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Cyclin D3兔多克隆抗體

Cyclin D3兔多克隆抗體

  • 價格￥2800
  • 規(guī)格200ul
  • 貨號HK210122L
  • 單位支
  • 品牌HaoKebio

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HaoKebioHK210122LCyclin D3兔多克隆抗體200ul支2800

Applications

Tested Applications:
IF, IHC, IP, WB,ELISA

Cited Applications:
IHC, WB
Species Specificity:
human, rat

Cited Species:
human, mouse, rat

Positive WB detected in:
C6 cells, K-562 cells, Jurkat cells, HeLa cells

Positive IP detected in:
K-562 cells

Positive IHC detected in:
human pancreas tissue
Note: suggested antigen retrieval with?TE buffer pH 9.0;?(*) Alternatively, antigen retrieval may be performed with?citrate buffer pH 6.0

Positive IF detected in:
HeLa cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500
Product Information

Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment.?Aliquoting is unnecessary for -20oC storage.
Immunogen Information

Full name:
cyclin D3

Calculated molecular weight:
33 kDa

Observed molecular weight:
33 kDa

Gene symbol
CCND3

Synonyms
CCND3, cyclin D, Cyclin D3, G1/S specific cyclin D3
Background
cyclin D3 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation.

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