MSH6兔單克隆抗體

價格￥600
規(guī)格100ul
貨號HKZ150126
單位支
品牌HaoKebio

產(chǎn)品介紹

產(chǎn)品參數(shù)

資料下載

品牌	商品編碼	商品名稱	規(guī)格	單位	單價
HaoKebio	HKZ150126	MSH6兔單克隆抗體	100ul	支	600

MSH6
Recombinant?Rabbit?Monoclonal Antibody?Product?Datasheet
?
Catalog# ?BX50003?Clone# ?BP6007
?
Predicted?Molecular Wt:
153kDa
Purity: ??ProA?affinity?purified?IgG
Species Cross-reactivity:
Human
Form: ??Liquid
Applications:
IHC-P
Swissprot?ID: ??P52701
?

Background:
MSH6?is a?mismatch?repair?protein which?is deficient?in a?high?proportion?of?patients with?microsatellite?instability?(MSI-H).?It?has?been suggested that the deficiencies?in?DNA?mismatch?repair?protein(s) can?be?seen?in?some?malignancies?such?as?hereditary
nonpolyposis colorectal cancer?(HNPCC) and endometrial?cancer.
?
MSH6 expressed?in?all?proliferating cells?participate?in?repair of?base-base?mismatch, that?occur?during?DNA?replication.?Loss?of
MSH6 expression?leads to an accumulation?of?DNA?replication?errors?in the?proliferating?cells,?particularly?in?areas?of the?genome?with short?repetitive?nucleotide sequences, a?phenomenon?known as?microsatellite?instability?(MSI).
?
MSH6 always?used as?panel?with?MLH1,?MSH2,?PMS2, and?may?be?useful to?aid?in?identifying?the?most?probable?gene?responsible?for?the?MSI.
?

Subcellular?location:
Nucleus
Recommended?method:
Heat?induced epitope?retrieval with Tris-EDTA?buffer???(pH?9.0),?primary?antibody?incubate?at?RT?(18°C-25°C)?for?30?minutes.
Immunogen:
Synthetic?peptide corresponding to?MSH6?residues?????within?aa1‐100 of?MSH6 was?used?as?an?immunogen.
Storage?Buffer:
PBS?59%, Sodium?azide?0.01%,?Glycerol?40%,?BSA 0.05%.
Storage conditions:
-25°C?to?-18°C
Storage instructions:
Shipped on?blue?ice.?Upon delivery,?aliquot,?and?store?at?-25°C?to?-18°C. Avoid?freeze?/?thaw?cycles.
Recommended?Dilutions:
IHC-P:?????1:100-1:200

?
Immunohistochemistry?(Formalin/PFA-fixed
paraffin-embedded
sections)?analysis of?human?colon tissue?labelling?MSH6?with?BP6007.?Heat
mediated?antigen?retrieval?was?performed?using
Tris/EDTA?buffer?pH?9.0
Background?References:
1. ?Blackwell?LJ.,?et?al. J?Biol?Chem.?1998?Nov 27;273(48):32055-62.