b\u706d\u83cc\u9505\uff0c\u79fb\u6db2\u67aa\uff0c\u949f\u6446\u6447\u5e8a\u3002<\/p>\n\n\n\n

\u3000\u30003.\u4e3b\u8981\u8bd5\u5242\uff1aDEPC\u3002\u539f\u4f4d\u6742\u4ea4\u56fa\u5b9a\u6db2\u3002PBS\u7f13\u51b2\u6db2\u300220\u00d7SSC\u6d17\u8131\u6db2\u3002\u86cb\u767d\u9176K\u3002DAPI\u3002\u6297\u8367\u5149\u6dec\u706d\u5c01\u7247\u5242\u3002\u6742\u4ea4\u6db2\u3002<\/p>\n\n\n\n

\u3000\u3000\u77f3\u8721\u5207\u7247\u8367\u5149\u63a2\u9488\u539f\u4f4d\u6742\u4ea4\u5b9e\u9a8c\u6b65\u9aa4<\/strong><\/p>\n\n\n\n

\u3000\u30001\u3001\u7ec4\u7ec7\u56fa\u5b9a\uff1a\u7ec4\u7ec7\u53d6\u51fa\u6d17\u51c0\u540e\u7acb\u5373\u653e\u5165\u56fa\u5b9a\u6db2(DEPC\u6c34\u914d\u5236)\u4e2d\u56fa\u5b9a2-12h\u3002<\/p>\n\n\n\n

\u3000\u30002\u3001\u8131\u6c34\uff1a\u7ec4\u7ec7\u56fa\u5b9a\u5b8c\u6210\u540e\u7ecf\u68af\u5ea6\u9152\u7cbe\u8131\u6c34\u540e\u6d78\u8721\uff0c\u5305\u57cb\u3002<\/p>\n\n\n\n

\u3000\u30003\u3001\u5207\u7247\uff1a\u77f3\u8721\u7ecf\u5207\u7247\u673a\u5207\u7247\uff0c\u644a\u7247\u673a\u635e\u7247\uff0c62\u00b0\u70e4\u7bb1\u70e4\u72472h\u3002<\/p>\n\n\n\n

\u3000\u30004\u3001\u77f3\u8721\u5207\u7247\u8131\u8721\u81f3\u6c34\uff1a\u4f9d\u6b21\u5c06\u5207\u7247\u653e\u5165\u4e8c\u7532\u82ef\u216015min-\u4e8c\u7532\u82ef\u216115min-\u65e0\u6c34\u4e59\u9187\u21605min-\u65e0\u6c34\u4e59\u9187\u21615min-85%\u9152\u7cbe5min-75%\u9152\u7cbe5min-DEPC\u6c34\u6d17\u3002<\/p>\n\n\n\n

\u3000\u30005\u3001\u6d88\u5316\uff1a\u6839\u636e\u7ec4\u7ec7\u56fa\u5b9a\u65f6\u95f4\u957f\u77ed\uff0c\u5207\u7247\u4e8e\u4fee\u590d\u6db2\u4e2d\u716e\u6cb810-15\u5206\u949f\uff0c\u81ea\u7136\u51b7\u5374\u3002\u540e\u57fa\u56e0\u7b14\u753b\u5708\uff0c\u6839\u636e\u4e0d\u540c\u7ec4\u7ec7\u4e0d\u540c\u6307\u6807\u7279\u6027\uff0c\u6ef4\u52a0\u86cb\u767d\u9176K(20ug\/ml) 37\u00b0\u6d88\u5316min\u3002\u7eaf\u6c34\u51b2\u6d17\u540ePBS\u6d173\u6b21\u00d75min\u3002<\/p>\n\n\n\n

\u3000\u30006\u3001\u9884\u6742\u4ea4\uff1a\u6ef4\u52a0\u9884\u6742\u4ea4\u6db2\uff0c37\u00b0C\u5b75\u80b21h\u3002<\/p>\n\n\n\n

\u3000\u30007\u3001\u6742\u4ea4\uff1a\u503e\u53bb\u9884\u6742\u4ea4\u6db2\uff0c\u6ef4\u52a0\u542b\u63a2\u9488\u6742\u4ea4\u6db2, \u6d53\u5ea6\uff0c\u6052\u6e29\u7bb1\u5ea6\u6742\u4ea4\u8fc7\u591c\u3002<\/p>\n\n\n\n

\u3000\u30008\u3001\u6742\u4ea4\u540e\u6d17\u6da4\uff1a\u6d17\u53bb\u6742\u4ea4\u6db2\uff0c2\u00d7SSC\uff0c37\u00b0C \u6d1710min\uff0c1\u00d7SSC\uff0c37\u00b0C\u6d172\u00d75min\uff0c0.5\u00d7SSC\u5ba4\u6e29\u6d1710min\u3002\u82e5\u975e\u7279\u5f02\u6742\u4ea4\u4f53\u8f83\u591a\uff0c\u53ef\u4ee5\u589e\u52a0\u7532\u9170\u80fa\u6d17\u6da4\u3002<\/p>\n\n\n\n

\u3000\u30009\u3001DAPI\u590d\u67d3\u6838\uff1a\u5207\u7247\u6ef4\u52a0DAPI\u67d3\u6db2\uff0c\u907f\u5149\u5b75\u80b28min\uff0c\u51b2\u6d17\u540e\u6ef4\u52a0\u6297\u8367\u5149\u6dec\u706d\u5c01\u7247\u5242\u5c01\u7247\u3002<\/p>\n\n\n\n

\u3000\u300010.\u955c\u68c0\u62cd\u7167\uff1a\u5207\u7247\u4e8e\u5c3c\u5eb7\u6b63\u7f6e\u8367\u5149\u663e\u5fae\u955c\u4e0b\u89c2\u5bdf\u5e76\u91c7\u96c6\u56fe\u50cf\u3002(\u7d2b\u5916\u6fc0\u53d1\u6ce2\u957f330-380nm\uff0c\u53d1\u5c04\u6ce2\u957f420nm,\u53d1\u84dd\u5149;FAM(488)\u7eff\u5149\u6fc0\u53d1\u6ce2\u957f465-495nm\uff0c\u53d1\u5c04\u6ce2\u957f515-555 nm\uff0c\u53d1\u7eff\u5149;CY3\u7ea2\u5149\u6fc0\u53d1\u6ce2\u957f510-560\uff0c\u53d1\u5c04\u6ce2\u957f590nm\uff0c\u53d1\u7ea2\u5149\u3002)DAPI\u67d3\u6838\uff0c\u4e3a\u84dd\u5149\u3002<\/p>\n\n\n\n

\u3000\u3000\u6ce8\u610f\u4e8b\u9879\uff1a<\/strong><\/p>\n\n\n\n

\u3000\u30001.1.mRNA\u4e3a\u68c0\u6d4b\u5bf9\u8c61\u65f6\uff0c\u9700\u4e25\u683c\u8981\u6c42\u5b9e\u9a8c\u73af\u5883\u7ecf\u65e0RNA\u9176\u5904\u7406\u3002<\/p>\n\n\n\n

\u3000\u30002.FISH\u5207\u7247\u4fdd\u5b58\u65f6\u95f4\u8f83\u77ed\uff0c\u5e94\u5c3d\u5feb\u53d6\u56fe\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u539f\u4f4d\u6742\u4ea4\uff08FISH\uff0c\u5355\u6807\uff09 Tunel\uff08\u767d\u5149\uff09\u68c0\u6d4b 200\u5143\/\u5f20 Tunel\uff08\u8367\u5149\uff09\u68c0\u6d4b 200\u5143\/\u5f20 IF […]<\/p>\n","protected":false},"author":1,"featured_media":7870,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"themepark_post_bcolor":"#f5f5f5","themepark_post_width":"1022px","themepark_post_img":"","themepark_post_img_po":"left","themepark_post_img_re":false,"themepark_post_img_cover":false,"themepark_post_img_fixed":false,"themepark_post_hide_title":false,"themepark_post_main_b":"","themepark_post_main_p":100,"themepark_paddingblock":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-1106","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-uncategorized"],"metadata":{"is_bp":["1"],"views":["890"],"_edit_lock":["1732872177:2"],"_edit_last":["2"],"catce":["sidebar-widgets4"],"_thumbnail_id":["7870"]},"medium_url":null,"thumbnail_url":null,"full_url":null,"_links":{"self":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1106"}],"collection":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/comments?post=1106"}],"version-history":[{"count":1,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1106\/revisions"}],"predecessor-version":[{"id":13322,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/posts\/1106\/revisions\/13322"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/"}],"wp:attachment":[{"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/media?parent=1106"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/categories?post=1106"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.haokebio.com\/wp-json\/wp\/v2\/tags?post=1106"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}