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GFAP兔單克隆抗體

GFAP兔單克隆抗體

  • 價(jià)格￥600
  • 規(guī)格100ul
  • 貨號(hào)HKZ150179
  • 單位支
  • 品牌HaoKebio

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HaoKebioHKZ150179GFAP兔單克隆抗體100ul支600
GFAP
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
Catalog# BX50056?Clone# ?BP6061
Predicted?Molecular Wt:
50kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P14136
Background:
GFAP?(Glial fibrillary acidic?protein)?is an?intermediate filament?protein. The?protein?is the smallest?(8?nm)?of the?intermediate?filament?proteins with a?molecular weight of?about?50?kDa.
In the central?nervous system, GFAP?is expressed?in?astrocytes?andependymal?cells?but?not?in?other?glial?cells.?However,
immature oligodendrocytes and?immature choroid?plexus cells?may?be GFAP?positive.?In?the?peripheral?nervous?system?enteric?Schwann cells and satellite cells?of?human?sensory?ganglia?express?GFAP.?Outside?the?nervous?system,?GFAP?is?seen?in
myoepithelial cells and chondroblasts.?In tumor tissues,?astrocytoma,?ependymoma, glioblastoma,?and?oligodendroglioma?are?almost always?positive.?Plexus carcinoma, ganglioglioma and?primitive?neuroectodermal tumors?(PNET:?neuroblastoma?a.o.)?????express GFAP to a varying extent.?Schwannoma?and?neurofibroma frequently?express?GFAP.?Chondroma,?chondrosarcoma
and?pleomorphic adenoma are also GFAP?positive?in?most?cases.?A?few?carcinomas?(especially?from?lung?and?breast)?may?express GFAP?in?paraganglioma GFAP?may?be detected?in sustentacular?cells.
GFAP?is?used to differentiate astrocytoma from?non-glial cell?tumors.
Subcellular?location:
Cytoplasm
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to GFAP?residues
within?aa332-432 of??GFAP?was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200
Background?References:
1. ?McLendon?RE, et?al.?Brain?Tumor?Pathol.?2002;19(2):51-8.?Review.
2. ?Matyja?E, et?al.?Folia?Neuropathol.?2001;39(1):19-26.
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections analysis?of
astrocytoma tissue
labelling GFAP?with
BP6061.?Heat?mediated antigen?retrieval?was
performed?using
Tris/EDTA?buffer?pH?9.0

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